Search Results (49)

Endometrial cancer (EC) is the most common gynecological malignancy in developed countries. Risk factors for EC include metabolic alterations (obesity, metabolic syndrome, insulin resistance), hormonal imbalance, age at menopause, reproductive factors, and inherited conditions, such as Lynch syndrome. For the inherited forms, several genes had been implicated in EC occurrence and development, such as POLE, MLH1, TP53, PTEN, PIK3CA, PIK3R1, CTNNB1, ARID1A, PPP2R1A, and FBXW7, all mutated at high frequency in EC patients. However, gene function impairment is not necessarily caused by mutations in the coding sequence of these and other genes. Gene function alteration may also occur through post-transcriptional control of messenger RNA translation, frequently caused by microRNA action, but transcriptional impairment also has a profound impact. Here, we review how chromatin modifications change the expression of genes whose impaired function is directly related to EC etiopathogenesis. Chromatin modification plays a central role in EC. The modification of chromatin structure alters the accessibility of genes to transcription factors and other regulatory proteins, thus altering the intracellular protein amount. Thus, DNA structural alterations may impair gene function as profoundly as mutations in the coding sequences. Hence, its central importance is in the diagnostic and prognostic evaluation of EC patients, with the caveat that chromatin alteration is often difficult to identify and needs investigations that are specific and not broadly used in common clinical practice. The different phases of the healthy endometrium menstrual cycle are characterized by differential gene expression, which, in turn, is also regulated through epigenetic mechanisms involving DNA methylation, histone post-translational modifications, and non-coding RNA action. From a medicolegal and policy-making perspective, the implications of using epigenetics in cancer care are briefly explored as well. Epigenetics in endometrial cancer is not only a topic of biomedical interest but also a crossroads between science, ethics, law, and public health, requiring integrated approaches and careful regulation. Full article

Fish production faces various challenges throughout its cycle, from rearing to consumption. Organic acids have emerged as an effective fish feed and meat treatment solution. They promote health and well-being, control pathogens, improve digestion, and contribute to food preservation. This study was therefore carried out to evaluate the evolution of publications on the use of organic acids in aquaculture over time, identifying the leading journals, authors, countries, and relevant organizations associated with the publications and determining the keywords most used in publications and research trends on this type of accommodation using bibliometric analysis. For this analysis, the Web of Science (WoS) and Scopus databases were used, with the keywords and Boolean operators “organic acid*” AND (“pathogens” OR “microorganism*” OR “bacteria” OR “fungi”) AND (“fish” OR “fry” OR “pisciculture”). Ninety-six articles were found in 44 journals, with the participation of 426 authors and 188 institutions, from 1995 to 2024. The most crucial publication source with the highest impact factor was the journal Aquaculture, with 14 articles, 2 of which were written by the most relevant author, Koh C., who received the highest number of citations and had the highest impact factor among the 426 authors. China had the most scientific production, with 26 publications on organic acids in aquaculture. However, Malaysia was the country that published the most cited documents, a total of 386. The most relevant affiliation was the University of Sains Malaysia, which participated in the publication of eight articles. The 10 most frequent keywords were fish, organic acids, citric acid, article, bacteria, growth, microorganisms, Oncorhynchus mykiss, animals, and digestibility. The results indicate increased publications on the benefits of using organic acids in aquaculture, highlighting their effectiveness as antibacterial agents and promoters of zootechnical development. However, gaps still require more in-depth research into the ideal dosages, mechanisms of action, and long-term impacts of these compounds. Full article

Hazards stemming from extreme natural events have exhibited heightened prominence in recent years. The natural hazard management process adopts a comprehensive approach that encompasses all stakeholders involved in the disaster management cycle. “Actions for Resilience” (A4R) represents a standardised concept derived from ISO/TR 22370:2020 that integrates principles from various scientific disciplines to enhance resilience in systems, whether they are socio-ecological systems, communities, or organisations. A4R emphasises proactive measures and interventions aimed at fostering resilience rather than merely reacting to crises or disruptions. It recognises that resilience is a multifaceted concept influenced by various factors, including social, economic, environmental, and institutional dimensions. Central to A4R is the understanding of complex system dynamics. Also, A4R involves rigorous risk assessment to identify potential threats and vulnerabilities within a system, as well as to build adaptive capacity within systems. A4R advocates for the development of resilience metrics and monitoring systems to assess the effectiveness of interventions and track changes in resilience over time. These metrics may include indicators related to social cohesion, ecosystem health, economic stability, and public infrastructure resilience. In this context, the study aims to apply the proposed hierarchy of factors and group decision-making using fuzzy numbers to identify strategic priorities for improving the urban resilience of the pilot area. The identified priority factors are then analysed across different scenarios, and corresponding actions are described in detail. Full article

