Search Results (12)

Agricultural employment in advanced economies has been suggestively described as a “short and step” pyramid with only limited opportunities for workers to climb to higher positions, especially when they belong to the most vulnerable categories, such as migrants. The presence of poor jobs and living conditions for temporary agricultural workers reported by mass media, trade unions, NGOs, and international observers contrasts dramatically with the idea of a modern and thriving sector, as the Italian agricultural sector should be, and it represents a challenge for the social concerns that have been alongside environmental ones in the definition of sustainability since the Brundtland Report released in 1987 by the World Commission on Environment and Development (WCED). Even mechanisation does not necessarily facilitate better working conditions. In fact, where the workforce is largely replaced by machineries, the remaining opportunities for paid workers are mainly for unskilled, physically demanding, and seasonal jobs. This has brought about the so-called “paradox of prosperity”, where the gap between farm and nonfarm workers in economically advanced countries has widened in terms of wages, benefits, and prospects for upward mobility. This in turn triggers a vicious circle with a structural lack of available workforce for the sector, which has been increasingly provided by migrants with very little bargaining power. On the other hand, the adoption of new technologies and digitalisation in agriculture is leading to an increasing demand for skilled workers, which often remains uncovered because of the low conditions offered. Against this background, the aim of our work is twofold. First, we characterise the role of the foreign workforce in relation to the structural changes in Italian agriculture and considering territorial differences. Second, we examine the main policy instruments to facilitate recruitment and tackle undeclared work and more specifically the Quality Agricultural Work Network (Rete del lavoro agricolo di qualità) launched in Italy in 2016 to tackle undeclared work and exploitative labour. We do so by conducting a literature review and semi-structured qualitative interviews with 16 farmers in Italy carried out in 2022 within the project Rural Social ACT funded by the Asylum, Migration, and Integration Fund (2021–2027). The results show that even though foreign workers are a key resource for agriculture in Italy, there remain severe recruitment issues and segregation in low-skilled and precarious jobs. Overall, it is necessary to improve the awareness of the key role of work in agriculture and to strengthen the effectiveness of tools to enhance the visibility of compliant farms. So far (January 2024), only 6600 farms have joined the Quality Agricultural Work Network, with an overall modest enthusiasm from the farmers interviewed about its current effectiveness. Other instruments are explored such as employee sharing contracts, and there clearly emerges a need for public support of professional training through the Common Agricultural Policy to improve both the bargaining power of foreign workers and the productivity of the sector. Full article

Hen’s egg allergy is the second most common food allergy among infants and young children. The possible presence of undeclared eggs in foods poses a significant risk to sensitized individuals. Therefore, reliable egg allergen detection methods are needed to ensure compliance with food labeling and improve consumer protection. This work describes for the first time the application of phage display technology for the generation of a recombinant antibody aimed at the specific detection of hen’s ovomucoid. First, a single-chain variable fragment (scFv) library was constructed from mRNA isolated from the spleen of a rabbit immunized with ovomucoid. After rounds of biopanning, four binding clones were isolated and characterized. Based on the best ovomucoid-binding candidate SR-G1, an indirect phage enzyme-linked immunosorbent assay (phage-ELISA) was developed, reaching limits of detection and quantitation of 43 and 79 ng/mL of ovomucoid, respectively. The developed ELISA was applied to the analysis of a wide variety of food products, obtaining a good correlation with a commercial egg detection assay used as a reference. Finally, in silico modeling of the antigen-antibody complex revealed that the main interactions most likely occur between the scFv heavy chain and the ovomucoid domain-III, the most immunogenic region of this allergen. Full article

Undeclared work is a composite socioeconomic matter severely affecting the welfare of workers, legitimate companies, and the state by issuing unfair competition in the labour market and causing considerable state revenue losses by tax evasion. Labour inspectorates are tasked to deal effectively with this issue but usually lack adequate resources and proper tools, yet they own large volumes of past inspection data that, if aptly processed through innovative machine learning techniques, may produce understandable insights into the extent and prevailing patterns of undeclared work and efficient tools to address it. Such datasets are typically imbalanced regarding undeclared work, and contain overlapping inspection discoveries, two issues that impede the learning process. This research points to the problems of class imbalance and class overlap in this domain and applies combinations of data engineering techniques to address them using a dataset of 16.7 K actual labour inspections. Three associative classification algorithms are employed, and multiple classifiers are built and assessed for their predictability and interpretability. The study indicates the overall benefits for the inspection authorities when integrating machine learning methods in targeting undeclared work and proves considerable prediction performance improvement when following data engineering approaches to address the class imbalance and class overlap issues. Full article

