Search Results (15)

Temporomandibular joint replacement is a critical intervention for severe temporomandibular joint disorders, enhancing pain levels, jaw function and overall quality of life. In this study, we compare two finite element method-based simulation workflows from both academic and industrial perspectives, focusing on a patient-specific case involving a custom-made temporomandibular joint prosthesis. Using computed tomography data and computer-aided design data, we generated different 3D models and performed mechanical testing, including wear and static compression tests. Our results indicate that the academic workflow, which is retrospective, purely image-based and applied post-operatively, produced peak stress values within 9–20% of those obtained from the industrial workflow. The industrial workflow is prospective, pre-operative, computer-aided design-based and guided by stringent regulatory standards and approval protocols. Observed differences between workflows were attributed primarily to distinct modelling assumptions, simplifications and constraints inherent in each method. To explicitly quantify these differences, multiple additional models were generated within the academic workflow using partial data from the industrial process, revealing specific sources of variation in stress distribution and implant performance. The findings underscore the potential of patient-specific simulations not only to refine temporomandibular joint prosthesis design and enhance patient outcomes, but also to highlight the interplay between academic research methodologies and industrial standards in the development of medical devices. Full article

To address roof overhang hazards (e.g., rock bursts and gas accumulation) in high-gas coal mines, this study proposes a static stress intervention method for controlled directional roof collapse. Using the 150110 fully mechanized face at Yiyuan Coal Mine as a case study, we investigate the mechanical mechanism of static stress intervention-induced roof collapse through theoretical modeling and FLAC3D simulations in the absence of pre-cracks. The study reveals that advanced boreholes filled with static expansion agents generate stress concentration zones along the drilling array. When superimposed with mining-induced stresses, this configuration induces tensile failure preferentially at borehole locations, thereby achieving controlled directional roof collapse. Theoretical calculations indicate that roof fracturing occurs at predetermined locations when expansion pressure reaches ≥9.11 MPa. FLAC3D simulations analyzed stress redistribution and plastic zone evolution under combined static and mining-induced stresses, demonstrating the method’s efficacy in optimizing roadway stability. Field trials implement spaced boreholes (65 mm diameter, 16 m depth, 1 m spacing) with alternating expansion agent charging, achieving a 6 m reduction in roof collapse intervals, effectively mitigating overhang hazards. Results confirm that static stress intervention reshapes the roof stress field, inducing tensile failure along predetermined paths without relying on pre-cracks. The findings provide theoretical and technical insights for roof stability control in high-gas coal mines. Full article

This study investigates structural integrity and proposes retrofitting solutions for the historical two-storey school building in Thrapsano, Crete, severely impacted by the September 2021 earthquake. An extensive methodology was adopted, incorporating field surveys, material characterization, finite element modeling, and experimental analysis. The assessment is focused on identifying structural damage, such as cracking and delamination in masonry walls, and evaluating the dynamic and static performance of the load-bearing system under seismic loads. Key interventions include grouting for masonry reinforcement, replacement of mortar with compatible materials, stitching of cracks, and the addition of reinforced concrete and metallic tie elements to enhance diaphragm action. Advanced numerical simulations, validated through experimental data, were employed to model the pre- and post-retrofit behavior of the structure. The proposed retrofitting measures align with Eurocodes 6 and 8, and the Greek code for masonry structures (KADET), aiming to restore the structural stability and improve seismic resilience while respecting the building’s historical significance. The results from the finite element analysis confirm the effectiveness of the interventions in reducing tensile stresses and improving load redistribution, ensuring compliance with modern safety standards. This case study offers a framework for the seismic retrofitting of heritage structures in a similar context. Full article

