Search Results (11)

Eutrophication and its resultant cyanobacterial blooms are a severe environmental issue in global water bodies, and phosphate is regarded as one of the primary triggers. In this study, the in situ-synthesized heated kaolinite lanthanum hydroxide composite (HKL-LH) was used to treat cyanobacterial blooms through phosphate removal. A typical cyanobacteria species—Microcystis aeruginosa—was selected as the target organism. HKL-LH efficiently removed phosphate in the solution with the inoculation of M. aeruginosa over the course of one day. A good performance of HKL-LH on control cyanobacterial blooms with initial cell densities ranging from 10⁴ cells mL⁻¹ to 10⁵ cells mL⁻¹ was observed. Although the genetic expression relating to photosynthesis and cell division was upregulated under the stress of phosphorus deficiency, M. aeruginosa growth was significantly inhibited, i.e., the inhibition rate of up to 98% was achieved by 0.1g L⁻¹ of HKL-LH. In addition to cell growth, the photosynthetic activity and viability of M. aeruginosa cells were decreased by HKL-LH. Furthermore, the production of associated toxins (microcystins) and algal organic matters were effectively inhibited, which can reduce the ecological risk and challenges that follow water treatment. In this study, it is shown that HKL-LH has excellent application potential in the mitigation of cyanobacterial blooms in eutrophic water. Full article

Diarrhetic shellfish poisoning (DSP) toxins and pectenotoxins (PTXs) produced by endemic species of Dinophysis, mainly D. acuta and D. acuminata, threaten public health and negatively impact the shellfish industry worldwide. Despite their socioeconomic impact, research on the environmental drivers of DSP outbreaks in the Chilean fjords is scanty. From 22 to 24 March 2017, high spatial–temporal resolution measurements taken in Puyuhuapi Fjord (Northern Patagonia) illustrated the short-term (hours, days) response of the main phytoplankton functional groups (diatoms and dinoflagellates including toxic Dinophysis species) to changes in water column structure. Results presented here highlight the almost instantaneous coupling between time–depth variation in density gradients, vertical shifts of the subsurface chlorophyll maximum, and its evolution to a buoyancy-driven thin layer (TL) of diatoms just below the pycnocline the first day. A second shallower TL of dinoflagellates, including Dinophysis acuta, was formed on the second day in a low-turbulence lens in the upper part of the pycnocline, co-occurring with the TL of diatoms. Estimates of in situ division rates of Dinophysis showed a moderate growth maximum, which did not coincide with the cell density max. This suggests that increased cell numbers resulted from cell entrainment of off-fjord populations combined with in situ growth. Toxin profiles of the net tow analyses mirrored the dominance of D. acuminata/D. acuta at the beginning/end of the sampling period. This paper provides information about biophysical interactions of phytoplankton, with a focus on Dinophysis species in a strongly stratified Patagonian fjord. Understanding these interactions is crucial to improv predictive models and early warning systems for toxic HABs in stratified systems. Full article

In this study, we analyzed in situ data from the years 2018–2022 encompassing entire forest plantations in Poland. Based on data regarding stand density and the occurrence of fungal, water-related, climate-related, fire, and insect factors that may intensify with climate changes, we determined the correlation between their occurrence and the decline in wood increments for six tree species: pine, birch, oak, spruce, beech, and alder. Subsequently, we identified age intervals in which the species–factor interaction exhibited statistically significant effects. Next, we developed neural network models for short-term wood increment predictions. Utilizing these models, we estimated a reduction in wood supply harvested in accordance with the plans for the years 2023–2025 assuming a tenfold greater intensity of factors than in 2022. Findings indicate: birch: water-related factors may reduce wood production by 0.1%–0.2%. This aligns with previous research linking drought to birch wood decline, highlighting its sensitivity to water-related issues. Oak: fungal and insect factors could decrease wood production by up to 0.1%. Prior studies emphasize the significant influence of fungal diseases on oak health and regeneration, as well as the impact of insect infestation on wood production. Alder: water-related factors may lead to a slight reduction in wood production, approximately 0.02%. The impact is significant within specific age ranges, indicating potential effects on harvesting. Pine: water- and climate-related factors may result in up to a 0.05% reduction in wood production. Pine, a key forest-forming species in Poland, is notably sensitive to these factors, especially as it nears harvesting age. Spruce: insects, fungi, and climate-related factors could lead to a reduction in wood production of up to 0.2%–0.3%. Analyses demonstrate sensitivity, resulting in a noticeable growth differential compared to the typical rate. Short-term predictions based on neural networks were developed, acknowledging their suitability for short-term forecasts due to uncertainties regarding long-term factor impacts. Additionally, our study discussed modeling wood increments in divisions well below the harvesting time, emphasizing that the influence of current and 2023–2025 factors on wood increments and supply may only manifest several decades from now. These results imply important indications for the economic and financial performance of the wood industry. Full article

