Search Results (16)

Curvature-based damage detection has been previously applied to identify damage in concrete structures, but little attention has been given to the capacity of this method to identify distributed damage in multiple damage zones. This study aims to apply for the first time an enhanced existing method based on modal curvature analysis combined with wavelet transform curvature (WTC) to identify zones and highlight the damage zones of a beam made of ultra-high-performance fiber-reinforced concrete (UHPFRC), a construction material that is emerging worldwide for its outstanding performance and durability. First, three beams with a 2 m span of UHPFRC material were cast, and damaged zones were created by sawing. A reference beam without cracks was also cast. The free vibration responses were measured by 12 accelerometers and calculated by operational modal analysis. Moreover, for the sake of comparison, a finite element model (FEM) was also applied to two identical beams to generate numerical acceleration without noise. Second, the modal curvature was calculated for different modes for both experimental and FEM-simulated acceleration after applying cubic spline interpolation. Finally, two damage identification methods were considered: (i) the damage index (DI), based on averaging the quadratic difference of the local curvature with respect to the reference beam, and (ii) the WTC method, applied to the quadratic difference of the local curvature with respect the reference beam. The results indicate that the developed coupled modal curvature WTC method can better identify the damaged zones of UHPFRC beams. Full article

We proposed an active differential intensity (DI) fiber coupled plasmon waveguide resonance (CPWR) sensor based on the mode competition effect in the C-band, aiming to enhance the sensitivity. The sensing head is a fiber probe with a sensing layer of ITO/Au/ITO/TiO₂ film, enabling the excitation of CPWR in the C-band. The narrow CPWR spectrum allows DI interrogation by tracking the intensity of light at two wavelengths. The fiber probe is inserted into a dual-wavelength fiber laser to adjust the intra-cavity loss at the two lasing wavelengths. By using the differential modulation of the reflectivity from the refractive index variations, the mode competition effect is triggered. The powers at two lasing wavelengths change oppositely, enlarging the power difference and then resulting in enhanced sensitivity. The average sensitivity is up to 5702 dB/RIUs, which is 10 times higher than that of conventional DI interrogation. The sensitivity enhancement mechanism based on the mode competition effect provides a new technical approach for enhancing the sensitivity of DI SPR sensors. Full article

As the surface acoustic wave (SAW) filters incline to ultrafine, the failures resulting from the wafer molding process have become increasingly prominent. A methodology for coupling the mechanisms of die fly and die shift for the SAW filter miniatured with 737 $\mathsf{\mu }\mathrm{m}$ × 517 $\mathsf{\mu }\mathrm{m}$ × 200 $\mathsf{\mu }\mathrm{m}$ is developed for the trade-off between reliability and yields. In terms of die fly and die shift, the former occurs before the epoxy molding compound (EMC) is cured in the temperature rise period, while the latter occurs in the cooling stage after being cured. The die fly is induced by the fluid flow force in the high-temperature stage of heat compression, which is fatal for the scrap. Followed by the cooling stage, the CTE (coefficient of thermal expansion) misalignment between the die and epoxy molding compound (EMC) seriously affects the die shift and the following lithography process yields. The debonding critical energy in the mixed mode is employed to avert the die fly. Then, the die fly can be shunned by fine-tuning the die thickness, die layout, and EMC layout. A methodology to measure die shift was conducted, by which a total of 47,568 dies were embedded using compression molding. The mechanical error of the mounter and the die shift law are comprehensively leveraged, indicating that the die shift can be controlled within 50 $\mathsf{\mu }\mathrm{m}$ for 8-inch wafer-level packaging. Full article

Differences between seven authentic samples of Citrus sinensis var. Valencia peel (albedo and flavedo) and juices from Spain and Uruguay, in addition to a concentrate obtained from Brazil, were investigated by untargeted metabolic profiling. Sixty-six metabolites were detected by nano-liquid chromatography coupled to a high-resolution electrospray-ionization quadrupole time-of-flight mass spectrometer (nLC-ESI-qTOF-MS) belonging to phenolic acids, coumarins, flavonoid glycosides, limonoids, terpenes, and fatty acids. Eleven metabolites were detected for the first time in Citrus sinensis and identified as citroside A, sinapic acid pentoside, apigenin-C-hexosyl-O-pentoside, chrysoeriol-C-hexoside, di-hexosyl-diosmetin, perilloside A, gingerol, ionone epoxide hydroxy-sphingenine, xanthomicrol, and coumaryl alcohol-O-hexoside. Some flavonoids were completely absent from the juice, while present most prominently in the Citrus peel, conveying more industrial and economic prospects to the latter. Multivariate data analyses clarified that the differences among orange parts overweighed the geographical source. PCA analysis of ESI-(−)-mode data revealed for hydroxylinoleic acid abundance in flavedo peel from Uruguay the most distant cluster from all others. The PCA analysis of ESI-(+)-mode data provided a clear segregation of the different Citrus sinensis parts primarily due to the large diversity of flavonoids and coumarins among the studied samples. Full article

