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Molecules 2016, 21(8), 1062;

Influence of Cysteine and Tryptophan Substitution on DNA-Binding Activity on Maize α-Hairpinin Antimicrobial Peptide

Programa de Pós Graduação em Patologia Molecular, Universidade de Brasília, Brasília, DF 70910-900, Brazil
Centro de Análises Proteômicas e Bioquímicas, Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF 70790-160, Brazil
S-Inova Biotech, Programa de Pós-Graduação em Biotecnologia, Universidade Catolica Dom Bosco, Campo Grande, MS 79117-900, Brazil
Author to whom correspondence should be addressed.
Academic Editor: Wade A. Russu
Received: 8 June 2016 / Revised: 29 July 2016 / Accepted: 9 August 2016 / Published: 13 August 2016
(This article belongs to the Special Issue Peptides as Candidate Chemotherapeutic Drugs)
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For almost four decades, antimicrobial peptides have been studied, and new classes are being discovered. However, for therapeutic use of these molecules, issues related to the mechanism of action must be answered. In this work, the antimicrobial activity of the hairpinin MBP-1 was studied by the synthesis of two variants, one replacing cysteines and one tryptophan with alanine. Antibacterial activity was abolished in both variants. No membrane disturbance, even in concentrations higher than those required to inhibit the bacteria, was observed in SEM microscopy. The gel retardation assay showed that MBP-1 possesses a higher DNA-binding ability than variants. Finally, molecular modelling showed that the lack of cysteines resulted in structure destabilization and lack of tryptophan resulted in a less flexible peptide, with less solvent assessable surface area, both characteristics that could contribute to absence of activity. In summary, the data here reported add more information about the multiple mechanisms of action of α-hairpinins. View Full-Text
Keywords: α-hairpinin; DNA-binding; antimicrobial peptides α-hairpinin; DNA-binding; antimicrobial peptides

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Sousa, D.A.; Porto, W.F.; Silva, M.Z.; da Silva, T.R.; Franco, O.L. Influence of Cysteine and Tryptophan Substitution on DNA-Binding Activity on Maize α-Hairpinin Antimicrobial Peptide. Molecules 2016, 21, 1062.

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