Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian
Research and Development Center of Biorational Pesticides, Northwest A & F University, Yangling 712100, China
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Author to whom correspondence should be addressed.
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These authors contributed equally to this work.
Academic Editor: Derek J. McPhee
Molecules 2016, 21(6), 756; https://doi.org/10.3390/molecules21060756
Received: 21 April 2016
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Revised: 3 June 2016
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Accepted: 6 June 2016
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Published: 11 June 2016
(This article belongs to the Section Natural Products Chemistry)
Phytophthora blight of pepper caused by Phytophthora capsici Leonian is a destructive disease throughout the world. Cuminic acid, extracted from the seed of Cuminum cyminum L., belongs to the benzoic acid chemical class. In this study, the sensitivity and biochemical response of P. capsici to cuminic acid was determined. The mean EC50 (50% effective concentration) values for cuminic acid in inhibiting mycelial growth and zoospore germination of the 54 studied P. capsici isolates were 14.54 ± 5.23 μg/mL and 6.97 ± 2.82 μg/mL, respectively. After treatment with cuminic acid, mycelial morphology, sporangium formation and mycelial respiration were significantly influenced; cell membrane permeability and DNA content increased markedly, but pyruvic acid content, adenosine triphosphate (ATP) content, and ATPase activity decreased compared with the untreated control. In pot experiments, cuminic acid exhibited both protective and curative activity. Importantly, POD and PAL activity of the pepper leaves increased after being treated with cuminic acid. These indicated that cuminic acid not only showed antifungal activity, but also could improve the defense capacity of the plants. All the results suggested that cuminic acid exhibits the potential to be developed as a new phytochemical fungicide, and this information increases our understanding of the mechanism of action of cuminic acid against Phytophthora capsici.
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Keywords:
antifungal activity; cuminic acid; Phytophthora capsici; biochemical response; defense capacity
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MDPI and ACS Style
Wang, Y.; Sun, Y.; Zhang, Y.; Zhang, X.; Feng, J. Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian. Molecules 2016, 21, 756. https://doi.org/10.3390/molecules21060756
AMA Style
Wang Y, Sun Y, Zhang Y, Zhang X, Feng J. Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian. Molecules. 2016; 21(6):756. https://doi.org/10.3390/molecules21060756
Chicago/Turabian StyleWang, Yong, Yang Sun, Ying Zhang, Xing Zhang, and Juntao Feng. 2016. "Antifungal Activity and Biochemical Response of Cuminic Acid against Phytophthora capsici Leonian" Molecules 21, no. 6: 756. https://doi.org/10.3390/molecules21060756
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