Next Article in Journal
Transition Metal Complexes and Radical Anion Salts of 1,10-Phenanthroline Derivatives Annulated with a 1,2,5-Tiadiazole and 1,2,5-Tiadiazole 1,1-Dioxide Moiety: Multidimensional Crystal Structures and Various Magnetic Properties
Next Article in Special Issue
Addition of Bases to the 5'-end of Human Telomeric DNA: Influences on Thermal Stability and Energetics of Unfolding
Previous Article in Journal
Gastroprotective Mechanisms of Action of Semisynthetic Carnosic Acid Derivatives in Human Cells
Previous Article in Special Issue
G-Quadruplex Guanosine Gels and Single Walled Carbon Nanotubes
Open AccessArticle

The Human Telomere Sequence, (TTAGGG)4, in the Absence and Presence of Cosolutes: A Spectroscopic Investigation

Department of Chemistry and Biochemistry, Texas Woman's University, Denton, TX 76204, USA
*
Author to whom correspondence should be addressed.
Molecules 2014, 19(1), 595-608; https://doi.org/10.3390/molecules19010595
Received: 6 November 2013 / Revised: 13 December 2013 / Accepted: 18 December 2013 / Published: 6 January 2014
(This article belongs to the Special Issue G-Quadruplexes & i-Motif DNA)
Historically, biophysical studies of nucleic acids have been carried out under near ideal conditions, i.e., low buffer concentration (e.g., 10 mM phosphate), pH 7, low ionic strength (e.g., 100 mM) and, for optical studies, low concentrations of DNA (e.g., 1 × 10−6 M). Although valuable structural and thermodynamic data have come out of these studies, the conditions, for the most, part, are inadequate to simulate realistic cellular conditions. The increasing interest in studying biomolecules under more cellular-like conditions prompted us to investigate the effect of osmotic stress on the structural and thermodynamic properties of DNA oligomers containing the human telomere sequence (TTAGGG). Here, we report the characterization of (TTAGGG)4 in potassium phosphate buffer with increasing percent PEG (polyethylene glycol) or acetonitrile. In general, the presence of these cosolutes induces a conformational change from a unimolecular hybrid structure to a multimolecular parallel stranded structure. Hence, the structural change is accompanied with a change in the molecularity of quadruplex formation. View Full-Text
Keywords: DNA quadruplexes; circular dichroism; PEG; osmotic stress DNA quadruplexes; circular dichroism; PEG; osmotic stress
Show Figures

Graphical abstract

MDPI and ACS Style

Sharma, V.R.; Sheardy, R.D. The Human Telomere Sequence, (TTAGGG)4, in the Absence and Presence of Cosolutes: A Spectroscopic Investigation. Molecules 2014, 19, 595-608. https://doi.org/10.3390/molecules19010595

AMA Style

Sharma VR, Sheardy RD. The Human Telomere Sequence, (TTAGGG)4, in the Absence and Presence of Cosolutes: A Spectroscopic Investigation. Molecules. 2014; 19(1):595-608. https://doi.org/10.3390/molecules19010595

Chicago/Turabian Style

Sharma, Vishal R.; Sheardy, Richard D. 2014. "The Human Telomere Sequence, (TTAGGG)4, in the Absence and Presence of Cosolutes: A Spectroscopic Investigation" Molecules 19, no. 1: 595-608. https://doi.org/10.3390/molecules19010595

Find Other Styles

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Search more from Scilit
 
Search
Back to TopTop