The phosphorus-31 (³¹P) spectral modulus (PSM) is a measure of the metabolic status of cells, tissues, and organs. The PSM can be calculated from ³¹P nuclear magnetic resonance (³¹P NMR) spectra obtained from cell, tissue, or organ preparations. These ³¹P NMR spectra can be a measure of intact living cells, tissues, or organs, or appropriate biochemical extracts of such preparations. The ³¹P NMR spectrum is comprised of signals derived from organophosphate metabolites that resonate from 10 δ to −25 δ on the phosphorus chemical shift δ scale. The PSM is the ratio of the high-energy phosphate to that of the low-energy phosphate spectral integrals. These integrals may be conveniently grouped into high-energy and low-energy spectral regions, respectively, into ³¹P chemical shifts located between −0.13 δ to −25 δ and between 10 δ to −0.13 δ. High-energy phosphates are typically described as providing the energy necessary for the activity of cellular metabolism; chemically, they contain one or more phosphate anhydride bonds. This study demonstrates that, (1) in general, the higher the metabolic activity, the higher the PSM, and (2) the modulus calculation does not require a highly resolved ³¹P spectrum and can be calculated solely from the integral. The PSM was calculated among cells, tissues, and organs considered normal, diseased, and stressed. In diseased (mean 1.29 ± 0.73) and stressed (mean 1.23 ± 0.75) cells, tissues, and organs, PSM values are typically low or low relative to normal cells, tissues, or organs (mean 1.65 ± 0.90), following time-course measurements, in dynamic decline. The PSM is useful in determining the metabolic status of cells, tissues, or organs and can be employed as a calculable numeric assay for determining health status statically or over time. Calculation of the PSM can be carried out with spectra of low signal-to-noise; it relies on the minimal resolution required to detect an integral curve having a clear spectral integral inflection point at ca. −0.13 δ. Detection of an integral curve alone enables the calculation of a PSM even at levels of phosphorus concentration so low as to prevent detection of the individual or groups of metabolites, such as with in vivo or ex vivo cell, tissue, or organ determinations. This study (1) presents the foundations and fundamentals of the PSM, a living index of tissue metabolic health, and (2) demonstrates the use of spectral scan analysis in opening new vistas of biology and medicine for measuring the metabolic status of stressed and diseased tissues at a range of detectable levels for monitoring therapeutic interventions. Full article

The use of minimalist shoes can lead to enhanced foot somatosensory activation and postural stability but can also increase the incidence of overuse injuries during high-impact or prolonged activities. Therefore, it appears useful to explore new strategies that employ minimalist shoes to effectively facilitate the somatosensory activation of the foot while minimizing acute and cumulative joint stress and risk of injury. To this purpose, this study introduces a novel exercise paradigm: walking for three minutes in ultra-minimalist shoes on artificial flat surfaces designed to mimic highly rugged natural terrains. The activity of foot muscles and lumbar multifidus, pain perception level, and stabilometric parameters were recorded and analyzed to characterize the novel exercise, comparing it to walking barefoot or in conventional shoes on the same rugged surface. Compared to being barefoot, ultra-minimalist shoes effectively filter nociceptive stimuli from the rugged surface, while compared to conventional shoes, they enhance the somatosensory input supporting static stability. Walking with ultra-minimalist and conventional shoes yielded higher gastrocnemius activity and lower tibialis anterior and multifidus activity compared to barefoot walking. This study highlights a practical and safe framework for enhancing foot somatosensory activation and postural stability. The new intervention is suitable for people of all ages, requires minimal time commitment, and can be performed in controlled environments such as homes, gyms, and healthcare facilities. Full article

Oxidative stress (OS) affects men’s health and impairs spermatogenesis. Micronutrient antioxidants are available for male infertility as complemental support; however, their efficacy remains debatable. This study aimed to investigate whether antioxidants can help to reduce sperm OS and improve semen analysis and quality. We included 171 male partners of couples planning to undergo assisted reproductive technology (ART). Male partners, aged 29–41 years, of couples intending to conceive were self-selected to take daily antioxidants (n = 84) containing folic acid and zinc, or not to take antioxidants (n = 52) for 6 months. We analyzed the alterations in serum oxidant levels, sperm parameters, OS, and deoxyribonucleic acid fragmentation after 3 and 6 months. Additionally, implantation, clinical pregnancy, and miscarriage rates after vitrified–warmed embryo transfer were compared between those taking antioxidants and those not taking them after 6 months. In men with high static oxidation–reduction potential (sORP), we observed a significant improvement in sperm concentration and sORP. The high-quality blastocyst rate tended to increase, and implantation and clinical pregnancy rates also significantly increased after 6 months of intervention. The micronutrient antioxidants could improve sperm function by reducing OS and improving ART outcomes. Therefore, micronutrient antioxidants may be a viable treatment option for male infertility. Full article

