Search Results (50)

Osteoporosis is a multifactorial, polygenic disease characterized by reduced bone mineral density (BMD) and increased fracture risk. Genome-wide association studies (GWASs) have identified numerous loci associated with BMD and/or bone fractures, but functional characterization of these target genes is essential to understand the biological mechanisms underlying osteoporosis. This review focuses on current methodologies and key examples of successful functional studies aimed at evaluating gene function in osteoporosis research. Functional evaluation typically follows a multi-step approach. In silico analyses using omics datasets expression quantitative trait loci (eQTLs), protein quantitative trait loci (pQTLs), and DNA methylation quantitative trait loci (mQTLs) help prioritize candidate genes and predict relevant biological pathways. In vitro models, including immortalized bone-derived cell lines and primary mesenchymal stem cells (MSCs), are used to explore gene function in osteogenesis. Advanced three-dimensional culture systems provide additional physiological relevance for studying bone-related cellular processes. In situ analyses of patient-derived bone and muscle tissues offer validation in a disease-relevant context, while in vivo studies using mouse and zebrafish models enable comprehensive assessment of gene function in skeletal development and maintenance. Integration of these complementary methodologies helps translate GWAS findings into biological insights and supports the identification of novel therapeutic targets for osteoporosis. Full article

The efficiency of coalbed methane (CBM) extraction is closely related to the drilling response of coal seams, which is significantly influenced by natural fracture development of coal seams. This work investigated 11 coal samples from the Baode, Xinyuan, and Huolinhe mines, employing quantitative fracture characterization, acoustic wave testing, drilling experiments, and cuttings analysis to systematically reveal the relationships and mechanisms between fracture parameters and coal drilling response characteristics. The result found that acoustic parameters (average wave velocity v and drilling surface wave velocity v₀) exhibit significant negative correlations with fracture line density (ρ₁) and area ratio (ρ₂) (|r| > 0.7), while the geological strength index (GSI) positively correlates with acoustic parameters, confirming their utility as indirect indicators of fracture development. Fracture area ratio (ρ₂) strongly correlates with drilling cuttings rate q (r = 0.82), whereas GSI negatively correlates with drilling rate w, indicating that highly fractured coal is more friable but structural stability constrains drilling efficiency, while fracture parameters show limited influence on drill cuttings quantity Q. Cuttings characteristics vary with fracture types and density. Type I coal (low-density coexisting exogenous fractures and cleats) produces cuttings dominated by fine particles with concentrated size distribution (average particle size d ≈ 0.52 mm, crushability index n = 0.46–0.61). Type II coal (exogenous-fracture-dominant) exhibits coarser particle sizes in cuttings (d ≈ 0.8 mm, n = 0.43–0.53). Type III coal (dense-cleat-dominant) drill cuttings are mainly coarse particles and are concentrated in distribution (d ≈ 1.53 mm, n = 0.72–0.98). Additionally, drilling response differences are governed by the coupling effects of vitrinite reflectance (R_o), density, and firmness coefficient (f), with Huolinhe coal being easier to drill due to its lower R_o, f, and density. This study elucidates the mechanism by which fracture development affects coal drilling response through multi-parameter correlation analysis, while also providing novel insights into the optimization of fracturing sweet spot selection for CBM development. Full article

Fine-grained Yb:Y₂O₃ laser ceramics with excellent transmittance and thermal conductivity were fabricated from commercial powders. The process involved aqueous colloidal forming, additive-free air pre-sintering at 1400 °C, and hot isostatic pressing at 1550 °C. Suspensions were prepared with a deionization process to alleviate the hydrolysis issue, which optimizes the microstructure uniformity and enhances the green compacts’ density after consolidation. The microstructure, in-line transmittance, microhardness, and fracture toughness of the Yb³⁺-doped Y₂O₃ ceramics with different concentrations were measured. The 5.0 at% Yb³⁺-doped Y₂O₃ ceramic yielded a superior transmittance of 80.1% at 1100 nm and 83.0% in the mid-infrared region. The average grain size was 752 nm. The sample exhibited a thermal conductivity of 9.94 W·m⁻¹·K⁻¹ while achieving a 1076 nm laser output with a 42 mW peak power and 4.3% slope efficiency. Full article

