The Effect of Camphor and Borneol on Rat Thymocyte Viability and Oxidative Stress
Abstract
:1. Introduction
2. Results and Discussion
Compound | Concentration (µg/mL) | Cytotoxicity (CCK-8 assay) |
---|---|---|
0.5 | 0.927 ± 0.132 | |
Camphor | 5 | 1.024 ± 0.083 |
50 | 1.111 ± 0.033 * | |
0.5 | 1.067 ± 0.004 ** | |
Borneol | 5 | 1.070 ± 0.015 ** |
50 | 0.975 ± 0.144 | |
Control cells | 0.999 ± 0.005 |
Compound | Concentration (µg/mL) | Intracellular ROS production |
---|---|---|
0.5 | 1.413 ± 0.068 *** | |
Camphor | 5 | 1.399 ± 0.093 ** |
50 | 1.251 ± 0.049 * | |
0.5 | 1.268 ± 0.102 | |
Borneol | 5 | 1.224 ± 0.108 |
50 | 1.164 ± 0.191 | |
Control cells | 1 ± 0.042 |
Compound | Concentration (µg/mL) | Mitochondrial membrane potential |
---|---|---|
0.5 | 0.740 ± 0.071 ** | |
Camphor | 5 | 0.919 ± 0.047 |
50 | 0.925 ± 0.132 | |
0.5 | 0.799 ± 0.022 *** | |
Borneol | 5 | 0.856 ± 0.084 |
50 | 0.876 ± 0.110 | |
Control cells | 0.999 ± 0.076 |
3. Experimental
3.1. Animals
3.2. Materials
3.3. Preparation of Thymocytes
3.4. Cell Culture
3.5. Analysis of Cell Viability
3.6. Measurement of Intracellular Reactive Oxygen Species (ROS) Production
3.7. Determination of Mitochondrial Membrane Potential
3.8. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Cherneva, E.; Pavlovic, V.; Smelcerovic, A.; Yancheva, D. The Effect of Camphor and Borneol on Rat Thymocyte Viability and Oxidative Stress. Molecules 2012, 17, 10258-10266. https://doi.org/10.3390/molecules170910258
Cherneva E, Pavlovic V, Smelcerovic A, Yancheva D. The Effect of Camphor and Borneol on Rat Thymocyte Viability and Oxidative Stress. Molecules. 2012; 17(9):10258-10266. https://doi.org/10.3390/molecules170910258
Chicago/Turabian StyleCherneva, Emiliya, Voja Pavlovic, Andrija Smelcerovic, and Denitsa Yancheva. 2012. "The Effect of Camphor and Borneol on Rat Thymocyte Viability and Oxidative Stress" Molecules 17, no. 9: 10258-10266. https://doi.org/10.3390/molecules170910258