Search Results (12)

Chronic atrophic gastritis and intestinal metaplasia (IM) are gastric precancerous conditions (GPCs) associated with an increased risk of gastric cancer. Early detection and accurate characterization of GPC are therefore crucial for risk stratification and the implementation of preventive strategies. In the absence of clear mucosal changes observed through white-light imaging (WLI) or virtual chromoendoscopy, endocytoscopy can help unveil the presence of GPC by enabling in vivo assessment of nuclear and cellular structures at ultra-high magnification. Endocytoscopy is typically performed using WLI following dye-based staining of the mucosa. In this case, we demonstrate that combining endocytoscopy with the texture and color enhancement imaging (TXI) mode substantially improves the assessment of the gastric mucosa. In a 61-year-old man undergoing esophagogastroduodenoscopy, WLI showed multifocal erythema in the stomach, without clearly visible lesions on either WLI or narrow-band imaging. Conventional endocytoscopy revealed multiple small spots of IM with characteristic changes in glandular structures, which were even more evident when using the TXI mode. Histological analysis of targeted biopsies confirmed small foci of IM in both the antrum and corpus. The patient was enrolled in a surveillance program because of his clinical background. The combination of endocytoscopy with the TXI mode significantly enhances the delineation of mucosal and cellular architecture, supporting a more accurate optical diagnosis. Full article

Background: Early caries management is essential to enable reversal of white spot lesions without the further need for operative interventions, especially in primary dentition. Silver-based compounds can be quite effective in arresting caries lesions; however, a major drawback is teeth staining. This study aimed to evaluate the remineralization potential and aesthetic effects of novel, green-synthesized nano-silver fluoride (NSF) on artificial white spot lesions in primary teeth in comparison to 38% silver diamine fluoride (SDF) and silver diamine fluoride/potassium iodide (SDF/KI). Materials and Methods: NSF was synthesized using green tea extract. Sixty primary teeth specimens with artificial enamel lesions were randomly divided into five groups depending on the applied material: 38% SDF, 38% SDF/KI, single and double application of NSF, and control group. Treatments were followed by pH cycling. Surface microhardness and photographic analysis were conducted to assess remineralization and staining. Statistical analysis was conducted using non-parametric tests including Kruskal–Wallis and Mann–Whitney U tests with Bonferroni correction. Results: The greatest increase in microhardness was observed in the group receiving double NSF application. Its remineralizing potential was comparable to that of 38% SDF/KI, with no statistically significant difference (p = 1.000). Importantly, NSF-treated samples exhibited no teeth discoloration, unlike the black staining observed in SDF and SDF/KI groups. Conclusions: Green-synthesized NSF is a promising alternative to conventional SDF and SDF/KI, offering remineralization benefits without compromising aesthetics. The eco-friendly formulation and non-staining properties support its potential for clinical use in pediatric dentistry. Full article

Paper and cellulose-based materials are known for their sensitivity to humidity, which can create stresses among fibres and increase fragility. More importantly, humidity can lead to the formation of mould and stains, compromising both aesthetic value and long-term preservation, particularly for historical documents and books. This study explored the application of in situ prepared Zeolitic Imidazolate Framework (ZIF-8), a zinc-based MOF, on paper as a potential antimicrobial material. Hand-made and commercially printed papers were tested to assess the effective deposition and formation of the ZIF-8 network, with a focus on both visual appearance and physicochemical characteristics. X-ray fluorescence and diffraction, infrared spectroscopy, and scanning electron microscopy analysis confirmed the successful formation of the ZIF-8 network in all papers. The Zn content varied, as expected, depending on application time and paper characteristics. All treated papers exhibited minor variations in brilliance and showed slightly increased rigidity. The formation of white spots linked to Zn accumulation was observed, particularly in printed books where colourimetric and microscopic variations were more pronounced. Full article

