Search Results (5)

In today’s medicine, the celerity of the bio-assays analysis is crucial for the timely selection of the appropriate therapy and hence its effectiveness, especially in case of diseases characterized by the late onset of symptoms. In this paper, a lens-less fiber optics-based fluorescence sensor designed for the measurement of labeled bio-assays is presented and its potential for the early diagnosis of sepsis via C-reactive protein (CRP) detection is demonstrated. The sensor performance results from the combination of two key elements: a planar antenna that redirects fluorescence the marker emission and an automated fiber-based optical system for multi-spot analysis. First, the working principle of the device is demonstrated with a well-established antibody–antigen format (immunoglobulin IgG/anti-IgG assay), reporting more than one order of magnitude enhanced limit of detection (LOD) and limit of quantification (LOQ) for the planar antenna with respect to a standard glass substrate. The prototype is then tested against a sample mimicking a realistic case, prepared with commercially available human serum, showing a LOD and LOQ in the clinical range of interest (0.0015 μg/mL and 0.005 μg/mL, respectively) for the investigation of the sepsis biomarker CRP. These results validate the developed prototype as a simple and easy-to-operate device, compatible with standardized micro-well arrays, and potentially suitable for POC applications. Full article

Infrared imaging spectrometers detect and identify targets by collecting spectral and image information. However, when detecting small temperature differences and dynamic targets, the accuracy of infrared detection is reduced, the traditional scanning structure detection time is longer, the real-time performance is poor and it is easy to introduce motion artifacts. This paper proposes an infrared polarization snapshot spectral imaging system (PSIFTIS) based on a polarizer array, a lens array and a roof-shaped stepped micromirror. Polarized light can solve the problem of small-temperature-difference target recognition by characterizing the surface properties of materials. Lens arrays utilize multi-aperture imaging to achieve snapshot detection of targets. The system can obtain 4D data information, including polarization, in a single measurement cycle. This study completed the overall optical design of a PSIFTIS and an optical simulation experiment using it. Finally, a system prototype was built in the laboratory and a polarization spectrum detection experiment was carried out. The experimental results show that the PSIFTIS could accurately obtain the polarization spectrum information for the target, the spectral resolution reached 7.8 cm⁻¹ and the Stokes measurement error was less than 5%. Full article

Synchronous measurement of full-field temperature and deformation at high temperature especially more than 1273 K is of much significance especially for part applications of turbine structures and materials. Non-contact optical methods attract more and more attention, however, current methods all face different challenges, such as strong light reflection on the surface of the specimen, disturbing radiation from environment, complex equipment setup, limited measured temperature not higher than 900 °C and so on. In this work, we develop an innovative technique to overcome some current problems. The measurement system employing an ultraviolet (UV) imaging system is composed of a scientific complementary metal oxide semiconductor (sCMOS) camera, a lens and a UV bandpass filter. The UV bandpass filter was used for thermal radiation elimination to acquire high quality images at elevated temperatures for deformation field calculation suitable for digital image correlation (DIC) method. The UV sensitive sCMOS camera without using active illumination was employed to collect enough UV radiation energy and eliminate the interference of the external ambient light, which is applicable for high accuracy temperature field measurement. Our system can realize the synchronous capture of image and temperature acquisition with passive UV imaging system at temperature not lower than 1473 K. The feasibility of the method was verified through heating molybdenum (Mo) and Ni-based superalloy IC21 materials. The temperature fields of Mo measured by the established imaging system up to 1835 K with error less than 0.25% showed the effectiveness for temperature measurement. The estimated deformation and temperature field of Ni-based superalloy IC21 up to 1473 K with measured temperature error less than 0.5% demonstrated well the great potential of the UV imaging system in simultaneous measurement of temperature and deformation fields at elevated temperatures. Full article

A simple and versatile continuous air-segmented flow sensor using immobilized luciferase was designed as a general miniaturized platform based on sensitive biochemiluminescence detection. The device uses miniaturized microperistaltic pumps to deliver flows and compact sensitive light imaging detectors based on BI-CMOS (smartphone camera) or CCD technology. The low-cost components and power supply make it suitable as out-lab device at point of need to monitor kinetic-related processes or ex vivo dynamic events. A nylon6 flat spiral carrying immobilized luciferase was placed in front of the detector in lensless mode using a fiber optic tapered faceplate. ATP was measured in samples collected by microdialysis from rat brain with detecting levels as low as 0.4 fmoles. The same immobilized luciferase was also used for the evaluation of bile salt hydrolase (BSH) activity in intestinal microbiota. An aminoluciferin was conjugatated with chenodeoxycholic acid forming the amide derivative aLuc-CDCA. The hydrolysis of the aLuc-CDCA probe by BSH releases free uncaged aminoluciferin which is the active substrate for luciferase leading to light emission. This method can detect as low as 0.5 mM of aLuc-CDCA, so it can be used on real faecal human samples to study BSH activity and its modulation by diseases and drugs. Full article

In this paper, we propose a side-absorption concentrated module with diffractive grating as a spectral-beam-splitter to divide sunlight into visible and infrared parts. The separate solar energy can be applied to different energy conversion devices or diverse applications, such as hybrid PV/T solar systems and other hybrid-collecting solar systems. Via the optimization of the geometric parameters of the diffractive grating, such as the grating period and height, the visible and the infrared bands can dominate the first and the zeroth diffraction orders, respectively. The designed grating integrated with the lens and the light-guide forms the proposed module, which is able to export visible and infrared light individually. This module is demonstrated in the form of an array consisting of seven units, successfully out-coupling the spectral-split beams by separate planar ports. Considering the whole solar spectrum, the simulated and measured module efficiencies of this module were 45.2% and 34.8%, respectively. Analyses of the efficiency loss indicated that the improvement of the module efficiency lies in the high fill-factor lens array, the high-reflectance coating, and less scattering. Full article