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Article

Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA

1
School of Chinese Medicine, Beijing Key Laboratory, Beijing University of Chinese Medicine, Beijing 100029, China
2
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
3
Beijing Institute of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
*
Author to whom correspondence should be addressed.
Hui Kong and Xueqian Wang contributed equally to this work.
Academic Editor: Derek J. McPhee
Molecules 2017, 22(6), 939; https://doi.org/10.3390/molecules22060939
Received: 5 May 2017 / Accepted: 1 June 2017 / Published: 5 June 2017
Puerarin (PUE) is a compound isolated from the roots of Pueraria lobata. We studied the pharmacokinetics and tissue distribution kinetics of PUE in Sprague-Dawley rats following intraperitoneal administration of three concentrations. Indirect competitive ELISA based on an anti-PUE monoclonal antibody was used to determine the concentration of PUE in the blood, heart, liver, spleen, lung, kidney, hippocampus, cerebral cortex, and striatum. The plasma and tissue distribution kinetic characteristics following a single injection of PUE (20, 40 and 80 mg/kg) were calculated using a non-compartment model. In the high-dose (80 mg/kg) and medium-dose (40 mg/kg) groups, the kinetic profile of PUE in blood and kidney samples showed two absorption peaks, while that of the other tissues showed only one peak. In the low-dose (20 mg/kg) group, there was only one peak, irrespective of the sample type. Pharmacokinetic parameters, such as the area under the curve, Cmax, and Tmax varied according to the administered dose. AUC and Cmax values increased dose-dependently. PUE was widely distributed in areas of the brain such as the hippocampus, cerebral cortex, and striatum, providing a foundation for guiding the use of PUE in the treatment of cerebral ischaemic stroke and neurodegenerative diseases. View Full-Text
Keywords: pharmacokinetics; tissue distribution; indirect competitive enzyme-linked immunosorbent assay; puerarin pharmacokinetics; tissue distribution; indirect competitive enzyme-linked immunosorbent assay; puerarin
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MDPI and ACS Style

Kong, H.; Wang, X.; Shi, R.; Zhao, Y.; Cheng, J.; Yan, X.; Liu, X.; Wang, Y.; Zhang, M.; Wang, Q.; Qu, H. Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA. Molecules 2017, 22, 939. https://doi.org/10.3390/molecules22060939

AMA Style

Kong H, Wang X, Shi R, Zhao Y, Cheng J, Yan X, Liu X, Wang Y, Zhang M, Wang Q, Qu H. Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA. Molecules. 2017; 22(6):939. https://doi.org/10.3390/molecules22060939

Chicago/Turabian Style

Kong, Hui, Xueqian Wang, Rongfeng Shi, Yan Zhao, Jinjun Cheng, Xin Yan, Xiaoman Liu, Yongzhi Wang, Meiling Zhang, Qingguo Wang, and Huihua Qu. 2017. "Pharmacokinetics and Tissue Distribution Kinetics of Puerarin in Rats Using Indirect Competitive ELISA" Molecules 22, no. 6: 939. https://doi.org/10.3390/molecules22060939

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