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Open AccessArticle

Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds

Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea
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Molecules 2014, 19(7), 10309-10319; https://doi.org/10.3390/molecules190710309
Received: 22 May 2014 / Revised: 30 June 2014 / Accepted: 4 July 2014 / Published: 16 July 2014
(This article belongs to the Section Natural Products Chemistry)
A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and β-sitosterol (4). This method was fully validated in terms of linearity (r2 > 0.999), accuracy (98.5%–100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 14 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC50 value of 3.89 ± 0.17 µM. View Full-Text
Keywords: Phaseolus angularis seeds; TLR4; HPLC-UV; validation Phaseolus angularis seeds; TLR4; HPLC-UV; validation
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MDPI and ACS Style

Hwang, J.T.; Oh, H.-M.; Kim, M.-H.; Jeong, H.J.; Lee, C.; Jang, H.-J.; Lee, S.W.; Park, C.S.; Rho, M.-C. Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds. Molecules 2014, 19, 10309-10319. https://doi.org/10.3390/molecules190710309

AMA Style

Hwang JT, Oh H-M, Kim M-H, Jeong HJ, Lee C, Jang H-J, Lee SW, Park CS, Rho M-C. Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds. Molecules. 2014; 19(7):10309-10319. https://doi.org/10.3390/molecules190710309

Chicago/Turabian Style

Hwang, Joo T.; Oh, Hyun-Mee; Kim, Mi-Hwa; Jeong, Hyung J.; Lee, Chul; Jang, Hyun-Jae; Lee, Seung W.; Park, Chan S.; Rho, Mun-Chual. 2014. "Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds" Molecules 19, no. 7: 10309-10319. https://doi.org/10.3390/molecules190710309

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