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Enrichment and Purification of Polyphenol Extract from Sphallerocarpus gracilis Stems and Leaves and in Vitro Evaluation of DNA Damage-Protective Activity and Inhibitory Effects of α-Amylase and α-Glucosidase

1
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China
2
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
3
National HACCP Center, Chinese Academy of Inspection and Quarantine, Beijing 100123, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Isabel C. F. R. Ferreira and Nancy D. Turner
Molecules 2015, 20(12), 21442-21457; https://doi.org/10.3390/molecules201219780
Received: 6 November 2015 / Revised: 24 November 2015 / Accepted: 26 November 2015 / Published: 2 December 2015
(This article belongs to the Section Natural Products Chemistry)
An efficient preparative separation method for Sphallerocarpus gracilis stems and leaves polyphenols (SGslP) was established in this study. An X-5 macroporous adsorption resin was selected for the purification of the SGslP, and the polyphenol content of the purified SGslP (PSGslP) was increased 5.11-fold from 8.29% to 42.38% after one treatment run. The chemical composition of the PSGslP was analyzed by HPLC-MS/MS, and the predominant compounds were found to be luteolin-7-glucoside, acacetin-7-acetyglycoside and its isomers. In addition, the PSGslP was evaluated in vitro to determine the DNA damage-protective activity and inhibitory effects of α-amylase and α-glucosidase. The results indicated that the PSGslP exhibited significant protective activities against both ROO• and •OH radical-induced DNA damage. Moreover, the PSGslP exerted a dose-dependent inhibition effect on α-glucosidase but no inhibitory effect on α-amylase. These findings indicate that the Sphallerocarpus gracilis stems and leaves are good natural sources of antioxidants and are potent inhibitors of α-glucosidase activity and are potential anti-diabetic inhibitor. View Full-Text
Keywords: SGslP; purification; phenolic composition; oxidative DNA damage; enzyme inhibition SGslP; purification; phenolic composition; oxidative DNA damage; enzyme inhibition
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Ma, T.; Sun, X.; Tian, C.; Luo, J.; Zheng, C.; Zhan, J. Enrichment and Purification of Polyphenol Extract from Sphallerocarpus gracilis Stems and Leaves and in Vitro Evaluation of DNA Damage-Protective Activity and Inhibitory Effects of α-Amylase and α-Glucosidase. Molecules 2015, 20, 21442-21457.

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