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Molecules 2017, 22(5), 805;

Identification and Validation of SAA4 as a Rheumatoid Arthritis Prescreening Marker by Liquid Chromatography Tandem-mass Spectrometry

Laboratory of Signal Transduction and Disease Biomarker Discovery, Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, Daejeon 34824, Korea
Research Institute of DongDeok Pharmaceutical, Chungcheongbuk-do 27864, Korea
Department of Biomedical Laboratory Science, College of Health Sciences, Eulji University, Seongnam-si, Gyeonggi-do 13135, Korea
Department of Laboratory Medicine, Korea Cancer Center Hospital, Seoul 01812, Korea
Integrative Research Support Center, College of Medicine, The Catholic University of Korea, Seoul 06591, Korea
Forensic Science R&D Lab., Police Science Institute, Chungcheongnam-do 31539, Korea
These authors contributed equally.
Authors to whom correspondence should be addressed.
Academic Editor: Gyorgy M. Keseru
Received: 4 March 2017 / Revised: 8 May 2017 / Accepted: 11 May 2017 / Published: 14 May 2017
(This article belongs to the Section Medicinal Chemistry)
Full-Text   |   PDF [3492 KB, uploaded 15 May 2017]   |  


Rheumatoid arthritis (RA) is a chronic autoimmune disease that progresses into systemic inflammation and joint deformity. RA diagnosis is a complicated procedure, and early diagnostic methods are insufficient. Therefore, in this study, we attempted to identify new markers to improve the accuracy of RA prescreening. e identified differentially expressed proteins (DEPs) by using liquid chromatography tandem-mass spectrometry in health-prescreening sera with high rheumatoid factor (RF) values, and compared the findings with those from sera with normal RF values. We identified 93 DEPs; of these, 36 were upregulated, and 57 were downregulated in high-RF sera. Pathway analysis revealed that these DEPs were related to immune responses. Additionally, four DEPs were statistically analyzed by proteomic analysis; of these, SAA4 was significantly validated in individual enzyme-linked immunosorbent assays. Moreover, SAA4 was significantly upregulated in RA patients (n = 40, 66.43 ± 12.97 ng/mL) compared with normal controls (n = 40, 4.79 ± 0.95 ng/mL) and had a higher area under the curve than C-reactive protein. Thus, we identified SAA4 as a protein that was positively correlated with RF and RA. SAA4 may represent a novel prescreening marker for the diagnosis of RA. View Full-Text
Keywords: rheumatoid factor; LC-MS/MS; pre-screening; serum amyloid A4 rheumatoid factor; LC-MS/MS; pre-screening; serum amyloid A4

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Seok, A.; Lee, H.-J.; Lee, S.; Lee, J.; Mun, S.; Park, A.; Chun, Y.-T.; Lee, J.-H.; Lim, H.-J.; Kang, H.-G. Identification and Validation of SAA4 as a Rheumatoid Arthritis Prescreening Marker by Liquid Chromatography Tandem-mass Spectrometry. Molecules 2017, 22, 805.

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