Piperine (PN), the primary pungent alkaloid in black pepper shows several biological activities such as antioxidant, antimicrobial and anti-cancerogenic effects. Similar to other alkaloids, PN is characterized by poor water solubility. One way to improve its solubility and thus its biological activities is by forming inclusion complexes with suitable cyclodextrins. In this work PN inclusion complexes in native β-cyclodextrin (β-CD), its methylated (randomly methylated (RM-β-CD), heptakis-(2,6-di-O-methyl)-β-CD (DM-β-CD) and heptakis-(2,3,6-tri-O-methyl)-β-CD (TM-β-CD)) and 2-hydroxypropylated (HP-β-CD) derivatives are investigated using physicochemical methods, such as phase solubility study and X-ray crystallography complemented by theoretical (molecular dynamics simulations) studies. The determination of the crystal structure of the PN inclusion complexes in β-CD, DM-β-CD and TM-β-CD, reveals the formation of 1:2 guest:host inclusion complexes in the crystalline state. The guest PN molecule threads the hydrophobic cavities of the hosts which are arranged as couples in a tail-to-tail mode in the case of PN/β-CD and in a head-to-tail mode in the cases of PN/DM-β-CD and PN/TM-β-CD. MD studies based on the crystallographically determined structures and docked models show the stability of the examined complexes in an aqueous environment whereas the binding affinity of PN for the host molecules is calculated by the MM/GBSA method. Finally, phase-solubility studies of PN with β-CD, RM-β-CD and HP-β-CD are presented, indicating a B_s-type for the PN/β-CD complex and an A_L-type for the PN/RM-β-CD and PN/HP-β-CD complexes with 1:1 guest:host stoichiometry. Full article

In recent years, new solutions have been studied in order to intervene on existing buildings made of load-bearing masonry or reinforced concrete, both from the seismic point of view and the energy one. In recent years, novel modern techniques using lightweight steel profiles made of steel or aluminium alloys, suitably coupled with heat-insulating panels, have been proposed for requalification of existing building stock. In this paper, the Resisto 5.9 system designed by the Progetto Sisma S.r.l. company was studied and applied as a retrofit system of an existing masonry building. First, this new system for seismic energy requalification of the build-up was presented, describing the basic components and the mounting phases. Subsequently, it has applied to a case study building made of full-brick masonry located in the municipality of Casalecchio di Reno, on the outskirts of Bologna in Northern Italy. Finally, the used coating system was designed for the examined building together with other seismic interventions applied to horizontal structures and foundations. The seismic benefits deriving from using the proposed envelope system and other seismic interventions were proved from the seismic viewpoint by linear dynamic and non-linear static analyses using the TreMuri analysis software. From modal analysis it was seen that the dynamic behaviour of the building with Resisto 5.9 is improved, with an increase of the participating mass (68% instead of 49% of the bare building) of the second mode in the longitudinal direction. Moreover, from pushover analysis it was noticed that the seismic safety factor has passed from 0.51 to 1.05 in the X-direction and from 0.78 to 1.27 in the Y-direction. In conclusion, it was demonstrated that Resisto 5.9, together with all the other planned interventions, is able to accomplish the seismic retrofitting of the studied building. Full article

