Search Results (82)

Anthropogenic ponds have the potential to shape the post-industrial landscape and mitigate the effects of climate change, particularly in urban heat island-threatened areas. However, decisions regarding their inclusion in blue–green infrastructure networks require balancing costs and benefits while considering potential pollution risks. The objectives of this study are: (i) to develop an efficient decision-making framework based on standard aquatic science tools; (ii) to apply this framework to a specific artificial pond in the Upper Silesian Industrial Region, Poland, in order to optimize actions based on resources, advantages, limitations, and informativeness of the data. Eighteen methods, grouped into five categories, including historical document analyses, hydroacoustic and modeling methods, multiparametric water quality measurements, and ecotoxicological tests, were used. Optimization-focused analysis indicated that investigating historical documents should precede further testing, as it enables decision-makers to select the most effective methods to assess the pond’s value for blue–green infrastructure. In this case, the tests based on metal pollution, bathymetry, and biodiversity appeared sufficient. The presented approach offers a straightforward screening method for assessing reservoirs in post-industrial areas. Full article

Odonata constitute an important invertebrate group that is strongly dependent on water conditions and sensitive to habitat disturbances, rendering them reliable indicators of habitat quality of both aquatic and terrestrial habitats. We studied the compositional and diversity patterns of Odonates in total, and separately for the two suborders (Zygoptera, Anisoptera) in relation to geographic and ecological parameters at the riparian zone of four rivers and one canal within the Axios Delta National Park and the Natura 2000 SAC GR1220002 in northern Greece, using the line transect technique. In total, 6252 individuals belonging to 28 species were identified. The compositional and diversity patterns were significantly different between agricultural and natural sites. Odonata assemblages at croplands were comparatively poorer, dominated by a few, widely distributed, taxonomically proximal species, tolerant to environmental changes, as a result of modifications and consequent alterations of abiotic conditions at croplands, which also led to higher local contribution to β-diversity and species turnover. The absence of several percher, endophytic, and threatened species from agricultural sites led to significantly lower diversity, as a result of environmental filtering due to ecophysiological restrictions. Taxonomic and functional diversity, uniqueness, and Dragonfly Biotic Index (DBI) were significantly higher in riparian forests, due to the sensitivity of damselflies to dehydration, and the avoidance of habitat loss and extreme temperatures by dragonflies, which prefer natural shelters near the ecotone. The newly introduced Conservation Value Index (CVI) revealed 21 conservation hotspots of Odonata (14 at canopy cover sites), widely distributed within the borders of NATURA 2000 SAC GR1220002. Full article

It is projected that the world population will quadruple over the next century, and to meet future food demands, agricultural production will need to increase by 70%. Therefore, there has been a transition from traditional farming methods to autonomous modern agriculture. One such modern technique is aeroponic farming, in which plants are grown without soil under controlled and hygienic conditions. In aeroponic farming, plants are significantly less affected by climatic conditions, infectious diseases, and biotic and abiotic stresses, such as pest infestations. Additionally, this method can reduce water, nutrient, and pesticide usage by 98%, 60%, and 100%, respectively, while increasing the yield by 45–75% compared to traditional farming. In this study, a three-dimensional industrial design of an innovative aeroponic plant growth chamber was presented for use by individuals, researchers, and professional growers. The proposed chamber design is modular and open to further innovation. Unlike existing chambers, it includes load cells that enable real-time monitoring of the fresh weight of the plant. Furthermore, cameras were integrated into the chamber to track plant growth and changes over time and weight. Additionally, RGB power LEDs were placed on the inner ceiling of the chamber to provide an optimal lighting intensity and spectrum based on the cultivated plant species. A customizable chamber design was introduced, allowing users to determine the growing tray and nutrient nozzles according to the type and quantity of plants. Finally, system models were developed for temperature control of the chamber. Temperature control was implemented using a proportional-integral-derivative controller optimized with particle swarm optimization, radial movement optimization, differential evolution, and mayfly optimization algorithms for the gain parameters. The simulation results indicate that the temperatures of the growing and feeding chambers in the cabinet reached a steady state within 260 s, with an offset error of no more than 0.5 °C. This result demonstrates the accuracy of the derived model and the effectiveness of the optimized controllers. Full article