Fluid flow and mass transfer processes in some fractured aquifers are negligible in the low-permeability rock matrix and occur mainly in the fracture network. In this work, we consider coupled variable-density flow (VDF) and mass transport with dissolution in discrete fracture networks (DFNs). These three processes are ruled by nonlinear and strongly coupled partial differential equations (PDEs) due to the (i) density variation induced by concentration and (ii) fracture aperture evolution induced by dissolution. In this study, we develop an efficient model to solve the resulting system of nonlinear PDEs. The new model leverages the method of lines (MOL) to combine the robust finite volume (FV) method for spatial discretization with a high-order method for temporal discretization. A suitable upwind scheme is used on the fracture network to eliminate spurious oscillations in the advection-dominated case. The time step size and the order of the time integration are adapted during simulations to reduce the computational burden while preserving accuracy. The developed VDF-DFN model is validated by simulating saltwater intrusion and dissolution in a coastal fractured aquifer. The results of the VDF-DFN model, in the case of a dense fracture network, show excellent agreement with the Henry semi-analytical solution for saltwater intrusion and dissolution in a coastal aquifer. The VDF-DFN model is then employed to investigate coupled flow, mass transfer and dissolution for an injection/extraction well pair problem. This test problem enables an exploration of how dissolution influences the evolution of the fracture aperture, considering both constant and variable dissolution rates. Full article

This study departs from conventional stochastic statistical approaches for rock mass structural modeling. Based on deterministic structural surface parameters, including orientation (dip and dip direction), trace length, trace center coordinates, and spacing between structural surface sets, this research investigates the relationships among volumetric density, areal density, structural surface persistence, and inter-set spacing. With a focus on model domain dimensions, positioning of the model center, and mitigation of boundary effects, the methodology systematically addresses key considerations in modeling joints, layers, and faults. A deterministic Discrete Fracture Network (DFN) modeling approach is proposed accordingly. In this framework, joints are represented by disks, whereas lithological interfaces such as layers and faults are modeled as flat planes. The proposed method was applied to the Qingdao Metro Line 15 project. Validation results demonstrate that the surrounding rock classification derived from the model is in good agreement with field geological investigation data. Full article

Composite pressure vessels offer significant advantages over traditional metal-lined designs due to their high strength-to-weight ratio, corrosion resistance, and design flexibility. This study investigates the structural design, winding process, finite element analysis, and experimental validation of a glass fiber-reinforced composite low-pressure vessel. A high-density polyethylene (HDPE) liner was designed with a nominal thickness of 1.5 mm and manufactured via blow molding. The optimal blow-up ratio was determined as 2:1, yielding a wall thickness distribution between 1.39 mm and 2.00 mm under a forming pressure of 6 bar. The filament winding process was simulated using CADWIND software (version 10.2), resulting in a three-layer winding scheme consisting of two helical layers (19.38° winding angle) and one hoop layer (89.14°). The calculated thickness of the composite winding layer was 0.375 mm, and the coverage rate reached 107%. Finite element analysis, conducted using Abaqus, revealed that stress concentrations occurred at the knuckle region connecting the dome and the cylindrical body. The vessel was predicted to fail at an internal pressure of 5.00 MPa, primarily due to fiber breakage initiated at the polar transition. Experimental hydrostatic burst tests validated the simulation, with the vessel exhibiting failure at an average pressure of 5.06 MPa, resulting in an error margin of only 1.2%. Comparative tests on vessels without adhesive sealing at the head showed early failure at 2.46 MPa, highlighting the importance of head sealing on vessel integrity. Scanning electron microscopy (SEM) analysis confirmed strong fiber–matrix adhesion and ductile fracture characteristics. The close agreement between the simulation and experimental results demonstrates the reliability of the proposed design methodology and validates the use of CADWIND and FEA in predicting the structural performance of composite pressure vessels. Full article

