Search Results (21)

Osteoarthritis (OA) is a chronically progressive degenerative arthropathy characterized by the loss of cartilage, changes in subchondral architecture, and ongoing inflammation resulting in reduced mobility and pain. This study assessed the treatment potential of a combination of chondroitin and glucosamine enriched with Curcumin C3 Complex (C3GC) in modulating the pathophysiological features in mouse models with surgically induced OA and in dogs with naturally occurring OA. A cohort of 24 male C57BL/6 mice aged 3 months old were surgically destabilized with medial meniscus (DMM) to cause osteoarthritis. These animals underwent a nutritional intervention with C3GC or with GC over a course of 8 weeks. In order to evaluate cartilage health and subchondral bone structure, we carried out a combination of behavioral tests, micro-computed tomography (micro-CT), and histopathological examinations. In addition, a cohort of 12 OA-diagnosed retired police dogs were administered C3GC supplements or conventional care over a course of 30 days, with pain measurement and serum concentrations of MMP-3 and TNF-α determined before and after treatment. According to our findings, the administration of C3GC was determined to preserve subchondral microarchitectural structure integrity (p < 0.05) and resulted in better motor function in comparison with GC. In animals taking nutritional supplements, the OARSI scores of joint tissue sections were reduced, with the medial tibial plateau OARSI score being particularly low in the C3GC group (p < 0.0001). In dogs, treatment with C3GC resulted in a 24.5% reduction in serum MMP-3 levels (p < 0.01), and there was also a 20.8% decrease in serum TNF-α levels (p < 0.05), along with a decrease in subjective pain assessment. The results are in support of the chondroprotective, anti-inflammatory, and analgesic properties of C3GC and justify future research on the potential utility of C3GC in treating osteoarthritis. Full article

Between September 2013 and 2022, the American Society for the Prevention of Cruelty to Animals (ASPCA) received 2783 suspected animal cruelty cases involving 5745 animals through a partnership with the New York City Police Department (NYPD). These cases involved dogs (2271/2783, 82%), cats (408/2783, 15%), and other species (104/2783, 4%). Dogs were most likely to be presented for suspected neglect (1424/2271, 63%), and cats for suspected non-accidental injury (233/408, 58%). Animals were most often presented by law enforcement (1018/2783, 37%), municipal shelters (383/2783, 14%), and veterinarians (311/2783, 11%). These findings contribute to understanding neglect as a commonly reported type of animal cruelty. Cruelty toward cats appears less well understood and may be under-reported or more severe, requiring further research and attention. Full article

Working dogs are an essential part of modern society, and police canines (K9s) in the United States are amongst the most recognizable of all working dogs. Given the dearth of published data on active police canines in the U.S. and the calls for police reform, an interdisciplinary effort is necessary to comprehensively understand how these dogs are best used for the benefit of society. This review paper relies on veterinary public health expertise to present a comprehensive overview of police canine use by municipal law enforcement in the United States, including known impacts and gaps in knowledge. The existing literature from animal-assisted interventions (AAI) provides evidence to ensure working dog well-being, while human–animal bond research contributes to officer safety and canine partnership. Lastly, law enforcement and public health agendas (LEPH) assure the team’s successful efforts in built environments and local neighborhoods. These frameworks acknowledge the complexity of police dog use, spanning from punitive to public relations, which mirrors the role of law enforcement in U.S. society. This paper proposes the use of a One Health framework to ensure police canine contributions to society, including suggested approaches to partner municipal police with veterinary medicine and public health, and integrate One Health in promoting public safety for local communities. Full article

The aim of the study was to point out the importance of recognizing non-accidental injuries (NAI) and to highlight the importance of reporting such cases, as well as prosecuting the perpetrators, in order to detect potentially existing violence or prevent future violence in society. A total of 338 dogs and 54 cats were submitted for necropsy. Out of 338 dogs examined, 175 (51.8%) died due to non-natural cause of death, 122 from natural causes (36.1%), and 35 (10.3%) due to accidental injuries, and in 6 cases (1.8%) the cause and manner of death were undetermined due to advanced post-mortal changes. Out of 54 examined cats, 21 died due to non-natural causes (38.9%) and the same number of cats died due to natural causes. The prevalence of accidental injuries resulting in death were 20.4% (n = 11) and in 1.8% (n = 1) the manner of death remained indeterminate. The high number of animals confirmed to have died from unnatural causes in this study highlights the need for greater involvement from the police, prosecution, and society as a whole to reduce the number of violent animal deaths in the future. Full article

