Search Results (6)

Lap joining of an aluminum AA6082-T6 plate and a UHSS steel plate coated with an Al-Si layer was performed using Probeless Friction Stir Spot Welding (P-FSSW). The dwell time and rotational speed were controlled in the range of 10–15 s and 1000–1500 rpm, respectively. For all the samples, thermo-mechanical deformation occurred solely within the upper AA6082 plate. A refined grain structure was formed in the aluminum plate close to the surface. The dwell time was responsible for the intensity of the material flow, resulting in stirring between the Al-Si layer and the aluminum plate at 15 s. The microhardness distribution corresponded to the microstructure features. Full article

Electrochemical impedance spectroscopy (EIS) is a sensitive analytical method for surface and bulk properties. Classical EIS and derived electrochemical capacitance spectroscopy (ECS) with a redox couple are label-free approaches for biosensor development, but doubts arise regarding interpretability when a redox couple is employed (redox EIS) due to interactions between electroactive probes and interfacial charges or forced potential. Here, we demonstrated redoxless ECS for directly determining surfactant adsorption on screen-printed carbon electrodes (SPCEs), validated through a simulation of equivalent circuits and the electrochemistry of electronic dummy cells. Redoxless ECS provides excellent capacitance plot loci for quantifying the interfacial permittivity of di-electric layers on electrode surfaces. Redoxless ECS was compared with redox EIS/ECS, revealing a favorable discrimination of interfacial capacitances under both low and high SDS coverage on SPCEs and demonstrating potential for probeless (reagentless) sensing. Furthermore, the proposed method was applied in an electrolyte without a redox couple and bare electrodes, obtaining a high performance for the adsorption of surfactants Tween-20, Triton-X100, sodium dodecyl sulfate, and tetrapropylammonium bromide. This approach offers a simple and straightforward means for a semi-quantitative evaluation of small molecule interactions with electrode surfaces. Our proposed approach may serve as a starting point for future probeless (reagentless) and label-free biosensors based on electrochemistry, eliminating disturbance with surface charge properties and minimizing forced potential bias by avoiding redox couples. An unambiguous and quantitative determination of physicochemical properties of biochemically recognizable layers will be relevant for biosensor development. Full article

In the present study, AA2024 aluminum alloy and AISI304 stainless steel were welded in a lap joint configuration by Probeless Friction Stir Spot Welding (P-FSSW) with a flat surface tool. A full factorial DOE plan was performed. The effect of the tool force (4900, 7350 N) and rotational speed (500, 1000, 1500, 2000 RPM) was analyzed regarding the microstructure and microhardness study. A two-dimensional arbitrary Eulerian–Lagrangian FEM model was used to clarify the temperature distribution and material flow within the welds. The experimental results for the weld microstructures were used to validate the temperature field of the numerical model. The results showed that the tool rotation speed had an extensive influence on the heat generation, whereas the load force mainly acted on the material flow. Full article

Zika virus (ZIKV) is a mosquito-borne infection, predominant in tropical and subtropical regions causing international concern due to the ZIKV disease having been associated with congenital disabilities, especially microcephaly and other congenital abnormalities in the fetus and newborns. Development of strategies that minimize the devastating impact by monitoring and preventing ZIKV transmission through sexual intercourse, especially in pregnant women, since no vaccine is yet available for the prevention or treatment, is critically important. ZIKV infection is generally asymptomatic and cross-reactivity with dengue virus (DENV) is a global concern. An innovative screen-printed electrode (SPE) was developed for amperometric detection of the non-structural protein (NS2B) of ZIKV by exploring the intrinsic redox catalytic activity of Prussian blue (PB), incorporated into a carbon nanotube–polypyrrole composite. Thus, this immunosensor has the advantage of electrochemical detection without adding any redox-probe solution (probe-less detection), allowing a point-of-care diagnosis. It was responsive to serum samples of only ZIKV positive patients and non-responsive to negative ZIKV patients, even if the sample was DENV positive, indicating a possible differential diagnosis between them by NS2B. All samples used here were confirmed by CDC protocols, and immunosensor responses were also checked in the supernatant of C6/36 and in Vero cell cultures infected with ZIKV. Full article

In this work, three types of probeless tools (archimedes, involute, and concave tools) were designed. A 7075-T651 sheet of 1.0 mm thickness was welded using the designed probeless tools. It was found that the stir zone and hook defect varied a lot for different joints. Plunge depth was the dominant process parameter for joint property. The joint’s maximum failure load (5.73 kN) was obtained with the concave tool when the target plunge depth was 0.55 mm. Two typical joint fracture modes (shear fracture and plug fracture) were found using three designed tools with different plunge depths, rotation speeds, and dwell times. Shoulder geometry presented little influence on heat generation. Compared with involute grooves, archimedes grooves showed to be more effective on the material flow. The properties of the joints with the archimedes tool were more sensitive to welding parameters. Compared with the flat tool, the concave tool decreased the escape of plasticized material, improved the forge force, and optimized the metallurgical bond at the interface. Full article

Amyloid beta (aβ) 1-42, a peptide that is 1-42 amino acids long, is a major component of senile plaques in the brains of patients with Alzheimer’s disease. Aβ detection has become an essential antecedence to predict the declining mental abilities of patients. In this paper, a probeless capacitive biosensor for the non-Faradaic detection of aβ 1-42 peptide was developed by immobilizing a specific anti-aβ antibody onto a self-assembled monolayer functionalized interdigitated chain-shaped electrode (anti-aβ/SAM/ICE). The novelty and difference of this article from previous studies is the direct detection of aβ peptide with no redox probe ((Fe(CN)₆)^3−/4−), which can avoid the denaturation of the protein caused by the metallization (binding of aβ to metal ion Fe which is presented in the redox couple). The direct detection of aβ with no redox probe is performed by non-Faradaic capacitive measurement, which is greatly different from the Faradaic measurement of the charge transfer resistance of the redox probe. The detection of various aβ 1-42 peptide concentrations in human serum (HS) was performed by measuring the relative change in electrode interfacial capacitance due to the specific antibody-aβ binding. Capacitance change in the anti-aβ/SAM/ICE biosensor showed a linear detection range between 10 pg mL⁻¹ and 10⁴ pg mL⁻¹, and a detection limit of 7.5 pg mL⁻¹ in HS, which was much lower than the limit of detection for CSF aβ 1-42 (~500 pg mL⁻¹) and other biosensors. The small dissociation constant K_d of the antibody-antigen interaction was also found to be 0.016 nM in HS, indicating the high binding affinity of the anti-aβ/SAM/ICE biosensor in the recognizing of aβ 1-42. Thus, the developed sensor can be used for label-free and direct measurement of aβ 1-42 peptide and for point-of-care diagnosis of Alzheimer’s disease without redox probe. Full article