Water temperature is a fundamental parameter influencing a range of biotic and abiotic processes occurring within various components of the hydrosphere. This study presents a multi-step, data-driven predictive modeling framework to estimate water temperatures for the period 2021–2100 in three aquatic environments in Central Europe: the Odra River, the Szczecin Lagoon, and the Baltic Sea. The framework integrates Bayesian Model Averaging (BMA), Random Sample Consensus (RANSAC) regression, Gradient Boosting Regressor (GBR), and Random Forest (RF) machine learning models. To assess the performance of the models, the coefficient of determination (R2), mean absolute error (MAE), and root mean square error (RMSE) were used. The results showed that the application of statistical downscaling methods improved the prediction of air temperatures with respect to the BMA. Moreover, the RF method was used to predict water temperature. The best model performance was obtained for the Baltic Sea and the lowest for the Odra River. Under the SSP2-4.5 and SSP5-8.5 scenario-based simulations, projected air temperature increases in the period 2021–2100 could range from 1.5 °C to 1.7 °C and 4.7 to 5.1 °C. In contrast, the increase in water temperatures by 2100 will be between 1.2 °C and 1.6 °C (SSP2-4.5 scenario) and between 3.5 °C and 4.9 °C (SSP5-8.5). Full article

Tomato is a major crop, and efforts are ongoing to enhance its resilience to biotic and abiotic stresses. Weed management remains a key challenge, prompting the search for sustainable alternatives to reduce the impact of excessive herbicide use. Biochar is a promising alternative, as it enriches the soil, improves its water retention capacity, promotes its regeneration and increased fertility, delays nutrient leaching, and improves fertilizer use efficiency. This study aimed to investigate the efficiency of biochar use in mitigating stress caused by different herbicides. Two different biochar materials, Douglas fir and rice husk, were used. Tomato seeds were sown in pots and arranged in a randomized design. At the 4V stage (28 days after sowing), the herbicides S-metolachlor, metribuzin, and halosulfuron were applied. Plant length, injury, antioxidant enzyme activity, ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPOD), glutathione reductase (GR), and hydrogen peroxide content (H₂O₂) were assessed 7 and 14 days after herbicide application. Plants treated with biochar and submitted to herbicide treatments showed significantly higher growth parameters and fewer injuries when compared to plants treated with herbicides without biochar. The antioxidant response of the plants followed the same trend; smaller plants with more injuries showed greater H₂O₂ accumulation and significantly higher antioxidant enzyme activity. These findings highlight the protective effect of biochar, particularly Douglas fir biochar, as it effectively mitigated herbicide-induced oxidative stress and helped maintain plant growth and structural integrity under treatment conditions. Full article

Lake Maggiore is a deep subalpine lake that has been well studied since the last century thanks to a monitoring program funded by the International Commission for the Protection of Italian–Swiss Waters. The monitoring program comprises both abiotic and biotic parameters, including zooplankton pelagic organisms. In this study, we present a dataset of 15,563 records of population densities and standing stock biomass for zooplankton pelagic taxa recorded over 43 years (1981–2023). The long-term dataset is valuable for tracing changes in trophic conditions experienced by the lake during the last century (eutrophication and its reversal) and the impact of global warming. Zooplankton samples (Crustacea and Rotifera Monogononta) were collected within 0–50 m depth by vertical hauls with an 80 µm light plankton sampler. The sampling frequency was monthly, with the exception of the 2009–2012 period, which employed seasonal frequency. The estimation of zooplankton taxon abundance and of its standing stock biomass is crucial in order to quantify the flux of matter, energy, and pollutants up to the upper trophic levels of the food web. The dataset provided is also suitable for food web analysis because the zooplankton taxa have been classified according to their ecological roles (microphagous organisms; primary and secondary consumers). Full article

Forests are complex systems in which subtle variations in terrain can reveal much about plant community structure and interspecific interactions. Despite a wealth of studies focusing on broad-scale environmental gradients, the role of fine-scale topographic nuances often remains underappreciated, particularly in subtropical settings. In our study, we explore how minute differences in microtopography—encompassing local elevation, slope, aspect, terrain position index (TPI), terrain ruggedness index (TRI), and flow direction—affect neighborhood-scale interactions among plants. We established an 11.56-hectare dynamic plot in a subtropical forest at the northern margin of China’s subtropical zone, where both microtopographic factors and neighborhood indices (density, competition, diversity) were systematically measured using 5 m × 5 m quadrats. Parameter estimation and mixed-effects models were employed to examine how microtopography influences plant spatial patterns, growth, and competitive dynamics across various life stages. Our findings demonstrate that aspect and TPI act as key drivers, redistributing light and moisture to shape conspecific clustering, heterospecific competition, and tree growth. Remarkably, sun-facing slopes promoted sapling aggregation yet intensified competitive interactions, while shaded slopes maintained stable moisture conditions that benefited mature tree survival. Moreover, in contrast to broader-scale observations, fine-scale TRI was associated with reduced species richness, highlighting scale-dependent heterogeneity effects. The intensification of plant responses with life stage indicates shifting resource demands, where light is critical during early growth, and water becomes increasingly important for later survival. This study thus advances our multiscale understanding of forest dynamics and underscores the need to integrate fine-scale abiotic and biotic interactions into conservation strategies under global change conditions. Full article

