A Glyphosate-Based Formulation but Not Glyphosate Alone Alters Human Placental Integrity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Chemical Analyses
2.2. Human Tissues and Ethics
2.3. Placental Perfusion
2.4. Calculation of Placental Parameters
2.5. Immunohistochemistry
2.6. Statistics
3. Results
3.1. Transplacental Transfer of A and Fetal Venous Flow in Presence of G Alone or with Its Formulants (R)
3.2. G Transplacental Transfer Rate Alone or in Its Formulants (R) in Comparison to A
3.3. Placental Histology after 240 min Perfusion of G or R
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
Abbreviations
References
- Benbrook, C.M. Trends in glyphosate herbicide use in the United States and globally. Environ. Sci. Eur. 2016, 28, 3. [Google Scholar] [CrossRef] [Green Version]
- Seralini, G.-E. Update on long-term toxicity of agricultural GMOs tolerant to roundup. Environ. Sci. Eur. 2020, 32, 18. [Google Scholar] [CrossRef] [Green Version]
- Malalgoda, M.; Ohm, J.-B.; Ransom, J.K.; Howatt, K.; Simsek, S. Effects of pre-harvest glyphosate application on spring wheat quality characteristics. Agriculture 2020, 10, 111. [Google Scholar] [CrossRef] [Green Version]
- Carles, L.; Gardon, H.; Joseph, L.; Sanchís, J.; Farre, M.; Artigas, J. Meta-analysis of glyphosate contamination in surface waters and dissipation by biofilms. Environ. Int. 2019, 124, 284–293. [Google Scholar] [CrossRef] [PubMed]
- Connolly, S.J.; Crowther, M.; Eikelboom, J.W.; Gibson, C.M.; Curnutte, J.T.; Lawrence, J.H.; Yue, P.; Bronson, M.D.; Lu, G.; Conley, P.B.; et al. Full study report of andexanet alfa for bleeding associated with factor Xa inhibitors. N. Engl. J. Med. 2019, 380, 1326–1335. [Google Scholar] [CrossRef] [PubMed]
- Fagan, J.; Bohlen, L.; Patton, S.; Klein, K. Organic diet intervention significantly reduces urinary glyphosate levels in US children and adults. Environ. Res. 2020, 189, 109898. [Google Scholar] [CrossRef]
- Mesnage, R.; Mazzacuva, F.; Caldwell, A.; Halket, J.; Antoniou, M.N. Urinary excretion of herbicide co-formulants after oral exposure to roundup MON 52276 in rats. Environ. Res. 2021, 197, 111103. [Google Scholar] [CrossRef]
- Richard, S.; Moslemi, S.; Sipahutar, H.; Benachour, N.; Seralini, G.-E. Differential effects of glyphosate and roundup on human placental cells and aromatase. Environ. Health Perspect. 2005, 113, 716–720. [Google Scholar] [CrossRef] [Green Version]
- Séralini, G.-E. Génétiquement Incorrect; Flammarion: Paris, France, 2015. [Google Scholar]
- Benachour, N.; Séralini, G.-E. Glyphosate formulations induce apoptosis and necrosis in human umbilical, embryonic, and placental cells. Chem. Res. Toxicol. 2009, 22, 97–105. [Google Scholar] [CrossRef]
- Defarge, N.; Takács, E.; Lozano, V.L.; Mesnage, R.; De Vendômois, J.S.; Séralini, G.-E.; Székács, A. Co-formulants in glyphosate-based herbicides disrupt aromatase activity in human cells below toxic levels. Int. J. Environ. Res. Public Health 2016, 13, 264. [Google Scholar] [CrossRef] [Green Version]
- Defarge, N.; de Vendômois, J.S.; Séralini, G. Toxicity of formulants and heavy metals in glyphosate-based herbicides and other pesticides. Toxicol. Rep. 2018, 5, 156–163. [Google Scholar] [CrossRef]
- Seralini, G.-E.; Jungers, G. Toxic compounds in herbicides without glyphosate. Food Chem. Toxicol. 2020, 146, 111770. [Google Scholar] [CrossRef]
- Séralini, G.-E.; Clair, E.; Mesnage, R.; Gress, S.; Defarge, N.; Malatesta, M.; Hennequin, D.; De Vendômois, J.S. Republished study: Long-term toxicity of a Roundup herbicide and a Roundup-tolerantgenetically modified maize. Environ. Sci. Eur. 2014, 26, 14. [Google Scholar] [CrossRef] [Green Version]
- Larsen, E.A.; Gaines, S.D.; Deschênes, O. Agricultural pesticide use and adverse birth outcomes in the San Joaquin Valley of California. Nat. Commun. 2017, 8, 302. [Google Scholar] [CrossRef] [Green Version]
- Mai, C.T.; Isenburg, J.L.; Canfield, M.A.; Meyer, R.E.; Correa, A.; Alverson, C.J.; Lupo, P.J.; Riehle-Colarusso, T.; Cho, S.J. National population-based estimates for major birth defects, 2010–2014. Birth Defects Res. 2019, 111, 1420–1435. [Google Scholar] [CrossRef]
- Ventura, S.J.; Curtin, S.C.; Abma, J.C.; Henshaw, S.K. Estimated pregnancy rates and rates of pregnancy outcomes for the United States, 1990–2008. National vital statistics reports: From the centers for disease control and prevention. Natl. Vital Stat. Syst. 2012, 60, 1–21. [Google Scholar]
