Search Results (66)

Industrial hemp (Cannabis sativa L.) is gaining renewed interest as a sustainable source of natural fibres, particularly in regions like Belgium, where well-established flax processing infrastructure exists. However, region-specific data on varietal performance for long fibre production remain limited, hindering large scale adoption by both farmers and processors. This study aimed to assess the agronomic performance of early- and late-flowering hemp varieties under temperate maritime conditions through a three-year field trial at two sites in Flanders (Belgium). The evaluation focused on key parameters including emergence rate, plant morphology, long fibre yield and quality. Results showed that successful crop establishment is critical, as poor emergence influenced stem diameter (increasing with decreasing emergence) and leads to reduced long fibre yields by up to 50% between tested sites. Significant yield differences between trial years were present at both sites, with the biomass yield ranging from 10.7 to 14.5 and from 7.8 to 9.6 t ha⁻¹ for Bottelare and Beitem, respectively. Under favourable conditions, long fibre yields reached up to 2.4 t ha⁻¹ for late-flowering and 2.1 t ha⁻¹ for early- and mid-late-flowering varieties. In Western Europe, early to mid-late flowering varieties are generally favoured. Among these, the mid-late flowering variety Bialobrzeskie demonstrated strong yield potential. Overall, fibre quality across varieties was comparable to that of flax. Full article

The roles of endophytes in Cannabis sativa (cannabis, hemp) remain poorly explored. While in vitro studies suggest that there can be several benefits, such as plant growth promotion and protection against pathogens, more in planta studies are needed. This review summarizes the bacterial and fungal endophytes previously reported in tissues of C. sativa and discusses the factors influencing their presence, as well as their potential beneficial and detrimental effects. Using genome sequencing and culture-based approaches, we describe the microbial diversity in hydroponically cultivated cannabis plants at several developmental stages. These include mother plants, cuttings, vegetative and flowering plants, and tissue-cultured plantlets. Microbes that were present include fungal, yeast, and bacterial endophytes found in roots, stems, leaves, inflorescences, and seeds. These may have originated from the growing substrate or be transmitted through vegetative propagation. Notable endophytes included Rhizophagus irregularis (a mycorrhizal fungus), Penicillium chrysogenum (an antibiotic producer), and various endophytic yeast species not previously described in C. sativa. Endophytes representing potential plant pathogens, such as Fusarium oxysporum, are also present within cannabis tissues, which can negatively impact plant health. Using scanning electron microscopy, we observed that fungal propagules are present within pith parenchyma cells and xylem vessel elements in stem tissues, illustrating for the first time the in situ localization and distribution of endophytes in cannabis vascular tissues. The mechanism of spread through xylem vessels likely contributes to the spread of endophytes within cannabis and hemp plants. Further research is required to validate the roles of endophytes in cannabis and hemp plants grown under commercial production conditions. Full article

This study investigated efficient extraction methods for cannabinoids and terpenes from the above-ground parts of Futura 75, focusing on two techniques: pressurized extraction and magnetic stirrer-assisted extraction. The effects of solvent type, temperature, time, and pressure were evaluated using five organic solvents and two binary solvent systems. Cannabinoid profiles of obtained extracts were analyzed using gas chromatography coupled with mass spectrometry (GC-MS), while terpene profiles were characterized through solid-phase microextraction (SPME) combined with GC-MS. Next, two selected extracts with the highest content of cannabinoid and terpene fractions (Futu1 and Futu2) were tested for antiproliferative activity toward cancer cell lines (MV4-11, AGS, HT-29, MDA-MB-468, MCF-7) and their cytotoxicity was evaluated on non-tumorigenic MCF-10A cells. Extract Futu1 contained 51.57% cannabinoids, 9.8% monoterpenes, and 90.2% sesquiterpenes in the terpene fraction. Futu2 exhibited a higher proportion of monoterpenes in the terpene fraction (19.6% monoterpenes and 80.4% sesquiterpenes) and consisted of 49.49% cannabinoids. Both extracts exhibited higher selectivity for cancer cells over non-tumorigenic cells, with Futu2 demonstrating stronger antiproliferative properties. Interestingly, lower concentrations of extracts and tested free, single cannabinoids stimulated the growth of leukemia (MV4-11) and breast cancer (MDA-MB-468) cell lines while their higher concentrations suppressed proliferation. Full article

