Nanostructured Lipid Systems as a Strategy to Improve the in Vitro Cytotoxicity of Ruthenium(II) Compounds
Abstract
:1. Introduction
2. Results and Discussion
2.1. Nanostructured Lipid System
Formulation | Mean Diameter ± S.D. (nm) * | Mean PDI ± S.D. * |
---|---|---|
ME | 129.1 ± 0.9 | 0.152 ± 0.01 |
SCAR1 | 188.0 ± 1 | 0.211 ± 0.02 |
SCAR2 | 167.9 ± 2 | 0.211 ± 0.00 |
SCAR4 | 213.0 ± 3 | 0.258 ± 0.01 |
2.2. Biological Results of Encapsulated and Unencapsulated Ruthenium(II) Complexes
Identification | Compounds | MIC90 | IC50 | SI | ||
---|---|---|---|---|---|---|
µg/mL | µM | µg/mL | µM | |||
SCAR 1 (DMSO) | [Ru(pic)(dppb)(bipy)]PF6 | 1.6 | 1.7 | 45.6 | 47.6 | 28.0 |
SCAR2 (DMSO) | [Ru(pic)(dppb)(Me-bipy)]PF6 | 1.9 | 1.9 | 22.3 | 22.7 | 12.0 |
SCAR4 (DMSO) | [Ru(pic)(dppb)(phen)]PF6 | 1.7 | 2.0 | 32.0 | 37.6 | 18.8 |
SCAR1 (ME) | [Ru(pic)(dppb)(bipy)]PF6 | 6.1 | 6.4 | 367.6 | 383.7 | 60.0 |
SCAR2 (ME) | [Ru(pic)(dppb)(Me-bipy)]PF6 | 2.9 | 3.0 | 500 | 510.2 | 170.1 |
SCAR4 (ME) | [Ru(pic)(dppb)(phen)]PF6 | 2.9 | 3.4 | 70.5 | 82.8 | 24.4 |
3. Experimental
3.1. Inorganic Compounds
3.2. Nanostructured Lipid System Preparation
3.3. Nanostructured Lipid System Characterization: Mean diameter and Polydispersity Index (PDI)
3.4. Preparation of the Coordination Compound-Loaded Nanostructured Lipid System
3.5. Determination of Minimal Inhibitory Concentration (MIC90)
3.6. In Vitro Cytotoxic Activity
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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De Freitas, E.S.; Da Silva, P.B.; Chorilli, M.; Batista, A.A.; De Oliveira Lopes, É.; Silva, M.M.d.; Leite, C.Q.F.; Pavan, F.R. Nanostructured Lipid Systems as a Strategy to Improve the in Vitro Cytotoxicity of Ruthenium(II) Compounds. Molecules 2014, 19, 5999-6008. https://doi.org/10.3390/molecules19055999
De Freitas ES, Da Silva PB, Chorilli M, Batista AA, De Oliveira Lopes É, Silva MMd, Leite CQF, Pavan FR. Nanostructured Lipid Systems as a Strategy to Improve the in Vitro Cytotoxicity of Ruthenium(II) Compounds. Molecules. 2014; 19(5):5999-6008. https://doi.org/10.3390/molecules19055999
Chicago/Turabian StyleDe Freitas, Eduardo Sinesio, Patricia Bento Da Silva, Marlus Chorilli, Alzir Azevedo Batista, Érica De Oliveira Lopes, Monize Martins da Silva, Clarice Queico Fujimura Leite, and Fernando Rogério Pavan. 2014. "Nanostructured Lipid Systems as a Strategy to Improve the in Vitro Cytotoxicity of Ruthenium(II) Compounds" Molecules 19, no. 5: 5999-6008. https://doi.org/10.3390/molecules19055999