Herpes simplex virus (HSV) is a prevalent and persistent human pathogen belonging to the family Herpesviridae and classified as an alpha-herpesvirus. It comprises two distinct types, HSV-1 and HSV-2, which together infect a significant portion of the global population and pose substantial public health challenges. HSV-1 is typically associated with oral herpes, while HSV-2 primarily causes genital herpes; both are characterized by recurrent lesions, latent infection, and mucocutaneous discomfort. Conventional antiviral drugs such as acyclovir and its derivatives are limited by drug resistance, potential toxicity, and their inability to eradicate latent viral reservoirs. These limitations have prompted increasing interest in alternative therapeutic strategies. Phenolic acids and tannins, plant-derived polyphenolic compounds, have attracted considerable attention due to their potent antiviral properties against various viruses, including HSV. This review summarizes current research on phenolic acids and tannins as promising natural antivirals against HSV, with a focus on their mechanisms of action and efficacy in disrupting multiple stages of the HSV life cycle. Full article

The influenza A virus (IAV) is a major cause of recurrent seasonal epidemics and global pandemics, posing a significant threat to public health. Although lycorine has demonstrated broad-spectrum antiviral activity, its specific mechanisms of action against IAV remain incompletely understood. In this study, we characterized the potent inhibitory effects of lycorine on seasonal and drug-resistant IAV subtypes (H1N1/H3N2) as well as the influenza B virus, showing its ability to suppress viral mRNA, viral titers, and M2 protein expression across multiple cell lines. Time-of-addition and time-course assays revealed that lycorine exerts multiphasic interference, and the critical late stage of the IAV life cycle aroused our interest to study this further. Mechanistically, we discovered that lycorine specifically interferes with the de novo synthesis of nucleoporin Nup93, thereby disrupting the nuclear export of viral nucleoprotein (NP). These findings not only establish lycorine as a promising broad-spectrum anti-influenza candidate but also provide new insights for developing host-targeted antiviral strategies. Full article

Introduction: Access to vaccines provided by the Brazilian National Immunization Program (NIP) to populations living in floodplains and hard-to-reach areas of the Amazon is complex and conditioned by the geographic characteristics of the region. The success of vaccination campaigns requires different strategies, technologies, and the involvement of professionals whose work goes beyond standard procedures and vaccination protocols. Objectives: To investigate the specificities of the immunization process of populations inhabiting floodplains and areas of difficult access in the municipality of Careiro da Várzea, in the state of Amazonas, Brazil. To analyze the theoretical and practical aspects of the National Immunization Program in the region. Methods: The case study included qualitative-descriptive techniques that combined data analysis, document analysis, and participant observation to reveal different socio-sanitary aspects of the immunization process of the Amazonian populations studied. The concepts of Street Level Bureaucracy and Social Technologies guided the analysis and description of the immunization process in the area studied. Results: The study described the geographic conditions, the social technologies used, and the individuals involved in the immunization process of the populations of communities and villages in flooded areas of the Amazon in Brazil. The high temperatures in the region create the need for thermal control in the storage of vaccines during their transfer to the communities and villages. The local coordination of the Imuniza SUS Program acts as a strategic mediator between the different bodies, ensuring the population’s access to vaccines, which means that the actions of government agents (Street Level Bureaucracy) are crucial to the functioning of the immunization program. Conclusions: The success of the immunization campaigns in the hard-to-reach flooded areas of the municipality is due to the existence of a virtuous cycle arising from the synergy between the different stakeholders that make up the immunization service; there is a clear relationship between the vaccination coverage rates achieved and the municipal administration’s commitment to public health. The immunization rates achieved in the municipality studied were compatible with the average established by the Brazilian Ministry of Health. This case study might enhance knowledge about health practices in this important world region. Full article