In this work, two extraction techniques, conventional and ultrasound-assisted extraction (UAE) techniques, were employed for the extraction of natural bioactive compounds (NBCs) from the areal parts of industrial hemp (Cannabis sativa L. cv. Helena) at two harvesting stages: (i) the beginning of flowering and (ii) the full flowering of the hemp plants. In the conventional extraction, the effect of different extraction solvents on the extraction yield and the content of NBCs was examined. The extraction temperature, extraction time, and ultrasonic power were chosen for the process parameters in UAE. The highest value of the investigated responses in UAE-obtained extracts was higher compared to extract obtained with conventional extraction techniques when the same solvent was used (50% ethanol): extraction yield (17.54 compared to 15.28%), content of total phenols and total flavonoids (1.7795 compared to 1.0476 mg GAE/mL and 0.6749 compared to 0.3564 mg CE/mL, respectively) and cannabidiol (0.8752 compared to 0.4310 mg/mL). Comparing the plant material in different developmental stages, it can be concluded that hemp aerial parts at the beginning of the flowering stage represent a good source of the phenolic compound with sinapic acid and apigenin being dominant, while hemp aerial parts in the full flowering stage represent a good source of cannabinoids. Full article

Industrial hemp (Cannabis sativa L.) is a versatile and sustainable multipurpose plant for agroecology services and a zero-waste circular economy. While the focus has traditionally been on primary products like fiber and seeds, nowadays there is an increasing awareness of the potential value of the by-products generated during hemp cultivation and processing. This article explores various methods of valorizing industrial hemp wastes, focusing on their mineral and biochemical composition, highlighting the benefits of utilizing what was once considered a mere by-product. The apical and the basal leaves of 12 industrial hemp varieties, six monoecious, and six dioecious, representing the main by-product of fiber supply chain, were assessed for their mineral (N, K, Na, Ca; Mg, Cu, Mn, Fe, and Zn), chlorophyll, carotenoids, and total soluble phenols contents, as well as for their antioxidant activity. The same parameters were also evaluated in the inflorescences; the main waste was derived from both hemp fiber and seed harvesting, which were collected at three stages of flower development for four selected genotypes, together with the yield and chemical composition of their essential oils. Differences in the evaluated parameters among genotypes and tissues were highlighted, showing the potential for diversifying the utilization of industrial hemp wastes. The possible uses of these residual biomasses are discussed based on their composition. Full article

Industrial hemp (Cannabis sativa L.) is primarily a dioecious plant, and monoecious varieties have been developed for high yield. Production practices vary for each variety, prompting the need for the present study to describe the floral characteristics and evaluate pollen quantity and viability of monoecious and dioecious hemp varieties. All four hemp varieties, Henola, CFX-2, Canda, and Joey, have five lanceolate anthers, basifixed to a threadlike filament. Anther length was significantly different among varieties, but not anther width. The longest length (0.38 ± 0.046 cm) was recorded in Henola, and the shortest (0.34 ± 0.043 cm) in CFX-2. Anther width ranged from 0.088 ± 0.0024 to 0.095 ± 0.0021 cm. Pollen grains were triporate and spheroidal in shape and size and differed significantly, with the largest in Joey (27.83 ± 0.78 μm) and Henola (27.489 ± 0.99 μm), and smallest in Canda (22.04 ± 0.56 μm). The number of pollen grains per flower differed significantly among varieties, ranging from 29,183 in Henola to 104,548 in Joey. Even though Henola recorded the lowest pollen number, it had the highest percentage (69.3%) of viable pollen after 24 h of storage 4 °C and Canda had the lowest (54%). Three weeks after storage at the same temperature, pollen viability decreased for all the hemp varieties and ranged from 52% to 58%. There was a moderate, positive and significant relationship (r = 0.496) between anther length and the number of pollen grains in Joey. The relationship in Henola was moderate and non-significant (r = 0.356), and it was weak and non-significant in Canda (r = 0.188) and in CFX-2 (r = 0.037). The findings from this study provide information for growers and researchers on hemp breeding and cultivation practices that may contribute to the prevention of cross-pollination. Full article

