Search Results (2,011)

Ruminal acidosis, particularly subacute ruminal acidosis, remains a major metabolic disorder that compromises animal health, production efficiency, and the sustainability of intensive ruminant systems. Although traditionally defined by reduced ruminal pH, it is increasingly recognized as a multidimensional disorder involving microbial ecological destabilization, disrupted metabolic cross-feeding, impaired epithelial barrier function, and dysregulated host inflammatory responses. This review synthesizes current knowledge of the microbial and metabolic mechanisms underlying acute and subacute ruminal acidosis and highlights processes that extend beyond pH depression alone. High-concentrate feeding shifts the balance among amylolytic and lactate-producing microorganisms, lactate-utilizing populations, and fibrolytic guilds, thereby promoting organic acid accumulation, reducing functional redundancy, and weakening microbial resilience. Concurrent increases in volatile fatty acids, lactate, lipopolysaccharide, histamine, and other microbially derived bioactive compounds increase epithelial acid load, disrupt tight-junction integrity, and facilitate inflammatory signaling. The principal novelty of this review is the integration of microbial functional guilds, metabolic cross-feeding, ecological resilience, epithelial barrier dysfunction, and host inflammation into a unified microbiota–metabolism–barrier–inflammation framework linking dietary perturbation with microbial dysfunction and host pathology. We further critically evaluate nutritional regulation, buffering agents, probiotics, yeast-derived products, postbiotics, and plant bioactive compounds according to their capacity to restore microbial function rather than merely correct ruminal pH. Additionally, this review may support multidimensional risk assessment, guide targeted intervention, and facilitate the integration of continuous ruminal monitoring with precision nutrition for earlier prediction and individualized prevention of ruminal acidosis. Full article

Herein, we report an unexpected pseudo-multicomponent transformation discovered during attempts to selectively nitrate the pyridine core of 3-acetyl-2,4,6-trimethylpyridine (3). Despite employing standard nitration conditions, including KNO₃–H₂SO₄ and HNO₃–H₂SO₄ mixtures, electrophilic substitution of the aromatic ring did not occur. Instead, the reaction sequence promoted an in situ nitrozation, dehydration to nitrile oxide intermediates, and subsequent [3+2]-cycloaddition involving two substrate molecules. This process yielded a novel, highly functionalized furoxan derivative, precisely identified as 3,4-bis(2,4,6-trimethylnicotinoyl)-1,2,5-oxadiazole 2-oxide (5). The molecular architecture of compound 5 was established by ¹H and ¹³C NMR spectroscopy, mass spectrometry, elemental analysis, and single-crystal X-ray diffraction (XRD) analysis. To elucidate the stereochemical and electronic features governing compound 5, DFT calculations were performed at the ωB97X-D/6-311++G(d,p) level of theory. The experimental crystallographic disorder of the N-oxide oxygen atom was computationally rationalized by the thermodynamic near-degeneracy (ΔG < 0.63 kcal/mol) of two orientational isomers (5a and 5b). Furthermore, frontier molecular orbital analysis within the framework of perturbation theory accounted for the head-to-tail regioselectivity during cyclization, while wide energy gaps (ΔE = 8.13–8.27 eV) and high chemical hardness (η = 4.07–4.14 eV) underscored the kinetic stability of the heterocycle. Phenotypic and target-specific in silico profiling using PASS Online identified Matrix Metalloproteinase-9 (MMP-9) as a relevant target for potential hemorheological and cardioprotective applications. Validated molecular docking simulations across three human MMP-9 crystallographic domains (PDB: 8K5Y, 6ESM, 4XCT) demonstrated competitive binding affinities and balanced Ligand Efficiency metrics (LE = 0.26–0.29 kcal/mol/heavy atom), anchoring compound 5 within the catalytic pocket via conventional hydrogen bonds and π-mediated interactions. Finally, in vitro evaluations using a blood hyperviscosity model confirmed significant hemorheological efficacy, as compound 5 effectively prevented the rise in blood viscosity, outperforming the reference drug pentoxifylline. The convergence of computational insights and experimental functional activity establishes this novel bis(nicotinoyl)furoxan framework as a promising candidate for further hemorheological and cardioprotective applications. Full article

Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder involving metabolic impairment, neuroinflammation, synaptic failure, and comorbidities. Hence, therapeutic development for AD is rapidly shifting from a single-target approach, centred on amyloid-beta (Aβ) reduction, to multi-target strategies. In this study, we investigated the neuroprotective profile of two acetanilide derivatives, SG-22 and SG-23, originated from the halogen-free thyronamine-like lead compound SG-2. Their efficacy was evaluated through an integrated approach combining in vitro cellular models, in vivo phenotypic screening in a Caenorhabditis elegans AD model, and comprehensive ADME-Tox profiling. In U87MG cells, both SG-22 and SG-23 effectively prevented Aβ_25–35-induced cytotoxicity and restored autophagy-related gene expression, including LC3, SIRT1, and SIRT6, while reducing mTOR and SIRT5 levels. Furthermore, all compounds exhibited anti-inflammatory effects in activated HMC3 microglial cells, reducing IL-6 and increasing IL-10 levels, with evidence suggesting partial involvement of TAAR1 signalling. ADME-Tox analyses revealed improved safety and metabolic profiles for the tested compounds, particularly SG-22, which showed reduced hERG liability and enhanced cytochrome P450 stability. However, in vivo studies demonstrated that only SG-2 and SG-23 improved motility and fitness in the C. elegans AD model, consistent with their ability to activate autophagy, whereas SG-22 was ineffective due to limited organismal uptake. Ultimately, the monoacetylated analogue SG-23 emerges as a promising candidate, balancing neuroprotective efficacy and drug-like properties, and supporting thyronamine-like analogues as multi-target agents for AD. Full article

Background: Coagulopathy in sepsis represents a dynamic continuum ranging from sepsis-induced coagulopathy (SIC) to disseminated intravascular coagulation (DIC), but its early evolution and clinical determinants remain poorly defined. Objectives: To assess the temporal dynamics of SIC and DIC and examine factors associated with their evolution during the early phases of sepsis. Methods: A prospective longitudinal observational study was conducted on 299 patients with sepsis admitted to the Intermediate Care Unit (IMCU). Patients were evaluated at admission and subsequently at 24, 48, 72, and 96 h, for a total of 1.447 observations. Clinical, laboratory, and hemodynamic data were collected at each time point, and SIC and DIC scores were calculated. The evolution of coagulopathy and its association with clinical and therapeutic variables were analyzed using appropriately adjusted generalized estimating equation (GEE) longitudinal models. Results: The prevalence of SIC increased from 36.8% at baseline to 47.5% at 24 h, and then declined to 20.8% at 96 h. DIC prevalence decreased from 21.7% to 9.7%. Coagulopathy at the previous time point was the main determinant of subsequent coagulopathy (SIC: OR 31.17; DIC: OR 67.17; p < 0.001). Incidence was highest during the early phases (SIC: 12.8% to 2.2%; DIC: 4.7% to 1.1%), whereas persistence decreased over time (SIC: 34.7% to 18.6%; DIC: 18.5% to 8.6%). Higher Sequential Organ Failure Assesment (SOFA) scores were associated with increased risk. Therapeutic anticoagulation was inversely associated with subsequent coagulopathy, including overt DIC positivity among patients without overt DIC at baseline (OR 0.059; 95% CI 0.012–0.290; p < 0.001). Higher cumulative fluid balance was associated with overt DIC in exploratory predicted-probability analyses, independent of vasopressor use, although this gradient did not reach statistical significance in adjusted models and is hypothesis-generating. Diuretic therapy was associated with an increased risk of SIC (OR 2.20; p = 0.008). Conclusions: Coagulopathy in sepsis occurs early and is strongly dependent on its initial trajectory. SIC and DIC represent stages of a continuum, with onset occurring predominantly within the first 24–48 h. The inverse association with therapeutic anticoagulation is hypothesis-generating and should not be interpreted as a treatment effect. Full article

