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Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin

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Department of Otolaryngology, Chi Mei Medical Center, Liouying, Tainan 717, Taiwan
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Department of Health and Nutrition, Chia-Nan University of Pharmacy and Science, No.60, Sec. 1, Erren Rd., Rende Dist., Tainan 717, Taiwan
3
Department of Biotechnology, Chia-Nan University of Pharmacy and Science, Tainan 717, Taiwan
*
Author to whom correspondence should be addressed.
Molecules 2014, 19(1), 1258-1272; https://doi.org/10.3390/molecules19011258
Received: 11 December 2013 / Revised: 9 January 2014 / Accepted: 14 January 2014 / Published: 20 January 2014
(This article belongs to the Section Natural Products Chemistry)
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug. View Full-Text
Keywords: Polygonum cuspidatum; emodin; antiviral activity; EBV Polygonum cuspidatum; emodin; antiviral activity; EBV
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Yiu, C.-Y.; Chen, S.-Y.; Yang, T.-H.; Chang, C.-J.; Yeh, D.-B.; Chen, Y.-J.; Lin, T.-P. Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate Subfraction Separated from Polygonum cuspidatum Root and Its Major Component, Emodin. Molecules 2014, 19, 1258-1272.

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