- Parvez, S.; Parvez, S.; Gerona, R.R.; Proctor, C.; Friesen, M.; Ashby, J.L.; Reiter, J.L.; Lui, Z.; Winchester, P.D. Glyphosate exposure in pregnancy and shortened gestational length: A prospective Indiana birth cohort study. Environ. Health 2018, 17, 23. [Google Scholar] [CrossRef] [PubMed]
- Shelton, J.F.; Geraghty, E.M.; Tancredi, D.J.; Delwiche, L.D.; Schmidt, R.; Ritz, B.; Hansen, R.L.; Hertz-Picciotto, I. Neurodevelopmental disorders and prenatal residential proximity to agricultural pesticides: The CHARGE study. Environ. Health Perspect. 2014, 122, 1103–1109. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Patel, D.M.; Jones, R.R.; Booth, B.J.; Olsson, A.C.; Kromhout, H.; Straif, K.; Vermeulen, R.; Tikellis, G.; Paltiel, O.; Golding, J.; et al. Parental occupational exposure to pesticides, animals and organic dust and risk of childhood leukemia and central nervous system tumors: Findings from the International Childhood Cancer Cohort Consortium (I4C). Int. J. Cancer 2020, 146, 943–952. [Google Scholar] [CrossRef]
- Séralini, G.; Douzelet, J. The Taste of Pesticides in Wines. Food Nutr. J. 2017, 2, 161. [Google Scholar] [CrossRef] [Green Version]
- Mesnage, R.; Bernay, B.; Séralini, G.-E. Ethoxylated adjuvants of glyphosate-based herbicides are active principles of human cell toxicity. Toxicology 2013, 313, 122–128. [Google Scholar] [CrossRef]
- Ceccaldi, P.-F.; Gavard, L.; Mandelbrot, L.; Rey, E.; Farinotti, R.; Treluyer, J.-M.; Gil, S. Functional role of p-glycoprotein and binding protein effect on the placental transfer of lopinavir/ritonavir in the ex vivo human perfusion model. Obstet. Gynecol. Int. 2009, 2009, e0220323. [Google Scholar] [CrossRef] [Green Version]
- Kar, A. Medicinal Chemistry; New Age International: Hyderabad, India, 2005. [Google Scholar]
- Challier, J.-C. Criteria for evaluating perfusion experiments and presentation of results. In vitro perfusion of human placental tissue. Contrib. Gynecol. Obstet. 1985, 13, 32–39. [Google Scholar] [PubMed]
- Mose, T.; Kjaerstad, M.B.; Mathiesen, L.; Nielsen, J.B.; Edelfors, S.; Knudsen, L.E. Placental passage of benzoic acid, caffeine, and glyphosate in an ex vivo human perfusion system. J. Toxicol. Environ. Health Part A 2008, 71, 984–991. [Google Scholar] [CrossRef] [PubMed]
- Sahni, M.; Vibert, Y.; Bhandari, V.; Menkiti, O. Newborn infant with mothball toxicity due to maternal ingestion. Pediatrics 2019, 143, e20183619. [Google Scholar] [CrossRef] [PubMed]
- Dallegrave, E.; Mantese, F.D.; Oliveira, R.T.; Martino-Andrade, A.; Dalsenter, P.; Langeloh, A. Pre-and postnatal toxicity of the commercial glyphosate formulation in Wistar rats. Arch. Toxicol. 2007, 81, 665–673. [Google Scholar] [CrossRef]
- Vanlaeys, A.; Dubuisson, F.; Seralini, G.-E.; Travert, C. Formulants of glyphosate-based herbicides have more deleterious impact than glyphosate on TM4 Sertoli cells. Toxicol. In Vitro 2018, 52, 14–22. [Google Scholar] [CrossRef] [PubMed]
- Benirschke, K.; Burton, G.J.; Baergen, R.N. Anatomy and pathology of the placental membranes. In Pathology of the Human Placenta; Springer: Berlin/Heidelberg, Germany, 2012; pp. 249–307. [Google Scholar]
- Berveiller, P.; Degrelle, S.; Segond, N.; Cohen, H.; Evain-Brion, D.; Gil, S. Drug transporter expression during in vitro differentiation of first-trimester and term human villous trophoblasts. Placenta 2015, 36, 93–96. [Google Scholar] [CrossRef]
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Simasotchi, C.; Chissey, A.; Jungers, G.; Fournier, T.; Seralini, G.-E.; Gil, S. A Glyphosate-Based Formulation but Not Glyphosate Alone Alters Human Placental Integrity. Toxics 2021, 9, 220. https://doi.org/10.3390/toxics9090220
Simasotchi C, Chissey A, Jungers G, Fournier T, Seralini G-E, Gil S. A Glyphosate-Based Formulation but Not Glyphosate Alone Alters Human Placental Integrity. Toxics. 2021; 9(9):220. https://doi.org/10.3390/toxics9090220
Chicago/Turabian StyleSimasotchi, Christelle, Audrey Chissey, Gérald Jungers, Thierry Fournier, Gilles-Eric Seralini, and Sophie Gil. 2021. "A Glyphosate-Based Formulation but Not Glyphosate Alone Alters Human Placental Integrity" Toxics 9, no. 9: 220. https://doi.org/10.3390/toxics9090220