Search Results (42)

Goji berries (Lycium barbarum L.) are extremely rich in bioactive compounds, including phenolics, flavonoids, and vitamin C, which contribute to the strong antioxidant and immunomodulatory properties, positioning them as a promising candidate for nutraceutical applications. However, due to some limitations such as poor bioavailability and instability, encapsulation via spray drying with polymeric carriers provides a practical strategy to improve their stability, bioavailability, and applicability in the health sector. In this study, goji berry extract (GBE) was obtained via ultrasound-assisted extraction (UAE) and encapsulated using spray drying with four different polymers: alginate, pectin, Eudragit E100 and RS30D. GBE-loaded microparticles showed improved production yields (e.g., 40.3% for Alginate + GBE vs. 13.9% for Alginate alone) and varying particle sizes (1.9–4.4 µm). The antioxidant/antiradical activities were retained to different extents, depending on the carrier, with RS30D + GBE displaying the highest TPC (15.51 mg GAE (gallic acid equivalents)/g), FRAP (59.83 µmol FSE (ferrous sulphate equivalents)/g), and DPPH activities (3.50 mg TE (Trolox equivalents)/g). Biocompatibility was confirmed in HT29-MTX cell lines for all produced microparticles. These findings support the use of spray-dried polymeric carriers to enhance the functional performance and stability of goji berry bioactive compounds in future nutraceutical applications. Full article

An aqueous leaf extract of Egeria densa was used to green-synthesize iron (II) and iron (III) oxide nanoparticles from ferrous sulphate and ferric chloride, respectively. The successful green synthesis of the nanoparticles was confirmed through UV–visible spectroscopy, and the colour of the mixtures changed from light-yellow to green-black and reddish-brown for FeO–NPs and Fe₂O₃–NPs, respectively. The morphological characteristics of the nanoparticles were determined using an X-ray diffractometer (XRD), a Fourier transform infrared spectrophotometer (FTIR), a transmission electron microscope (TEM), and energy-dispersive X-ray spectroscopy (EDX). The UV–Vis spectrum of the FeO–NPs showed a sharp peak at 290 nm due to the surface plasmon resonance, while that of the Fe₂O₃–NPs showed a sharp peak at 300 nm. TEM analysis revealed that the FeO–NPs were oval to hexagonal in shape and were clustered together with an average size of 18.49 nm, while the Fe₂O₃-NPs were also oval to hexagonal in shape, but some were irregularly shaped, and they clustered together with an average size of 27.96 nm. EDX analysis showed the presence of elemental iron and oxygen in both types of nanoparticles, indicating that these nanoparticles were essentially present in oxide form. The XRD patterns of both the FeO–NPs and Fe₂O₃–NPs depicted that the nanoparticles produced were crystalline in nature and exhibited the rhombohedral crystal structure of hematite. The FT-IR spectra revealed that phenolic compounds were present on the surface of the nanoparticles and were responsible for reducing the iron salts into FeO–NPs and Fe₂O₃–NPs. Conclusively, this work demonstrated for the first time the ability of Elodea aqueous extract to synthesize iron-based nanoparticles from both iron (II) and iron (III) salts, highlighting its versatility as a green reducing and stabilizing agent. The dual-path synthesis approach provides new insights into the influence of the precursor oxidation state on nanoparticle formation, thereby expanding our understanding of plant-mediated nanoparticle production and offering a sustainable route for the fabrication of diverse iron oxide nanostructures. Furthermore, it provides a simple, cost-effective, and environmentally friendly method for the synthesis of the FeO–NPs and Fe₂O₃–NPs using Egeria densa. Full article

Background: Iron deficiency is a prevalent issue among elite athletes, particularly in endurance-based sports like football, where optimal iron status is crucial for aerobic capacity and performance. Despite the well-documented role of iron in oxygen transport and energy metabolism, the interplay between genetic polymorphisms, biochemical markers, and iron supplementation remains poorly understood. This study aimed to investigate the relationship between genetic polymorphisms and iron status in professional football players, assess the impact of iron supplementation on athletic performance, and develop a predictive model for iron supplementation based on genetic and biochemical profiles. Methods: A longitudinal study was conducted over three seasons (2021–2024) with 48 male professional football players. Participants underwent genotyping for polymorphisms in ACE (rs4646994), ACTN3 (rs1815739), AMPD1 (rs17602729), CKM (rs8111989), HFE (rs1799945), and MLCK (rs2700352, rs28497577). Biochemical markers (ferritin, haemoglobin, haematocrit, serum iron) and performance metrics (GPS-derived data) were monitored. Iron supplementation (105 mg/day ferrous sulphate) was administered to players with ferritin <30 ng/mL. A Total Genotype Score (TGS) was calculated to evaluate genetic predisposition. Results: Players with “optimal” genotypes (ACE DD, ACTN3 CC, AMPD1 CC, HFE GC) required less iron supplementation (TGS = 51.25 vs. 41.32 a.u.; p = 0.013) and exhibited better performance metrics. Iron supplementation significantly improved haemoglobin and haematocrit in deficient players (p < 0.05). The TGS predicted supplementation need (AUC = 0.711; p = 0.023), with a threshold of 46.42 a.u. (OR = 5.23, 95% CI: 1.336–14.362; p = 0.017 for non-supplemented players). Furthermore, performance data revealed that iron-supplemented players had significantly lower competition time (1128.40 vs. 1972.84 min; p = 0.003), total distance covered (128,129.42 vs. 218,556.64 m; p = 0.005), and high-speed running in the 18–21 km/h (7.58 vs. 10.36 m/min; p = 0.007) and 21–24 km/h (4.43 vs. 6.13 m/min; p = 0.010) speed zones. They also started fewer matches (11.50 vs. 21.59; p < 0.001). Conclusions: Genetic profile combined with biochemical monitoring effectively predicts iron supplementation needs in athletes. Personalized nutrition strategies, guided by TGS, can optimize iron status and enhance performance in elite football players. This approach bridges a critical gap in sports science, offering a framework for precision nutrition in athletics. Full article

Mineral waste represents the largest group of industrial waste, which constitutes a serious environmental and economic problem. This paper presents the phase and chemical susceptibility to leaching of coal mining wastes, steel and iron production slags, and non-ferrous metallurgical slags. These wastes, because of their properties, are used for the production of artificial aggregates. A detailed description of the slags’ properties (chemical and phase composition, technical characteristics, and releasability of constituents to the aquatic and soil environment) allows applications for such waste to be sought, which is beneficial for environmental and economic reasons. Coal mining wastes, slags from iron and steel production, and non-ferrous metallurgical slags are characterized by a variable chemical and phase composition, dominated by components with a relatively low degree of leaching, i.e., silicates, carbonates, sulphates, and intermetallic compounds. Therefore, these wastes are widely used for the production of artificial aggregates, perfectly meeting the requirements of the circular economy policy. Full article

The nutritional quality of plant-based meat analogues compared to traditional meat products has been questioned in recent commentary, particularly in relation to protein quality and micronutrient bioavailability. However, the attributes of specific products within this category are unclear. We therefore undertook a comprehensive assessment of the compositional and functional attributes of v2food^® (Sydney, Australia) plant-based mince, including an assessment of the effects of reformulation, including the addition of amino acids, ascorbic acid, and different forms of elemental iron. The protein digestibility and protein quality of v2food^® plant-based mince were comparable to beef mince in the standardized INFOGEST system, and favourable effects on microbiota composition and short-chain fatty acid (SCFA) production were demonstrated in an in vitro digestion system. The use of ferrous sulphate as an iron source improved in vitro intestinal iron absorption by ~50% in comparison to other forms of iron (p < 0.05), although levels were ~3-fold lower than beef mince, even in the presence of ascorbic acid. In conclusion, the current study identified some favourable nutritional attributes of plant-based v2food^® mince, specifically microbiota and SCFA changes, as well as other areas where further reformulation could be considered to further enhance the bioavailability of key nutrients. Further studies to assess the effect of plant-based meat analogues on health measures in vivo will be important to improve knowledge in this area. Full article

Disphyma crassifolium, commonly known as sea fingers, is a halophyte plant recently introduced in gourmet cuisine. The present study aims to extract the bioactive compounds of D. crassifolium using ultrasound-assisted extraction and employing green solvents (water and ethanol). The antioxidant/antiradical activities, scavenging capacity against reactive species, phenolic profile, and intestinal effects were evaluated. The highest total phenolic (53.13 mg of gallic acid equivalent (GAE)/g on dry weight (dw)) and flavonoid contents (18.98 mg of catechin equivalent (CE)/g dw) as well as antioxidant (149.69 µmol of ferrous sulphate equivalent (FSE)/g dw) and antiradical capacities (9.37 mg of ascorbic acid equivalent (AAE)/g dw) were achieved for the alcoholic extract. Moreover, the alcoholic extract exhibited an efficient uptake of HOCl (IC₅₀ = 1.97 µg/mL) and ROO^• (0.34 μmol of Trolox equivalent (TE)/mg dw). A total of 34 phenolic compounds were identified in the extracts, with flavonols (isorhamnetin-3-O-rutinoside, quercetin-3-O-galactoside, and myricetin), flavanols (catechin), and phenolic acids (gallic and ellagic acids) being the principal classes. The intestinal cell viability assays attested that the alcoholic extract presented the lowest IC₅₀ values (289.82 and 35.77 µg/mL for HT29-MTX and Caco-2), showing probable anticancer activity. These results emphasize the potential of D. crassifolium as a nutraceutical ingredient. Full article

(1) Background: Postpartum anemia is a common maternal complication and is recognized as a cause of impaired quality of life, reduced cognitive abilities, and fatigue. Efficient iron supplementation for the treatment of postpartum anemia is an essential component of high-quality maternal care. The optimal mode of iron supplementation has not been determined yet, whether oral or intravenous. The objective of this study was to compare postpartum anemia treatment with intravenous ferric carboxymaltose, intravenous ferric derisomaltose, and oral ferrous sulfate. (2) Methods: A single-center, open-label, randomized controlled trial. Women with hemoglobin < 100 g/L within 48 h postpartum were randomly allocated to receive intravenous ferric carboxymaltose, intravenous ferric derisomaltose, or oral ferrous sulfate. Intravenous iron was given in one or two doses, while ferrous sulfate was given as two 80 mg tablets once daily. The primary outcome was maternal fatigue measured by the Multidimensional Fatigue Inventory (MFI) six weeks postpartum. Hemoglobin, ferritin, and transferrin saturation levels were analyzed as secondary outcomes. A Kruskal–Wallis test was used for group comparison (p < 0.05 significant). (3) Results: Three hundred women were included. The MFI score at six weeks postpartum did not differ between groups (median 38 (inter-quartile range (IQR) 29–47) in the ferric carboxymaltose group, median 34 (IQR 26–42) in the ferric derisomaltose group, and median 36 (IQR 25–47) in the ferrous sulfate group; p = 0.26). Participants receiving oral iron had lower levels of hemoglobin (135 (131–139) vs. 134 (129–139) vs. 131 (125–137) g/L; p = 0.008), ferritin (273 (198–377) vs. 187 (155–246) vs. 24 (17–37) µg/L; p < 0.001) and transferrin saturation (34 (28–38) vs. 30 (23–37) vs. 24 (17–37) %; p < 0.001) than those receiving ferric carboxymaltose or ferric derisomaltose. (4) Conclusions: Intravenous ferric carboxymaltose, intravenous ferric derisomaltose, and oral ferrous sulfate had similar impacts on maternal fatigue at six weeks postpartum despite improved laboratory parameters in the intravenous groups. Full article

The textile industry stands as a prominent contributor to global environmental pollution, primarily attributable to its extensive reliance on synthetic dyes, hazardous components, and solvents throughout the textile dyeing and treatment processes. Consequently, the pursuit of sustainable textile solutions becomes imperative, aimed at replacing these environmentally unfriendly constituents with biobased and bioactive pigments, antibacterial agents, and, notably, natural solvents. Achieving this goal is a formidable yet indispensable challenge. In this study, the dyeing ability of the crude gel prodigiosin, produced by non-pathogenic bacteria Serratia plymuthica, was investigated on various multifiber fabrics at different conditions (temperature and pH) and by using salts and alternative mordants (the conventional Ferrous Sulphate (FeSO₄) and a new bio-mordant, L-Cysteine (L-Cys)). Additionally, a novel gel-based Choline chloride (ChCl)/Lactic acid (LA) (1:2) deep eutectic solvent (DES) dyeing medium was studied to replace the organic solvents. Nylon fabrics dyed with 3.0% over the weight of the fiber (owf) L-Cys at pH = 8.3 had improved color fastness to washing, while the gel-based ChCl/LA (1:2) DES dyebath provided a better color fastness to light. Moreover, nylon fabrics under these conditions exhibited remarkable antimicrobial activity against Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa). In conclusion, the utilization of the crude gel-based prodigiosin pigment demonstrates a distinct advantage in dyeing textile materials, aligning with the growing consumer demand for more eco-friendly and sustainable products. Additionally, the application of the natural reducing agent L-Cys, previously untested as a bio-mordant, in conjunction with the use of gel-based DES as a dyeing medium, has showcased improved colorimetric and antibacterial properties when applied to nylon that is dyed with the crude gel prodigiosin pigment. Full article

This work proposes an environmentally safe and economically feasible method of waste copper-nickel production slag utilization (Kola Mining-Metallurgical Company, Nornickel, Kola Peninsula, Russia). This process involves the decomposition of slag by diluted solutions of sulfuric acid (7–10 wt.% H₂SO₄) with a transfer in a solution of more than 70% silicon, 77% iron and 78% magnesium, and a concentration of non-ferrous metals in the residue (~70%). Copper ions were used in the leaching stage to prevent the release of hydrogen sulfide into the working atmosphere. Dehydration of the solution, followed by washing of water-soluble sulphates from silica, was carried out to separate silica from the leaching solution. The dehydration temperature effect on the silica structural characteristics was determined. The possibility of recovering non-ferrous metals from solutions after silica extraction by precipitation, in the form of copper cementite, and the sum of nickel and cobalt sulfides, was evaluated. Pigment-grade iron dioxide, magnesium sulphate and aluminium hydroxide were obtained by dehydration of the solution after extraction of base metals, calcination and other operations. Sulfuric acid leaching resulted in the disclosure of sulfide grains encapsulated in ferrosilicate, which is a favorable factor for flotation. The depleted residue can be successfully used in the construction industry. Full article

It is a well-known fact that water bodies are crucial for human life, ecosystems and biodiversity. Therefore, they are subject to regulatory monitoring in terms of water quality. However, land-use intensification, such as open-cast mining activities, can have a direct impact on water quality. Unfortunately, in situ measurements of water quality parameters are spatially limited, costly and time-consuming, which is why we proposed a combination of hyperspectral data, in situ data and simple regression models in this study to estimate and thus monitor various water quality parameters. We focused on the variables of total iron, ferrous iron, ferric iron, sulphate and chlorophyll-a. Unlike other studies, we used a combination of airborne hyperspectral and RGB data to ensure a very high spatial resolution of the data. To investigate the potential of our approach, we conducted simultaneous in situ measurements and airborne hyperspectral/RGB aircraft campaigns at different sites of the Spree River in Germany to monitor the impact of pyrite weathering on water bodies after open-cast mining activities. Appropriate regression models were developed to estimate the five variables mentioned above. The model with the best performance for each variable gave a coefficient of determination R² of 64% to 79%. This clearly shows the potential of airborne hyperspectral/RGB data for water quality monitoring. In further investigations, we focused on the use of machine learning techniques, as well as transferability to other water bodies. The approach presented here has great potential for the development of a monitoring method for the continuous monitoring of still waters and large watercourses, especially given the freely available space-based hyperspectral missions via EnMAP. Full article

Turfgrasses are considered an important part of the landscape and ecological system of golf courses, sports fields, parks, and home lawns. Turfgrass species are affected by many abiotic stresses (e.g., drought, salinity, cold, heat, waterlogging, and heavy metals) and biotic stresses (mainly diseases and pests). In the current study, seashore paspalum (Paspalum vaginatum Sw.) and Tifway bermudagrass (Cynodon transvaalensis Burtt Davy × C. Dactylon) were selected because they are popular turfgrasses frequently used for outdoor lawns and sport fields. The effect of the combined stress from both soil salinity and cold on these warm season grasses was investigated. Some selected organic and inorganic amendments (i.e., humic acid, ferrous sulphate, and silicon) were applied as foliar sprays five times during the winter season from late October to March. This was repeated over two years in field trials involving salt-affected soils. The physiological and chemical parameters of the plants, including plant height; fresh and dry weight per plot; total chlorophyll content; and nitrogen, phosphorus, iron, and potassium content, were measured. The results showed that all the studied amendments improved the growth of seashore paspalum and Tifway bermudagrass during this period compared to the control, with a greater improvement observed when using ferrous sulphate and humic acid compared to silicon. For seashore paspalum, the highest chlorophyll content in April was recorded after the application of ferrous sulphate at a level of 1000 ppm. The current research indicates that when grown on salt-affected soils, these amendments can be used in warm-season grasses to maintain turf quality during cold periods of the year. Further research is needed to examine any negative long-term effects of these amendments and to explain their mechanisms. Full article

Many sandy soil foundations need to be solidified during traffic construction in Guangxi, China. Because it has a similar chemical composition as cement, municipal solid waste incineration fly ash (MSWIFA) can strengthen sandy soil. However, the chloride ions and heavy metals in MSWIFA may have a negative influence on the solidification of sandy soil. Thus, FA resource use faces great challenges. This study evaluates the feasibility of using MSWIFA to solidify sandy soil. The acetic acid buffer solution method was used in the leaching test to simulate the weak acid groundwater environment in the Guangxi karst landform. The effects of the treatment methods (washing with ferrous sulphate solution, pre-treatment of organics via chelation, and adding sugarcane ash) on the strength and environmental characteristics of fly ash cement-stabilised soil (FACS) are discussed in detail. The results indicate that the FACS unconfined compressive strength (UCS) decreased by 24.82–46.64% when 5% cement was replaced with FA. Sugarcane ash effectively improved the strength of FACS by more than 10%. The leaching concentrations of Zn and Cu in the FACS meet the concentration limit set by GB 16889-2008. The leaching concentrations of Cr and Pb after washing with 6% ferrous sulphate solution were reduced by more than 30%. Meanwhile, the FACS strength developed faster. Organic chelating agents solidified most heavy metals. Full article

A single-center, crossover, randomized, double-blind, and controlled clinical study was conducted to assess the tolerability profile, especially with regard to gastrointestinal complaints, of oral supplementation with AB-Fortis^®, a microencapsulated ferric saccharate (MFS), as compared with conventional ferrous sulphate (FS) in healthy premenopausal women. A dose of 60 mg/day of elemental iron was used. The test products were administered for 14 consecutive days with a washout period of two menstrual episodes and a minimum of one month between the two intervention periods. The subjects completed simple-to-answer questionnaires daily for 14 days during both the intervention and the washout periods, capturing the symptoms associated with oral iron supplementation and overall health aspects. Following product consumption, the incidences of symptoms, numbers of complaints/symptoms, overall intensity, and total days with symptoms were found to be significantly higher for FS consumption as compared to MFS. The better tolerability profile of MFS over FS was further substantiated when both products were compared to a real-life setting (i.e., the washout period). Overall, the administration of both study products was safe with no serious or significant adverse events reported. In summary, the current study shows the better tolerability of the MFS preparation when compared to that of the FS, presenting MFS as a well-tolerated and safe option for improving iron nutrition. Full article

(1) Background: Iron deficiency anemia is a significant nutritional problem all over the world. Salt formulations supplemented with encapsulated iron and iodine (double-fortified) were tested for their efficacy in managing iron deficiency anemia. In this study, we have checked the effect of these double-fortified salt formulations (iron and iodine) on hemoglobin (Hb) levels in anemic Wistar male rats. (2) Methods: The study was divided into two phases, viz., the development of anemia in the first phase and then the random division of anemic rats into five groups (Groups A to E). These rats were fed with three different salt formulations (Groups A to C); Group D was continued on a low iron diet, and Group E was on a normal pellet diet over a period of 84 days. The level of Hb was tested in each group. (3) Results: The rats in Groups A, B, C, and E recovered from anemia significantly, with higher Hb levels. On day 84, however, the Hb level in Group D continued to decrease. The bodyweight of the rats was not affected in any way. In all of the groups, histopathology examinations in various organs revealed no significant changes. (4) Conclusions: All of the three different salt formulations showed significant recovery in the anemic rats as compared to the rats fed with a normal pelleted diet. Full article

Anthocyanin-rich black cane (aBC) is a grass rich in lignin and carbohydrates, with an abundance of anthocyanins. Silages of aBC produced with molasses (MS) and/or ferrous sulphate (FS) mixtures may have beneficial effects on silage quality and animal performance in ruminants. However, the addition of MS and FS to ensiled grass is relatively unexplored. Therefore, this study systematically evaluated the effect of their administration at different doses to select an effective treatment to modulate the ensiling characteristics of aBC. In the first trial, fresh or pre-ensiled materials (PBC) were compared with ensiled PBC treated with: 0% MS 0% FS, 4% MS, 8% MS, 0.015% FS, 0.030% FS, 4% MS + 0.015% FS, 4% MS + 0.030% FS, 8% MS + 0.015% FS, and 8% MS + 0.030% FS on a fresh matter basis. The quality of ensiling characteristics was determined in laboratory-scale silos after 42 d of preservation. Based on these results, the second trial was further conducted in rumen cultures to ensure that the selected treatment would not impair rumen fermentation. For this, ruminal biogases, rumen fermentation profiles, and microbial communities were evaluated. Ensiled PBC with the incremental addition of MS and FS resulted in the observations for anthocyanin contents and the ensiling characteristics of the aBC silages. The combination of MS (4%) and FS (0.030%) incorporated into silages had the highest silage production effect among the experimental treatments. This combination demonstrated the sustainable mitigation of the ruminal biogases of methane and carbon dioxide without impairment of total gas production. Concurrently, this combination improved total volatile fatty acid concentrations, modulated cellulolytic bacteria, and suppressed methanogenic bacteria in rumen fluids. The results presented here indicated that addition of a mixture of 4% MS and 0.030% FS to aBC resulted in an optimal balance of ensiling characteristics and is suitable for use in ruminants. Full article