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Molecules 2017, 22(7), 1055;

Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars

National Institute of Agricultural Science, RDA, Jeonju 54874, Korea
USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Received: 7 June 2017 / Revised: 22 June 2017 / Accepted: 22 June 2017 / Published: 24 June 2017
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The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 16 standard wheat cultivars with known HMW-GS compositions. Subunits that were not identified unambiguously by RP-HPLC were distinguished by SDS-PAGE or inferred from association with linked subunits. The method was used to verify the allelic compositions of 32 Korean wheat cultivars previously determined using SDS-PAGE and to assess the compositions of six new Korean cultivars. Three cultivars contained subunits that were identified incorrectly in the earlier analysis. The improved RP-HPLC method combined with conventional SDS-PAGE provides for accurate, efficient and reliable identification of HMW-GS and will contribute to efforts to improve wheat end-use quality. View Full-Text
Keywords: allelic analysis; high-molecular-weight glutenin subunit; RP-HPLC; SDS-PAGE allelic analysis; high-molecular-weight glutenin subunit; RP-HPLC; SDS-PAGE

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Jang, Y.-R.; Beom, H.-R.; Altenbach, S.B.; Lee, M.-K.; Lim, S.-H.; Lee, J.-Y. Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars. Molecules 2017, 22, 1055.

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