Background: Although the Coxiella burnetii infection has been investigated in dogs, its role in human transmission remains to be fully established, particularly in close and daily human–dog contact settings, such as in Police K-9 Units. Methods: Accordingly, this study aimed to assess anti-C. burnetii antibodies in clinically healthy police officers by an in-house indirect immunofluorescence assay (IFA), and working dogs by a commercial IFA Kit, from the State Special Operations Battalion, Paraná, Southern Brazil. Results: Overall, 1/18 (5.5%) police officers and 9/30 (30.0%; CI 95% 16.66–47.88) dogs tested seropositive to anti-C. burnetii IgG antibodies. Conclusions: To date, this is the highest prevalence of Q fever seropositivity among military dogs worldwide. Despite the low sampling rate, a statistically significant association was found between seropositivity and female dogs (p = 0.0492). Further studies with larger sample sizes should be conducted to establish the prevalence of Q Fever in other Brazilian K-9 Units. In summary, this study is the first to conduct a concomitant serosurvey of police officers and working dogs, and its findings should be considered a warning for cross-exposure and transmission of Coxiella burnetii among Police K-9 Units in Brazil and worldwide. Full article

This study examined the percentage and location of trigger points in police working dogs. Twelve dogs housed at a military police kennel were selected through convenience sampling. Only active dogs with no comorbidities or radiographic changes doing 6 hours of intense physical activity per day were included. After orthopedic and neurological examination, dogs were palpated for the detection of trigger points (TPs), carried out by two independent examiners, with criteria of palpations previously standardized. TPs were recorded using an anatomy reference image according to the corresponding anatomical location. The percentage of TPs was highest in the lumbar portion of the longissimus dorsi muscle (42%), followed by the latissimus dorsi, pectineus, quadriceps femoris, and sartorius (33%) muscles. Most TPs were located on the right side of the body. This study’s percentage of TPs in police working dogs was higher in spinal and hind limb muscles, especially on the right side. The major criteria for identifying TPs in dogs were the pain responses to palpation and contractile local response. The findings of this study could be used to refine myofascial pain prevention to reduce early retirement due to musculoskeletal pain and draw attention to this kind of problem that can also affect dogs. Full article

Osteoarthritis (OA) is the most commonly diagnosed joint disease in companion animals, and proper tools are necessary to assess patients and response to treatment. We aimed to perform the psychometric evaluation of several clinical metrology instruments (CMI), developed to evaluate pain and assess outcome. Fifty police working dogs with bilateral hip OA were assessed in a prospective, randomised, double-blinded study. Patients were evaluated using a stance analyser in six different moments divided over a 180-day period. Pedometer step count, weight-bearing symmetry index and deviation from normal weight-bearing were calculated and used for criterion validity. In each evaluation moment, a copy of the Hudson Visual Analogue Scale (HVAS), Canine Brief Pain Inventory (CBPI), Liverpool Osteoarthritis in Dogs (LOAD) and Canine Orthopaedic Index (COI) were completed by the dogs’ handlers. Correlations between CMIs were evaluated as construct validity. Further evaluation was performed with the Kaiser–Meyer–Olin measure of sampling adequacy, Eigenvalue and scree-plot analysis. Internal consistency was tested with Cronbach’s α. Significant weak correlation was found between all CMIs and stance analysis symmetry index measure and deviation, indicating criterion validity. Significant weak correlation was also found between pedometer count and LOAD plus COI. Cronbach’s α was 0.80 for HVAS, 0.98 for CBPI, 0.97 for LOAD and 0.98 for COI. Significant strong correlation was observed between CMIs, indicating construct validity. We present criterion and construct validity of these CMIs, which are able to capture various dimensions of OA. They can be used for the evaluation of osteoarthritis and response to treatment in dogs. Full article

Nose work with military working dogs (MWDs) in warmer-than-usual areas has led us to look for new tools to reduce both heat stress and the risk of heat stroke. One of the different strategies to manage heat stress is the use of cooling vests, such as those used in humans. The aim was to assess three cooling conditions (using two different cooling vests during exercise and the non-use of such garments) by measuring core body temperature, systemic blood pressure and pulse rate before and after the exercise (moment: four measurement times) in military dogs of the I Military Police Battalion (in Valencia, Spain). All dogs were evaluated under all three conditions during the three days of the study. Significant differences were observed between condition, moment, and the interaction of these two factors, in relation to core body temperature and pulse rate. Therefore, the use of an evaporative cooling vest may further be useful as a routine thermal control and conditioning measure in MWDs. Full article