This preliminary study seeks to determine the effect of R&P denervation on tumor growth and survival in immunocompetent rats bearing an aggressive and metastatic breast solid tumor. A novel microsurgical approach was applied “in situ”, aiming to induce R&P denervation through the division of every single nerve fiber connecting the host with the primary tumor via its complete detachment and re-attachment, by resecting and reconnecting its supplying artery and vein (anastomosis). This preparation, known as microsurgical graft or flap, is radically denervated by definition, but also effectively delays or even impedes the return of innervation for a significant period of time, thus creating a critical and therapeutic time window. Mammary adenocarcinoma cells (HH-16.cl4) were injected into immunocompetent Sprague Dawley adult rats. When the tumors reached a certain volume, the subjects entered the study. The primary tumor, including a substantial amount of peritumoral tissue, was surgically isolated on a dominant artery and vein, which was resected and reconnected using a surgical microscope (orthotopic tumor auto-transplantation). Intending to simulate metastasis, two or three tumors were simultaneously implanted and only one was treated, using the surgical technique described herein. Primary tumor regression was observed in all of the microsurgically treated subjects, associated with a potent systemic anticancer effect and prolonged survival. In stark contrast, the subjects received a close to identical surgical operation; however, with the intact neurovascular connection, they did not achieve the therapeutic result. Animals bearing multiple tumors and receiving the same treatment in only one tumor exhibited regression in both the “primary” and remote- untreated tumors at a clinically significant percentage, with regression occurring in more than half of the treated subjects. A novel therapeutic approach is presented, which induces the permanent regression of primary and, notably, remote tumors, as well as, evidently, the naturally occurring metastatic lesions, at a high rate. This strategy is aligned with the impetus that comes from the current translational research data, focusing on the abrogation of the neuro–tumoral interaction as an alternative treatment strategy. More data regarding the clinical significance of this are expected to come up from a pilot clinical trial that is ongoing. Full article

Hydractinia symbiolongicarpus is a colonial hydrozoan that displays a division of labor through morphologically distinct and functionally specialized polyp types. As with all cnidarians, their venoms are housed in nematocysts, which are scattered across an individual. Here, we investigate the spatial distribution of a specific protein family, jellyfish toxins, in which multiple paralogs are differentially expressed across the functionally specialized polyps. Jellyfish toxins (JFTs) are known pore-forming toxins in the venoms of medically relevant species such as box jellyfish (class Cubozoa), but their role in other medusozoan venoms is less clear. Utilizing a publicly available single-cell dataset, we confirmed that four distinct H. symbiolongicarpus JFT paralogs are expressed in nematocyst-associated clusters, supporting these as true venom components in H. symbiolongicarpus. In situ hybridization and immunohistochemistry were used to localize the expression of these JFTs across the colony. These expression patterns, in conjunction with known nematocyst type distributions, suggest that two of these JFTs, HsymJFT1c-I and HsymJFT1c-II, are localized to specific types of nematocysts. We further interpret JFT expression patterns in the context of known regions of nematogenesis and differential rates of nematocyst turnover. Overall, we show that JFT expression patterns in H. symbiolongicarpus are consistent with the subfunctionalization of JFT paralogs across a partitioned venom system within the colony, such that each JFT is expressed within a specific set of functionally distinct polyp types and, in some cases, specific nematocyst types. Full article

The cell wall, serving as the exoskeleton of plants, is naturally a barrier to resist external stresses. Protoplasts can be obtained by dissolving the cell walls of plant cells without damaging the cell membrane, and are widely used in the rapid propagation, transgenic breeding, and somatic hybridization of plants. However, to regenerate the cell wall is a precondition for cell division. Therefore, to study the culture condition and influencing factors during the cell wall regeneration of protoplasts is vital. Traditionally, culture medium is used to cultivate protoplasts, but it has some disadvantages. Herein, a microfluidic system with crossed channels was constructed to isolate and cultivate the protoplasts of tobacco. Then, the cell wall regeneration of the tobacco protoplasts was also studied based on this microfluidic system. It was found that, compared with the control, benzo-(1, 2, 3)-thiadiazole-7-carbothioic acid S-methyl ester (BTH) could accelerate the regeneration of the cell wall, while Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) could inhibit the regeneration of the cell wall within 24 h. To conclude, this study demonstrated that a crossed microfluidic chip could be an effective tool to study cell wall regeneration or other behavior of plant cells in situ with high resolution. In addition, this study revealed the rate of cell wall regeneration under BTH and Pst DC3000 treatment. Full article

Soil moisture is a key state variable in land surface processes. Since field measurements of soil moisture are generally sparse and remote sensing is limited in terms of observation depth, land surface model simulations are usually used to continuously obtain soil moisture data in time and space. Therefore, it is crucial to evaluate the performance of models that simulate soil moisture under various land surface conditions. In this work, we evaluated and compared two land surface models, the Common Land Model version 2014 (CoLM2014) and the Community Land Model Version 5 (CLM5), using in situ soil moisture observations from the Soil Climate Analysis Network (SCAN). The meteorological and soil attribute data used to drive the models were obtained from SCAN station observations, as were the soil moisture data used to validate the simulation results. The validation results revealed that the correlation coefficients between the simulations by CLM5 (0.38) and observations are generally higher than those by CoLM2014 (0.11), especially in shallow soil (0–0.1016 m). The simulation results by CoLM2014 have smaller bias than those by CLM5 . Both models could simulate diurnal and seasonal variations of soil moisture at seven sites, but we found a large bias, which may be due to the two models’ representation of infiltration and lateral flow processes. The bias of the simulated infiltration rate can affect the soil moisture simulation, and the lack of a lateral flow scheme can affect the models’ division of saturated and unsaturated areas within the soil column. The parameterization schemes in land surface models still need to be improved, especially for soil simulations at small scales. Full article