The growing interest in Cannabis sativa as a highly used crop is present worldwide. There are limited data about the effect of potassium (K) fertilizer on industrial hemp yield for dual purposes (seed and stem production). The current study aimed to investigate the influence of adding two different K fertilizers, KCl and K₂SO₄, at two growth stages (flowering and ripening) on the productivity and chlorophyll a fluorescence (ChlF) of Cannabis sativa, variety Finola. Before sowing, different K treatments were applied: K₁—100 kg ha⁻¹ KCl (60% K) and K₂—100 kg ha⁻¹ K₂SO₄ (52% K, S 17%). The OJIP (O stands for “origin” (minimal fluorescence), P for “peak” (maximum fluorescence), and J and I for inflection points between the O and P levels) data were recorded and used for ChlF transients and individual ChlF parameters during vegetation. At harvest, the stem morphology parameters and yield (plant height, stem weight and diameter, and stem and seed yield), tensile strength, and the modulus of elasticity were determined. The results show the sensitivity of minimal (F₀) and maximal fluorescence (F_m), electron transport from Q_A to intersystem electron acceptors (ET₀/(TR₀ − ET₀)), and electron transport flux until PSI acceptors (RE₀/RC) to K fertilization. The parameters that described electron transport (ET₀/RC, ψE_0, and φE₀), performance index on absorption basis (PI_ABS, TR₀/DI₀, and φP₀), dissipation (DI₀/RC), and electron transport to photosystem I (φR₀ and δR₀/(1 − δR₀)) had a reaction only at the growth stage, indicating a change in their activity during the aging of the Cannabis sativa plants. The average stem height was 67.5 cm, and the stem diameter was 0.41 cm. The different K sources did not significantly influence the stem height and diameter, nor the dry stem (on average 12.2 t ha⁻¹) and seed yield (on average 1.85 t ha⁻¹). The tensile strength of individual hemp stems was the highest with K₂SO₄ (53.32 MPa) and the lowest with KCl (49.25 MPa). The stem stiffness by modulus of elasticity was about 5 GPa on average for all the treatments. In general, the photosynthetic parameters in this study varied more between the growth stages than between the different K fertilizer formulations. Moreover, based on the results of this study, it can be recommended to use both fertilizers, KCl and K₂SO₄, in dual-purpose industrial hemp production since no significant effect was found for the stem morphometric and biomechanical parameters as well as for the agronomic parameters. Full article

Hemp (Cannabis sativa L.), one of the earliest domesticated crops, has diverse applications in textiles, construction, nutrition, and medicine. Breeding advancements, including speed breeding, accelerate genetic improvements in crops by optimizing environmental conditions for reduced generation times. This study employed greenhouse and field experiments to develop a proprietary yellow-stemmed hemp germplasm with a unique stem trait. Initial crossbreeding between the late Eletta Campana (medium green stems) and the early Chamaeleon (yellow stems) demonstrated the recessive monogenic inheritance of the yellow-stem trait and fast and safe stabilization even in the case of parent varieties with different flowering times. Controlled flowering in the case of photoperiod-sensitive genotypes, manual pollination, and successive backcrossing stabilized the yellow-stem trait over six cycles, with 100% trait consistency achieved by the fifth cycle within just 12 months in total. Open-field trials validated greenhouse results, showing strong correlations between visual stem color assessments and visible atmospherically resistant index (VARI) obtained through remote sensing imagery. Cannabinoid analyses indicated significant reductions in tetrahydrocannabinol (THC) content while maintaining optimal cannabidiol (CBD) levels. Accumulated growing degree days (GDDs) optimized flowering and maturity, ensuring consistency in phenological traits. This research highlights the utility of speed breeding and chemical analysis to accelerate trait stabilization and improve industrial hemp’s agronomic potential for fiber and CBD production while adhering to regulatory THC limits. Full article

Phytochemical diversity is increasingly appreciated as an important attribute of plants that affects their interactions with other organisms and can have substantial effects on arthropod communities, but this axis of diversity is less studied for agricultural plants. For both managed and natural systems, understanding how extreme weather events, such as droughts, floods, and extreme wind, affect phytochemical diversity is an important part of predicting responses of plant–arthropod interactions to climate change. In an outdoor field experiment with two distinct varieties of hemp (Cannabis sativa L., Cannabaceae), we investigated the effects of simulated water stress from reduced water availability and flooding, along with an unplanned extreme wind event on phytochemical diversity and cannabinoid profiles. We also examined how changes in chemistry affected the diversity of the associated arthropods. Our results indicate that both genetic variety and environmental stress have substantial effects on variation in hemp phytochemical diversity and cannabinoid composition, and these effects cascaded to alter the arthropod communities on flowers. The largest differences in chemistry were found between different varieties, which accounted for over 10% of the variation in phytochemical diversity. Stress from wind and floods reduced the phytochemical diversity of flowers, wind had negative effects on cannabidiol (CBD) concentrations, and both water deficit and flooding caused subtle shifts in cannabinoid composition. The subsequent cascading effects of chemistry depended on how it was characterized, with increases in CBD causing higher arthropod richness, while increased phytochemical diversity reduced arthropod diversity. These results provide insights into the potential effects of extreme weather on hemp chemistry, as well as the consequences of hemp phytochemical diversity on colonizing arthropods. Full article

