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Molecules 2014, 19(7), 10309-10319;

Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds

Eco-friendly Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si 580-185, Korea
Author to whom correspondence should be addressed.
Received: 22 May 2014 / Revised: 30 June 2014 / Accepted: 4 July 2014 / Published: 16 July 2014
(This article belongs to the Section Natural Products Chemistry)
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A reproducible analytical method using reverse-phase high liquid performance chromatography combined with UV detecting was developed for the quantitative determination of four compounds isolated from the ethanol extract of Phaseolus angularis seeds (PASE): oleanolic acid (1), oleanolic acid acetate (2), stigmasterol (3) and β-sitosterol (4). This method was fully validated in terms of linearity (r2 > 0.999), accuracy (98.5%–100.8%), precision (<0.92%), LOD (<0.0035 mg/mL), and LOQ (<0.0115 mg/mL). The effects of the PASE and isolated compounds 14 on TLR4 activation were tested in THP1-Blue cells. Among the tested substances, compound 2 showed potent inhibitory activity with an IC50 value of 3.89 ± 0.17 µM. View Full-Text
Keywords: Phaseolus angularis seeds; TLR4; HPLC-UV; validation Phaseolus angularis seeds; TLR4; HPLC-UV; validation

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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Hwang, J.T.; Oh, H.-M.; Kim, M.-H.; Jeong, H.J.; Lee, C.; Jang, H.-J.; Lee, S.W.; Park, C.S.; Rho, M.-C. Simultaneous Quantitation and Validation of Triterpenoids and Phytosteroids in Phaseolus angularis Seeds. Molecules 2014, 19, 10309-10319.

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