This clinical trial aims to provide evidence about the effectiveness of the Pilates method on stress urinary incontinence (SUI), as well as to elucidate the effects of photobiomodulation therapy associated with static magnetic field (PBMT/sMF) alone or associated with the Pilates Method on Pelvic floor muscle (PFM) in women affected by SUI. For that, a three-arm, parallel randomized, double-blinded, placebo-controlled trial was conducted (NCT05096936). We recruited thirty-three women diagnosed with SUI, randomly allocated to three groups: placebo PBMT/sMF plus method Pilates, PBMT/sMF active plus method Pilates and only PBMT/sMF active. The evaluation consisted of anamnesis and physical examination, muscle strength, completion of the ICIQ-SF questionnaire, and urinary loss. The evaluation of muscle strength and filling the ICIQ-SF were performed on the first and last days, while the Pad test was applied in baseline, one month, two months, and three months of intervention. We observed an increase in strength (p < 0.01), tone (p < 0.01), and quality of life (p < 0.01), in addition to a decrease in urinary lost (p < 0.01) for all groups comparing the pre and post-intervention. The PBMT/sMF alone, the Pilates, and the combination of the two therapies proved to be effective in improving the signs and symptoms of women with SUI. Full article

Objective: Negative moods in college students have caused frequent extreme behaviors. This study analyzed the current status and correlation between physical activity and negative moods in college students. Methods: A cross-sectional study design was used in the present research. Data on college students’ physical activity and negative moods were collected using the Sports Questionnaire Star software. The questionnaires were administered to college students in five colleges and universities in Henan and Jiangsu Provinces, China, and a total of 3711 correctly completed questionnaires were returned. Data on sociological and demographic information, the International Physical Activity Questionnaire Short Form (IPAQ-SF), and the Depression Anxiety and Stress Scale (DASS) were collected. The research was conducted in December 2021. Results: The low-intensity physical activity rate among college students was 55.56%, while depression, anxiety, and stress symptoms were detected in 35.14%, 65.29%, and 10.99%of the college students, respectively. Depression (K = 35.58, p < 0.001) and anxiety (K = 15.56, p < 0.001) rates were significantly different among the different physical activity intensity groups. The proportion of students who perform high-intensity physical activities was lower than those who perform low- and moderate-intensity physical activities. Conclusion: Low physical activity and high anxiety are evident among college students, and prolonged moderate-to-low-intensity physical activity (including static behavior) induces depression and anxiety. In the future, further studies can be conducted on improving the physical activity intensity of college students, improving the mental health monitoring and intervention systems of college students, and exploring the dose–effect relationship between physical activity and negative moods. Full article

The braking mode of the battery electric urban bus (BEUB) is different from the friction braking of the traditional fuel bus due to the introduction of a regenerative braking system. The intervention of electromagnetic braking changes the working condition of the main reducer gears, thus affecting their service lives. Based on the Urban Dynamometer Driving Schedule (UDDS) driving cycle condition, the stress–time history of the main reducer gears is calculated. Combined with the static analysis results and the S-N curve of the material, the fatigue lives of the main reducer gears considering electromagnetic braking and traditional friction braking are analyzed. The reverse torque on the driving axle during electromagnetic braking is taken into account to be closer to the real situation. Results show that, under the electromagnetic braking mode, the bending fatigue lives of the tooth root on the convex and concave surfaces of the pinion are 78.5% and 78.9% of that under the traditional friction braking mode, respectively, while the contact fatigue life of the pinion working surface is 78.2% of that under the friction braking mode, indicating that the introduction of the regenerative braking system into the BEUB will cause a significant reduction in the service life of the main reducer gears. This study provides a high-precision fatigue life calculation method for the BEUB main reducer gears and the accurate prediction of their remaining life. Full article