Atmospheric turbulence threatens flight safety of civil aviation aircraft by inducing aircraft bumpiness. A severity estimation method of aircraft bumpiness in turbulent flight is explored according to in-situ Eddy Dissipation Rate (EDR) indicator. With the turbulence intensity derived from EDR value, a time series of longitudinal and vertical turbulence was generated according to von Karman turbulence model. In order to obtain the vertical acceleration response of aircraft, the continuous change of aerodynamic force on the assembly of wing and horizontal tail was computed by Unsteady Vortex Lattice Method (UVLM). The computing accuracy was improved by using semi-circle division and assigning the vortex rings on the mean camber surface. Furthermore, the adverse effects of control surface deflections on bumpiness severity estimation can be effectively removed by separating turbulence-induced and aircraft maneuvers-induced aerodynamic force change. After that, the variance of vertical acceleration, as the severity indicator of aircraft bumpiness, was obtained by Welch spectrum estimation. With the refined grid level, the pitching moment change due to control surface deflections can be solved accurately by UVLM. The instantaneous acceleration change obtained by UVLM approximates recorded acceleration data with better accuracy than linear transfer function model. A further test with a set of flight data on the same airway shows that compared with in-situ EDR indicator, the proposed method gives an aircraft-dependent estimation of bumpiness severity, which can not only be used to estimate in-situ bumpiness but also be applied to forecast the bumpiness severity of other different aircrafts. Full article

Phalacroma rotundatum is a rare cosmopolitan heterotrophic dinoflagellate. This species, included in the IOC-UNESCO Taxonomic Reference List of Harmful Microalgae, may be a diarrhetic shellfish poisoning (DSP) toxin vector, but little is known about its ecophysiology and behavior. A vertical net haul collected during the austral summer of 2018 in Reloncaví Sound (Chilean Patagonia) revealed an unusually abundant population of P. rotundatum and prompted intensive 24 h sampling on 16–17 January to study the cell cycle and feeding behavior of this species. Hydrographic measurements from a buoy revealed the local characteristic estuarine circulation, with a brackish surface layer (salinity 26–28) separated from saltier, colder bottom waters by a pycnocline at a depth modulated by the tidal regime. A high proportion of P. rotundatum cells were packed with digestive vacuoles (peak of 70% at 14:00), and phased cell division (µ = 0.46 d⁻¹) occurred 3 h after sunrise. The division time (T_D) was 2 h. This is the first cell cycle study of P. rotundatum. The results here disagree with those of previous field studies that considered asynchronous division in some Dinophysis species to be related to heterotrophic feeding. They also question the very specific prey requirements, Tiarina fusus, reported for P. rotundatum in northern Europe. Full article

Escherichia coli is the primary cause of urinary tract infection (UTI), which is one of the most frequent human infections. While much is understood about the virulence factors utilized by uropathogenic E. coli (UPEC), less is known about the bacterial growth dynamics taking place during infection. Bacterial growth is considered essential for successful host colonization and infection, and most antibiotics in clinical use depend on active bacterial growth to exert their effect. However, a means to measure the in situ bacterial growth rate during infection has been lacking. Due to faithful coordination between chromosome replication and cell growth and division in E. coli, chromosome replication provides a quantitative measure of the bacterial growth rate. In this study, we explored the potential for inferring in situ bacterial growth rate from a single urine sample in patients with E. coli bacteriuria by differential genome quantification (ori:ter) performed by quantitative PCR. We found active bacterial growth in almost all samples. However, this occurs with day-to-day and inter-patient variability. Our observations indicate that chromosome replication provides not only a robust measure of bacterial growth rate, but it can also be used as a means to evaluate antibiotic effect. Full article

Stimuli-responsive systems for controlled drug release have been extensively explored in recent years. In this work, we developed a reduction-responsive camptothecin (CPT) nanocapsule (CPT-NC) by combining nanoprecipitation and in situ polymerization using a polymerized surface ligand and a disulfide bond-containing crosslinker. Dissolution rate studies proved that the CPT-NCs have robust drug-release profiles in the presence of glutathione (GSH) owing to the division of the disulfide bond crosslinker which triggers the collapse of the polymer layer. Furthermore, the in vitro investigations demonstrated that the CPT-NCs exhibited a high-cellular uptake efficiency and cytotoxicity for cancer cells of squamous cell carcinoma (SCC-15). Our approach thus presents an effective intracellular drug delivery strategy for anticancer therapy. Full article