In the context of global urbanization and the concomitant tension between heritage conservation and urban development, there is an urgent need to explore effective strategies for addressing the challenges posed by fragmented conservation, static cognition, and homogeneous renewal in conservation practice. Utilizing the theoretical framework of urban historic landscape, this study integrates urban morphology, architectural typology, urban imagery, and catalyst theory to formulate a progressive study on the evolution of historic districts through the layers of “historic areas, spatial forms, material carriers, value characteristics”. The research path is a progressive one that analyses the regularity of historic districts. The present study focuses on Shenyang as the object of empirical research, employing a multifaceted research method that integrates multiple scenarios and sub-cases within a single case. This method utilizes a combination of the literature and field research to obtain diversified data. The study then undertakes a systematic analysis of the accumulation of Shenyang’s historic districts through the application of kernel density analysis and geometric graphical methods. The study found that the dimension of the historical area of the Shenyang historic district presents the layering law of “single-core dominant–dual-core juxtaposition–fusion collage–extension–multi-point radiation”, and that the spatial form is summarized as seven types of the layering law, such as radiation type, ring type, triangular type, and grid type. The spatial form is summarized into seven types of laminar laws, such as radial, ring, triangular, grid, etc. The material carriers exhibit the conventional law of anchoring point-like elements, employing line-like elements as the skeletal structure and surface-like elements as the matrix. The value laminations are diversified, centralized, and self-adaptive. The study proposes the concept of “layer accumulation law” to elucidate the carrier transformation mechanism of cultural genes, and it provides a methodological tool for addressing the dilemma of “layer accumulation fracture”. The findings of this study not only deepen the localized application of HUL theory but also provide an innovative path for the practice of heritage conservation in urban renewal. Full article

Though circular exercise is commonly used in equestrian disciplines, it may be at the detriment of horses’ musculoskeletal system. To investigate the effects of circular exercise on bone and joint health, 42 lambs were randomly assigned to a non-exercised control, straight-line, small circle, or large circle exercise regime at a slow (1.3 m/s) or fast (2.0 m/s) speed for 12 wk. Blood samples were taken biweekly. Animals were humanely euthanized upon study completion, and the fused third and fourth metacarpals were collected for biomechanical testing and bone density analysis. Fast groups were found to have more bone formation and less resorption activity than slow groups as evidenced by serum biomarker concentrations (p < 0.05). Sheep in the large fast group tended to have greater flexural rigidity and fracture force for the outside leg compared to the inside leg (p < 0.1). Sheep in the small slow group tended to have increased bone mineral density of the outside leg compared to the inside leg, whereas the opposite occurred in the large slow group (p < 0.1). These results provide further evidence for potential asymmetric musculoskeletal adaptations to circular exercise while emphasizing the importance of speed as a positive influence on bone metabolism and strength. Full article

The medium development characteristics and controlling factors of the karst peak forest plain water system constitute the core of analyzing the complex and variable hydrogeological environment, especially in revealing the controlling factors between the hydrological system and karst development characteristics, which is crucial for a deeper understanding of karst hydrogeological environments. This study takes Zengpiyan in Guilin as an example and conducts a dynamic clustering analysis on the advantageous occurrence of fracture development in three sampling areas. A total of 3472 karst channels and fractures were identified and measured. Our research reveals the following: (1) The high degree of development of fissures on surface rock outcrops is mainly formed by the expansion of shear joints through dissolution and erosion. The dip angles of fissures are mainly characterized by low angles, with fissures with dip angles between 18° and 80° accounting for 65.44% of the total observed fissures. The linear density of fissures is 3.64 per meter. (2) There are significant differences in the line density of cracks and fissures in different areas of the research area. For example, the line density in Sampling Area 1 is 0.99 lines per meter, while the line density in Sampling Area 3 reaches 5.02 lines per meter. In addition, the extension length of cracks is generally long, with joints with extension lengths exceeding 1.5 m accounting for 77.46% of the total observed joints and through cracks with extension lengths exceeding 5 m accounting for 23.33%. (3) The development characteristics of underground karst reveal that underground karst caves are mainly distributed at elevations of 120 to 160 m, with a drilling encounter rate of about 43.3%. It is also noted that geological structures control the horizontal distribution of karst, and geological lithology, hydrodynamic conditions, and water carbon dioxide concentrations are key factors affecting the vertical zoning of karst. This study provides an important scientific basis for understanding the development characteristics and controlling factors of karst water system media in peak forest plains and has important guiding significance for water resource management in karst areas and disaster prevention during tunnel excavation. Full article

