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Molecules 2017, 22(4), 584;

Screening and Analysis of the Marker Components in Ganoderma lucidum by HPLC and HPLC-MSn with the Aid of Chemometrics

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 3 January 2017 / Revised: 26 March 2017 / Accepted: 1 April 2017 / Published: 6 April 2017
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Ganoderma triterpenes (GTs) are the major secondary metabolites of Ganoderma lucidum, which is a popularly used traditional Chinese medicine for complementary cancer therapy. The present study was to establish a fingerprint evaluation system based on Similarity Analysis (SA), Cluster Analysis (CA) and Principal Component Analysis (PCA) for the identification and quality control of G. lucidum. Fifteen samples from the Chinese provinces of Hainan, Neimeng, Shangdong, Jilin, Anhui, Henan, Yunnan, Guangxi and Fujian were analyzed by HPLC-PAD and HPLC-MSn. Forty-seven compounds were detected by HPLC, of which forty-two compounds were tentatively identified by comparing their retention times and mass spectrometry data with that of reference compounds and reviewing the literature. Ganoderic acid B, 3,7,15-trihydroxy-11,23-dioxolanost-8,16-dien-26-oic acid, lucidenic acid A, ganoderic acid G, and 3,7-oxo-12-acetylganoderic acid DM were deemed to be the marker compounds to distinguish the samples with different quality according to both CA and PCA. This study provides helpful chemical information for further research on the anti-tumor activity and mechanism of action of G. lucidum. The results proved that fingerprints combined with chemometrics are a simple, rapid and effective method for the quality control of G. lucidum. View Full-Text
Keywords: Ganoderma lucidum; triterpenes; HPLC-MSn; Similarity Analysis (SA); chemometrics Ganoderma lucidum; triterpenes; HPLC-MSn; Similarity Analysis (SA); chemometrics

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Wu, L.; Liang, W.; Chen, W.; Li, S.; Cui, Y.; Qi, Q.; Zhang, L. Screening and Analysis of the Marker Components in Ganoderma lucidum by HPLC and HPLC-MSn with the Aid of Chemometrics. Molecules 2017, 22, 584.

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