Salinity restricts rice growth by disrupting cellular ion balance and promoting oxidative damage. Although exogenous melatonin can improve rice salt tolerance, whether expansion of the endogenous tryptophan pool enhances melatonin biosynthetic capacity and stress acclimation remains unclear. Here, we investigated a homozygous transgenic rice line constitutively expressing OASA1D, a feedback-insensitive D323N variant of the anthranilate synthase α-subunit OASA1. The OASA1D-expressing line exhibited strong resistance to 5-methyltryptophan and accumulated approximately twofold more tryptophan than wild-type plants in both shoots and roots under control and 150 mM NaCl conditions. The expanded tryptophan pool was accompanied by a 1.9–2.2-fold increase in endogenous melatonin and elevated expression of the melatonin biosynthetic genes OsTDC1, OsT5H, OsSNAT1, and OsASMT1. Under salt stress, OASA1D seedlings maintained greater shoot and root growth, biomass, and soil–plant analysis development (SPAD) values than wild-type seedlings. OASA1D also showed lower H₂O₂ and malondialdehyde accumulation and reduced electrolyte leakage, together with higher superoxide dismutase, catalase, and ascorbate peroxidase activities. Salt-induced expression of OsDREB2A, OsLEA3-1, OsP5CS1, OsWRKY45, OsHKT1;5, OsNHX1, and OsSOS1 was enhanced in OASA1D. Consistently, OASA1D shoots accumulated less Na⁺, retained more K⁺, and maintained a higher K⁺/Na⁺ ratio under salinity. Together, these results show that constitutive OASA1D expression expands the endogenous tryptophan pool and is associated with enhanced melatonin biosynthetic capacity, antioxidant defence, ionic homeostasis, and salt tolerance in rice. Full article

This study investigates cellulose nanofiber (CNF) suspension as a nano-reinforcement for underwater non-dispersible concrete, aiming to improve the balance among workability, washout resistance, and compressive-strength performance. An L9 orthogonal array was employed to evaluate the dosage-dependent responses of CNF suspension, hydroxypropyl methylcellulose (HPMC), and a polycarboxylate ether-based water-reducing admixture (PCE). Within the investigated range, increasing the as-received CNF-suspension dosage from 0.15% to 0.60% was associated with an increase in the factor-level mean 28-day underwater-to-air compressive-strength ratio from 0.75 to 0.79 and a decrease in suspension pH from 11.92 to 11.73, suggesting higher underwater strength retention and lower alkaline dispersion. CNF suspension showed favorable dosage-dependent responses in the washout-related and compressive-strength indicators, while HPMC and PCE contributed to the overall regulation of measured workability and compressive-strength performance. Among the nine mixtures, Group 5 was identified as a balanced engineering-oriented candidate, combining a slump of 245 mm, a slump flow of 465 mm, and 7- and 28-day underwater-to-air strength ratios of 0.94 and 0.82, respectively. SEM observations revealed pores, interfacial gaps, and locally distributed acicular, plate-like, and filament-like features, providing qualitative microstructural context for the measured responses. Overall, the results support the potential of CNF suspension as a promising nano-reinforcement for underwater non-dispersible concrete with a favorable balance of workability, washout-related performance, and compressive-strength retention. Full article