Osteoporosis is a progressive metabolic bone disease characterized by decreased bone density and microarchitectural deterioration, leading to an increased risk of fracture, particularly in postmenopausal women and the elderly. Increasing evidence suggests that inflammatory processes play a key role in the pathogenesis of osteoporosis and are strongly associated with the activation of osteoclasts, the cells responsible for bone resorption. In the present study, we investigated, for the first time, the influence of proinflammatory cytokines on the osteogenic differentiation, proliferation, and metabolic activity of primary human osteoblast-like cells (OBs) derived from the femoral heads of elderly patients. We found that all the proinflammatory cytokines, IL-1β, TNF-α, IL-6, and IL-8, enhanced the extracellular matrix mineralization of OBs under differentiation-induced cell culture conditions. In the cases of IL-1β and TNF-α, increased mineralization was correlated with increased osteoblast proliferation. Additionally, IL-1β- and TNF-α-increased osteogenesis was accompanied by a rise in energy metabolism due to improved glycolysis or mitochondrial respiration. In conclusion, we show here, for the first time, that, in contrast to findings obtained with cell lines, mesenchymal stem cells, or animal models, human OBs obtained from patients exhibited significantly enhanced osteogenesis upon exposure to proinflammatory cytokines, probably in part via a mechanism involving enhanced cellular energy metabolism. This study significantly contributes to the field of osteoimmunology by examining a clinically relevant cell model that can help to develop treatments for inflammation-related metabolic bone diseases. Full article

In order to fully explore the development degree and distribution law of the structural plane of a tunnel surrounding rock in three-dimensional space, this paper studies the geometric characteristic parameters of a structural plane in the study area through field investigation, data acquisition and statistical analysis. The structural plane is divided into three dominant groups by using DIPS. v5. 103 software. The probability distribution model of occurrence, trace length, diameter and spacing of the structural plane is established. This paper focuses on the error correction of structural plane occurrence and the estimation of average trace length based on the rectangular window method. The discrete fracture network model is generated by using MATLAB R2021b software, and the discrete fracture network model is verified from three aspects: structural plane occurrence, average trace length and area density. The verification results are compared with the measured data, and the simulation results are in line with the actual situation on site. Based on the discrete fracture network model, the volume joint number of rock mass is calculated. Based on the JSR index, BQ classification method and RQD classification, the development degree of fractures and surrounding rock classification in this area are evaluated. A method of surrounding rock classification based on three evaluation indexes is discussed to comprehensively and accurately classify the quality of rock mass in this area. Full article

Roughness is an essential factor affecting the shear process of discontinuous surfaces, and the evolution of roughness is closely related to the mechanical behavior of discontinuous surfaces. In this paper, with the help of granite specimens, a direct shear test was carried out on flat fracture surfaces obtained by sawing in order to study the evolution of roughness with shear slip. During the tests, the roughness evolution was evaluated using the arithmetic mean, root mean square and power spectral density of the roughness. The variation in these parameters all indicate that the friction surface with large slip tends to be rougher, at least under the loading conditions in this paper. And the increase in normal force will enhance this process, while the loading rate seems to have little effect on the roughness evolution. Finally, the analysis of the power spectral density shows that the roughness evolution in the spatial frequency of the profile line is mainly reflected in the middle– and low–frequency part, while the high–frequency part corresponding to the microscopic roughness body does not change much throughout the shear process. Full article