The color of the plant leaves is a major concern in many areas of agriculture. Pigmentation and its pattern provide the possibility to distinguish genotypes and a basis for annual crop management practices. For example, the nutrient and water status of plants is reflected in the chlorophyll content of leaves that are strongly linked to the lamina coloration. Pests and diseases (virus or bacterial infections) also cause symptoms on the foliage. These symptoms induced by biotic and abiotic stressors often have a specific pattern, which allows for their prediction based on remote sensing. In this report, an RGB (red, green and blue) image processing system is presented to determine leaf lamina color variability based on RGB-based color indices. LeafLaminaMap was developed in Scilab with the Image Processing and Computer Vision toolbox, and the code is available freely at GitHub. The software uses RGB images to visualize 29 color indices and the R, G and B values on the lamina, as well as to calculate the statistical parameters. In this case study, symptomatic (senescence, fungal infection, etc.) and healthy grapevine (Vitis vinifera L.) leaves were collected, digitalized and analyzed with the LeafLaminaMap software according to the mean, standard deviation, contrast, energy and entropy of each channel (R, G and B) and color index. As an output for each original image in the sample set, the program generates 32 images, where each pixel is constructed using index values calculated from the RGB values of the corresponding pixel in the original image. These generated images can subsequently be used to help the end-user identify locally occurring symptoms that may not be visible in the original RGB image. The statistical evaluation of the samples showed significant differences in the color pattern between the healthy and symptomatic samples. According to the F value of the ANOVA analysis, energy and entropy had the largest difference between the healthy and symptomatic samples. Linear discriminant analysis (LDA) and support vector machine (SVM) analysis provided a perfect recognition in calibration and confirmed that energy and entropy have the strongest discriminative power between the healthy and symptomatic samples. The case study showed that the LeafLaminaMap software is an effective environment for the leaf lamina color pattern analysis; moreover, the results underline that energy and entropy are valuable features and could be more effective than the mean and standard deviation of the color properties. Full article

Biostimulants include a wide array of microorganisms and substances that can exert beneficial effects on plant development and growth, often enhancing nutrient uptake and improving tolerance against abiotic and biotic stress. Depending on their composition and time of application, these products can influence plant physiology directly as growth regulators or indirectly through environmental condition changes in the rhizosphere, such as nutrient and water availability. This review evaluated 48 case studies from 39 papers to summarize the effects of biostimulant application on fruit and tuber yields and on the quality of processing tomato and potato in open field conditions. For potato, PGPR bacteria were the main studied biostimulant, whereas the low number of studies on processing tomato did not permit us to delineate a trend. The yield and quality were greatly influenced by cultivars and biostimulant composition, application method, period, and dose. For processing tomato, a positive effect of the biostimulant application on the marketable yield was reported in 79% of the case studies, whereas for potato, the effect was reported in only 47%. Few studies, on processing tomato and potato, also reported data for quality parameters with contrasting results. The variability of crop response to biostimulant application in open field conditions highlights the need for more comprehensive studies. Such studies should focus on diverse cultivars, deeply understand the interaction of biostimulant application with agronomic management (e.g., irrigation and fertilization), and evaluate yield and quality parameters. This approach is crucial to fully understand the potential and limitations of biostimulant applications in agriculture, particularly regarding their role in sustainable crop production. Full article