Prolonged exposure to public health crises in the post-pandemic era poses significant threats to global mental health. To address this, we developed a conceptual model to analyse the spatiotemporal distribution of public emotions, using Weibo data from the 2022 Beijing bar outbreak (9 June–18 August). The model integrates lexicon-based emotion analysis, spatial autocorrelation tests, and content analysis to provide a comprehensive understanding of emotional responses across stages and regions. The findings reveal a multi-peak emotional cycle spanning emergency, contagion, and resolution stages, with significant emotional clustering in emergency zones, surrounding areas, and regions visited by infected individuals. Through coding, we identified 24 main-categories and 90 sub-categories, distilled into nine core themes that illustrate the interplay between influencing factors, public emotions, and online behaviours. Positive public emotions (e.g., hopefulness, gratitude, optimism) were linked to pandemic improvements and policy implementation, driving behaviours such as supporting prevention measures and resisting misinformation. Negative emotions (e.g., anger, anxiety, sadness) stemmed from severe outbreaks, insufficient controls, and restrictions on freedoms, leading to criticism and calls for accountability. This study bridges big data analytics with behavioural science, offering critical insights into evolving public emotions and behaviours. By highlighting spatiotemporal patterns and emotional dynamics, it provides actionable guidance for governments and health organizations to design targeted interventions, foster resilience, and better manage future social crises with precision and empathy. Full article

Background: A Mediterranean-style dietary pattern (MDP) is embedded across coronary heart disease (CHD) and type 2 diabetes (T2D) clinical guidelines. However, MDP evidence has not consistently been translated into practice. This study aimed to develop, integrate and evaluate implementation strategies to support clinicians in translating MDP evidence into routine care for CHD and T2D in the local context of a public health service. Methods: This study documents the implementation and evaluation phases of a broader knowledge translation project guided by the Knowledge-to-Action cycle. Multi-disciplinary clinicians in the cardiology and diabetes services of two large metropolitan hospitals and a post-acute community service were targeted. Strategies were prioritised utilising theory and stakeholder engagement and included facilitation, building a coalition, the engagement of clinical champions and local opinion leaders, educational meetings, consensus discussions, sharing local knowledge, consumer consultation, and the development and distribution of education materials. Surveys were conducted with clinicians and patients of targeted services to evaluate the reach, acceptability, feasibility, adoption and perceived sustainability of MDP in practice. Results: In total, 57 clinicians (7 dietitians, 29 nurses/diabetes educators, 15 doctors and 6 other allied health professionals) and 55 patients completed post-implementation evaluation surveys. The majority of clinicians agreed an MDP is appropriate to recommend in their clinical setting (95%), and most of the time/always their advice (85%) aligns. Education sessions were attended by 65% of clinicians, of which the majority indicated improved knowledge (100%) and change in practice (86%). Factors deemed most important to maintaining an MDP approach in practice were hard-copy education materials (85%) and access to a dietitian (62%). Of the patients who had received care from a dietitian of targeted services (n = 32, 58%), 100% recalled having discussed ≥1 MDP topic and 89% received education material. Of the patients who had received dietary advice from non-dietetic clinicians (n = 33, 60%), 67% recalled having discussed ≥1 MDP topic and 70% received education material. Conclusions: Targeted and theory-informed implementation reached the majority of surveyed clinicians and patients, and positively influenced the adoption, acceptability and feasibility of an MDP approach in routine care. Ongoing sustainability strategies are crucial with rotating clinician roles. Full article

Climate change is a major global issue because transportation is a major source of pollutants and greenhouse gases that affect human health and air quality. However, to effectively prioritize and fund mitigating actions, decision-makers lack scientific rigor and diagnoses with sufficient spatial resolution. Based on the Origin-Destination Matrix (ODM), this study suggests a methodology to measure and identify mobility emissions (CO₂, Nox, PM) at the neighborhood level with high spatial resolution. Testing of the methodology was performed in Valencia, Spain. Even though many studies calculate carbon footprint, few make use of precise geographic information and openly accessible data, and they frequently concentrate on entire cities rather than smaller areas. To determine all potential routes for each Origin-Destination (OD) trip, the process uses geostatistics to estimate daily trip activity data (kilometers traveled). The COPERT calculator methodology from the European Union is used to analyze these routes to calculate the total emissions and the distance traveled per neighborhood. Based on road infrastructure, the methodology determines which neighborhoods receive emissions and creates measures of equitable environmental responsibility. It also identifies short trips that might be replaced by cycling or walking, as well as possible improvements to public transportation. Full article

