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Molecules 2015, 20(7), 13071-13079;

New Fluorescence Probes for Biomolecules

Faculty of Chemical Technology and Engineering, University of Science and Technology, UTP, Bydgoszcz 85-326, Poland
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 12 June 2015 / Revised: 8 July 2015 / Accepted: 9 July 2015 / Published: 20 July 2015
(This article belongs to the Section Molecular Diversity)
Full-Text   |   PDF [1115 KB, uploaded 20 July 2015]   |  


Steady state fluorescence measurements have been used for the investigation of interaction between the bovine serum albumin (BSA) and fluorescence probes: 3-hydroxy-2,4- bis[(3-methyl-1,3-benzoxazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ6), 3-hydroxy- 2,4-bis[(3-methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]cyclobut-2-en-1-one (SQ7) and 3-hydroxy-2,4-bis[(1,3,3-trimethyl-1,3-dihydro-2H-indol-2-ylidene)methyl]cyclobut-2-en-1-one (SQ8). The binding constant between bovine serum albumin and squarine dyes has been determined by using both the Benesi-Hildebrand and Stern-Volmer equations. The negative value of free energy change indicates the existence of a spontaneous complexation process of BSA with squarine dyes. View Full-Text
Keywords: bovine serum albumin; squarine dyes; fluorescence probe; biomolecule determination; fluorescence method bovine serum albumin; squarine dyes; fluorescence probe; biomolecule determination; fluorescence method

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Jurek, K.; Kabatc, J.; Kostrzewska, K.; Grabowska, M. New Fluorescence Probes for Biomolecules. Molecules 2015, 20, 13071-13079.

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