DNA damage response (DDR) pathways in keto-enol genotoxicity have not been characterized, and few studies have reported genotoxic effects in non-target organisms. The present study shows that concentrations of 11.2, 22.4, 37.3 mg/L of Movento
® 240SC and 12.3, 24.6, 41.1 mg/L of
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DNA damage response (DDR) pathways in keto-enol genotoxicity have not been characterized, and few studies have reported genotoxic effects in non-target organisms. The present study shows that concentrations of 11.2, 22.4, 37.3 mg/L of Movento
® 240SC and 12.3, 24.6, 41.1 mg/L of Envidor
® 240SC for 72 h oral exposure induced DSBs by significantly increasing the percentage of γH2AV expression in regions 2b and 3 from the germarium of wild type females of
Drosophila melanogaster Oregon R, compared to the control group (0.0 mg/L of insecticides), via confocal immunofluorescence microscopy. The comparison between both insecticides’ reveals that only the Envidor
® 240SC induces concentration-dependent DNA damage, as well as structural changes in the germarium. We determined that the DDR induced by Movento
® 240SC depends on the activation of the
ATMtefu,
Chk1grp and
Chk2lok kinases by significantly increasing the percentage of expression of γH2AV in regions 2b and 3 of the germarium, and that
ATRmei−29D and
p53dp53 kinases only respond at the highest concentration of 37.3 mg/L of Movento
® 240SC. With the Envidor
® 240SC insecticide, we determined that the DDR depends on the activation of the
ATRmei−29D/Chk1grp and
ATMtefu/Chk2lok kinases, and
p53dp53 by significantly increasing the percentage of expression of γH2AV in the germarium.
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