Next Article in Journal
An Approach to Characterizing the Complicated Sequential Metabolism of Salidroside in Rats
Previous Article in Journal
Inhalable Antitubercular Therapy Mediated by Locust Bean Gum Microparticles
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Molecules 2016, 21(6), 700;

Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography

College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Korea
Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702-1202, USA
These authors contributed equally to this paper.
Author to whom correspondence should be addressed.
Academic Editor: Thomas J. Schmidt
Received: 1 April 2016 / Revised: 20 May 2016 / Accepted: 24 May 2016 / Published: 28 May 2016
(This article belongs to the Section Natural Products Chemistry)
Full-Text   |   PDF [3914 KB, uploaded 28 May 2016]   |  


A simple and rapid method using high-speed counter-current chromatography (HSCCC), along with bioassay-guided fractionation based on the anti-proliferative activity against renal and colon cancer cells, has been developed for the preparative separation of aceroside VIII (1) and platyphylloside (2) from Betula platyphylla. A solvent system composed of ethyl acetate/acetonitrile/water (1:0.1:1, v/v/v) was optimized for the separation. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase. Among these isolated diarylheptanoids, platyphylloside (2) showed anti-proliferative activity in the COLO205 and KM12 colon cells and renal cancer cell lines A498, U031, as well as in MG63 and MG 63.3 osteosarcoma cells. In addition, it showed dose dependent inhibitory effects in the NCI 60 cell line assay. These results suggest that the diarylheptanoids isolated from B. platyphylla with an efficient HSCCC method could be potential multi-targeted therapeutic agents for cancer. View Full-Text
Keywords: HSCCC; diarylheptanoid; anti-proliferation HSCCC; diarylheptanoid; anti-proliferation

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Cho, N.; Kim, H.W.; Kim, T.B.; Ransom, T.T.; Beutler, J.A.; Sung, S.H. Preparative Purification of Anti-Proliferative Diarylheptanoids from Betula platyphylla by High-Speed Counter-Current Chromatography. Molecules 2016, 21, 700.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top