Seasonal variations of drainage waters and ochreous products of their discharge from the closed abandoned old gallery at the Grantcharitsa scheelite deposit (Bulgaria) were studied by field and laboratory methods for the period 2019–2023. The drainage is generated under anoxic conditions and is inherently diluted (EC = 100–202 µS/cm) with S (6–12 mg/L), Si (6–22 mg/L), Na (6–10 mg/L), Fe (0.2–3.3 mg/L), and W (0.19–3.5 µg/L), at a pH 4.4–6.5 and temperature 7–11.5 °C, with dissolved oxygen DO (2.1–7.7 mg/L). The concentrations of Fe and W and the pH of the water are variable and reach their maximum values during the dry (autumn) season. It was found that such parameters as pH, Eh, DO, Fe and W content change dramatically at a distance of up to 3 m from the water outlet; the values of pH, DO and Eh are sharply increased with a simultaneous nearly 5–6-times reduction in iron and tungsten content. The decrease in the contents of these elements is associated with the precipitation of ochreous material consisting of nanoscale ferrihydrite with an intermediate structural ordering between 2-line and 6-line ferrihydrite (major phase), hematite, goethite, quartz, montmorillonite and magnetite. The formation of ferrihydrite occurs as a result of abiotic and biotic processes with the participation of iron-oxidizing bacteria. Besides Fe₂O₃ (55.5–64.0 wt.%), the ochreous sediment contains SiO₂ (12.0–16.4 wt.%), SO₃ (1.3–2.4 wt.%), Al₂O₃ (3.1–6.8 wt.%) and WO₃ (0.07–0.11 wt.%). It has been shown that drainage waters and ochreous sediments do not inherently have a negative impact on the environment. The environmental problem arises with intense snowmelt and heavy rainfall, as a result of which the accumulated sediments are washed away and carried in the form of suspensions into the water systems. It is suggested that by providing atmospheric oxygen access to the closed gallery (via local boreholes), it is possible to stop the generation of iron-enriched drainage. Full article

In an era when ecological and environmental needs and responsibilities apply pressure on the world’s countries and sustainability takes centre stage, ecologic/environmental (E/E) laboratories stand as beacons of scientific inquiry, innovating, optimising, and applying various tests for a better knowledge of our natural resources and the quality status of ecosystems. The purpose of this review is to provide an overview of the use of flow cytometry (FC) as a tool for assessing environmental quality, mainly using living organisms and their biological changes as bioindicators. Cytometric approaches applied to both marine and terrestrial ecosystems ensure the detection of biochemical and functional status of the cells composing either an organ thereof or the organism itself. In addition to cytometric evaluations of the biotic matrix, a brief overview of the techniques for the environmental assessment of biotic and abiotic matrices using mass spectrometry is given. The technique involving the continuous monitoring of the chemical and physical parameters of water, sediment, and soil is basically incapable of detecting any additive and synergetic effects of toxicants on living organisms. Therefore, techniques employing bioindicators provide valuable information for environmental diagnosis, and several studies have demonstrated the strong relationship between specific environmental data and cell/organ behaviour. Full article

Current sampling methods for detecting the presence of the invasive freshwater hydrozoan Craspedacusta sowerbii rely mainly on visual confirmation of the medusa stage. Confirming the presence of the polyp stage is equally important for observing medusae since typical late summer/early fall occurrences or observations of medusae are sporadic though are becoming more frequent. The polyp stage is important as it is the organism’s primary stage and is present throughout the year depending on water temperatures. Therefore, sampling methods for the polyp stage are, commonly, the collection of substrates such as rocks, plants, or pieces of wood in a given body of water, and these can be cumbersome to examine. Polyps are also small, transparent, and difficult to see on natural substrates. Based on a preliminary culturing of the polyp stage on glass and plastic microscope slides in the laboratory, we designed a sampling methodology based on submerging four substrate types (glass and plastic microscope slides, Hester-Dendy discs, and small glass Petri dishes) to confirm the presence of C. sowerbii polyps in the field. We tested this method in three lakes in the Illinois–Indiana region (USA). Two of the lakes have recorded sightings of medusae but the third has no record of polyps or medusae. The sampling method we designed was effective in that C. sowerbii polyps were found on both plastic and glass slides. While this method can be sufficient for detection of the polyp stage, it also shows potential for improvement; we highlight abiotic and biotic ecological parameters as significant factors influencing the collection of C. sowerbii polyps to be considered for future methodologies. Full article