The structure–property correlations and control of electronic excited states in transition metal complexes (TMCs) are of high significance for TMC-based functional material development. Within these studies, a series of Re(I) carbonyl complexes with aryl-substituted 2,6-di(thiazol-2-yl)pyridines (Arⁿ-dtpy) was synthesized, and their ground- and excited-state properties were investigated. A number of condensed aromatic rings, which function as the linking mode of the aryl substituent, play a fundamental role in controlling photophysics of the resulting [ReCl(CO)₃(Arⁿ-dtpy-κ²N)]. Photoexcitation of [ReCl(CO)₃(Arⁿ-dtpy-κ²N)] with 1-naphthyl-, 2-naphthyl-, 9-phenanthrenyl leads to the population of ³MLCT. The lowest triplet state of Re(I) chromophores bearing 9-anthryl, 2-anthryl, 1-pyrenyl groups is ligand localized. The rhenium(I) complex with appended 1-pyrenyl group features long-lived room temperature emission attributed to the equilibrium between ³MLCT and ³IL/³ILCT. The excited-state dynamics in complexes [ReCl(CO)₃(9-anthryl-dtpy-κ²N)] and [ReCl(CO)₃(2-anthryl-dtpy-κ²N)] is strongly dependent on the electronic coupling between anthracene and {ReCl(CO)₃(dtpy-κ²N)}. Less steric hindrance between the chromophores in [ReCl(CO)₃(2-anthryl-dtpy-κ²N)] is responsible for the faster formation of ³IL/³ILCT and larger contribution of ³ILCT_{anthracene→dtpy} in relation to the isomeric complex [ReCl(CO)₃(9-anthryl-dtpy-κ²N)]. In agreement with stronger electronic communication between the aryl and Re(I) coordination centre, [ReCl(CO)₃(2-anthryl-dtpy-κ²N)] displays room-temperature emission contributed to by ³MLCT and ³IL_anthracene/³ILCT_{anthracene→dtpy} phosphorescence. The latter presents rarely observed phenomena in luminescent metal complexes. Full article

Tryptamine intoxications and fatalities are increasing, although these novel psychoactive substances (NPS) are not controlled in most countries. There are few data on the metabolic pathways and enzymes involved in tryptamine biotransformation. 4-acetoxy-N,N-diisopropyltryptamine (4-AcO-DiPT) is a synthetic tryptamine related to 4-hydroxy-N,N-diisopropyltryptamine (4-OH-DiPT), 4-acetyloxy-N,N-dipropyltryptamine (4-AcO-DPT), and 4-acetoxy-N,N-dimethyltryptamine (4-AcO-DMT). The aim of this study was to determine the best 4-AcO-DiPT metabolites to identify 4-AcO-DiPT consumption through human hepatocyte metabolism and high-resolution mass spectrometry. 4-AcO-DiPT metabolites were predicted in silico with GLORYx freeware to assist in metabolite identification. 4-AcO-DiPT was incubated with 10-donor-pooled human hepatocytes and sample analysis was performed with reversed-phase liquid chromatography coupled with high-resolution tandem mass spectrometry (LC-HRMS/MS) in positive- and negative-ion modes. Software-assisted LC-HRMS/MS raw data mining was performed. A total of 47 phase I and II metabolites were predicted, and six metabolites were identified after 3 h incubation following ester hydrolysis, O-glucuronidation, O-sulfation, N-oxidation, and N-dealkylation. All second-generation metabolites were derived from the only first-generation metabolite detected after ester hydrolysis (4-OH-DiPT). The metabolite with the second-most-intense signal was 4-OH-iPT-sulfate followed by 4-OH-DiPT-glucuronide, indicating that glucuronidation and sulfation are common in this tryptamine’s metabolic pathway. 4-OH-DiPT, 4-OH-iPT, and 4-OH-DiPT-N-oxide are suggested as optimal biomarkers to identify 4-AcO-DiPT consumption. Full article

This study focuses on the measurement of sub-23 nm particles emitted from a small DI/PFI spark ignition engine through conventional techniques and innovative systems. Measurements were performed with well-known systems, such as the EEPS coupled to a PMP-compliant sample conditioning device. Moreover, a novel instrument developed within the European project Sureal-23, the advanced HM-DMA, capable of operating with a simplified conditioning setup was used. The engine was fueled with ethanol, both pure and in blend at 30% v/v. The effects of fuel on the particle emissions were analyzed at different operating conditions. The results highlighted that a larger fraction of emissions consists of particles smaller than 23 nm, and their number changes according to the fuel, injection strategy and operating condition. A significant effect of the sampling system conditions was observed reveling the inception of nucleation mode particles or the condensation of the volatiles onto existing particles depending on the combination fuel/injection strategy. Different trends were noted at certain operating conditions between the results from the EEPS and the advanced HM-DMA ascribable to the different measurement principle and to the dilution system. Full article