Phthalates are chemicals that are extensively used in the manufacturing of cosmetic products. The occurrence of phthalate esters in personal care products may pose adverse effects on consumers’ health. In this work, a simple, fast and reliable GC-MS method was developed and validated for concurrent determination of phthalate esters in fragrances. Simple procedures were employed for sample preparation and clean up. The recoveries achieved were in the range of 94.9% to 105.6% with RSD ≤ 4.06. The detection limits were in the range of 0.0010 to 0.0021 µg/mL. The GC-MS method was utilized to investigate the occurrence of phthalate esters in different brands of perfumes sold in the Saudi Arabian market. Diethyl phthalate was detected in all analyzed samples, with a maximum concentration of 5766 µg/mL, and di (2-ethylhexyl) phthalate was detected in the majority of the analyzed samples (95%), with a mean concentration of 55.9 µg/mL and a highest concentration of 377.7 µg/mL. Additionally, the exposure to phthalate esters due to the consumption of perfumes was investigated among the adult Saudi population for the first time. It was found that the systemic exposure dose, measured at mean concentrations, ranged from 4.59 × 10⁻⁴ to 4.29 × 10⁻² (mg/kg/day) and from 5.00 × 10⁻⁴ to 4.68 × 10⁻² (mg/kg/day) for male and female users, respectively. Moreover, the non-carcinogenic risk of the investigated phthalate esters and the carcinogenic risk of DEHP were also evaluated. The non-carcinogenic risk values of the detected phthalate esters were greater than 100, which indicates that exposure to these phthalate esters is unlikely to produce non-carcinogenic health effects to consumers. However, at maximum DEHP concentrations, the carcinogenic risk values were 5.49 × 10⁻⁵ for male users and 5.98 × 10⁻⁵ for female users, which indicates the possibility of DEHP to pose a carcinogenic health effect if present at high levels. Regular monitoring of undeclared chemicals such as phthalate esters in personal care products marketed in Saudi Arabia is extremely important to ensure consumers’ safety. To the best of the authors’ knowledge, this is the first study to assess the health risk associated with consumption of perfumes in Saudi Arabia. Full article

Microbial bioformulations, due to their positive impact on the growth and development of plants, as well as the absence of harmful effects on the environment and humans, have a vast potential for mass introduction into agriculture. Assessing the quality of bioformulations, especially complex ones, is a difficult task. In this study, we show that high-throughput sequencing can be an effective tool for the quality control and safety of microbial bioformulations. By the method of high-throughput sequencing on the MiSeq platform, we studied 20 samples of commercially available microbial bioformulations. In parallel with this, bioformulations were studied by classical microbiological methods. The analysis showed the presence of extraneous undeclared bacterial genera by the manufacturer. Only 10% of the bioformulations fully corresponded to the commercial composition, and another 10% of the bioformulations did not contain the bacteria declared by the manufacturer in their composition at all. The bacterial composition of 80% of the bioformulations partially corresponded to the composition indicated on the package. The most frequent microbial bioformulations contaminants were Enterococcus, Lactobacillaceae, Klebsiella, Escherichia-Shigella and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium. Universal methods for the quality control of bioformulations are needed. The advantages of high-throughput sequencing for the evaluation of bioformulations are considered in this work. Full article

