Regulation of Plant Immunity through Modulation of Phytoalexin Synthesis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Expression of Non-Native Stilbenoid Phytoalexins in Soybean Hairy Roots
Construct | Line | Percent Root Necrosis1 | |
---|---|---|---|
None | Control | 83.7 | a |
CsVMV/AhRS3 | 32 | 42.8 | b |
CsVMV/G4DT | 98 | 40.5 | bc |
CsVMV/G4DT | 83 | 39.0 | bc |
CsVMV/AhRS3 | 48 | 18.6 | cd |
CsVMV/AhRS3& CsVMV/ROMT | 22 | 6.7 | de |
CsVMV/AhRS3& CsVMV/ROMT | 45 | 6.5 | de |
CsVMV/AhRS3& CsVMV/ROMT | 14 | 2.8 | e |
Actin/AhRS3& CsVMV/ROMT | 67 | 1.8 | de |
Actin/AhRS3& CsVMV/ROMT | 56 | 0.0 | e |
2.2. Genetic Modulation of the Native Phytoalexin Glyceollin Synthesis in Soybean Hairy Roots
3. Experimental
3.1. Genetic Modification of Soybean Hairy Roots
3.2. Molecular Analysis
3.3. Analysis of Phenylpropanoids in Soybean Tissue
3.4. Evaluation of Fungal Colonization of Hairy Root Cultures
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zernova, O.V.; Lygin, A.V.; Pawlowski, M.L.; Hill, C.B.; Hartman, G.L.; Widholm, J.M.; Lozovaya, V.V. Regulation of Plant Immunity through Modulation of Phytoalexin Synthesis. Molecules 2014, 19, 7480-7496. https://doi.org/10.3390/molecules19067480
Zernova OV, Lygin AV, Pawlowski ML, Hill CB, Hartman GL, Widholm JM, Lozovaya VV. Regulation of Plant Immunity through Modulation of Phytoalexin Synthesis. Molecules. 2014; 19(6):7480-7496. https://doi.org/10.3390/molecules19067480
Chicago/Turabian StyleZernova, Olga V., Anatoli V. Lygin, Michelle L. Pawlowski, Curtis B. Hill, Glen L. Hartman, Jack M. Widholm, and Vera V. Lozovaya. 2014. "Regulation of Plant Immunity through Modulation of Phytoalexin Synthesis" Molecules 19, no. 6: 7480-7496. https://doi.org/10.3390/molecules19067480