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Molecules 2017, 22(10), 1597;

Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase

School of Port and Transportation Engineering, Zhejiang Ocean University, Zhoushan 316022, China
School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300130, China
YinzhouKefeng New Material of Polymer Co. Ltd., Ningbo 315100, China
Author to whom correspondence should be addressed.
Received: 23 August 2017 / Revised: 18 September 2017 / Accepted: 19 September 2017 / Published: 25 September 2017
(This article belongs to the Special Issue Mesoporous Silica in Biomedical Applications)
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Tannic acid-templated mesoporous silica (TAMS) was synthesized using a simple nonsurfactant template method and dopamine-functionalized TAMS (Dop-TAMS), which was prepared via a biomimetic coating, was developed as a new support for immobilization of NHase (NHase@Dop-TAMS). The Dop-TAMS was thoroughly characterized by the transmission electron microscopy (TEM), scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET), and Fourier transform infrared (FT-IR) and the results showed that the Dop-TAMS possessed sufficiently large pore size and volume for the accommodation of NHase. Studying the thermal stability, storage, shaking stability, and pH stability of the free and immobilized NHase indicated that the catalytic properties of NHase@Dop-TAMS were significantly enhanced. Moreover, the NHase@Dop-TAMS exhibited good reusability. All the results demonstrated that Dop-TAMS could be used as an excellent matrix for the immobilization of NHase. View Full-Text
Keywords: dopamine; mesoporous silica; tannic acid; immobilization; nitrile hydratase dopamine; mesoporous silica; tannic acid; immobilization; nitrile hydratase

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Gao, J.-K.; Zhang, Z.-J.; Jiang, Y.-J.; Chen, Y.; Gao, S.-F. Biomimetic-Functionalized, Tannic Acid-Templated Mesoporous Silica as a New Support for Immobilization of NHase. Molecules 2017, 22, 1597.

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