Backgrounds/Objectives: The aim of this study was to compare differences in the physical characteristics of lissamine green (LG) strips and the outcomes of using different staining techniques. Methods: Two separate complementary investigations were conducted. Physical study: Differences between four LG strips were evaluated in terms of material, dye concentration, and dye absorption. In vivo study: Bulbar conjunctival staining was compared for four application methods of I-DEW LG strips presented in a randomized order for twenty-two participants: (1) single application 5 s after wetting (also repeated using GreenGlo for comparison), (2) single application using two strips held together, 5 s after wetting, (3) two applications using a single LG strip 5 s after wetting, 1 minute apart, (4) the same as method 3, with a single fluorescein strip in between LG applications. White light imaging was performed immediately following application and after 30, 60, 90, and 300 s. Three masked practitioners independently evaluated the randomized staining images for spot count and staining intensity. Results: Physical study: Strip paper fibres demonstrated visible similarities, with no difference in saline absorption (p > 0.05). LG concentration increased as saline retention duration increased (F = 964.1, p < 0.001), and GreenGlo tips were significantly darker (F = 2775.2, p < 0.001). In vivo study: I-DEW application resulted in less conjunctival staining than GreenGlo (p < 0.001). Amongst I-DEW application techniques, staining levels were similar (p > 0.05); however, staining intensity was significantly higher following two applications of I-DEW, 1 min apart, compared to a single application (p = 0.042). Both spot count and staining intensity decreased with time (p < 0.001). Conclusions: Two applications of I-DEW using a single strip, 1 min apart, after wetting with a single drop of saline provided maximal staining. There was also a significant difference in staining intensity observed between LG products. Full article

This study assessed the susceptibility to staining and surface roughness of white-spot lesions (WSLs) treated with resin infiltration (RIT) and microabrasion (MA) under simulated aging through thermocycling in red wine. Seventy-eight extracted human premolars with artificial WSLs were divided into three groups: untreated WSLs (control), RIT-treated (ICON^®, DMG), and MA-treated (Opalustre^®, Ultradent). Each group was further split: one subgroup immersed in artificial saliva and the other thermocycled in red wine. The color change (∆E) and surface roughness (R_a) were measured before and after staining using a spectrophotometer and a non-contact profilometer. Thermocycling in red wine increased color change (ΔE) across all groups, with the highest values observed for MA (43.94 ± 3.57), followed by RIT (31.40 ± 4.89). Surface roughness (R_a) was highest in untreated WSLs (0.61 ± 0.18 µm) and lowest in RIT (0.15 ± 0.03 µm). While RIT and MA similarly improved WSL appearance, RIT exhibited superior smoothness. These findings suggest that RIT provides a more durable surface with reduced roughness, although staining susceptibility remains comparable to MA. Moderate positive correlation was found between ΔE and Ra, indicating that roughness is one of the factors influencing color changes. Full article

Silicon wafer slicing is a crucial process during solar cell fabrication, but it often stains the silicon wafer surface. Thus, this work systematically investigated the composition, source, and cleaning method of typical white spot stains on silicon wafer surfaces. The EDS and XPS results showed that the white spot stains contained CaCO₃ and SiO₂ that were consistent with the filler components in sticky silicon ingot glue. The effects of stains on copper deposition and copper-assisted chemical etching were studied. White spot stains remained attached to the silicon surface after deposition and etching. These stains affected the uniform deposition of copper particles on the surface of the silicon wafer and also impeded the catalytic etching of copper particles. Finally, KOH solution was combined with an ultrasonic field to remove surface stains from the silicon wafer. This study provides important guidance for the removal of silicon wafer contaminants to fabricate high-efficiency solar cells. Full article