Post-translational modifications by ubiquitin and ubiquitin-like proteins (Ubls) have known roles in a myriad of cellular processes. Ubiquitin- and Ubl-binding domains transmit the information conferred by these post-translational modifications by recognizing functional surfaces and, when present, different chain structures. Numerous domains binding to ubiquitin have been characterized and their structures solved. Analogously, motifs selectively interacting with SUMO (small ubiquitin-like modifier) have been identified in several proteins and their role in SUMO-dependent processes investigated. On the other hand, proteins that specifically recognize other Ubl modifications are known only in a few cases. The high sequence identity between NEDD8 and ubiquitin has made the identification of specific NEDD8-binding domains further complicated due to the promiscuity in the recognition by several ubiquitin-binding domains. Two evolutionarily related domains, called CUBAN (cullin-binding domain associating with NEDD8) and CoCUN (cousin of CUBAN), have been recently described. The CUBAN binds monomeric NEDD8 and neddylated cullins, but it also interacts with di-ubiquitin chains. Conversely, the CoCUN domain only binds ubiquitin. CUBAN and CoCUN provide an intriguing example of how nature solved the issue of promiscuity versus selectivity in the recognition of these two highly related molecules. The structural information available to date suggests that the ancestor of CUBAN and CoCUN was a three-helix bundle domain that diversified in KHNYN (KH and NYN domain-containing) and N4BP1 (NEDD4-binding protein-1) by acquiring different features. Indeed, these domains diverged towards two recognition modes, that recall respectively the electrostatic interaction utilized by the E3-ligase RBX1/2 in the interaction with NEDD8, and the hydrophobic features described in the recognition of ubiquitin by CUE (coupling ubiquitin conjugation to ER degradation) domains. Intriguingly, CUBAN and CoCUN domains are only found in KHNYN and N4BP1, respectively, both proteins belonging to the PRORP family whose members are characterized by the combination of protein modules involved in RNA metabolism with domains mediating ubiquitin/NEDD8 recognition. This review recapitulates the current knowledge and recent findings of CUBAN and CoCUN domains and the proteins containing them. Full article

Carbon monoxide (CO) is a commonly poisonous gas. It is important to detect CO in daily life. Herein, a new and sensitive surface enhanced Raman scattering (SERS) and resonance Rayleigh scattering (RRS) coupled di-mode method was developed for CO, based on gold nano-enzyme catalysis and gold nanoprobes. CO can react with HAuCl₄ to generate gold nanoparticles (AuNPs) in pH 5.2 HAc-NaAc buffer. The generated AuNPs exhibited SERS activity at 1620 cm⁻¹ in the presence of Vitoria blue B (VBB) molecular probes, and an RRS peak at 290 nm. Based on the AuNP bifunctional probes, the increased SERS and RRS intensities respond linearly with the concentration of CO in the range of 100–1500 ng/mL and 30–5230 ng/mL, respectively. To improve the sensitivity, the produced AuNPs were used as nano-enzyme catalysts for the new indicator reaction of HAuCl₄-ethanol (En) to amplify the signal. The sensitive SERS method was coupled with the accurate RRS method to develop a sensitive and accurate SERS/RRS di-mode method for determination of 3.0–413 ng/mL CO, based on the AuNP-HAuCl₄-En nanocatalytic reaction and its product of AuNPs as SERS and RRS bifunctional probes. Full article

To perform the fault protection for the microgrid in grid-connected mode, the wavelet energy fuzzy neural network-based technique (WEFNNBT) is proposed in this paper. Through the accurate activation of protective relay, the microgrid can be effectively isolated from the utility power system to prevent serious voltage fluctuation when the power quality of power system is disturbed. The proposed WEFNNBT can be divided into three stages—feature extraction (FE), feature condensation (FC), and disturbance identification (DI). In the FE stage, the feature of power signal at the point of common coupling (PCC) between microgrid and utility power system would be extracted with discrete wavelet transform (DWT). Then, the wavelet energy and variation of singular power signal can be obtained according to Parseval Theorem. To determine the dominant wavelet energy and enhance the robustness to the noise, the feature information is integrated in the FC stage. The feature information then would be processed in the DI stage to perform the fault identification and activate the protective relay if necessary. From the experimental results, it is realized that the proposed WEFNNBT can effectively perform the fault protection of microgrid. Full article

