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Molecules 2014, 19(4), 4654-4668;

Silymarin Induces Expression of Pancreatic Nkx6.1 Transcription Factor and β-Cells Neogenesis in a Pancreatectomy Model

Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana-Xochimilco, México City 04960, Mexico
Facultad de Medicina, Universidad Nacional Autónoma de México, México City 04510, Mexico
Author to whom correspondence should be addressed.
Received: 19 December 2013 / Revised: 21 March 2014 / Accepted: 31 March 2014 / Published: 15 April 2014
(This article belongs to the Section Medicinal Chemistry)
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A physio-pathological feature of diabetes mellitus is a significant reduction of β-pancreatic cells. The growth, differentiation and function maintenance of these cells is directed by transcription factors. Nkx6.1 is a key transcription factor for the differentiation, neogenesis and maintenance of β-pancreatic cells. We reported that silymarin restores normal morphology and endocrine function of damaged pancreatic tissue after alloxan-induced diabetes mellitus in rats. The aim of this study was to analyze the effect of silymarin on Nkx6.1 transcription factor expression and its consequence in β cells neogenesis. Sixty male Wistar rats were partially pancreatectomized and divided into twelve groups. Six groups were treated with silymarin (200 mg/Kg p.o) for periods of 3, 7, 14, 21, 42 and 63 days. Additionally, an unpancreatectomized control group was used. Nkx6.1 and insulin gene expression were assessed by RT-PCR assay in total pancreatic RNA. β-Cell neogenesis was determined by immunoperoxidase assay. Silymarin treated group showed an increase of Nkx6.1 and insulin genic expression. In this group, there was an increment of β-cell neogenesis in comparison to pancreatectomized untreated group. Silymarin treatment produced a rise in serum insulin and serum glucose normalization. These results suggest that silymarin may improve the reduction of β pancreatic cells observed in diabetes mellitus. View Full-Text
Keywords: insulin; pancreatic regeneration; pancreatic transcription factors insulin; pancreatic regeneration; pancreatic transcription factors

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Soto, C.; Raya, L.; Pérez, J.; González, I.; Pérez, S. Silymarin Induces Expression of Pancreatic Nkx6.1 Transcription Factor and β-Cells Neogenesis in a Pancreatectomy Model. Molecules 2014, 19, 4654-4668.

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