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Open AccessArticle

The Protection of Selenium against Cadmium-Induced Cytotoxicity via the Heat Shock Protein Pathway in Chicken Splenic Lymphocytes

Department of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
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Molecules 2012, 17(12), 14565-14572; https://doi.org/10.3390/molecules171214565
Received: 9 November 2012 / Revised: 3 December 2012 / Accepted: 5 December 2012 / Published: 7 December 2012
(This article belongs to the Special Issue Selenium and Tellurium Chemistry)
Cadmium (Cd) is a heavy metal that poses a hazard to animal health due to its toxicity. Selenium (Se) is an important nutritional trace element. However, the potential protective effects of Se against Cd-induced toxicity remain to be elucidated. To investigate the cytotoxicity of Cd on bird immunocytes in vitro and the protective effects of Se against exposure to Cd, chicken splenic lymphocytes received Cd (10−6 mol/L), Se (10−7 mol/L), and the mixture of 10−7 mol/L Se and 10−6 mol/L Cd and were incubated for 12 h, 24 h, 36 h, 48 h, respectively. The transcription of heat shock protein (HSP) 27, HSP40, HSP60, HSP70 and HSP90 mRNA was tested by fluorescence quantitative PCR. The results showed that the mRNA expression of HSPs exposed to 10−6 mol/L Cd showed a sustained decrease at 12–48 h exposure. A statistically significant increase in the mRNA expression of HSPs in the case of Se group was observed, as compared to the control group of chicken splenic lymphocytes. Concomitantly, treatment of chicken splenic lymphocytes with Se in combination with Cd enhanced the mRNA expression of HSPs which were reduced by Cd treatment. This indicated that the protective effect of Se against the toxicity of Cd might, at least partially, be attributed to stimulation of the level of HSPs. View Full-Text
Keywords: cadmium; selenium; heat shock protein; chicken splenic lymphocytes; protective effects cadmium; selenium; heat shock protein; chicken splenic lymphocytes; protective effects
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Chen, X.; Zhu, Y.-H.; Cheng, X.-Y.; Zhang, Z.-W.; Xu, S.-W. The Protection of Selenium against Cadmium-Induced Cytotoxicity via the Heat Shock Protein Pathway in Chicken Splenic Lymphocytes. Molecules 2012, 17, 14565-14572.

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