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Open AccessArticle

New Fluorescence Probes for Biomolecules

Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland
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Academic Editor: Derek J. McPhee
Molecules 2015, 20(7), 13071-13079; https://doi.org/10.3390/molecules200713071
Received: 12 June 2015 / Revised: 8 July 2015 / Accepted: 9 July 2015 / Published: 20 July 2015
(This article belongs to the Section Molecular Diversity)
Steady state fluorescence measurements have been used for the investigation of interaction between the bovine serum albumin (BSA) and fluorescence probes: 3-hydroxy-2,4- bis[(3-methyl-1,3-benzoxazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ6), 3-hydroxy- 2,4-bis[(3-methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ7) and 3-hydroxy-2,4-bis[(1,3,3-trimethyl-1,3-dihydro-2H-indol-2-ylidene)methyl]cyclobut-2-en-1-one (SQ8). The binding constant between bovine serum albumin and squarine dyes has been determined by using both the Benesi-Hildebrand and Stern-Volmer equations. The negative value of free energy change indicates the existence of a spontaneous complexation process of BSA with squarine dyes. View Full-Text
Keywords: bovine serum albumin; squarine dyes; fluorescence probe; biomolecule determination; fluorescence method bovine serum albumin; squarine dyes; fluorescence probe; biomolecule determination; fluorescence method
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MDPI and ACS Style

Jurek, K.; Kabatc, J.; Kostrzewska, K.; Grabowska, M. New Fluorescence Probes for Biomolecules. Molecules 2015, 20, 13071-13079.

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