In Europe, industrial hemp (Cannabis sativa L.) is currently cultivated primarily for its fibers and seeds due to regulatory restrictions on flower use, although the flowers present untapped valorization potential. This study assesses the economic viability of cultivating hemp for its inflorescences and examines the quality impact of different cultivation methods by testing two varieties, Santhica 27 and Félina 32, across distinct growth environments. The cultivation systems tested include greenhouse-based soilless setup and open-field production. Within the greenhouse, the effects of an ethylene-based growth regulator, applied during the vegetative stage, on plant morphology and cannabinoid concentrations were assessed at three dosage levels (7.5, 15, and 30 µL L⁻¹). The results showed a dose-dependent reduction in shoot height, lasting 21 to 28 days post-treatment before diminishing. Comparable total cannabigerol (CBG) levels were achieved with Santhica 27 in both field conditions and at the 30 µL L⁻¹ ethylene dose in the greenhouse, while total cannabidiol (CBD) levels in Félina 32 were higher in the field than in the greenhouse. Overall, greenhouse cultivation yielded significantly greater cannabinoid production per square meter due to increased inflorescence biomass and the potential for multiple harvest cycles. However, operating costs for greenhouse cultivation are at least 13 to 15 times higher than those of open-field production. Economic analysis suggests that optimizing greenhouse cultivation techniques and reducing labor requirements at harvest could help mitigate these costs, enhancing the economic feasibility of greenhouse-based hemp flower production. Full article

Here, we examine the effects of ploidy variation in Cannabis sativa L. cell lines on the plant host genotype-associated microbiome. The endophytic microbiome has a protocooperative role in improving plant health and productivity and represents an alternative to synthetic chemical fertilizers and pesticides in sustainable agriculture. This study assessed the effects of seed endophytes on diploid and triploid Haze hemp cultivars. Key phenotypic characteristics were evaluated, revealing significant differences in seed germination in vitro as well as vegetative growth and flowering in phytotron conditions. Endophyte-treated triploid plants exhibited significantly taller heights compared to diploids (p < 0.01). These treated triploid plants also showed longer leaves at nodes 2, 6, and 8, except at node 4, indicating a plant in transition from vegetative growth to the generative developmental stage. Additionally, triploids treated with endophytes displayed the highest number of axillary branches, while endophyte-treated diploids had the fewest (p < 0.05). Both cultivars treated with endophytes exhibited a higher number of inflorescences compared to untreated control plants. This study revealed for the first time a direct correlation between the shifts in diameter of the stem and the biomass in both tested hemp hosts, in association with endophytic microbiomes. Full article

Crotalaria juncea (sunn hemp) is a tropical forage legume used as a cover, forage, and fiber crop. Sunn hemp seed production occurs primarily in India because it requires short days to flower and set seed. Seeds available for production are typically non-specific genotypes instead of true breeding varieties. As sunn hemp is grown in more locations, understanding not only its performance in different growing conditions but also variations in genotype performance is critical for production management. We evaluated the growth and biomass accumulation of four genotypes (KMB1, KMB2, Thailand Original Sunn, and ‘Tropic Sunn’) of sunn hemp grown in northern semi-arid and humid-continental environments, Wyoming (Adams ‘22 and ‘23 (irrigated), Wyarno ‘23 (rainfed)) and Minnesota (Morris ‘22 and ‘23), USA. Thailand Original Sunn had the fastest growth rate (height over time) but the slowest canopy closure (NDVI over time), while KMB1 had the slowest growth rate but the fastest canopy closure. While growth rates varied among sunn hemp germplasm, there were no marked differences in biomass accumulation when harvested at 60 and 90 days after planting. Although the genotype did not have a significant effect on biomass accumulation, the environment affected not only growth but also biomass accumulation. At 60 DAP, the sunn hemp biomass averaged 1836, 489, 2459, 3334, and 731 kg ha⁻¹ in the Adams ‘22, Adams ‘23, Morris ‘22, Morris ‘23, and Wyarno ‘23 environments, respectively. At 90 DAP, the sunn hemp biomass averaged 6459, 4573, 7979, 7403, and 2220 kg ha⁻¹ in the Adams ‘22, Adams ‘23, Morris ‘22, Morris ‘23, and Wyarno ‘23 environments, respectively. The growth rate, canopy closure, and biomass accumulation differed when compared between the semi-arid environments and the humid-continental environment, with the humid-continental environment producing faster growth and higher biomass. These findings support the hypothesis that genotypes are likely to perform as predicted within growing regions, but there may be room to improve performance in different environments through selective breeding. Full article

