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Open AccessArticle

A Method for Determining the Content of Glycoproteins in Biological Samples

1
Jilin Key Laboratory of Macromolecules in Chinese Drugs, Changchun University of Chinese Traditional Medicine, Changchun 130117, Jilin, China
2
Jilin Institute For Drug Control, Changchun 130033, Jilin, China
*
Author to whom correspondence should be addressed.
Academic Editor: Quan-Bin Han
Molecules 2016, 21(12), 1625; https://doi.org/10.3390/molecules21121625
Received: 22 October 2016 / Revised: 22 November 2016 / Accepted: 22 November 2016 / Published: 26 November 2016
(This article belongs to the Special Issue Natural Polysaccharides)
The glycoprotein purified from the mycelium extract of Tremella fuciformis was marked with iodine through the iodine substitution reaction. The content of iodine, which is indicative of the amount of the marked tremella glycoprotein (ITG), was detected with Inductively coupled plasma mass spectrometry (ICP-MS). The method was found to be stable, sensitive, and accurate at detecting the content of iodine-substituted glycoprotein, and was used in the quantitative analysis of biological samples, including blood and organs. Different biological samples were collected from rats after oral administration of ITG, and were tested for iodine content by ICP-MS to calculate the amount of ITG in the samples. The results suggested that ICP-MS is a sensitive, stable, and accurate method for detection of iodinated glycoproteins in blood and organs. View Full-Text
Keywords: glycoprotein; iodine substitution; ICP-MS; biological sample; quantification glycoprotein; iodine substitution; ICP-MS; biological sample; quantification
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MDPI and ACS Style

Gao, Y.; Xu, D.; Li, H.; Yang, X.; Wang, M.; Gao, Q. A Method for Determining the Content of Glycoproteins in Biological Samples. Molecules 2016, 21, 1625.

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