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Molecules 2015, 20(10), 17848-17859;

Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens

College of Pharmacy, Qingdao University, Qingdao 266021, Shandong, China
Health Education Institute of Changchun, Changchun 130021, Jilin, China
Institute of Changbai Mountain Resources, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, Jilin, China
Author to whom correspondence should be addressed.
Academic Editor: Isabel C. F. R. Ferreira
Received: 27 August 2015 / Revised: 15 September 2015 / Accepted: 22 September 2015 / Published: 25 September 2015
(This article belongs to the Collection Bioactive Compounds)
Full-Text   |   PDF [984 KB, uploaded 25 September 2015]   |  


Activity-directed fractionation and purification processes were employed to identify xanthine oxidase (XO) inhibitory compounds from the leaves of Perilla frutescens. The total extract was evaluated in vitro on XO inhibitory activity and in vivo in an experimental model with potassium oxonate-induced hyperuricemia in mice which was used to evaluate anti-hyperuricemic activity. The crude extract showed expressive urate-lowering activity results. Solvent partitioning of the total extract followed by macroporous resin column chromatography of the n-butanol extract yielded four extracts and eluted parts. Among them, only the 70% ethanol eluted part of the n-butanol extract showed strong activity and therefore was subjected to separation and purification using various chromatographic techniques. Five compounds showing potent activity were identified by comparing their spectral data with literature values to be caffeic acid, vinyl caffeate, rosmarinic acid, methyl rosmarinate, and apigenin. These results indicate that pending further study, these compounds could be used as novel natural product agents for the treatment of hyperuricemia. View Full-Text
Keywords: Perilla frutescens (L.) Britt.; hyperuricemia; xanthine oxidase; polyphenols Perilla frutescens (L.) Britt.; hyperuricemia; xanthine oxidase; polyphenols

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Huo, L.-N.; Wang, W.; Zhang, C.-Y.; Shi, H.-B.; Liu, Y.; Liu, X.-H.; Guo, B.-H.; Zhao, D.-M.; Gao, H. Bioassay-Guided Isolation and Identification of Xanthine Oxidase Inhibitory Constituents from the Leaves of Perilla frutescens. Molecules 2015, 20, 17848-17859.

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