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Open AccessArticle

Fast Simultaneous Determination of 13 Nucleosides and Nucleobases in Cordyceps sinensis by UHPLC–ESI–MS/MS

by Shi-Yu Zong 1,2,†, Han Han 2,†, Bing Wang 1,2,*, Ning Li 3, Tina Ting-Xia Dong 3, Tong Zhang 1,2,* and Karl W. K. Tsim 3
1
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, No. 1200 Cailun Road, Pudong New District, Shanghai 201203, China
2
Experimental Center of Teaching and Learning, Shanghai University of Traditional Chinese Medicine, No. 1200 Cailun Road, Pudong New District, Shanghai 201203, China
3
Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay Road, Hong Kong, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Derek J. McPhee
Molecules 2015, 20(12), 21816-21825; https://doi.org/10.3390/molecules201219807
Received: 4 November 2015 / Revised: 29 November 2015 / Accepted: 1 December 2015 / Published: 4 December 2015
(This article belongs to the Section Natural Products Chemistry)
A reliable ultra-high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (UHPLC–ESI–MS/MS) method for the fast simultaneous determination of 13 nucleosides and nucleobases in Cordyceps sinensis (C. sinensis) with 2-chloroadenosine as internal standard was developed and validated. Samples were ultrasonically extracted in an ice bath thrice, and the optimum analyte separation was performed on an ACQUITY UPLCTM HSS C18 column (100 mm × 2.1 mm, 1.8 μm) with gradient elution. All targeted analytes were separated in 5.5 min. Furthermore, all calibration curves showed good linear regression (r > 0.9970) within the test ranges, and the limits of quantitation and detection of the 13 analytes were less than 150 and 75 ng/mL, respectively. The relative standard deviations (RSDs) of intra- and inter-day precisions were <6.23%. Recoveries of the quantified analytes ranged within 85.3%–117.3%, with RSD < 6.18%. The developed UHPLC–ESI–MS/MS method was successfully applied to determine nucleosides and nucleobases in 11 batches of C. sinensis samples from different regions in China. The range for the total content in the analyzed samples was 1329–2057 µg/g. View Full-Text
Keywords: Cordyceps sinensis; nucleosides; nucleobases; UHPLC–ESI–MS/MS Cordyceps sinensis; nucleosides; nucleobases; UHPLC–ESI–MS/MS
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Zong, S.-Y.; Han, H.; Wang, B.; Li, N.; Dong, T.T.-X.; Zhang, T.; Tsim, K.W.K. Fast Simultaneous Determination of 13 Nucleosides and Nucleobases in Cordyceps sinensis by UHPLC–ESI–MS/MS. Molecules 2015, 20, 21816-21825.

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