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Open AccessArticle

Endophytic Bacteria Isolated from Panax ginseng Improves Ginsenoside Accumulation in Adventitious Ginseng Root Culture

Key Laboratory of Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, Yanbian University, Yanji 133002, China
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Academic Editors: Anusha Chaparala, Lorne Hofseth and Woo-Sik Jeong
Molecules 2017, 22(6), 837; https://doi.org/10.3390/molecules22060837
Received: 7 April 2017 / Revised: 17 May 2017 / Accepted: 17 May 2017 / Published: 23 May 2017
(This article belongs to the Special Issue Current Trends in Ginseng Research)
Ginsenoside is the most important secondary metabolite of ginseng. Natural sources of wild ginseng have been overexploited. Although root culture could reduce the length of the growth cycle of ginseng, the number of ginsenosides is fewer and their contents are lower in adventitious roots of ginseng than that in ginseng cultivated in the field. In this study, we investigated the effects of endophytic bacterial elicitors on biomass and ginsenoside production in adventitious roots cultures of Panax ginseng. Endophyte LB 5-3 as an elicitor could increase biomass and ginsenoside accumulation in ginseng adventitious root culture. After 6 days elicitation with a 10.0 mL of strain LB 5-3, the content of total ginsenoside was 2.026 mg g−1 which was four times more than that in unchallenged roots. The combination of methyl jasmonate and strain LB 5-3 had a negative effect on ginseng adventitious root growth and ginsenoside production. The genomic DNA of strain LB 5-3 was sequenced, and was found to be most closely related to Bacillus altitudinis (KX230132.1). The challenged ginseng adventitious root extracts exerted inhibitory effect against the HepG2 cells, which IC50 value was 0.94 mg mL−1. View Full-Text
Keywords: adventitious root culture; antitumor; endophytic bacterial elicitor; ginsenosides; Panax ginseng adventitious root culture; antitumor; endophytic bacterial elicitor; ginsenosides; Panax ginseng
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Song, X.; Wu, H.; Yin, Z.; Lian, M.; Yin, C. Endophytic Bacteria Isolated from Panax ginseng Improves Ginsenoside Accumulation in Adventitious Ginseng Root Culture. Molecules 2017, 22, 837.

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