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Molecules 2017, 22(6), 976;

Inhibitory Effects of Diclofenac on Steroid Glucuronidation In Vivo Do Not Affect Hair-Based Doping Tests for Stanozolol

Department of Anatomy, Histology and Embryology, Semmelweis University, Tűzoltó u. 58, Budapest 1094, Hungary
School of Life Sciences, Pharmacy and Chemistry, Kingston University, Kingston upon Thames, London KT1 2EE, UK
Department of Chemistry, College of Science, United Arab Emirates University, Al Ain 009713, UAE
Author to whom correspondence should be addressed.
Received: 23 May 2017 / Accepted: 9 June 2017 / Published: 12 June 2017
(This article belongs to the Section Metabolites)
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In vitro studies show that diclofenac inhibits enzymatic steroid glucuronidation. This study was designed to investigate the influence of diclofenac on the excretion of stanozolol and 3′-hydroxystanozolol via analyses in hair, blood and urine in vivo in a rat study. Brown Norway rats were administered with stanozolol (weeks 1–3) and diclofenac (weeks 1–6). Weekly assessment of steroid levels in hair was complemented with spot urine and serum tests. Levels of both stanozolol and 3′-hydroxystanozolol steadily increased in hair during stanozolol treatment and decreased post-treatment, but remained readily detectable for 6 weeks. In contrast, compared to control rats, diclofenac significantly reduced urinary excretion of 3′-hydroxystanozolol which was undetectable in most samples. This is the first report of diclofenac altering steroid metabolism in vivo, detrimentally affecting detection in urine, but not in hair, which holds considerable advantages over urinalysis for anti-doping tests. View Full-Text
Keywords: steroid; metabolism; anti-inflammatory drug; inhibition steroid; metabolism; anti-inflammatory drug; inhibition

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Zachár, G.; Deshmukh, N.I.; Petróczi, A.; Székely, A.D.; Shah, I.; Barker, J.; Naughton, D.P. Inhibitory Effects of Diclofenac on Steroid Glucuronidation In Vivo Do Not Affect Hair-Based Doping Tests for Stanozolol. Molecules 2017, 22, 976.

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