In this study, four new species of Russula subg. Brevipedum collected from China are described based on morphological characteristics and molecular evidence. Russula brevispora has a white body whose whole parts often stain brownish-orange or grayish-orange, extremely crowded lamellae with the presence of lamellulae, basidiospores with low warts and an inamyloid suprahilar spot, and clavate or lageniform hymenial cystidia often with a papillary or submoniliform appendage. Russula flavescens is characterized by a white pileus often turning yellowish brown when touched, white lamellae turning brown or light orange, basidiospores with an inamyloid suprahilar spot, and fusiform hymenial cystidia often with an appendage. Russula longicollis is morphologically characterized by a white pileus, turning grayish orange when bruised, white lamellae turning pale yellow when bruised, basidiospores with isolated warts and an amyloid suprahilar spot, and fusiform hymenial cystidia usually with a long appendage. Russula pseudojaponica has a yellowish-brown pileus center, yellowish lamellae unchanging when bruised, basidiospores with low warts and an inamyloid suprahilar spot, clavate hymenial cystidia often with a papillary appendage, and clavate pileocystidia with granulose contents. Phylogenetic analyses showed that R. flavescens, R. brevispora, and R. pseudojaponica are members of the subsect. Pallidosporinae, whereas R. longicollis belongs to subsect. Lactarioideae, and is somewhat related to R. leucocarpa. Full article

The Alcobacenses are one of the most important Portuguese collections of Monastic codices. During a campaign study to further advance our knowledge on this important legacy and its state of conservation, a heavily deteriorated codex (ALC.338) stood out due to the presence of purple stains on the lower margins on virtually all folios. In some of them, the purple stains are accompanied by yellow and brown patches as well. Also noticed was the presence of these purple spots on the white tawed leather covering the codex. The codex was analyzed macro and microscopically, chemically and biologically using state-of-the-art technology such as next-generation sequencing. The biological deterioration associated with the appearance of these purple spots arises from the inside out and reveals significant abundance of the bacterial genus Saccharopolyspora sp., which accounted for 86% of the bacterial genera identified. For the fungal population, the most common genus was Malassezia sp. Visual inspection of the sample confirmed the presence of fungal and bacterial biofilms. Pressing questions, comparison to similar studies and future perspectives are also given. Full article

Lewy bodies (LB) play a neuropathological role in Parkinson’s disease (PD). Our goal was to evaluate LB using anti-ubiquitin immunohistochemistry (UIHC) and find correlations with monoamine oxidase-A (MAO-A) using imaging agent, [¹⁸F]FAZIN3. Human post-mortem anterior cingulate (AC) and corpus callosum (CC) from control subjects (CN), n = 6; age 81–90 LB = 0 and PD, n = 6, age 77–89, LB = III–IV were sectioned (10 μm slices). Brain slices were immunostained with anti-ubiquitin for LB (UIHC) and analyzed using QuPath for percent anti-ubiquitin per unit area (μm²). Adjacent brain slices were incubated with [¹⁸F]FAZIN3 and cortical layers I–III, IV–VI and CC (white matter) regions were quantified for the binding of [¹⁸F]FAZIN3. UIHC was correlated with [¹⁸F]FAZIN3 binding. All PD brains were positively UIHC stained and confirmed presence of LB. Outer cortical layers (I–III) of PD AC had 21% UIHC while inner layers (IV–VI) had >75% UIHC. In the CN brains LB were absent (<1% UIHC). Increased [¹⁸F]FAZIN3 binding to MAO-A in AC was observed in all PD subjects. [¹⁸F]FAZIN3 ratio in PD was AC/CC = 3.57 while in CN subjects it was AC/CC = 2.24. Increases in UIHC μm² correlated with [¹⁸F]FAZIN3 binding to MAO-A in DLU/mm². Increased [¹⁸F]FAZIN3 binding to MAO-A in PD is a potential novel “hot spot” PET imaging approach. Full article

