Next Article in Journal
Chemically Induced Photoswitching of Fluorescent Probes—A General Concept for Super-Resolution Microscopy
Next Article in Special Issue
High Affinity, Developability and Functional Size: The Holy Grail of Combinatorial Antibody Library Generation
Previous Article in Journal
Components and Insecticidal Activity against the Maize Weevils of Zanthoxylum schinifolium Fruits and Leaves
Previous Article in Special Issue
Miniproteins as Phage Display-Scaffolds for Clinical Applications
Open AccessReview

Lambda-Display: A Powerful Tool for Antigen Discovery

Sigma-tau Industrie Farmaceutiche Riunite S.p.A., Rome, Italy
*
Author to whom correspondence should be addressed.
Molecules 2011, 16(4), 3089-3105; https://doi.org/10.3390/molecules16043089
Received: 12 January 2011 / Revised: 7 April 2011 / Accepted: 12 April 2011 / Published: 13 April 2011
(This article belongs to the Special Issue Phage Display of Combinatorial Libraries)
Since its introduction in 1985, phage display technology has been successfully used in projects aimed at deciphering biological processes and isolating molecules of practical value in several applications. Bacteriophage lambda, representing a classical molecular cloning and expression system has also been exploited for generating large combinatorial libraries of small peptides and protein domains exposed on its capsid. More recently, lambda display has been consistently and successfully employed for domain mapping, antigen discovery and protein interaction studies or, more generally, in functional genomics. We show here the results obtained by the use of large libraries of cDNA and genomic DNA for the molecular dissection of the human B-cell response against complex pathogens, including protozoan parasites, bacteria and viruses. Moreover, by reviewing the experimental work performed in recent investigations we illustrate the potential of lambda display in the diagnostics field and for identifying antigens useful as targets for vaccine development. View Full-Text
Keywords: bacteriophage lambda; phage display; antigen discovery; diagnostic assay; vaccine bacteriophage lambda; phage display; antigen discovery; diagnostic assay; vaccine
Show Figures

Graphical abstract

MDPI and ACS Style

Beghetto, E.; Gargano, N. Lambda-Display: A Powerful Tool for Antigen Discovery. Molecules 2011, 16, 3089-3105.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop