Search Results (4,985)

The bacterial phylum Verrucomicrobiota accommodates free-living and symbiotic microorganisms, which inhabit a wide range of environments and specialize in polysaccharide degradation. Due to difficulties in cultivation, much of the currently available knowledge about these bacteria originated from cultivation-independent studies. A phylogenetic clade defined by the free-living bacterium from oilsands tailings pond, Oleiharenicola alkalitolerans, and the symbiont of the tunicate Lissoclinum sp., Candidatus Didemniditutus mandelae, is a poorly studied verrucomicrobial group. This clade includes two dozen methagenome-assembled genomes (MAGs) retrieved from aquatic and soil habitats all over the world. A new member of this clade, strain Vm1, was isolated from a methane-fed laboratory bioreactor with a Methylococcus-dominated methane-oxidizing consortium and characterized in this study. Strain Vm1 was represented by ultra-small, motile cocci with a mean diameter of 0.4 µm that grew in oxic and micro-oxic conditions at temperatures between 20 and 42 °C. Stable development of strain Vm1 in a co-culture with Methylococcus was due to the ability to utilize organic acids excreted by the methanotroph and its exopolysaccharides. The finished genome of strain Vm1 was 4.8 Mb in size and contained about 4200 predicted protein-coding sequences, including a wide repertoire of CAZyme-encoding genes. Among these CAZymes, two proteins presumably responsible for xylan and arabinan degradation, were encoded in several MAGs of Vm1-related free-living verrucomicrobia, thus offering an insight into the reasons behind wide distribution of these bacteria in the environment. Apparently, many representatives of the Oleiharenicola–Candidatus Didemniditutus clade may occur in nature in trophic associations with methanotrophic bacteria, thus participating in the cycling of methane-derived carbon. Full article

Understanding human perceptual strategies in high-stakes environments, such as crime scene investigations, is essential for developing cognitive models that reflect expert decision-making. This study presents an immersive experimental framework that utilizes virtual reality (VR) and eye-tracking technologies to capture and analyze visual attention during simulated forensic tasks. A${360}^{\circ }$ panoramic crime scene, constructed using the Nikon KeyMission 360 camera, was integrated into a VR system with HTC Vive and Tobii Pro eye-tracking components. A total of 46 undergraduate students aged 19 to 24–23, from the National University of Singapore in Singapore and 23 from the Central Police University in Taiwan—participated in the study, generating over 2.6 million gaze samples (IRB No. 23-095-B). The collected eye-tracking data were analyzed using statistical summarization, temporal alignment techniques (Earth Mover’s Distance and Needleman-Wunsch algorithms), and machine learning models, including K-means clustering, random forest regression, and support vector machines (SVMs). Clustering achieved a classification accuracy of 78.26%, revealing distinct visual behavior patterns across participant groups. Proficiency prediction models reached optimal performance with a random forest regression ($R^2$ = 0.7034), highlighting scan-path variability and fixation regularity as key predictive features. These findings demonstrate that eye-tracking metrics—particularly sequence-alignment-based features—can effectively capture differences linked to both experiential training and cultural context. Beyond its immediate forensic relevance, the study contributes a structured methodology for encoding visual attention strategies into analyzable formats, offering valuable insights for cognitive modeling, training systems, and human-centered design in future perceptual intelligence applications. Furthermore, our work advances the development of autonomous vehicles by modeling how humans visually interpret complex and potentially hazardous environments. By examining expert and novice gaze patterns during simulated forensic investigations, we provide insights that can inform the design of autonomous systems required to make rapid, safety-critical decisions in similarly unstructured settings. The extraction of human-like visual attention strategies not only enhances scene understanding, anomaly detection, and risk assessment in autonomous driving scenarios, but also supports accelerated learning of response patterns for rare, dangerous, or otherwise exceptional conditions—enabling autonomous driving systems to better anticipate and manage unexpected real-world challenges. Full article

This paper explores how school culture and key organizational culture dimensions, power distance, humane orientation, performance orientation, and in-group collectivism, affect different types of conflict (task, relationship, and process) in primary schools in Serbia. It also examines how gender and teachers’ organizational commitment moderate these relationships. Data were obtained by surveying 380 respondents, all of whom were primary school teachers in Serbia. The participants were between 23 and 65 years old. Of the total sample, 19.47% were male, and 80.53% were female. The mean values ranged from 1.8046 to 4.9847, with standard deviations between 0.7699 and 1.4785. The research was conducted using a simple random sampling technique. Teachers were given questionnaires through Google Forms, which they completed online. Printed versions were also distributed and later entered into the database. The study was guided by two research questions and two hypotheses. Data analysis was performed using SPSS (Statistical Package for the Social Sciences). The findings reveal that the school culture dimension of teacher professionalism and goal setting (r = −0.297 **; β = −0.496) and the organizational culture dimension of humane orientation (r = −0.303 **; β = −0.198) have the most substantial negative effects on conflict, indicating their beneficial role in reducing it. In contrast, power distance shows a strong positive relationship with conflict, particularly with relationship conflict (r = 0.230 **; β = 0.201). Additionally, excessive emphasis on teacher collaboration and performance orientation appears to increase relationship conflict (β = 0.226; β = 0.261, respectively). Gender differences emerged: cultural dimensions were more effective in reducing conflict among women than men. Among male teachers, power distance had a stronger influence, significantly increasing task conflict (r = 0.303 **). The school culture and organizational culture dimensions significantly reduce the conflict dimensions in the case of high teacher commitment. The dimension power distance has a statistically significant and positive effect on conflicts when organizational commitment is high (r = 0.247 **). Therefore, school culture and organizational culture dimensions achieve stronger effects in committed women, while power distance achieves stronger effects in committed men. Full article

Background/Objectives: Although people with schizophrenia appear to experience emotions like healthy individuals, previous studies suggest that their ability to engage in empathic emotional responses might be impaired. As per our knowledge, no studies in the Arab world have investigated empathy in patients with schizophrenia, which is likely due to the lack of valid and reliable measures to assess the empathy construct among Arabic-speaking people. The aim of this research is to validate the Arabic version of the Pictorial Empathy Test (PET) in patients with schizophrenia from Lebanon. Methods: A two-month cross-sectional study was carried out at the Psychiatric Hospital of the Cross during January and February of 2024. The average age of the 113 participants in this study was 57.52 ± 10.35 years and 63.5% of them were men. Data were collected through in-person interviews. A confirmatory factor analysis (CFA) was conducted using SPSS AMOS version 29. Parameter estimation utilized the maximum likelihood approach. In order to examine sex invariance in PET scores, a multi-group CFA was conducted. Measurement invariance was assessed across configural, metric, and scalar levels. Evidence of invariance was determined based on the criteria: ΔCFI ≤ 0.010, ΔRMSEA ≤ 0.015, or ΔSRMR ≤ 0.010. Results: CFA revealed that the Arabic PET exhibited a unidimensional factor structure. The PET demonstrated solid internal consistency (ω = 0.93, α = 0.93). Measurement invariance testing confirmed that the scale performed equally well across sexes. A linear regression analysis found that female sex and higher levels of alexithymia were significantly correlated with lower levels of affective empathy. Conclusions: The findings indicate that the Arabic version of the PET is a reliable and valid tool for measuring affective empathy in Arabic-speaking patients with schizophrenia. The culturally adapted and validated Arabic PET would help detect affective empathy deficits, design and implement context-tailored interventions, and encourage future research in this area in the Arab region. Future research should aim to validate the PET against behavioral tasks like the Empathic Accuracy Task to improve its ecological validity. Full article

Objectives: This study aimed to investigate the effects of the quorum-quenching enzyme N-acyl-homoserine lactone (AHL)-lactonase Est816 on biofilm formation in subgingival plaque microbiota from participants with advanced periodontitis. Methods: Subgingival plaque samples were collected from 30 adults with untreated Stage III or higher periodontitis and cultured anaerobically. Est816 was applied in vitro, with phosphate-buffered saline (PBS) serving as the control. Biofilm composition was analyzed via 16S rRNA sequencing, and alpha diversity metrics were assessed. Differential taxa abundance was assessed with the multivariate statistical software MaAsLin3. Biofilm morphology, biomass, and thickness were evaluated using scanning electron microscopy (SEM), crystal violet staining, and confocal laser scanning microscopy (CLSM). Results: Est816 significantly reduced microbial richness (Chao1 Index, p = 0.031), biofilm biomass (64% reduction, p < 0.001), and thickness (76% reduction, p < 0.001) compared to controls. SEM revealed fragmented biofilm architecture in Est816-treated samples. Conclusions: AHL-lactonase Est816 inhibited polymicrobial subgingival-plaque-derived biofilm formation while reducing species richness, phylogenetic diversity, and community evenness. These findings demonstrate Est816’s potential as an adjunctive therapy for disrupting pathogenic biofilms in periodontitis. Full article

This study aims to analyze the effects of responsible consumption behavior on impulsive buying, and the mediating role of brand literacy in this relationship. Data collected from 524 Turkish participants using an online survey were analyzed using structural equation modeling (SEM). Responsible consumerism exhibited a negative direct effect on impulsive buying and a positive effect on brand literacy. Brand literacy, in turn, was negatively associated with impulsive buying and partially mediated the responsible consumerism–impulsive buying link. Over-consumption and impulsive buying have received considerable scholarly attention. Yet, only a small number of studies have tested whether value-driven orientations, such as responsible consumerism, can curb these impulses. Even fewer still probe the mediating cognitive role of consumer knowledge (e.g., brand literacy). Furthermore, most existing evidence comes from Western high-income settings. This study addresses that gap by empirically testing the responsible consumerism, brand literacy, and impulsive buying pathway in Türkiye, an emerging and rapidly digitalizing economy. Considering its cross-sectional nature and cultural limitations, this study recommends conducting future longitudinal studies and research in various cultural contexts. Full article

In Thailand, companies are facing increasing pressure from investors, consumers, customers, and regulators to integrate sustainability into business policies and practices. Achieving corporate sustainable development requires incorporating environmental attitudes and work environments into employee behaviors. This study examines how perceived sustainability policies (PSP) influence pro-environmental behaviors (PEB) in the workplace. A total of 589 respondents from four Thai companies in diverse sectors—rubber, consumer products, B2B industrials, and garments—participated in the study. Grounded in the Focus Theory of Normative Conduct, the research extends individual-level psychological frameworks by incorporating the mediating roles of organizational-level descriptive norms—green shared vision (GSV) and green work climate (GWC)—and the moderating role of individual green value (IGV). Structural equation modeling was conducted using AMOS. The findings supported both a direct effect of PSP on PEB and a sequential mediation pathway through GSV and GWC, while the individual mediation roles of GSV and GWC were not significant. These results reflected strong institutional and in-group collectivist culture of Thailand. Moreover, IGV was found to have a significantly negative moderating effect, suggesting that employees with high IGV may rely less on formal perceived sustainability policies in shaping their pro-environmental behaviors. Full article

As hybrid organizations that combine market-related logic with social logic, social enterprises (SEs) play a crucial role in addressing the challenges faced by people in employment while creating economic benefits through innovative solutions to drive economic and social rural revitalization. Focusing on social enterprises, this study employs environmental analysis and theoretical reasoning to examine the logic and approaches through which social enterprises participate in rural revitalization in China’s northwest regions from the perspective of “new-quality productive forces.” Research findings indicate that in the face of an external environment characterized by weakened investment and consumption and the decline of the demographic dividend, and an internal environment marked by lagging socio-economic development and ecological fragility, SEs should integrate devotion and profit logic based on a mixed action logic of multi-value co-creation. Through approaches such as cultivating new quality talent, constructing a new quality industrial system, shaping a new quality rural environment, promoting synergistic innovation between science and technology and culture, and actively participating in the revitalization of rural areas in China’s northwest region, SEs can play an active role in China’s rural revitalization efforts. Full article

This research examines the connection between leadership and sustainable development, emphasizing how community involvement moderates it. It seeks to analyze the impact of leadership styles on sustainable development practices in three distinct cultural and economic contexts: Pakistan, India, and Taiwan. The study participants include managers, entrepreneurs, and consultants. A quantitative research methodology, which included questionnaires and statistical analysis, was used to collect data from business professionals in these three nations. This study considers the viewpoints of both general business leaders and the insights provided by small and medium-sized enterprises, which play a crucial role in driving economic growth in these areas. For example, 99% of Pakistan’s enterprises are small and medium-sized enterprises. However, due to financial constraints, small and medium-sized enterprises face obstacles such as limited innovative capacity. Small and medium-sized enterprises are vital to economic growth in Taiwan and India as well. Small and medium-sized enterprises generate many jobs and contribute substantially to GDP. Effective leadership is critical for promoting sustainability goals, as the findings show that leadership benefits sustainable development projects (H1). Sustainable development outcomes are greatly improved when the community is actively involved (H2), further demonstrating the significance of community involvement as a key component of effective sustainability measures. Contrary to expectations (H3), community involvement did not moderate the relationship between leadership and sustainable development as hypothesized; rather, it appeared as an independent factor that positively influenced sustainability outcomes. The findings highlight the broad relevance of effective leadership practices in various socioeconomic contexts. This study offers valuable insights for policymakers seeking to promote sustainable growth by emphasizing the importance of effective leadership practices and active community engagement within small and medium-sized enterprises. Full article

Since the turn of the 21st century, urban studies and planning research has examined the strategic role of artists, arts organizations, and cultural activity as local and regional economic development catalysts. This article shifts the spotlight from the “creative class” and “creative industries” as drivers of a “creative city” to study the role of art, culture, and creative practices in community-led, place-based efforts to stabilize neighborhoods and advance more hopeful, healthy, and equitable urban futures. It explores Boston’s Chinatown, where community-based art activism has a long history of addressing critical issues such as reclaiming land taken by interstate highway and urban renewal projects, as well as combating gentrification and displacement through site activation. The case study focuses on Residence Lab, a community-based arts residency program initiated by the Pao Arts Center and the Asian Community Development Corporation that brought together multimedia artists with residents to collectively preserve Boston’s Chinatown through creative and artistic activation of underutilized sites in the neighborhood from 2019 to 2022. We examine a selection of ResLab projects, which give form and meaning to the struggles and aspirations of being at home in Chinatown and embody the art activism of partner organizations and program participants, along with the ResLab’s impacts on participating residents and artists. The concluding discussion considers ResLab’s contributions and implications for the shifting ways in which urban, political, and artistic cultures have intersected and impacted one another in Chinatown along with the relationship between collective action and the preservation and transformation of culture in the urban frame. Full article

Background: The Mediterranean diet is widely recognised for its health benefits and remains a key reference point in shaping dietary guidelines across populations. Despite its growing international relevance, there is a lack of validated tools assessing Mediterranean diet adherence among children and adolescents in Central and Eastern Europe. Methods: The present study aimed to adapt and validate the KIDMED 2.0 questionnaire for use in Polish children and adolescents aged 10 to 18 years (KIDMED 2.0 PL). The adaptation process involved forward–backward translation, expert consultations, and pilot testing to ensure linguistic and cultural relevance. A total of 102 participants completed the questionnaire twice over a two-week interval, and anthropometric data were collected. Results: The KIDMED 2.0 PL demonstrated high test–retest reliability (Spearman’s ρ = 0.876; p < 0.001) and strong criterion validity, with a significant negative correlation between KIDMED scores and BMI centile (ρ = −0.854; p < 0.001). Children with normal weight showed the highest adherence to the Mediterranean diet, while scores were significantly lower in overweight and obese participants. Item-level analysis indicated that fruit and vegetable consumption was relatively frequent, whereas intake of legumes, whole grains, and extra virgin olive oil remained low. Conclusions: The KIDMED 2.0 PL is a valid and reliable tool for evaluating diet quality and Mediterranean dietary adherence in the Polish pediatric population. Full article

Background/Objective: Pediatric feeding problems are becoming more widespread. They represent a synthesis of social, behavioral, and biological issues. Inevitably, the multifaceted nature of these problems has to be evaluated through one multidimensional tool. The Pediatric Assessment Scale for Severe Feeding Problems is designed to assess these complex issues and represents the first attempt to associate these factors into a single multidimensional measure. The aim of this study is to translate and culturally adapt the Pediatric Assessment Scale for Severe Feeding Problems into Greek and to assess its psychometric properties for use among a Greek Cypriot pediatric sample. Methods: This study involved 142 parents of children with symptoms of feeding problems. It included parents of children who were oral-fed (n = 65), partially oral-fed with supplementation (n = 62), and tube-fed (n = 15). The sample of parents was recruited from mainstream and special education schools in Cyprus and were asked to complete a Greek-translated version of the PASS-FP. Results: The PASS-FP-Gr demonstrated excellent psychometric properties. Internal consistency was good, and test–retest reliability showed a perfect Spearman’s rank correlation with high significance. The tool exhibited strong discriminatory ability, with statistically significant differences in median scores across the three feeding groups. Conclusions: The PASS-FP appears sensitive to the Greek Cypriot population and presents satisfactory psychometric features. It demonstrated excellent discriminatory ability, as evidenced by the participants’ consistent response patterns. Full article

Sign language avatar systems have emerged as a promising solution to bridge communication gaps where human sign language interpreters are unavailable. However, the design of these avatars often fails to account for the diversity in how users acquire and perceive sign language. This study presents a rapid review of 17 empirical studies (2020–2025) to synthesize how linguistic and cognitive variability affects sign language perception and how these findings can guide avatar development. We extracted and synthesized key constructs, participant profiles, and capture techniques relevant to avatar fidelity. This review finds that delayed exposure to sign language is consistently linked to persistent challenges in syntactic processing, classifier use, and avatar comprehension. In contrast, early-exposed signers demonstrate more robust parsing and greater tolerance of perceptual irregularities. Key perceptual features, such as smooth transitions between signs, expressive facial cues for grammatical clarity, and consistent spatial placement of referents, emerge as critical for intelligibility, particularly for late learners. These findings highlight the importance of participatory design and user-centered validation in advancing accessible, culturally responsive human–computer interaction through next-generation avatar systems. Full article

Preterm birth is a leading cause of perinatal morbidity and mortality and is particularly prevalent among socially disadvantaged female populations. This quantitative, cross-sectional study aimed to explore the prevalence of preterm birth in three segregated Roma communities in Hungary and to identify health behavior and care factors associated with its occurrence. In our study, preterm birth was defined as delivery before 37 completed weeks of gestation (i.e., <259 days). Data were collected from 231 Roma women living in three municipalities of Szabolcs-Szatmár-Bereg County, one of Hungary’s most disadvantaged regions, using a structured interview questionnaire. The participants were women aged 18–65 years. Of these, 209 had been pregnant at least once in their lifetime. The questionnaire covered socio-demographic characteristics (age, level of education, employment status, housing conditions, marital status), health behaviors (smoking, alcohol consumption, drug use, vitamin supplementation, other substance use), antenatal care attendance, and birth outcomes (preterm birth, gestational age, low birth weight, newborn status). Statistical analyses included descriptive statistics, chi-square tests, and binary logistic regression with significance set at p < 0.05. Preterm birth was significantly more common among women who smoked, consumed alcohol or drugs during pregnancy, or had vaginal infections. Drug use showed the strongest association with a 22-fold increase in risk, followed by alcohol (nearly fivefold), smoking (over threefold), and infections (threefold). Although non-attendance at antenatal care was associated with increased risk, this relationship was not statistically significant. In the multivariate logistic regression model, alcohol consumption (OR = 1.744, p < 0.01), smoking (OR = 2.495, p < 0.01), drug use (OR = 25.500, p < 0.001), and vaginal infections (OR = 4.014, p < 0.01) during pregnancy were independently associated with an increased risk of preterm birth, whereas folic acid supplementation (OR = 0.448, p < 0.05) showed a significant protective effect. These findings highlight that preterm birth is intricately linked to socioeconomic disadvantage and adverse health behaviors. Culture-specific, community-based prevention strategies are essential to reduce perinatal risks in marginalized populations. Full article

China’s rapid urbanization has presented challenges for sustainably revitalizing the historic and cultural heritage within its urban villages. Often, these efforts overlook the crucial roles of community ties and cultural values. This study focuses on 15 representative urban villages in Guangzhou (2019–2024). It tests the core idea that the physical layout of these spaces reflects underlying community structures and cultural values shaped by specific policies. Integrating this understanding into landscape planning can significantly improve revitalization outcomes. We used a mixed-methods approach: (1) Extended fieldwork to understand community networks and cultural practices; (2) Spatial analysis to measure how building density relates to land uses; (3) Sentiment analysis to reveal how people perceive cultural symbols; (4) A coordination model to link population influx with landscape suitability. Key findings reveal different patterns: Villages with strong clan networks maintained high cultural integrity and public acceptance through bodies like ancestral hall councils. Economically driven villages showed a split—open for business but culturally closed, with very low tenant participation. Successful revitalization requires balancing three elements: protecting physical landmarks in their original locations; modernizing cultural events; and reconstructing community narratives. Practically, we propose a planning framework with four approaches tailored to different village types. For instance, decaying villages should prioritize repairing key landmarks that hold community memory. Theoretically, we build a model linking social and spatial change, extending the cultural value concepts of Amos Rapoport to the context of fast-growing cities. This provides a new methodological perspective for managing urban–rural heritage in East Asia. Full article