The mindfulness-based stress reduction (MBSR) programme is gaining increasing attention in sport and physical activity domains. This programme comprises three meditation practices: mindful yoga, body scan, and sitting meditation. In this study, we aimed to examine the effects of a dynamic (mindful yoga) strategy and a static (a combination of body scan/sitting meditation) strategy on participants’ psychobiosocial states (PBS), perceived stress (PS) and mindfulness levels in athletes and recreationally active (RA) people. Thirty-four participants (athletes = 18; RA participants = 16) were assigned to a dynamic intervention strategy, and another 34 (athletes = 19; RA participants = 15) were assigned to the static intervention strategy. Before the intervention, after the intervention and three weeks later, the Italian versions of the PBS scale, the PS scale and the Mindful Attention Awareness scale were administered. RM-(M)ANOVAs revealed that intervention strategies improved functional PBS, reduced PS and enhanced mindfulness levels in both athletes and RA participants after the intervention (p < 0.001, η_p² = 0.605). However, improved functional PBS after the intervention (p < 0.001; d = 0.62) and stable PS levels at follow-up (p = 1) were observed mainly in athletes. The findings reinforce the view of the importance of the body as a means to improve emotional and health processes, and support the use of mindfulness strategies in sport to enhance individuals’ well-being. Full article

Oxidative stress is associated with a myriad of diseases including pregnancy pathologies with long-term cardiovascular repercussions for both the mother and baby. Aberrant redox signalling coupled with deficient antioxidant defence leads to chronic molecular impairment. Abnormal placentation has been considered the primary source for reactive species; however, placental dysfunction has been deemed secondary to maternal cardiovascular maladaptation in pregnancy. While various therapeutic interventions, aimed at combating deregulated oxidative stress during pregnancy have shown promise in experimental models, they often result as inconclusive or detrimental in clinical trials, warranting the need for further research to identify candidates. The strengths and limitations of current experimental methods in redox research are discussed. Assessment of redox status and oxidative stress in experimental models and in clinical practice remains challenging; the state-of-the-art of computational models in this field is presented in this review, comparing static and dynamic models which provide functional information such as protein-protein interactions, as well as the impact of changes in molecular species on the redox-status of the system, respectively. Enhanced knowledge of redox biology in during pregnancy through computational modelling such as generation of Systems Biology Markup Language model which integrates existing models to a larger network in the context of placenta physiology. Full article

Vascular interventions result in the disruption of the tunica intima and the exposure of sub-endothelial matrix proteins. Nanoparticles designed to bind to these exposed matrices could provide targeted drug delivery systems aimed at inhibiting dysfunctional vascular remodeling and improving intervention outcomes. Here, we present the progress in the development of targeted liposomal nanocarriers designed for preferential collagen IV binding under simulated static vascular flow conditions. PEGylated liposomes (PLPs), previously established as effective delivery systems in vascular cells types, served as non-targeting controls. Collagen-targeting liposomes (CT-PLPs) were formed by conjugating established collagen-binding peptides to modified lipid heads via click chemistry (CTL), and inserting them at varying mol% either at the time of PLP assembly or via micellar transfer. All groups included fluorescently labeled lipid species for imaging and quantification. Liposomes were exposed to collagen IV matrices statically or via hemodynamic flow, and binding was measured via fluorometric analyses. CT-PLPs formed with 5 mol% CTL at the time of assembly demonstrated the highest binding affinity to collagen IV under static conditions, while maintaining a nanoparticle characterization profile of ~50 nm size and a homogeneity polydispersity index (PDI) of ~0.2 favorable for clinical translation. When liposomes were exposed to collagen matrices within a pressurized flow system, empirically defined CT-PLPs demonstrated significant binding at shear stresses mimetic of physiological through pathological conditions in both the venous and arterial architectures. Furthermore, when human saphenous vein explants were perfused with liposomes within a closed bioreactor system, CT-PLPs demonstrated significant ex vivo binding to diseased vascular tissue. Ongoing studies aim to further develop CT-PLPs for controlled targeting in a rodent model of vascular injury. The CT-PLP nanocarriers established here show promise as the framework for a spatially controlled delivery platform for future application in targeted vascular therapeutics. Full article