Phosphorus (P) deficiency is a major factor governing peanut (Arachis hypogaea L.) productivity, and the physiological mechanisms by which different genotypes (with contrasting photosynthetic capacities) coordinate carbon assimilation and photoprotection remain elusive. This study elucidated the strategic divergence among different peanut genotypes in their foliar photosystems to perform physiological homeostasis under low-phosphorus (LP) conditions. Based on a peanut mini-core collection, six representative accessions with contrasting photosynthetic capacities were selected and categorized into high- and low-photosynthetic functional groups. We integrated leaf gas exchange, chlorophyll fluorescence, the trans-thylakoid proton gradient (ΔpH), and antioxidant enzyme assays to evaluate their adaptive responses to low-P stress relative to the high-P (HP) control. Our results demonstrated that LP stress induced widespread photosynthetic inhibition across all accessions; this suppression was primarily driven by non-stomatal limitations. Under LP stress, high-Pn accessions exhibited superior cyclic electron flow (CEF) plasticity synergized with highly plastic guaiacol peroxidase (POD) activity, suppressing the leaf-level ROS burst and maintaining a substantial ΔpH for ATP synthesis and PSI stability. Conversely, low-Pn accessions suffered from severe oxidative overload and relied heavily on passive thermal dissipation, characterized by elevated non-photochemical quenching (NPQ) values and restricted CEF engagement. Principal component analysis (PCA) confirmed that while baseline biochemical impairments were universal, the capacity to dynamically modulate this ΔpH-dependent regulatory network—which integrates CEF, cytochrome b6f photosynthetic control, and antenna-level NPQ—served as the decisive determinant underlying genotypic variations in photosystem resilience under P deficiency. This study demonstrated that peanut genotypes deploy divergent, ΔpH-centered strategies to balance light energy distribution under P-limited conditions. These findings provide a novel and plausible mechanistic framework for selecting and breeding P-efficient peanut cultivars in poor soils with enhanced photosystem resilience. Full article

Retinol, a bioactive small molecule of the vitamin A family, contributes epidermal and dermal tissue repair through retinoic acid receptor γ (RARγ)/retinoid X receptor (RXR) receptor-mediated transcriptional regulation of keratinocyte differentiation, extracellular matrix (ECM) remodeling, and barrier restoration. However, the relationship between applied concentration, tissue-level regenerative outcomes, and tolerability remains incompletely characterized. This prospective, randomized, single-blind study compared biophysical effects and tolerance of two retinol concentrations in EU-compliant facial creams, high-performance liquid chromatography (HPLC)-verified as 0.104% and 0.247% (w/w). Thirty-eight women aged 40–61 years (Fitzpatrick phototypes II–III) participated across three independent sub-studies: 28 were randomized to either concentration for 12 weeks; 5 underwent split-face ultrasound imaging (0.247% versus retinol-free control) for 8 weeks; and 5 participated in a tape stripping sub-study quantifying stratum corneum interleukin-1 alpha (IL-1α) and interleukin-1 receptor antagonist (IL-1ra) by ELISA before and after 6 weeks of 0.247% retinol treatment. Main cohort assessments included transepidermal water loss (TEWL), hydration, melanin, erythema, pH, sebum, biomechanical parameters, and wrinkle grading. Both concentrations significantly improved barrier parameters—hydration, TEWL, brightness, pH, and sebum—with no inter-group differences. In the ultrasound sub-group, 0.247% retinol increased epidermal thickness (+12.4 μm), epidermal density (+3.84%), and dermal density (+1.81%) versus control, consistent with ECM reorganization and epidermal stratification. Biomechanical parameters showed no significant changes, consistent with retinol’s remodeling timeline. Consumer assessment indicated comparable efficacy; 0.247% demonstrated superior smoothing but higher erythema incidence. In the tape stripping sub-study, IL-1α decreased in all participants (median: 25.1 → 13.2 picograms per tape [pg/Tape]; 5/5 concordant), while IL-1ra increased in 4/5 participants (median: 352.8 → 1403.1 picograms per three sequential tapes [pg/3sT]), indicating a directionally consistent shift in the IL-1α/IL-1ra balance; these results are exploratory and require replication in larger cohorts. These findings collectively support clinically meaningful barrier restoration and structural remodeling within current EU safety limits (Commission Regulation EU 2024/996), with 0.104% offering a favorable efficacy-to-tolerability profile for initial therapy. Full article