We consider the peculiarity of unique events, such as those of a natural, evolutionary, and social nature. In particular, we consider unique social events that have had either the claim or the vocation of being salvific for humanity, such as the introduction over time of the Torah, Hinduism, Buddhism, Christianity, and Islam. We question how the claimed, general salvific vocation contrasts, or is inconsistent with, the non-retroactive temporality and locality of such events, which could not have happened otherwise. This undeclared and philosophically unsolved inconsistency then reappears in subsequent cultural contradictions and inadequacies, political and social allowances such as, for instance, homo-centrism and a pathological relation with Nature. In the case of Christianity, this inconsistency is represented by the painting reproduced in the article, a work in which the excluded humans and other living beings are represented as astonished by the occurrence in this moment, and in such an unnatural context. Furthermore, we consider the original understanding as related to concepts of classical physics, or of such concepts naively adopted within the texts considered sacred. However, in some religions, such as Christianity, the inconsistency is theologically solved. We stress the need to update the ancient original elementary, naïve, pre-classic philosophical and conceptual frameworks used so that these alleged inconsistencies and contradictions may be not only theologically solved, but also conceptually solved in more complex understandings of the world, for example, considering relativistic time, long-range interdependence, quantum entanglement, and theories of the universe. Without this update, the unique saving events can affect only religiously, that is, optionally, on the scientific and philosophical conceptions used. Without this adjustment, homo-centrist illusion and egoism prevail as the natural, linear consequential attitude without raising these questions. It rather assumes that the intervention is for involved human beings, and moreover for those who have had and are lucky enough to receive and practice it, ignoring the enormous inconsistency within the message itself, and its presumed general and available salvific nature. This requires theological, philosophical, and scientific interdisciplinarity. The theme concerns inconsistencies within and superficiality of the narratives and their treatment of the unique, salvific events, without any reference to possible general and retroactive effects of how these events are represented in the painting. We conclude that the subject should be debated by taking into account contemporary understandings, such as relativistic space and time, quantum physics, and of the universe, with new philosophical and anthropological approaches. This should be a matter of responsible philosophical and theological interdisciplinary debate involving science, suitable to establish new understandings. Full article

The antifungal drug natamycin (NAT) is widely used in medicine and in the food industry as preservative E235 for a wide variety of foods. The risk of the development of resistance to NAT and its spread in relation to other polyene antibiotics is fraught with the emergence of incurable infections. This work is devoted to the development of an immunoassay to investigate the prevalence of NAT use for food preservation. Two immunogen designs based on tetanus toxoid, conjugated to NAT through different sites of hapten molecules, were compared in antibody generation. Assay formats using heterologous coating antigens were superior for both antibodies. The ELISA variant demonstrated the highest sensitivity (IC₅₀ = 0.12 ng/mL), and a limit of detection of 0.02 ng/mL was selected for NAT determination. The optimized extraction procedure provided a recovery rate of 72–106% for various food matrixes with variations below 12%. Cyclodextrins, as well as NAT–cyclodextrin complex formulations, showed no interference with the quantification of NAT. One hundred and six food product brands, including baked goods, wines, beers, drinks, sauces, and yogurts, were tested to assess the prevalence of the undeclared use of NAT as a preservative. The screening examination revealed three positive yogurts with an undeclared NAT incorporation of 1.1–9.3 mg/kg. Full article

Glyphosate (G)-based herbicidal formulations, such as the most commonly used one, Roundup (R), are major pesticides used worldwide on food and feed. Pregnant women may be frequently exposed to R compounds. These are composed of G, which is declared as the active principle, and other products contained in formulations, named formulants, which have been declared as inerts and diluents by the manufacturers. These formulants have, in fact, been demonstrated to be much more toxic than G, in particular to placental and embryonic human cells. In this work, we thus compared the effect of G and a GT+ formulation named R, using placental perfusion ex vivo. R, but not G alone, was demonstrated to alter the placental permeability of a known small model molecule, antipyrine. Similar results were observed for the fetal venous flow rate. The transfer of G alone increases with time, but is significantly decreased in presence of its formulants. The perfusion of R provokes a destruction of fetal vessels, as demonstrated by immunohistochemistry. Formulants obviously alter the fetal-placental circulation and placental integrity according to time of exposure. Therefore, G does not appear to be the main toxic agent of R. Formulants, although undeclared, include polyoxyethanolamines, PAHs, or heavy metals, and may be responsible for this toxicity. These compounds are also present in other pesticides. The progressive blood flow reduction due to the toxic compounds of formulations may diminish the nutrient supply to the fetus, alter the development, and may enhance the poisoning effects. Although these are preliminary results, they could at least partially explain some adverse pregnancy outcomes in mothers exposed to pesticides or other environmental pollutants. The debate on glyphosate alone is proven insufficient for the understanding of the toxicity. Full article