This study represents a comparison of the functional interrelation of fatigue and cognitive, cardiovascular and autonomic nervous systems in a group of Chronic Fatigue Syndrome (CFS) patients compared with those in healthy individuals at different stages of analysis: at baseline and after changes induced by whole-body cryotherapy (WBC) combined with a static-stretching (SS) program. The study included 32 patients (Fukuda criteria) and 18 healthy controls. Fatigue, cognitive, cardiovascular and autonomic function and arterial stiffness were measured before and after 10 sessions of WBC with SS. In the patients, a disturbance in homeostasis was observed. The network relationship based on differences before and after intervention showed comparatively higher stress and eccentricity in the CFS group: 50.9 ± 56.1 vs. 6.35 ± 8.72, p = 0.002, r = 0.28; and 4.8 ± 0.7 vs. 2.4 ± 1, p < 0.001, r = 0.46, respectively. Before and after intervention, in the CFS group increased fatigue was related to baroreceptor function, and baroreceptor function was in turn related to aortic stiffness, but no such relationships were observed in the control group. Differences in the network structure underlying the interrelation among the four measured criteria were observed in both groups, before the intervention and after ten sessions of whole cryotherapy with a static stretching exercise. Full article

Background: The use of surface electromyography (sEMG) measurements to evaluate the bioelectrical activity of the pelvic floor muscle (PFM) during therapeutic intervention is now well established. This study investigates the diagnostic possibilities of sEMG in women with stress urinary incontinence (SUI). The aim of this study was to carry out objective assessments of the bioelectrical activity of the PFM in women after menopause and determine the prognostic value of sEMG for assessing the PFM in patients with SUI. Methods: This was a prospective, observational study that evaluated the bioelectrical activity of the PFM in postmenopausal women with or without SUI (SUI group, n = 89 vs. non-SUI group, n = 62). The study was carried out between January 2013 and December 2018 at the Clinic of Urology (Wroclaw, Poland). The protocol for all sEMG measurements of PFM activity consisted of following elements: “baseline”, “quick flicks”, “contractions”, “static hold”, and “rest tone”; we then compared these results between groups. To determine the optimal cutoff level for sEMG activation of the PFM to detect the occurrence of SUI, we performed receiver operating characteristic (ROC) curve analysis (with Youden’s index). Results: Significantly lower results were obtained for all PFM measurements in women with SUI. The optimum diagnostic cutoff for “baseline” was 3.7 μV (area under curve (AUC), 0.63), “quick flicks” was 9.15 μV (AUC, 0.84), “contractions” was 11.33 μV (AUC, 0.80), “static hold” was 9.94 μV (AUC, 0.84), and “rest” was 3.89 μV (AUC, 0.63). Conclusions: Measuring sEMG activity in the PFM may be a useful diagnostic tool to confirm the absence of SUI. We can expect that the sEMG activity of subjects with SUI will be lower than that of healthy people. In order to determine appropriate reference values for assessing sEMG activity data in the PFM, it is now necessary to conduct multicenter studies. Full article

Autophagy (self-eating) is a conserved cellular degradation process that plays important roles in maintaining homeostasis and preventing nutritional, metabolic, and infection-mediated stresses. Autophagy dysfunction can have various pathological consequences, including tumor progression, pathogen hyper-virulence, and neurodegeneration. This review describes the mechanisms of autophagy and its associations with other cell death mechanisms, including apoptosis, necrosis, necroptosis, and autosis. Autophagy has both positive and negative roles in infection, cancer, neural development, metabolism, cardiovascular health, immunity, and iron homeostasis. Genetic defects in autophagy can have pathological consequences, such as static childhood encephalopathy with neurodegeneration in adulthood, Crohn’s disease, hereditary spastic paraparesis, Danon disease, X-linked myopathy with excessive autophagy, and sporadic inclusion body myositis. Further studies on the process of autophagy in different microbial infections could help to design and develop novel therapeutic strategies against important pathogenic microbes. This review on the progress and prospects of autophagy research describes various activators and suppressors, which could be used to design novel intervention strategies against numerous diseases and develop therapeutic drugs to protect human and animal health. Full article