We report the case of a young man, a former heroin addict, found dead at home by the Police Forces in an advanced state of decomposition. Numerous blisters and unpacked tablets of medications were found all over the bed and on the floor of the room. Multiple injuries to the face, left arm and neck of the deceased were noted. The latter damages were attributed to post-mortem dog bites, since no indications of a possible defense against the animal were observed. The autopsy findings were unremarkable. Toxicological investigations performed on peripheral blood and urine by gas chromatography-mass spectrometry (GC-MS) technique showed the presence of acetaminophen, citalopram and trazodone. Combined drug intoxication was proposed as the cause of death since acetaminophen and trazodone concentrations were comparable with the ones found in fatal cases. Moreover, citalopram concentration in peripheral blood was above the toxic range and in accordance with levels found in fatalities due to poly-drug intoxication. Full article

The synthesis, degradation, and reconstruction of the cell membrane as a metabolic pathway of phospholipids is a constant and dynamic process. Fatty acids as bioactive lipid components of plasma and erythrocyte phospholipids as structural lipids have biological roles in the integrity of cell membranes. Fatty acids, depending on the chain length, the degree of saturation, and the synthesis pathways, can alleviate inflammation and oxidative stress caused by excessive exercise. Considering that changing food intake or diet can influence fatty acid phospholipid metabolism, our study aimed to determine the potential benefits of fish-based diets in working (police) dogs undergoing intensive training concerning bioactive lipids such as fatty acids, phospholipids of plasma, and erythrocytes. Fatty acid esters’ composition of plasma and erythrocyte phospholipids as a bioactive lipids, in addition to markers of oxidative stress and metabolic parameters, were analysed by GC chromatography. The food was well tolerated by all dogs, and the compliance to the diet was high throughout the study. After the treatment with fish-based food, blood glucose, total, and LDL cholesterol levels were significantly reduced, indicating positive biochemical profiles of dogs. Correlations of fatty acid phospholipid compositions between plasma and erythrocytes have shown that both plasma and erythrocytes could represent markers of omega-3 eicosapentaenoic and docosahexaenoic acid intake levels in dogs. Morover, fish-based food supplementation caused a significant reduction in lipid peroxidation markers. The enrichment of dogs’ diets with marine fish could improve oxidative status and improve roles and status of bioactive lipids, such as membrane phospholipids and fatty acids, as its components in plasma and erythrocytes in police dogs exposed to intensive exercise. Full article

Dogs are the most popular pets worldwide; however, close contact with people increases the risk of transmission of different zoonotic parasites. This study aims to determine the prevalence of gastrointestinal helminths in dogs in Slovakia. A total of 495 faecal samples collected from pet, shelter, guard, working (police), and hunting dogs, as well as dogs from segregated Roma settlements between 2016 and 2021, were examined using flotation and molecular methods. Eggs of intestinal helminths were detected in 134 (27.1%) samples. Microscopically, six different species/genera/families, namely, Toxocara canis (14.7%), Toxascaris leonina (1.6%), Trichuris vulpis (6.3%), Capillaria spp. (1.4%), Ancylostoma/Uncinaria spp. (8.3%), and taeniid eggs (4.0%), were recorded. Molecular analyses revealed infection with Echinococcus multilocularis in 2.2% of dogs and 0.4% of the animals were infected with Taenia hydatigena. The results showed a correlation between the occurrence of intestinal helminths and the availability of veterinary care, as dogs from Roma settlements and shelter dogs were the most often infected (66.7% and 39.2%, respectively). On the other hand, working animals were in the best health condition, with only 2.5% being positive. The relatively frequent occurrence of zoonotic species points to the constant need for preventive measures and regular deworming of dogs. Full article

Working trials is a competitive canine discipline based on work undertaken by military and police dogs. A 9 ft long jump is a key component of the discipline. Research into landing forces and joint angulation in other canine disciplines has highlighted the potential for the occurrence of soft tissue injuries, predominantly in the front limbs. There is a paucity of work into the impact of spread/long jumps on joint angulation and peak vertical force (PVF) on landing, and limited research on working trials dogs generally. This study aimed to determine whether altering the length of the long jump impacted PVF and apparent joint angulation upon landing. 21 dogs regularly competing in working trials cleared the long jump at three lengths: 9 ft (full length), 8 ft, and 7 ft. The impact of altered long jump length on the PVF, apparent shoulder and carpus angulation, and duration of landing, were analysed using general linear mixed models. There was no significant relationship between the length of the long jump and PVF or joint angulation on landing (p > 0.05). Greatest joint compression was observed on landing after clearing 9 ft. Individual variability in landing joint angulation, PVF and force distribution of the left and right front limbs on landing was observed across all three experimental lengths. We recommend further research is undertaken to examine individual variability and the effect of training and experience in working trials participants, to provide evidence-based recommendations for training people and competing dogs in this discipline. Full article

