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Molecules 2017, 22(4), 660;

Evaluation of Tanshinone IIA Developmental Toxicity in Zebrafish Embryos

School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong, China
School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong, China
School of Nursing, Guangdong Pharmaceutical University, Guangzhou 510310, Guangdong, China
Chn-Alternative Biotech Co. Ltd., Guangzhou 510432, Guangdong, China
Authors to whom correspondence should be addressed.
Academic Editor: Julio A. Seijas Vázquez
Received: 25 February 2017 / Revised: 9 April 2017 / Accepted: 15 April 2017 / Published: 21 April 2017
(This article belongs to the Special Issue ECSOC-20)
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Tanshinone IIA (Tan-IIA) is derived from the dried roots of Salvia miltiorrhiza Bunge, a traditional Chinese medicine. Although Salvia miltiorrhiza has been applied for many years, the toxicity of the mono-constituent of Salvia miltiorrhiza, tanshinone IIA, is still understudied. This study evaluated the cardiotoxicity and developmental malformations of Tan-IIA by using zebrafish normal embryos and dechorionated embryos. After treatment with Tan-IIA in different concentrations for four-day periods, obvious pericardial edema, spinal curvature, and even missing tails were observed in zebrafish embryos. The LC50 values in the dechorionated embryo group at 72 h post-fertilization (hpf) and 96 hpf were 18.5 μM and 12.8 μM, respectively, and the teratogenicity was manifested at a concentration of about 1 µM. The main endpoints of teratogenicity were scoliosis, malformation of tail, and pericardium edema. Our findings displayed the potential cardiotoxicity and severe impact on the abnormal development of Tan-IIA in zebrafish embryo at high concentrations, which may help avoid the risk of its clinical application. View Full-Text
Keywords: zebrafish; tanshinone IIA; developmental toxicity zebrafish; tanshinone IIA; developmental toxicity

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Wang, T.; Wang, C.; Wu, Q.; Zheng, K.; Chen, J.; Lan, Y.; Qin, Y.; Mei, W.; Wang, B. Evaluation of Tanshinone IIA Developmental Toxicity in Zebrafish Embryos. Molecules 2017, 22, 660.

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