The protozoan parasite Cryptocaryon irritans causes marine white spot disease in a wide range of fish hosts, including gilthead seabream, a very sensitive species with great economic importance in the Mediterranean area. Thus, we aimed to evaluate the immunity of gilthead seabream after a severe natural outbreak of C. irritans. Morphological alterations and immune cell appearance in the gills were studied by light microscopy and immunohistochemical staining. The expression of several immune-related genes in the gills and head kidney were studied by qPCR, including inflammatory and immune cell markers, antimicrobial peptides (AMP), and cell-mediated cytotoxicity (CMC) molecules. Serum humoral innate immune activities were also assayed. Fish mortality reached 100% 8 days after the appearance of the C. irritans episode. Gill filaments were engrossed and packed without any space between filaments and included parasites and large numbers of undifferentiated and immune cells, namely acidophilic granulocytes. Our data suggest leukocyte mobilization from the head kidney, while the gills show the up-regulated transcription of inflammatory, AMPs, and CMC-related molecules. Meanwhile, only serum bactericidal activity was increased upon infection. A potent local innate immune response in the gills, probably orchestrated by AMPs and CMC, is triggered by a severe natural outbreak of C. irritans. Full article

Green nephrites are widely pursued for their mild texture and vivid color. In recent years, many Russian green nephrites appeared in China (the world’s largest nephrite market) and competed with the Chinese Manas green nephrites, which are traditionally highly valued. In this study, we compared the appearance, mineralogy and geochemical features (with EPMA and LA-ICP-MS) of Chinese (Manas) and Russian (East Sayan) green nephrites to objectively characterize and distinguish between these two nephrites. Chinese (Manas) and Russian (East Sayan) green nephrites are mined from serpentinized ultramafic units in the northern Tian Shan and East Sayan orogen, respectively. In terms of appearance, the Manas green nephrites are slightly bluish or grayish, whilst their East Sayan counterparts are brighter (duck-egg cyan). The Manas nephrites commonly have a caramel color, crumple structure, characteristic white globules and sinuous veins, green stains and yellow–green veins, together with a local fibrous structure. The East Sayan green nephrites are more transparent, with a gentler fine texture, uniform color, many black spots and a few green spots. Some green nephrites from the Arahushun mine of East Sayan have an ice-like appearance. Microscopic petrography and EPMA analysis indicate that both the Manas and East Sayan green nephrites comprise mainly tremolite with minor actinolite. Minor minerals in the Manas samples include chromite, chlorite-group minerals, and uvarovite; whilst those in the East Sayan samples include actinolite, chromite, chlorite-group minerals, and bornite. Bornite is not found in any other sources of green nephrite, and thus is characteristic of Russian (East Sayan) green nephrites. LA-ICP-MS trace element data in their amphiboles and Single-Factor Analysis of Variance (ANOVA) results suggest that the differences in Cr, Zn, Y, Ba, and Sr contents and values of δEu, Eu/Sm, (La/Yb)_N, (La/Sm)_N, (Gd/Yb)_N, ∑HREE, ∑LREE/∑HREE between the two nephrites are present, and can be used as their origin trace. Full article

The aim of the present study was to explore the feasibility of real-time monitoring and quantitative guiding the repair of enamel white spot lesions (WSLs) with resin infiltration by optical coherence tomography (OCT). Seven New Zealand rabbits were treated with 37% phosphoric acid etchant for 15 min to establish the model of enamel demineralization chalk spots of upper incisors, which were repaired by Icon resin infiltrant. OCT, stereo microscope (SM) imaging, scanning electron microscope (SEM) imaging and hematoxylin eosin (HE) staining were used to image each operation step. The changes of WSLs of enamel before and in the process of restoration with resin infiltrant showed specific performance in OCT images, which were consistent with the corresponding results of stereomicroscope and SEM. OCT can non-invasively and accurately image the whole process of repairing enamel demineralization layer with resin infiltration real-time, which can effectively guide the clinical use of resin infiltrant to repair enamel WSLs and be used as an imaging tool to evaluate the process and effect of restoration with resin infiltrant at the same time. Full article