Extensive livestock farming is characteristic of the landscape of the Mediterranean regions of Southern Iberian Peninsula. These production systems based on dryland pastures provide a wide range of services and contribute to maintaining environmental balance when compared with intensive agricultural or other livestock production systems. The Portuguese Montado is a habitat of Community importance and is protected under the European Natura 2000 network. This makes any research aimed at preserving, maintaining, or restoring this ecosystem particularly important to ensure its sustainable management. The main objectives of this study were to evaluate: (i) the impact of dolomitic limestone application on soil pH; (ii) the relation between multiple soil parameters; (iii) the impact of grazing preferences and livestock stocking rates on topsoil compaction; (iv) temporal and spatial sheep grazing patterns and sward productivity, quality and floristic composition throughout the growing vegetative season. This study was carried out during the vegetative cycle of 2023/2024 on a 4-ha pasture field located at Mitra farm (Southern Portugal). The experimental design included four treatments resulting from the combination of limestone application (with and without) and stocking rate (traditional: 7 sheep ha⁻¹; high: 18 sheep ha⁻¹). Sheep grazing preferences, soil compaction and fertility, sward productivity, quality and floristic composition were monitored at 48 sampling areas. The results confirm that improving soil pH through the application of dolomitic limestone is an effective, although slow and gradual process. When combined with grazing management through increased stocking rates, several important outcomes were observed: (i) preferential grazing areas did not exhibit significant differences in soil trampling; (ii) higher stocking rates resulted in less selective grazing; (iii) soil amendment and higher livestock stocking rates contributed to greater pasture crude protein content; and (iv) the influence of pasture quality on grazing preferences depended on the phase of the pasture-grazing cycle. Overall, these findings are promising indicators of sustainability for extensive animal production in Mediterranean dryland silvopastoral systems. However, with regard to the sward, as this study only monitored a single growing season, will need to be validated in future studies. Full article

Carbon nanotube field-effect transistors (CNT-FETs) are promising platforms for electrolyte-gated sensing, although the influence of CNT network density on device performance for pH measurements remains unknown. In this work, CNT-FETs with controlled network densities were fabricated from aqueous CNT solutions by varying the CNT concentration and the deposition time. Increasing CNT density improved conductivity, reducing channel resistance from the GΩ range to ~100 kΩ and increasing the on-current of electrolyte-gated devices from ~10 nA to ~1 µA. The fabricated CNT-FETs operating in a liquid-gated configuration exhibited on/off ratios ranging between 10³ and 10⁵. The minimum subthreshold swing achieved by the CNT-FETs was 77.5 mV/dec for devices employing a low-density CNT network, compared with 105 mV/dec for those incorporating a high-density CNT network. The high-density CNT networks also exhibited reduced electrostatic gate coupling due to charge screening effects. pH measurements in 1XPBS showed that low-density networks achieved the highest sensitivity of 8.9%/pH, whereas high-density networks showed lower sensitivity of 2.1%/pH, despite producing the largest absolute current response of ~70 nA. Medium-density networks provided the best balance between sensitivity, noise, and signal stability. These findings demonstrate that CNT network density is a critical parameter for optimizing electrolyte-gated CNT-FET pH sensors. Full article

Puccinellia tenuiflora is a perennial halophytic grass commonly regarded as a pioneer species for the ecological restoration of saline–alkali land. Its adaptive capacity and subsequent utilization value are shaped by interacting structural, physiological, molecular, ecological, and management-related factors. This review summarizes recent studies on saline–alkali tolerance in P. tenuiflora, with emphasis on root structural barriers, Na⁺/K⁺ homeostasis, osmotic adjustment, organic-acid metabolism, antioxidant defense, ion transport, and multi-omics regulation. To better understand the integrated stress response, we propose a functional framework that distinguishes first-line defenses from downstream cellular repair mechanisms. First-line defenses include root apoplastic barriers (Casparian strips and suberization) that restrict Na⁺ entry, plasma-membrane Na⁺/H⁺ antiporters (e.g., SOS1) that mediate active Na⁺ exclusion, and K⁺-retention mechanisms (e.g., AKT1, HKT2;1) that preserve cytosolic K⁺/Na⁺ homeostasis—these operate rapidly to prevent ion imbalance at the onset of stress. Downstream repair and acclimation mechanisms include osmotic adjustment via compatible solutes (e.g., proline, glycine betaine), organic-acid accumulation (especially citric acid) for pH regulation and chelation, ROS scavenging systems, and proteomic/phosphoproteomic reprogramming that repair stress-induced damage and restore metabolic balance. Furthermore, saline–alkali stress involves both short-term osmotic shock and long-term ionic toxicity, and available evidence suggests a temporal shift in the relative importance of these mechanisms: osmotic adjustment and rapid ion exclusion dominate during the initial hours to days of stress, whereas organic-acid metabolism, ROS buffering, and molecular reprogramming become increasingly important during prolonged exposure, sustaining tissue integrity and enabling long-term persistence. Current evidence indicates that saline–alkali tolerance in P. tenuiflora results from the combined action of several processes, including restricted Na⁺ entry, K⁺ retention, organic-acid accumulation, reactive oxygen species homeostasis, and organ-specific molecular responses. This review also discusses the significance of P. tenuiflora in community establishment, saline–alkali land restoration, and post-restoration forage utilization. Field studies and limited feeding trials suggest that P. tenuiflora can provide biomass and utilization potential after community stabilization. However, based on current evidence, it is more appropriate to define its forage value as a post-restoration utilization extension rather than as that of a fully developed specialized forage crop. Further studies are required on nutritional quality, mineral-element safety, long-term field management, and animal feeding validation. Full article