Those engaging in tax non-compliance have been conventionally explained as rational economic actors partaking when the benefits outweigh the costs, and thus public administrations have sought to enforce compliance using a deterrence approach which increases the risk of detection and penalties. However, many have been found to not engage in tax non-compliance when the benefits exceed the costs. The result has been the emergence of a voluntary compliance approach viewing taxpayers as social actors who engage in tax non-compliance when there is a lack of vertical trust (in governments) and horizontal trust (in others). Using a probit regression analysis of data from special Eurobarometer surveys conducted in 2007, 2013 and 2019, the finding is that although the likelihood of participating in tax non-compliance is largely not associated with the level of penalties and risk of detection, it is significantly associated with the level of vertical and horizontal trust, with participation in tax non-compliance increasing with lower vertical and horizontal trust. The implications for theory and for how public administrations tackle tax non-compliance are then discussed. Full article

Most empirical work in the social sciences is based on observational data that are often both incomplete, and therefore unrepresentative of the population of interest, and affected by measurement errors. These problems are very well known in the literature and ad hoc procedures for parametric modeling have been proposed and developed for some time, in order to correct estimate’s bias and obtain consistent estimators. However, to our best knowledge, the aforementioned problems have not yet been jointly considered. We try to overcome this by proposing a parametric approach for the estimation of the probabilities of misclassification of a binary response variable by incorporating them in the likelihood of a binary choice model with sample selection. Full article

The Sustainable Development Goals (SDGs) that were established by the United Nations for the year 2030 are considered the blueprint for reaching a sustainable future through addressing poverty eradication, education, social protection, environmental protection, climate change, health, job opportunities, and strong institutions. Until now, sustainable development has focused mainly on the formal sector, somehow neglecting the potential contributions of the informal sector to sustainable futures. In this context, the paper aims to analyze the main causes of the Romanian shadow economy (SE) both at the micro and macro levels, and how they can make a considerable impact on achieving a more sustainable future. The main purpose of the paper is to highlight the relevance of knowing the main determinants of this phenomenon, emphasizing that keeping the incidence of the shadow economy and its causes under control could offer an alternative in the process of achieving sustainable development. In order to do that, we use an improved MIMIC (Multiple Causes Multiple Indicators) specification of the causal variables that is grounded in an evidence-based approach through using the points of view of the Romanian entrepreneurs. Their opinions are based on a national representative survey that is based on quarterly data covering the period 2000–2017. The empirical analysis pointed out the roles of tax avoidance, bribery, a lack of trust in public officials, the poor quality of business legislation, the poor government support for entrepreneurs, high social contributions, a relatively high of corruption, high tax rates, and uncertainty about regulation policies in increasing incidences of shadow economic activity. The macroeconomic results that were revealed to be the main causes for the Romanian SE were self-employment, unemployment, part-time employment, and the lack of trust in public institutions. Therefore, policymakers need to focus on building reliable and transparent institutions with a lower level of corruption, regulations, and bureaucracy, regaining people’ confidence in public institutions, and elaborating effective strategies for tackling the undeclared activities that will contribute to the achievement of sustainable development desideratum by the fulfillment of two main SDGs. These are Goal 8: Decent work and economic growth, and Goal 16: Peace, justice, and strong institutions. Full article

Frankfurters are widely consumed all over the world, and the production requires a wide range of meat and non-meat ingredients. Due to these characteristics, frankfurters are products that can be easily adulterated with lower value meats, and the presence of undeclared species. Adulterations are often still difficult to detect, due the fact that the adulterant components are usually very similar to the authentic product. In this work, FT-Raman spectroscopy was employed as a rapid technique for assessing the quality of frankfurters. Based on information provided by the Raman spectra, a multivariate classification model was developed to identify the frankfurter type. The aim was to study three types of frankfurters (chicken, turkey and mixed meat) according to their Raman spectra, based on the fatty vibrational bands. Classification model was built using partial least square discriminant analysis (PLS-DA) and the performance model was evaluated in terms of sensitivity, specificity, accuracy, efficiency and Matthews’s correlation coefficient. The PLS-DA models give sensitivity and specificity values on the test set in the ranges of 88%–100%, showing good performance of the classification models. The work shows the Raman spectroscopy with chemometric tools can be used as an analytical tool in quality control of frankfurters. Full article