This study aims to construct a reduced thermodynamic cycle model with high accuracy and high model execution speed based on artificial neural network training for real-time numerical analysis. This paper proposes a method of constructing a fast average-value model by combining a 1D plant model and exhaust gas recirculation (EGR) control logic. The combustion model of the detailed model uses a direct-injection diesel multi-pulse (DI-pulse) method similar to diesel combustion characteristics. The DI-pulse combustion method divides the volume of the cylinder into three zones, predicting combustion- and emission-related variables, and each combustion step comprises different correction variables. This detailed model is estimated to be within 5% of the reference engine test results. To reduce the analysis time while maintaining the accuracy of engine performance prediction, the cylinder volumetric efficiency and the exhaust gas temperature were predicted using an artificial neural network. Owing to the lack of input variables in the training of artificial neural networks, it was not possible to predict the 0.6–0.7 range for volumetric efficiency and the 1000–1200 K range for exhaust gas temperature. This is because the mean value model changes the fuel injection method from the common rail fuel injection mode to the single injection mode in the model reduction process and changes the in-cylinder combustion according to the injection timing of the fuel amount injected. In addition, the mean value model combined with EGR logic, i.e., the single-input single-output (SISO) coupled mean value model, verifies the accuracy and responsiveness of the EGR control logic model through a step-transient process. By comparing the engine performance results of the SISO coupled mean value model with those of the mean value model, it is observed that the SISO coupled mean value model achieves the desired target EGR rate within 10 s. The EGR rate is predicted to be similar to the response of volumetric efficiency. This process intuitively predicted the main performance parameters of the engine model through artificial neural networks. Full article

The high dv/dt and di/dt outputs from power devices in a high-low voltage DC-DC converter on electric vehicles (EVs) can always introduce the unwanted conducted electromagnetic interference (EMI) emissions. A conducted EMI prediction and mitigation strategy that is based on transfer function for the high-low voltage DC-DC converter in EVs are proposed. A complete test for the DC-DC converter is conducted to obtain the conducted EMI from DC power cables in the frequency band of 150 kHz-108 MHz. The equivalent circuit with high-frequency parasitic parameters of the DC-DC converter is built`1 based on the measurement results to acquire the characteristics of the conducted EMI of the DC power cables. The common mode (CM) and differential mode (DM) propagation coupling paths are determined, and the corresponding transfer functions of the DM interference and CM interference are established. The simulation results of the conducted EMI can be obtained by software Matlab and Computer Simulation Technology (CST). By analyzing the transfer functions and the simulation results, the dominated interference is the CM interference, which is the main factor of the conducted EMI. A mitigation strategy for the design of the CM interference filter based on the dominated CM interference is proposed. Finally, the mitigation strategy of the conducted EMI is verified by performing the conducted voltage experiment. From the experiment results, the conducted voltage of the DC power cables is decreased, respectively, by 58 dBμV, 55 dBμV, 65 dBμV, 53 dBμV, and 54 dBμV at frequency 200 kHz, 400 kHz, 600 kHz, 1.4 MHz, and 50 MHz. The conduced voltage in the frequency band of 150 kHz–108 MHz can be mitigated by adding the CM interference filters, and the values are lower than the limit level-3 of CISPR25 standard (GB/T 18655-2010). Full article

In this work, flavonoid fraction from the leaves of Crataegus pinnatifida was separated into its seven main constituents using a combination of HSCCC coupled with pre-HPLC. In the first step, the total flavonoid extract was subjected to HSCCC with a two-solvent system of chloroform/methanol/water/n-butanol (4:3:2:1.5, v/v), yielding four pure compounds, namely (–)-epicatechin (1), quercetin-3-O-(2,6-di-α-l-rhamnopyranosyl)-β-d-galactopyranoside (2), 4′′-O-glucosylvitexin (3) and 2′′-O-rhamnosylvitexin (4) as well as a mixture of three further flavonoids. An extrusion mode was used to rapidly separate quercetin-3-O-(2,6-di-α-l-rhamnopyranosyl)-β-d-galactopyranoside with a big K_D-value. In the second step, the mixture that resulted from HSCCC was separated by pre-HPLC, resulting in three pure compounds including: vitexin (5), hyperoside (6) and isoquercitrin (7). The purities of the isolated compounds were established to be over 98%, as determined by HPLC. The structures of these seven flavonoids were elucidated by ESI-MS and NMR spectroscopic analyses. Full article