Heat stress (HS) impairs boar reproductive capacity via damaging Sertoli cells (SCs) and disrupting the integrity of the blood–testis barrier (BTB). This present study investigated the protective effects and underlying molecular mechanisms of PTD-FNK against HS-induced injury in porcine SCs. A HS model (43 °C, 1 h) was established, and 0.1 nM was determined as the optimal working concentration of PTD-FNK. Results showed that PTD-FNK effectively reversed HS-induced BTB disruption by restoring the expression levels of tight junction proteins (Claudin-1, Occludin, ZO-1, and Cx43) to baseline levels (p < 0.05). Concurrently, PTD-FNK alleviated HS-induced oxidative stress by enhancing total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) activity, while reducing malondialdehyde (MDA) content (p < 0.05). It also suppressed HS-triggered apoptosis by downregulating the expression of pro-apoptotic proteins (Caspase-3/8/9 and Bax) and upregulating the anti-apoptotic protein Bcl-2 (p < 0.05). Furthermore, PTD-FNK maintained cellular homeostasis by regulating mitochondrial dynamics—restoring mitochondrial membrane potential (MMP) and balancing the expression of fusion-related protein Mfn1 and fission-related protein Drp1.Transcriptomic and LY294002 experiments confirmed PTD-FNK exerted effects via the PI3K/AKT pathway, modulating ID3, H2AX, DDIT3, CDKN1C, RAD51, and TP53. Thus, PTD-FNK protects SCs from HS through multi-target regulation dependent on PI3K/AKT, providing a novel strategy for boar reproductive health under HS. Full article

This study evaluated molasses-based liquid feeds containing conventional urea or a commercial fat-coated urea product marketed as slow-release urea (SRU). The effects on in vitro gas-production kinetics, rumen fermentation, and substrate degradability were examined. Treatments followed a 5 × 3 factorial design. The five formulations were 0% urea, 5% urea, 10% urea, 5% SRU, and 10% SRU. Each formulation was supplied at 0.9%, 1.8%, or 2.7% of the 0.5 g basal substrate dry matter (DM). Four independent incubation runs were conducted. A formulation × inclusion-level interaction was detected for gas-production lag time (p = 0.0046). The shortest lag time occurred with 10% SRU supplied at 2.7%. At 48 h, the 0.9% inclusion level resulted in greater dry matter and organic matter degradability than the 1.8% and 2.7% levels. The 5% SRU formulation had the lowest 24 h degradability, whereas 10% SRU had the greatest 48 h dry matter degradability. Ammonia nitrogen (NH₃-N) was analyzed separately at each sampling time. The 10% conventional urea formulation had the greatest NH₃-N concentration at 1 and 2 h. The 10% SRU formulation had the greatest concentration at 4, 6, and 12 h. These within-time differences do not demonstrate different ammonia-release patterns over time. At 12 h, the 10% SRU formulation had the greatest total volatile fatty acid concentration and propionate molar proportion. Protozoal counts were unaffected. The results reflect differences among the complete liquid-feed formulations. They do not provide direct evidence of controlled ammonia release or improved nitrogen–carbohydrate synchronization. Further in vivo studies using compositionally balanced formulations are required. Full article

