Search Results (2)

Carvacrol, a phenolic monoterpene predominantly found in Origanum species, has been reported to exhibit antimicrobial, anti-inflammatory, antioxidant, and cytotoxic effects. Formulations such as Vacrol and S-Mix, enriched with carvacrol and complementary essential oil compounds, may enhance therapeutic efficacy while reducing toxicity. Essential oil components were analyzed via GC-MS. Cell viability was assessed using the sulforhodamine B (SRB) assay at different concentrations and incubation periods. An in ovo chorioallantoic membrane (CAM) assay was performed to investigate tumor volume changes and histopathological alterations. Vacrol and S-Mix demonstrated concentration- and time-dependent cell viability-attenuating effects in MDA-MB-231 cells, with significant reductions in viability at higher concentrations (1–10 mM). In ovo, S-Mix induced ~40% reduction in tumor volume and promoted apoptotic morphology compared to controls. Combined effects of carvacrol with α-pinene, eugenol, and β-terpineol likely contributed to enhanced bioactivity. These findings support further preclinical and mechanistic investigations to validate their therapeutic potential. Full article

Wound healing is a complex biological process involving overlapping phases of inflammation, proliferation, and remodeling. Plant-derived agents have gained attention as alternatives or adjuncts to synthetic drugs owing to their accessibility and favorable safety profile. This study evaluated the wound-healing activity of Vacrol Oil Combination (VOC), a phytotherapeutic preparation, through in vivo wound models and in vitro enzyme inhibition assays. Linear incision wounds in rats and circular excision wounds in mice were treated with VOC, administered orally, topically, or in combination for 10 days. Experimental groups included a negative control (no treatment), a vehicle control (olive oil), VOC-treated groups, and a reference group treated with 0.2% nitrofurazone. Wound contraction, tensile strength, histopathology, and hydroxyproline levels were assessed. In vitro assays were conducted to evaluate the inhibitory effects of VOC on hyaluronidase, collagenase, and elastase. VEGF and TGF-β1 levels were measured to assess the involvement of growth factors in the healing process. The chemical composition of VOC was characterized by gas chromatography–mass spectrometry (GC–MS), which identified carvacrol as the major compound, together with 1,8-cineole, linalool, eugenol, and cinnamaldehyde as prominent constituents known for their anti-inflammatory and antioxidant activities. VOC treatment significantly enhanced wound contraction and tensile strength compared to controls, with the oral + topical group showing the highest efficacy. Hydroxyproline levels and histological findings confirmed improved collagen synthesis and tissue regeneration. GC–MS analysis identified carvacrol as the major constituent of VOC, along with eugenol and linalool, which are known for their anti-inflammatory and antioxidant effects. Furthermore, VOC increased tissue levels of VEGF and TGF-β1, suggesting a role in stimulating angiogenesis and extracellular matrix remodeling. These findings indicate that the phytoconstituents of VOC, particularly carvacrol and oxygenated terpenes, act synergistically to promote wound repair. VOC demonstrates strong potential as a complementary phytotherapeutic agent for wound management, warranting further clinical investigation. Full article