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Molecules 2016, 21(1), 89;

Synthesis of Isotopically Labeled 13C3-Simazine and Development of a Simultaneous UPLC-MS/MS Method for the Analysis of Simazine in Soil

Plant Protection Institute of the Chinese Academy of Agricultural Sciences, Beijing 100193, China
College of Pharmacology, Ningxia Medical University, Yinchuan 750004, China
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 30 September 2015 / Revised: 21 December 2015 / Accepted: 6 January 2016 / Published: 14 January 2016
(This article belongs to the Section Organic Chemistry)
Full-Text   |   PDF [663 KB, uploaded 14 January 2016]   |  


The isotope dilution mass spectrometry (IDMS) is a highly efficient method for tackling the ion suppression in complex matrix by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), but a lack of commercial internal standards is a limiting factor for these analyses. Herein, an economical and efficient strategy for the synthesis of 13C3-simazine via a three-step procedure was developed. The isotope-labeled internal standard was used for determination of simazine residue in soil samples. The quantitation method has a limit of detection of 0.015 μg/kg and quantitation of 0.08 μg/kg. The inter-day and intra-day precision of the method were below 4.6%. Recovery values were ranged between 92.9% and 99.2%. All the samples obtained from six provinces in China contained from 1 to 62 μg/kg of simazine. View Full-Text
Keywords: 13C3-simazine; synthesis; IDMS; UPLC-MS/MS; isotope labelling 13C3-simazine; synthesis; IDMS; UPLC-MS/MS; isotope labelling

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Song, Y.; Guo, Y.; Zhang, X.; Yang, Y.; Chen, S.; She, G.; She, D. Synthesis of Isotopically Labeled 13C3-Simazine and Development of a Simultaneous UPLC-MS/MS Method for the Analysis of Simazine in Soil. Molecules 2016, 21, 89.

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