The primary focus of the current paper centers on the microstructures and mechanical properties exhibited by a Ti-30Nb-12Zr-5Ta-2Sn-1.25Fe (wt. %) (TNZTSF) alloy that has been produced through an intricate synthesis process comprising cold-crucible induction in levitation, carried out in an atmosphere controlled by argon, and cold-rolling deformation (CR), applying systematic adjustments in the total deformation degree (total applied thickness reduction), spanning from 10% to 60%. The microstructural characteristics of the processed specimens were investigated by SEM and XRD techniques, and the mechanical properties by tensile and microhardness testing. The collected data indicate that the TNZTSF alloy’s microstructure, in the as-received condition, consists of a β-Ti phase, which shows polyhedral equiaxed grains with an average grain size close to 82.5 µm. During the cold-deformation processing, the microstructure accommodates the increased applied deformation degree by increasing crystal defects such as sub-grain boundaries, dislocation cells, dislocation lines, and other crystal defects, powerfully affecting the morphological characteristics. The as-received TNZTSF alloy showed both high strength (i.e., ultimate tensile strength close to σ_UTS = 705.6 MPa) and high ductility (i.e., elongation to fracture close to ε_f = 11.1%) properties, and the computed β-Ti phase had the lattice parameter a = 3.304(7) Å and the average lattice microstrain ε = 0.101(3)%, which are drastically influenced by the applied cold deformation, increasing the strength properties and decreasing the ductility properties due to the increased crystal defects density. Applying a deformation degree close to 60% leads to an ultimate tensile strength close to σ_UTS = 1192.1 MPa, an elongation to fracture close to ε_f = 7.9%, and an elastic modulus close to 54.9 GPa, while the computed β-Ti phase lattice parameter becomes a = 3.302(1) Å. Full article

The Laser Beam Welding (LBW) of aluminum alloys has attracted significant interest from industrial sectors, including the shipbuilding, automotive and aeronautics industries, as it expects to contribute to significant cost reduction associated with the production of high-quality welds. To comprehend the behavior of welded structures in regard to their damage tolerance, the application of fracture mechanics serves as the instrumental tool. However, the methods employed overlook the changes in the microstructure within the Heat-Affected Zone (HAZ), which leads to the degradation of the mechanical properties of the material. The purpose of this study is to simulate microhardness evolution in the HAZ of AA2198-T351 LBW. The material represents the latest generation of Al-Cu-Li alloys, which exhibit improved mechanical properties, enhanced damage tolerance behavior, lower density and better corrosion and fatigue crack growth resistance than conventional Al-Cu alloys. In this work, the microhardness profile of LBW AA2198 was measured, and subsequently, through isothermal heat treatments on samples, the microhardness values of the HAZ were replicated. The conditions of the heat treatments (T, t) were selected in line with the thermal cycles that each area of the HAZ experienced during welding. ThermoCalc and DICTRA were employed in order to identify the strengthening precipitates and their evolution (dissolution and coarsening) during the weld thermal cycle. The microstructure of the heat-treated samples was studied employing LOM and TEM, and the strengthening precipitates and their characteristics (volume fraction and size) were defined and correlated to the calculations and the experimental conditions employed during welding. The main conclusion of this study is that it is feasible to imitate the microstructure evolution within the HAZ through the implementation of isothermal heat treatments. This implies that it is possible to fabricate samples for fatigue crack growth tests, enabling the experimental examination of the damage tolerance behavior in welded structures. Full article

Sacral insufficiency fractures commonly affect elderly women with osteoporosis and can cause debilitating lower back pain. First line management is often with conservative measures such as early mobilization, multimodal pain management, and osteoporosis management. If non-operative management fails, sacroplasty is a minimally invasive intervention that may be pursued. Candidates for sacroplasty are patients with persistent pain, inability to tolerate immobilization, or patients with low bone mineral density. Before undergoing sacroplasty, patients’ bone health should be optimized with pharmacotherapy. Anabolic agents prior to or in conjunction with sacroplasty have been shown to improve patient outcomes. Sacroplasty can be safely performed through a number of techniques: short-axis, long-axis, coaxial, transiliac, interpedicular, and balloon-assisted. The procedure has been demonstrated to rapidly and durably reduce pain and improve mobility, with little risk of complications. This article aims to provide a narrative literature review of sacroplasty including, patient selection and optimization, the various technical approaches, and short and long-term outcomes. Full article