The fungi Botryosphaeriaceae are involved in olive declines in both the world hemispheres and in all continents where this species is cultivated. In Salento (Apulia, Italy), the Botryosphaeriaceae Neofusicoccum mediterraneum and N. stellenboschiana have been reported as the agents of a branch and twig dieback that overlaps with olive quick decline syndrome caused by Xylella fastidiosa subsp. pauca. In this study, we report the finding of Diplodia seriata, another Botryosphaeriaceae species, in Salento in Xylella fastidiosa-infected olive trees affected by symptoms of branch and twig dieback. Given that its presence was also reported in olive in the Americas and in Europe (Croatia) with different degrees of virulence, we were prompted to assess its role in the Apulian decline. We identified representative isolates based on morphological features and a multilocus phylogeny. In vitro tests showed that the optimum growth temperature of the isolates is around 25–30 °C, and that they are highly thermo-tolerant. In pathogenicity trials conducted over eleven months, D. seriata expressed a very low virulence. Nonetheless, when we imposed severe water stress before the inoculation, D. seriata significatively necrotized bark and wood in a time frame of 35 days. Moreover, the symptoms which resulted were much more severe in the trial performed in summer compared with that in autumn. In osmolyte-supplemented media with a water potential from −1 to −3 Mpa, the isolates increased or maintained their growth rate compared with non-supplemented media, and they also grew, albeit to a lesser extent, on media with a water potential as low as −7 Mpa. This suggests that olives with a low water potential, namely those subjected to drought, may offer a suitable environment for the fungus’ development. The analysis of the meteorological parameters, temperatures and rainfall, in Salento in the timeframe 1989–2023, showed that this area is subjected to a progressive increase of temperature and drought during the summer. Thus, overall, D. seriata has to be considered a contributor to the manifestation of branch and twig dieback of olive in Salento. Coherently with the spiral decline concept of trees, our results suggest that heat and drought act as predisposing/inciting factors facilitating D. seriata as a contributor. The fact that several adverse factors, biotic and abiotic, are simultaneously burdening olive trees in Salento offers a cue to discuss the possible complex nature of the olive decline in Salento. Full article

Currently, different strategies, including the application of bio-fertilizers, are used to ameliorate the adverse effects posed by salinity stress as the major global problem in plants. Fish waste is suggested as a novel bio-fertilizer to mitigate the effects of biotic and abiotic stresses. In this investigation, an experiment was conducted to investigate the effects by applying different concentrations (0, 5, 10, and 15% (v/v)) of fish waste bio-fertilizer on stevia plants grown under salt stress conditions (0, 20, 40, and 60 mM of NaCl). Results showed that salinity negatively affected growth parameters, the photosynthetic pigments, the relative water content, and the chlorophyll fluorescence parameters while increased the activity of antioxidant enzymes, total phenol, hydrogen peroxide (H₂O₂), malondialdehyde (MDA), proline, and total carbohydrates compared with control samples. On the other hand, the application of fish waste bio-fertilizer mitigated the effects of salinity stress by enhancing growth and mitigating stress-relative markers, especially at the highest salinity level (60 mM). Overall, fish waste bio-fertilizer could be considered a sustainable, innovative approach for the alleviation of salinity stress effects in plants and, in addition, fish waste bio-fertilizer did not cause more salinity issues, at least with the applied doses and experiment time, which is an imperative aspect. Full article

Tomato production in the Canary Islands has significantly decreased in recent years due to the presence of parasites and pathogens, poor-quality irrigation water, lack of infrastructure modernization, and increased competition. To address this issue, local varieties with better agro-climatic adaptation and organoleptic characteristics have been cultivated. These varieties show their maximum potential under an agro-ecological cultivation system, where the beneficial micro-organisms of the rhizosphere (in general) and mycorrhizal fungi (in particular) have a positive influence on their development, especially when the plants are subjected to biotic or abiotic stresses. Irrigation water in Canary Islands tomato cultivation comes from groundwater sources with moderate levels of sodium and chlorides or sodium and bicarbonates. This study evaluated the response of mycorrizal plants of the local tomato variety “Manzana Negra” under abiotic stress conditions due to the presence of chlorides and bicarbonates. Two tests were carried out with mycorrhizal and non-mycorrhizal plants. In the first one, 0, 75, and 150 mM NaCl solutions were applied. In the second, the nutrient solution was enriched with sodium bicarbonate at doses of 0, 2.5, 5, 7.5, 10, and 12.5 mM. Presence of native mycorrhizae improved the growth and nutrition of plants affected by irrigation with saline and alkaline water containing chloride and sodium carbonate. Symbiosis produced statistically significant increases in all plant-development-related variables (stem length and diameter; fresh and dry weight) in all bicarbonate concentrations. However, the results with the application of sodium chloride do not seem to indicate a positive interaction in most of the analytical parameters at 150 mM NaCl concentration. The mycorrhizal inoculation with local fungi can be interesting in the production of seedlings of this tomato variety in situations of moderate salinity, especially under bicarbonate stress conditions. Full article