Balancing food production, ecosystem service value, and agricultural irrigation water consumption is a major challenge for sustainable agricultural development in China. However, quantitative evidence at the national scale remains limited on whether crop planting structure optimization derived from models can effectively achieve coordination among these three objectives. Existing studies mainly focus on individual crops or localized regions and rarely integrate the spatiotemporal evolution, influencing factors, and multi-objective optimization of major staple crops within a unified framework. This study developed a progressive framework integrating spatiotemporal evolution analysis, influencing factor identification, and planting structure optimization for wheat, rice, and maize. Spatial autocorrelation analysis, center-of-gravity shift analysis, and pixel-based image differencing were applied to reveal crop evolution patterns across China from 2000 to 2025. A five-dimensional indicator system comprising 17 quantitative indicators was developed through multiple experiments using four large language models, and the Random Forest algorithm was employed to identify key influencing factors and their relative importance. Based on these factors, optimization constraints were constructed, and a multi-objective fuzzy linear programming model combined with the NSGA-II algorithm was used to determine optimal crop area allocation across 28 provincial-level regions. The three staple crops exhibited a significant pattern of northward shift, eastward expansion, and southern contraction. Precipitation and market accessibility were common core influencing factors, ranking among the top five factors in all nine Random Forest models. Crop-specific factors, including soil available phosphorus for rice, accumulated active temperature for wheat, and soil pH for maize, explained differences in spatial responses among crops and provided a scientific basis for optimization modeling and coordinated improvement of food production, ecosystem service value, and irrigation water consumption. The optimized scheme increased total grain output by 3.6%, improved ecosystem service value by 11.0%, and reduced irrigation water consumption by 45.7% compared with the actual planting structure, all 28 provinces achieved improvement or stability in the three indicators simultaneously. Based on optimized crop allocation patterns, national planting structures were summarized into regional models, including a rice–maize dual-core system in Northeast China, wheat–maize rotation in the Huang-Huai-Hai Plain, rice-dominated systems in the middle and lower Yangtze River Basin and South China, water-efficient dryland farming in Northwest China, and a diversified balanced system in Southwest China. These findings provide a quantitative reference for optimizing China’s staple crop planting structure. Full article

Extremophilic prokaryotes survive salt, temperature, and pH extremes by coordinating stress-induced protein networks that preserve macromolecules, sustain energetics, and repair damage. This review integrates recent proteomics with functional genomics to resolve both network state and causality across halophiles, thermophiles, acidophiles, alkaliphiles, psychrophiles, and radiation-resistant prokaryotes. Quantitative proteomics maps condition-specific induction of chaperones, proteases, ion transporters, osmolyte pathways, DNA repair proteins, antioxidants, and envelope remodeling enzymes. Complementary perturbation genetics, functional genomics, and transcriptomics help to identify essential nodes and regulatory circuits underlying stress tolerance. In halophiles, compatible solute synthesis and Na⁺/H⁺ exchange couple to protein quality control and central metabolism, whereas many archaeal halophiles additionally rely on high intracellular salt and distinctive membrane chemistry. Thermophiles rely on heat-shock systems, ATP-dependent proteolysis, membrane adjustments, and redox balancing. Acidophiles maintain near-neutral cytosol via proton export and low-permeability membranes while linking iron handling to oxidative defense. Alkaliphiles use Na⁺-based bioenergetics, multi-subunit antiporters, and cell-wall modifications to retain protons. Psychrophiles emphasize cold-shock RNA chaperones, flexible enzymes, and cryoprotectants, whereas radiophiles combine exceptional DNA repair with strong antioxidant capacity. Across taxa, oxidative stress forms a cross-cutting axis that explains extensive regulon overlap and cross-protection. We synthesize network architecture, highlight conserved modules and lineage-specific solutions, and outline open questions in stress sensing, multi-stress integration, and the functions of uncharacterized proteins. These insights provide a framework for engineering robust biocatalysts and organisms for biotechnology and environmental applications. Full article