Synthesis and Characterization of Cationic Glycidyl-Based Poly(aminoester)-Folic Acid Targeting Conjugates and Study on Gene Delivery
Abstract
:1. Introduction
2. Results and Discussion
2.1. Structural Characterizations of EPAE-FA
2.2. Size and Zeta-Potential Analysis of Polymer/DNA Complexes
2.3. Buffering Capacity of Polymers
2.4. Cytotoxicity of Polymers/DNA
2.5. DNA Gel Retardation Assay
2.6. Cellular Delivery of Plasmid DNA via EPAE-FA Vectors
3. Experimental
3.1. Materials
3.2. Polymer Characterizations
3.3. Synthesis of EPAE-FA12k (EPAE-FA14k)
3.4. Characterization of EPAE Moiety of EPAE-FA
3.5. Characterization of FA Moiety of EPAE-FA
3.6. Acid-Base Titration Assay of Polymers
3.7. XTT Assay
3.8. Formation of Polymer/DNA Complexes
3.9. Characterizations of Polymer/DNA Complexes
3.10. DNA Gel Retardation Assay of Polymer/DNA Complexes
3.11. Transfection Protocol and ONPG Assay
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Tsai, P.F.; Chang, W.Y.; Hsiao, Y.C.; Li, K.J.; Shau, M.D. Synthesis and Characterization of Cationic Glycidyl-Based Poly(aminoester)-Folic Acid Targeting Conjugates and Study on Gene Delivery. Molecules 2012, 17, 9056-9069. https://doi.org/10.3390/molecules17089056
Tsai PF, Chang WY, Hsiao YC, Li KJ, Shau MD. Synthesis and Characterization of Cationic Glycidyl-Based Poly(aminoester)-Folic Acid Targeting Conjugates and Study on Gene Delivery. Molecules. 2012; 17(8):9056-9069. https://doi.org/10.3390/molecules17089056
Chicago/Turabian StyleTsai, Pai Feng, Wei Yang Chang, Yu Che Hsiao, Kuo Jung Li, and Min Da Shau. 2012. "Synthesis and Characterization of Cationic Glycidyl-Based Poly(aminoester)-Folic Acid Targeting Conjugates and Study on Gene Delivery" Molecules 17, no. 8: 9056-9069. https://doi.org/10.3390/molecules17089056