Light is crucial for higher plants, driving photosynthesis and serving as a powerful sensory signal that profoundly modulates growth, development, physiological functions, hormone activation, and biochemical pathways. Various light parameters—quality, intensity, composition, and photoperiod—exert a tremendous influence on plant growth and development, particularly in industrial hemp (Cannabis sativa L.). C. sativa, a crop of historical significance and unparalleled versatility, holds immense value in the food, fiber, and medicinal industries. The cultivation of medicinal cannabis is burgeoning in controlled environments due to evolving healthcare regulations. Optimal light conditions significantly enhance both yield and harvest quality, notably increasing the density of apical inflorescences and the ratio of inflorescence to total aboveground biomass. C. sativa metabolites, especially phenolic and terpene compounds and Phytocannabinoids like CBD (cannabidiol), THC (tetrahydrocannabinol), and CBG (cannabigerol), possess immense medicinal value. Secondary metabolites in C. sativa predominantly accumulate in the trichomes of female flowers and surrounding sugar leaves, underscoring the critical need to boost inflorescence weight and metabolite concentrations while ensuring product consistency. Different light parameters distinctly impact C. sativa’s metabolic profile, providing a robust foundation for understanding the optimal conditions for synthesizing specific secondary metabolites. While the effects of light measurement on various crops are well-established, scientific evidence specifically relating to light quality effects on C. sativa morphology and secondary metabolite accumulation remains scarce. In this review, we critically summarized how different light properties can alter cannabis growth (vegetative and reproductive), physiology and metabolism. Furthermore, the mechanisms by which specific wavelengths influence growth, development, and secondary metabolite biosynthesis in C. sativa are not fully elucidated, which could be a prospective task for future researchers. Our review paves the way for a profound understanding of light’s influence on C. sativa growth and advancements in greenhouse settings to maximize metabolite production for commercial use. Full article

The purpose of this study was to evaluate the feasibility of using residues from cooking oil production to produce alternative biochar fuels along with optimizing the pyrolysis process. The work consisted of carrying out the pyrolysis process at varying temperatures and holding times at the final temperature, and then evaluating the energy potential of the materials studied. Taking into account aspects of environmental emissions, the content of selected oxides in the flue gases generated during the combustion of cakes and the biochar obtained from them was evaluated. Plant biomass derived from a variety of oilseeds, i.e., fennel flower (Nigella sativa L.), rapeseed (Brassica napus L. var. Napus), flax (Linum usitatissimum L.), evening primrose (Oenothera biennis L.), milk thistle (Silybum marianum L. Gaertn.) and hemp (Cannabis sativa L.), was used to produce biochar. The experimental data have shown that the obtained biochar can have a calorific value of nearly 27 MJ kg⁻¹. The use of pyrolysis allowed for a maximum increase in the calorific value of nearly 41% compared to non-thermally processed cakes and a several-fold decrease in carbon monoxide, nitrogen oxides and sulfur dioxide emissions. According to these results, it can be concluded that the pyrolysis process can be an attractive method for using residues from the production of various cooking oils to produce alternative biofuels, developing the potential of the circular economy. Full article

Hemp (Cannabis sativa L.) is a rapidly growing plant with multipurpose uses, and the optimal combination of yield and quality of hemp products (fibers, inflorescences, or seeds) may provide economic opportunities to uncover the full spectrum of its capabilities. The presented experimentation took place over seven years (2017–2023) in Greek climatic conditions for fourteen (14) registered monoecious and dioecious varieties. It can be concluded that the production of biomass, fiber, and seed weight were different not only between varieties but also from year to year. Despite significant variation between harvest years in biomass yield, the extracted fiber was relatively constant. Moreover, not only the dioecious varieties but also the monecious varieties could be effectively grown for biomass production in Greece. Regarding fiber production, monoecious varieties had the highest yields, apart from the dioecious Kompolti variety, which was the most fiber-productive. Under the experimentation conditions, early flowering varieties were most suited for seed production, and the KC Dora variety produced the heavier seeds. Generally, the Futura 75 variety was one of the most productive varieties for biomass and seed weight, while the Bialobrzeskie variety produced the greatest amounts of fiber. Full article