Next Article in Journal
Joint Channel Estimation and Synchronization Techniques for Time-Interleaved Block-Windowed Burst OFDM
Next Article in Special Issue
Mg,Sr-Cosubstituted Hydroxyapatite with Improved Structural Properties
Previous Article in Journal
Specific Absolute Velocity Thresholds during Male Basketball Games Using Local Positional System; Differences between Age Categories
Previous Article in Special Issue
Preliminary Study on the Development of In Vitro Human Respiratory Epithelium Using Collagen Type I Scaffold as a Potential Model for Future Tracheal Tissue Engineering
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Applied Sciences
Volume 11
Issue 10
10.3390/app11104405
applsci-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model

by
Maria Teresa Colangelo
,
Silvana Belletti
,
Paolo Govoni
,
Stefano Guizzardi
and
Carlo Galli
*
Department of Medicine and Surgery, Histology and Embryology Lab, University of Parma, Via Volturno 39, 43126 Parma, Italy
*
Author to whom correspondence should be addressed.
Appl. Sci. 2021, 11(10), 4405; https://doi.org/10.3390/app11104405
Submission received: 25 April 2021 / Revised: 8 May 2021 / Accepted: 10 May 2021 / Published: 12 May 2021
(This article belongs to the Special Issue Application of Biomaterials for Tissue Engineering)
Browse Figures
Versions Notes

Abstract

Polynucleotides (PN) have long been known as an effective supportive therapy for wound healing. The present study investigated whether a hydrogel formulation containing PN and hyaluronic acid (PN + HA) could promote wound healing in an in vitro model of gingival fibroblasts. PN promoted cell growth and viability as assessed by different assays, and PN + HA, though not significantly further increasing cell growth as compared to PN, supported the formation of dense multilayered cell nodules. PN promoted fibroblasts’ clonogenic efficiency and PN + HA further enhanced the formation of more numerous dense colonies. PN + HA appeared to significantly increase the expression of collagen 1a1 and 3a1, while not affecting proteoglycans deposition. Interestingly, when tested in a scratch assay, PN + HA achieved gap closure after 48 h, while cells in the comparison groups had not completely bridged the scratch even after 96 h. Taken together, these results demonstrate that PN + HA is a promising candidate for a supportive therapy to promote soft tissue healing in the oral cavity.
Keywords: polynucleotides; hyaluronic acid; fibroblasts; wound healing; cell viability; gingiva polynucleotides; hyaluronic acid; fibroblasts; wound healing; cell viability; gingiva

Share and Cite

MDPI and ACS Style

Colangelo, M.T.; Belletti, S.; Govoni, P.; Guizzardi, S.; Galli, C. A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model. Appl. Sci. 2021, 11, 4405. https://doi.org/10.3390/app11104405

AMA Style

Colangelo MT, Belletti S, Govoni P, Guizzardi S, Galli C. A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model. Applied Sciences. 2021; 11(10):4405. https://doi.org/10.3390/app11104405

Chicago/Turabian Style

Colangelo, Maria Teresa, Silvana Belletti, Paolo Govoni, Stefano Guizzardi, and Carlo Galli. 2021. "A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model" Applied Sciences 11, no. 10: 4405. https://doi.org/10.3390/app11104405

APA Style

Colangelo, M. T., Belletti, S., Govoni, P., Guizzardi, S., & Galli, C. (2021). A Biomimetic Polynucleotides–Hyaluronic Acid Hydrogel Promotes Wound Healing in a Primary Gingival Fibroblast Model. Applied Sciences, 11(10), 4405. https://doi.org/10.3390/app11104405

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop