Search Results (80)

The upper facilities of polar marine equipment face severe freezing risks in ice-covered regions, necessitating energy-efficient electric heat tracing design. Existing models neglect coupled environmental factors (temperature–wind–humidity), leading to the overestimation of heating power. In this paper, experiment and CFD simulation are used to study the change of convective heat transfer coefficients of electric tracing circular tube components under the polar coupling environmental conditions of wind speed of 0~8 m/s, temperature of −40~0 °C, and air relative humidity of 10~95%, and the corresponding mathematical prediction model is established. The results show that increasing the wind speed and relative humidity will both increase the convective heat transfer coefficient of the circular tube, while the temperature is inversely proportional to the convective heat transfer coefficient of the circular tube. The convective heat transfer coefficient shows an average growth rate of only 2.8–3.8% as the temperature decreases from −10 °C to −40 °C, which is significantly lower than the effects of wind speed (average growth rate 59–50%) and humidity (average growth rate 7.5–12.7%). When the wind speed exceeds 2 m/s, the growth rate of humidity’s effect on the coefficient increases from 17.82% to 33.96%. Mathematical prediction models can provide certain references for the calculation and design of reasonable heating amounts for anti-icing and de-icing of polar equipment’s circular tube components under ice-covered regions. Full article

The study investigated the sperm motility and morphometry of pre-freeze and post-thaw boar epididymal semen cooled at increasing holding times at 18 °C. A total of 50 testes of heterogeneous boars were collected (5 testes/day) from the local abattoir and transported to the laboratory at 5 °C within 30 min after slaughter. Semen was retrieved from the caudal part of the epididymis using the slicing float-up method, diluted with Beltsville Thawing Solution extender, pooled in a 50 mL centrifuge tube/5 testes/day, and cooled at 18 °C. Following each holding time (0, 3, 6, 9, 12, 24, and 48 h), the cooled semen sample was re-suspended with Fraction A extender and stored at 5 °C for an additional 45 min. A cooled resuspended semen sample was then diluted with Fraction B extender, loaded into 0.25 mL straws, and frozen using liquid nitrogen vapour. Thawing was accomplished by immersing the semen straws in warm (37 °C) water for 1 min and the samples were evaluated for sperm motility and morphometry traits using the computer-assisted sperm analyzer system. The data were analyzed using variance analysis. Descriptive statistics were used to assess sperm morphometry, establishing the minimum and maximum values. Boar epididymal sperm survived for up to 48 h when held at 18 °C. Furthermore, the highest post-thawed sperm motility rates were observed in semen frozen after 3 h of holding time, with a sperm total motility of 85.9%, a progressive motility of 60.3%, and a rapid motility of 33.2%, as compared to other holding times (p < 0.05). The acceptable ranges for pre-freeze and post-thawed sperm morphology were head length (8.4–9.1 µm), width (4.4–4.8 µm), area (29.9–38.2 µm²), perimeter (20.1–23.7 µm), midpiece width (1.1–2.8 µm), and sperm shape, were consistent regardless of the holding time. A holding time of 3 h enhances the cryoresistance of sperm cooled at 18 °C. Therefore, these findings suggest that boar epididymal sperm can be effectively conserved and can maintain fertilization capability when cooled for 3 h at 18 °C before freezing. Full article

Human milk contains a variety of biologically active molecules that are essential for infant growth and development, as well as indicators of maternal health. However, understanding the full potential of these molecules is challenging due to variations in their concentrations among mothers, potential degradation during sample handling and storage, and the limited accessibility of specific human milk analyses. This study aimed to evaluate the effectiveness of a freeze-dried preservative cocktail in maintaining the stability of key milk molecules during collection, transport, and storage. GenV participants (n = 96) were given a sample collection kit and followed the instructions to collect approximately 5 mL of breast milk, which was placed in a collection tube containing the preservative. The samples were mailed at ambient temperature to the GenV laboratory (Murdoch Children’s Research Institute, Melbourne, Victoria, Australia), where they were aliquoted into 1 mL tubes using a liquid handling system (Janus) and stored at −80 °C. These samples were randomly selected and sent to The University of Western Australia (Perth, Western Australia, Australia) on dry ice for biochemical analysis. The average collection day postpartum was 16 ± 14 (range 1–91 days), while the average postal receipt time was 5 ± 3 days (range 1–16 days), and samples were processed within 6 days of receipt (average 3 ± 2 days). The mean concentrations of key molecules—fat (48.6 ± 17.1 g/L), protein (15.5 ± 4.3 g/L), lactose (78.9 ± 13.9 g/L), glucose (0.17 ± 0.17 g/L), lysozyme (0.16 ± 0.16 g/L), and insulin (6.1 ± 4.9 μIU/mL)—were consistent with reported literature values. There were no statistically significant differences in molecular concentrations based on postal transit time, receipt, or processing delays (p > 0.05). These results demonstrate that the preservative cocktail effectively preserved the integrity of key molecules in human milk during handling, postal transport, and storage at ambient temperature. The findings support its use as a valuable tool for human milk research, enabling more flexible sample collection and handling without compromising the quality of the milk or the biochemical analysis. Future research should explore its application in broader contexts to further enhance the accuracy and reliability of milk composition studies across diverse research settings. Full article

It is known that ultrashort echo time (UTE) magnetic resonance imaging (MRI) sequences can detect signals from water protons but not collagen protons in short T2 species such as cortical bone and tendons. However, whether collagen protons are visible with the zero echo time (ZTE) MRI sequence is still unclear. In this study, we investigated the potential of the ZTE MRI sequence on a clinical 3T scanner to directly image collagen protons via D₂O exchange and freeze-drying experiments. ZTE and UTE MRI sequences were employed to image fully hydrated bovine cortical bone (n = 10) and human patellar tendon (n = 1) specimens. Then, each specimen was kept in a 30 mL syringe filled with D₂O solution for two days. Fresh D₂O was flushed every 2 h to reach a more complete D₂O–H₂O exchange. Later, the samples were lyophilized for over 40 h and then sealed in tubes. Finally, the samples were brought to room temperature and visualized using the identical 3D ZTE and UTE sequences. All hydrated bone and tendon specimens showed high signals with ZTE and UTE sequences. However, all specimens showed zero signal after the D₂O exchange and freeze-drying procedures. Therefore, similar to UTE imaging, the signal source in ZTE imaging is water. The ZTE sequence cannot directly detect signals from collagen protons in bone and tendons. Full article

Offshore wind power is a new type of clean energy with broad development prospects. Accurate analysis of the uplift capacity of offshore wind turbine foundations is a crucial prerequisite for ensuring the safe operation of wind turbines under complex hydrodynamic conditions. However, current research on the uplift capacity of suction caissons often neglects the high-sensitivity characteristics of marine soils. Therefore, this paper first employs the freeze–thaw cycling procedure to prepare high-sensitivity saturated clay. Subsequently, a single−tube foundation for wind turbines is constructed within a centrifuge through a penetration approach. Ten sets of centrifuge model tests with vertical cyclic pullout are conducted. Through comparative analysis, this study explores the pullout capacity and its variation patterns of suction caisson foundations in clay with different sensitivities under cyclic loading. This research indicates the following: (1) The preparation of high-sensitivity soil through the freeze−thaw procedure is reliable; (2) the uplift capacity of suction caissons in high−sensitivity soil rapidly decreases with increasing numbers of cyclic loads and then tends to stabilize. The cumulative displacement rate of suction caissons in high-sensitivity soil is fast, and the total number of pressure–pullout cycles required to reach non-cumulative displacement is significantly smaller than that in low-sensitivity soil; (3) the vertical cyclic loading times and stiffness evolution patterns of single-tube foundations, considering the influence of sensitivity, have been analyzed. It was found that the secant stiffness exhibits a logarithmic function relationship with both the number of cycles and sensitivity. The findings of this study provide assistance and support for the design of suction caissons in high-sensitivity soils. Full article

In order to study the development law of the irregular hole freezing temperature field, combined with the shield solution project of the Nanjing Water Supply Corridor, the distribution characteristics and influencing factors of the irregular freezing temperature field of the river bottom shield machine are studied by numerical simulation. The following conclusions are obtained: (1) The extension length of the outer ring pipe is correlated approximately positively with the thickness and average temperature of the freezing wall at the bottom of the cup. The thickness increases by 0.25 m, and the average temperature decreases by 1.25 °C for every 1 m increase in the extension length. (2) The intersection time decreases logarithmically with the increase in the extension length of the outer ring tube. (3) As the ratio of the axial angle between the two adjacent tubes in the weak area of the outer ring tube becomes larger, the temperature of the weak point in the center of the two tubes increases approximately linearly. The midpoint temperature of the two tubes increases by 3.3 °C for every 1 increase in the angle coefficient. (4) With the increase in the opening angle of the inner ring hole, the thickness and average temperature change, respectively, at 150 d are not more than 0.15 m and 0.6 °C. The results show that under the irregular freezing form, the angle and length of the outer ring pipe have a great influence on the temperature field, and the angle of the inner ring pipe has little influence on the final distribution of the temperature field. The average temperature and the temperature distribution of the weak points show a trend of decreasing first and then increasing along the shield advancing direction, reaching a minimum near the cutterhead. Full article

The regasification of liquefied natural gas (LNG) is a crucial process that involves certain challenges created by the low temperature of LNG and the risk of ice formation on the external surfaces of the tubes of heat exchangers, which can hinder heat transfer and increase flow resistance. This study presents a numerical model for ice formation on the external surface of the U-bend tube of shell-and-tube heat exchangers. The numerical model has been further enhanced by applying a custom user-defined function. The numerical results were validated using experimental data and demonstrated excellent predictive capability, particularly for the surface temperature of the tubes and the thickness of the ice layer. Hence, this model can reliably capture the overall behavior of the ice formation on the external surfaces of the tubes of shell-and-tube heat exchangers. By highlighting the importance of maintaining stable heat transfer conditions to prevent freezing, this study offers valuable insights that can guide the optimization of heat exchanger designs for LNG regasification. Full article

BACKGROUND: Platelet-rich plasma (PRP) formulations have become valuable therapeutic tools in regenerative medicine. In addition, these blood derivates have been successfully included in cell therapy as fetal bovine serum substitutes, due to the real need to avoid the risk of host immunologic reactions and the animal disease transmission associated with reagents from animal origin. However, the protocols for obtaining them should be optimized to improve their biological potential. METHODS: PRP-derived preparations with different concentrations of the platelet and plasma components were obtained from the blood of five donors by freeze-drying. Measurements of the pH, protein, and growth factor concentration were performed. Moreover, their biological effects on cell proliferation and migration and their angiogenic potential were assessed. RESULTS: An increased plasma component concentration resulted in an augmented quantity of the total protein content, a significative variation in the hepatocyte growth factor concentration, and an experimental but clinically irrelevant alteration of the pH value. No significant changes were induced in their potential to enhance proliferative and migratory responses in epithelial cells, with the latter being reduced for dermal fibroblasts. The endothelial cell capacity for tube formation was significatively reduced. CONCLUSIONS: An increased blood plasma content did not improve the biological potential of the formulations. However, they have emerged as a promising approach for regenerative therapies where neovascularization must be avoided. Full article

To face the challenges in preparing hydrogel fibers with complex structures and functions, this study utilized a microfluidic coaxial co-extrusion technique to successfully form functional hydrogel fibers through rapid ionic crosslinking. Functional hydrogel fibers with complex structures, including linear fibers, core–shell structure fibers, embedded helical channels, hollow tubes, and necklaces, were generated by adjusting the composition of internal and external phases. The characteristic parameters of the hydrogel fibers (inner and outer diameter, helix generation position, pitch, etc.) were achieved by adjusting the flow rate of the internal and external phases. As biocompatible materials, hydrogel fibers were endowed with electrical conductivity, temperature sensitivity, mechanical enhancement, and freeze resistance, allowing for their use as temperature sensors for human respiratory monitoring and other biomimetic application developments. The hydrogel fibers had a conductivity of up to 22.71 S/m, a response time to respiration of 37 ms, a recovery time of 1.956 s, and could improve the strength of respiration; the tensile strength at break up to 8.081 MPa, elongation at break up to 159%, and temperature coefficient of resistance (TCR) up to −13.080% °C⁻¹ were better than the existing related research. Full article

Discrepant sample processing remains a significant challenge within blood metabolomics research, introducing non-biological variation into the measured metabolome and biasing downstream results. Inconsistency during the pre-analytical phase can influence experimental processes, producing metabolome measurements that are non-representative of in vivo composition. To minimize variation, there is a need to create and adhere to standardized pre-analytical protocols for blood samples intended for use in metabolomics analyses. This will allow for reliable and reproducible findings within blood metabolomics research. In this review article, we provide an overview of the existing literature pertaining to pre-analytical factors that influence blood metabolite measurements. Pre-analytical factors including blood tube selection, pre- and post-processing time and temperature conditions, centrifugation conditions, freeze–thaw cycles, and long-term storage conditions are specifically discussed, with recommendations provided for best practices at each stage. Full article

To investigate the seismic behavior of composite columns with high-strength concrete-filled steel tube flanges and honeycomb steel webs (STHHC) after being subjected to freeze-thaw cycles, 36 full-scale STHHCs were designed with the following main parameters: the shear span ratio (λ_s), the axial compression ratio (n₀), the number of freeze-thaw cycles (N_c), the concrete cubic compression strength (f_cu), and the steel ratio of the section (α_s). Compared with existing experimental data, the validity of the finite element modeling method was verified. Parameter analysis was conducted on 36 full-scale STHHCs to obtain the hysteresis curve of the composite columns and to clarify the impact of the different parameters on the skeleton curve, the energy dissipation capacity, the stiffness degradation, and the ductility of the composite columns. The results showed that the hysteresis curves of all specimens after freeze-thaw cycles exhibited an ideal shuttle shape, reflecting that this kind of composite column has good energy dissipation ability and freeze-thaw resistance. The specimens’ maximum bulging deformation and maximum stress both occurred at the column base. Finally, the restoring force model of this kind of composite column is therefore established, and design recommendations based on these results are proposed. Full article

Conventional asphalt roads are noisy. Currently, there are two main types of mainstream noise-reducing pavements: pore acoustic absorption and damping noise reduction. However, a single noise reduction method has limited noise reduction capability, and porous noise-reducing pavements have a shorter service life. Therefore, this paper aimed to improve the noise-damping performance of porous asphalt mixture by adding rubber granules and extending its service life using electromagnetic induction heating self-healing technology. Porosity and permeability coefficient test, Cantabro test, immersion Marshall stability test, freeze–thaw splitting test, a low-temperature three-point bending experiment, and Hamburg wheel-tracking test were conducted to investigate the pavement performance and water permeability coefficients of the mixtures. A tire drop test and the standing-wave tube method were conducted to explore their noise reduction performance. Induction heating installation was carried out to study the heating rate and healing performance. The results indicated that the road performance of the porous asphalt mixture tends to reduce with an increasing dosage of rubber granules. The road performance is not up to the required standard when the dosage of rubber granules reaches 3%. The mixture’s performance of damping and noise tends to increase with the increase of rubber granule dosage. Asphalt mixtures with different rubber granule dosages have different noise absorption properties, and the mixture with 2% rubber granules has the best overall performance (a vibration attenuation coefficient of 7.752 and an average absorption factor of 0.457). The optimum healing temperature of the porous asphalt mixture containing rubber granules and steel wool fibers is 120 °C and the healing rate is 74.8% at a 2% rubber granule dosage. This paper provides valuable insights for improving the noise reduction performance and service life of porous asphalt pavements while meeting road performance standards. Full article

Stool is a mixture of excrement, microbiota, enzymes, undigested material, and small molecules. Fecal metabolomics has gained interest recently, owing to advances in metabolomics and growing research into both the host’s physiology and the gut microbiome. One challenge with fecal metabolomics is preserving the sample integrity from collection until analysis, as the microbiota and enzymes continue to alter the metabolome following defecation. Currently, flash-freezing or lyophilization are utilized to minimize post-collection metabolome changes; however, this requires complex equipment and immediate processing, precluding the possibility for at-home sampling. Commercial devices containing stabilizing solvents have been developed to facilitate at-home collection, ambient transport, and sample storage. Here, we explore the efficacy of a commercially available stool collection device with a stabilization reagent tailored to fecal metabolomics. Stool samples from six donors were either processed shortly post-collection or stored at room temperature for seven days in the tube, with and without the stabilization reagent. Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOFMS)-based untargeted metabolomics was utilized for analyzing extracted metabolites. Chemometric analysis was used to evaluate the performance of the device. We found that the device with the stabilization reagent minimized changes in the metabolite profile relative to unstabilized stool left at room temperature for one week. Full article

To investigate the axial compression behavior of composite columns with high-strength concrete-filled steel tube flanges and honeycombed steel web (STHHC) under load during freeze–thaw cycles, 48 full-scale composite column specimens were designed with different parameters: the restraint effect coefficient (ξ), concrete strength (f_cu), number of freeze–thaw cycles (n_d), slenderness ratio (λ), space–height ratio (s/h_w), and hole–height ratio (d/h_w). The finite element models of STHHC composite columns were simulated using ABAQUS finite element software (Version: 2021). The modeling method’s rationality was verified by comparing simulation results with experimental outcomes. Based on the finite element model, a parametric analysis of the composite columns under freeze–thaw cycles was conducted, analyzing their failure modes and load-bearing processes. The results indicate that the bearing capacity of the STHHC increased with increases in ξ and f_cu, and decreased with a rise in λ. In contrast, the influence of s/h_w and d/h_w on the ultimate bearing capacity of the composite columns was relatively minor. An equation for calculating the axial bearing capacity of the STHHC composite columns under freeze–thaw cycles was derived using statistical regression methods and considering the impact of different parameters on the axial compressive performance of the composite columns, laying the foundation for the promotion and application of this type of composite column in practical engineering projects. Full article

Steel pipes are commonly used to strengthen the concrete’s load-bearing capacity. However, they are prone to corrosion in salt erosion environments. In this study, the influence of Na₂MoO₄ and benzotriazole on concrete-filled steel tubes’ corrosion performance is investigated. The steel pipes’ mass loss rates (MRs), ultrasonic velocity, electrical resistance, and the AC impedance spectrum and Tafel curves of concrete-filled steel tubes were used to characterize the degree of corrosion in the steel pipes. Scanning electron microscopy–energy-dispersive spectrometry and X-ray diffraction were used for studying the composition of steel pipe rust. The research results revealed that the NaCl freeze–thaw cycles (F-C) and NaCl dry–wet alternation (D-A) actions had a reducing effect on the mass and ultrasonic velocity of the concrete-filled steel tubes. After 300 NaCl F-C and 30 NaCl D-A, the MRs were 0%~0.00470% and 0%~0.00666%. The corresponding ultrasonic velocities were 0%~21.1% and 0%~23.6%. When a rust inhibitor was added, the results were the opposite. The MRs decreased by 0%~80.3% and 0%~81.6% with the added Na₂MoO₄ and benzotriazole. Meanwhile, the corresponding ultrasonic velocities were 0%~8.1% and 0%~8.3%. The steel tubes were corroded after 300 NaCl F-C and 30 NaCl D-A. The addition of rust inhibitors improved the corrosion resistance of the concrete-filled steel tubes by increasing the electrical resistance before NaCl erosion. The corrosion area rate decreased by using the rust inhibitors. The corrosion resistance effect of benzotriazole was higher than that of Na₂MoO₄. The concrete-filled steel tube with an assembly unit comprising 5 kg/m³ of Na₂MoO₄ and 15 kg/m³ of benzotriazole had the best corrosion resistance under the erosion induced by NaCl F-C and D-A. Rust inhibitors reduced the content of iron-containing crystals and iron elements. The specimens with 5 kg/m³ Na₂MoO₄ and 15 kg/m³ benzotriazole had the lowest concentration of iron-containing crystals and iron elements. Full article