Zero-emission buildings, which do not emit CO₂ or other greenhouse gases throughout their entire life cycle, play a crucial role in sustainable development and the fight against climate change. Achieving carbon neutrality in construction requires considering emissions associated with material production, construction, operation, as well as demolition and disposal. These buildings utilize energy-efficient technologies, renewable energy sources, and low-carbon materials, minimizing their environmental impact. The building sector accounts for a significant percentage of global greenhouse gas emissions, making it a key area for climate action. In Poland, where aging and energy-inefficient buildings prevail, the need for a transition towards zero-emission buildings is particularly urgent. This paper assesses the feasibility and hurdles of retrofitting existing buildings to achieve zero emissions by utilizing renewable energy systems like solar photovoltaic and heat pump technologies. The publication discusses the technical, economic, and legal aspects of this transformation, with particular emphasis on the Polish context and available support programs. The purpose of this publication is to disseminate practical knowledge and foster innovation among architects, investors, and decision-makers engaged in the development of a sustainable built environment. A key example is Net Zero Energy Buildings (NZEBs), which generate as much energy as they consume over a year through technologies such as photovoltaic panels, solar collectors, and heat pumps. NZEBs combine effective insulation, energy-efficient systems, and smart energy management to minimize consumption, and may even produce excess energy that feeds back into the grid. Despite challenges in construction and maintenance, the increasing adoption of zero-emission and NZEBs worldwide reflects their long-term ecological, economic, and health benefits. The focus of this publication is to analyze the potential for transforming standard buildings, as defined by current regulations, into zero-emission buildings powered entirely by renewable energy sources. This case study analyzes the energy potential of a residential building located in Krakow, Poland. The building’s energy efficiency potential was assessed through computer simulations using Audytor OZC software (version 7.0 Pro, Sankom), taking into account local climate conditions and building standards. The study analyzed the impact of various strategies, such as upgrading thermal insulation, using energy-efficient windows, and installing photovoltaic panels, on energy consumption and CO₂ emissions. Full article

Although the disease caused by chikungunya virus (CHIKV) is of great interest to public health organizations around the world, there are still no authorized antivirals for its treatment. Previously, dihalogenated anti-CHIKV compounds derived from L-tyrosine (dH-Y) were identified as being effective against in vitro infection by this virus, so the objective of this study was to determine the mechanisms of its antiviral action. Six dH-Y compounds (C1 to C6) dihalogenated with bromine or chlorine and modified in their amino groups were evaluated by different in vitro antiviral strategies and in silico tools. When the cells were exposed before infection, all compounds decreased the expression of viral proteins; only C4, C5 and C6 inhibited the genome; and C1, C2 and C3 inhibited infectious viral particles (IVPs). Furthermore, C1 and C3 reduce adhesion, while C2 and C3 reduce internalization, which could be related to the in silico interaction with the fusion peptide of the E1 viral protein. Only C3, C4, C5 and C6 inhibited IVPs when the cells were exposed after infection, and their effect occurred in late stages after viral translation and replication, such as assembly, and not during budding. In summary, the structural changes of these compounds determine their mechanism of action. Additionally, C3 was the only compound that inhibited CHIKV infection at different stages of the replicative cycle, making it a compound of interest for conversion as a potential drug. Full article

Influenza A virus (IAV) continues to pose serious threats to the global animal industry and public health security. Identification of critical host factors engaged in the life cycle of IAV and elucidation of the underlying mechanisms of their action are particularly important for the discovery of potential new targets for the development of anti-influenza drugs. Herein, we identified Hydroxyacyl-CoA Dehydratase 3 (HACD3) as a new host factor that supports the replication of IAV. Downregulating the expression of HACD3 reduced the level of viral PB1 protein in IAV-infected cells and in cells that were transiently transfected to express PB1. Silencing HACD3 expression had no effect on the level of PB1 mRNA but could promote the lysosome-mediated autophagic degradation of PB1 protein. Further investigation revealed that HACD3 interacted with PB1 and selective autophagic receptor SQSTM1/p62, and HACD3 competed with SQSTM1/p62 for the interaction with PB1, which prevented PB1 from SQSTM1/p62-mediated autophagic degradation. Collectively, these findings establish that HACD3 plays a positive regulatory role in IAV replication by stabilizing the viral PB1 protein. Full article

The field of technology assessment in telemedicine is garnering increasing attention due to the widespread adoption of this discipline and its complex and heterogeneous system characteristics, making its application complex. As part of a national telemedicine project, the National Center for Innovative Technologies in Public Health at the Italian National Institute of Health played the role of promoting and utilizing technology assessment tools within partnership projects. This study aims to outline the design, development, and application of assessment methodologies within the telemedicine project proposed by the ISS team, utilizing a specific framework developed within the project. The sub-objectives include evaluating the proposed methodology’s effectiveness and feasibility, gathering feedback for improvement, and assessing its impact on various project components. The study emphasizes the multifaceted nature of action domains and underscores the crucial role of technology assessments in telemedicine, highlighting its impact across diverse realms through iterative interaction cycles with project partners. Both the impact and the acceptance of the methodology have been assessed by means of specific computer-aided web interviewing (CAWI) tools. The proposed methodology received significant acceptance, providing valuable insights for refining future frameworks. The impact assessment revealed a consistent quality improvement trend in the project’s products, evident in methodological consolidations. The overall message encourages similar initiatives in this domain, shedding light on the intricacies of technology assessment implementation. In conclusion, the study serves as a comprehensive outcome of the national telemedicine project, witnessing the success and adaptability of the technology assessment methodology and advocating for further exploration and implementation in analogous contexts. Full article

Background: The global COVID-19 pandemic has profoundly affected public health. Directly, the pandemic resulted in over 6.6 million deaths, numerous hospitalizations, and widespread illness. The pandemic has also affected health indirectly through government-imposed protective measures, causing decline in mental well-being and increasing social isolation. Unlike previous disasters or crises, the pandemic’s worldwide and enduring impact necessitates a unique research approach. The Network for Health Research in Disasters in the Netherlands responded by initiating a longitudinal, extensive research project called the Integrated Health Monitor COVID-19. The Integrated Health Monitor COVID-19 explores both the direct and indirect health effects of the pandemic at the population level. Methods: The Integrated Health Monitor COVID-19 employs a dual-pronged monitoring strategy alongside an annual literature review. This strategy comprises short-cycle monitoring (conducted quarterly) and long-cycle monitoring (conducted once every one or two years). This comprehensive approach enables the evaluation of health trends during the pandemic, facilitating comparisons with pre-pandemic levels and identification of risk and protective factors. Both monitoring methods incorporate data from surveys and general practice registries. The integration of annual literature reviews with these measurements enables iterative research, while dialogues on policy and practice improvements enhance the knowledge-to-action process. Discussion: Much of the existing knowledge about the potential impact of the COVID-19 pandemic is derived from research on sudden-onset disasters limited to specific geographical areas. This study is anticipated to provide valuable fresh insights into the evolving dynamics of population health and specific vulnerabilities within the ongoing pandemic context. Full article

Cancer is a major public health concern worldwide in terms of mortality. The exact reason behind the development of cancer is not understood clearly, but it is evidenced that alcohol consumption, radiation, and exposure to chemicals are main players in this pathogenesis. The current mode of treatments such as surgery, chemotherapy, and radiotherapy are effective, but, still, cancer is a major problem leading to death and other side effects. However, safer and effective treatment modules are needed to overcome the adverse effects of current treatment modules. In this regard, natural compounds have been recognized to ameliorate diseases by exerting anti-inflammatory, anti-oxidative, and anti-tumor potential through several mechanisms. Mangiferin, a xanthone C-glucoside, is found in several plant species including Mangifera indica (mango), and its role in disease prevention has been confirmed through its antioxidant and anti-inflammatory properties. Furthermore, its anti-cancer-potential mechanism has been designated through modulation of cell signaling pathways such as inflammation, angiogenesis, PI3K/AKT, apoptosis, and cell cycle. This article extensively reviews the anticancer potential of mangiferin in different cancers through the modulation of cell signaling pathways. Moreover, the synergistic effects of this compound with some commonly used anti-cancer drugs against different cancer cells are discussed. More clinical trials should be performed to reconnoiter the anti-cancer potential of this compound in human cancer treatment. Further, understanding of mechanisms of action and the safety level of this compound can help to manage diseases, including cancer. Full article