This work was aimed at identifying the effectiveness of two interventions applied to a group of eight kennelled police dogs. Interventions consisted of access to a lawn area (350 m²) and “toy” (a 30 cm jute rag roll, hanging from the kennel ceiling), both available for 15 min a day, for four days in a row. We collected behavioural data and faecal samples for cortisol metabolites evaluation before, during and after interventions. Faecal cortisol metabolites levels were significantly reduced (Friedman, X2(3) = 12.450; p = 0.006) during the second round of intervention, regardless of the type of intervention, indicating that the interventions can have a beneficial cumulative effect. Regarding the frequency of stereotyped behaviour, cluster analysis identified two groups of individuals: (1) high-stereotyping individuals (n = 3) that had a tendency to reduce stereotyping behaviours in the lawn intervention when compared to toy intervention (Friedman, X2(3) = 2.530; p = 0.068), and (2) low-stereotyping animals (n = 5) that did not present significant behavioural changes during the experiment. The variety of behavioural and endocrine parameters evaluated highlights the need to account for the individual in behaviour and welfare research. Overall, our results suggest that even simple environmental enrichment can be an effective method to mitigate behaviour and physiological signs of stress. Full article

Information on the biomechanics of police dogs’ tasks is important in understanding their work-related injuries and dysfunctions. This study aimed to develop and test a measurement tool for dogs’ functional bite force and to report modified long attack-related kinetic and kinematic values. Twenty Finnish male police dogs, 7 German Shepherd Dogs (GSDs) and 13 Belgian Shepherd Dogs, Malinois (BSDMs), were included. Dogs accelerated 25 m and bit the helper’s sleeve, fitted with three force sensors. Dogs were wearing a 3D accelerometer and were videotaped with a high-speed camera. The sleeve’s reliability for measuring the dog’s bite force was evaluated via intraclass correlation and Cronbach’s alpha. Otherwise, a Mann–Whitney U-test was used, with significance set at p = 0.05. The sleeve’s test-retest reliability was moderate to good (intraclass correlation of 0.75), and internal consistency was high (Cronbach’s alpha 0.75). The GSDs’ median bite force was 360.4 N (interquartile range (IQR) 628.6 N) and BSDMs’ 247.0 N (IQR 289.8 N). Median acceleration maximum was 7.1 gravitational force equivalent (g) and median deceleration maximum was 11.6 g, with highest recorded forces being 9.2 g and 13.1 g, respectively. The measurement sleeve was a reliable tool for measuring functional bite force in GSDs and BSDMs. Forces related to bite, approach and impact in the two breeds were reported. Full article

Previous histories of animal experimentation, e.g., Franco (2013) have focused on ethics, the law and the personalities involved, but not on the involvement of anaesthetics or analgesics. Given that these were major subjects of (UK) Parliamentary debates on vivisection in the mid-19th century and viewed as “indisputable refinements in animal experimentation” (Russell and Burch 1959), it seemed that an analysis of their role was overdue. This commentary has, in interweaving the history of animal experimentation in the UK with the evolution of anaesthesia, attempted to: (1) clarify the evidence for Russell and Burch’s view; and (2) evaluate anaesthesia’s ongoing contribution to experimental refinement. The history that emerges reveals that the withholding or misuse of anaesthetics and, or analgesics from laboratory animals in the UK has had a profound effect on scientists and indirectly on the attitudes of the British public in general, becoming a major driver for the establishment of the anti-vivisection movement and subsequently, the Cruelty to Animals Act (1876)—the world’s first legislation for the regulation of animal experimentation. In 1902, the mismanaged anaesthetic of a dog in the Department of Physiology, University College London resulted in numerous events of public disorder initiated by medical students against the police and a political coalition of anti-vivisectionists, trade unionists, socialists, Marxists, liberals and suffragettes. The importance of anaesthesia in animal experiments was sustained over the following 150 years as small mammalian species gradually replaced dogs and cats as the principle subjects for vivisection. In discussing experimental refinement in their 1959 report, “The Principles of Humane Experimental Technique” Russell and Burch described anaesthetics as “… the greatest single advance in humane technique, (which) has at the same time been virtually indispensable for the advance of experimental biology”. Since then, the role of anaesthetics and in particular analgesics has become an unavoidable consideration whenever animal experiments are planned and conducted. This has been accompanied by a proliferation of training and educational programmes in laboratory animal anaesthesia. Full article