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Keywords = acid erosion diversion

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17 pages, 3431 KB  
Review
Conservation and Sustainable Development of Rice Landraces for Enhancing Resilience to Climate Change, with a Case Study of ‘Pantiange Heigu’ in China
by Shuyan Kou, Zhulamu Ci, Weihua Liu, Zhigang Wu, Huipin Peng, Pingrong Yuan, Cheng Jiang, Huahui Li, Elsayed Mansour and Ping Huang
Life 2026, 16(1), 143; https://doi.org/10.3390/life16010143 - 15 Jan 2026
Abstract
Climate change poses a threat to global rice production by increasing the frequency and intensity of extreme weather events. The widespread cultivation of genetically uniform modern varieties has narrowed the genetic base of rice, increasing its vulnerability to these increased pressures. Rice landraces [...] Read more.
Climate change poses a threat to global rice production by increasing the frequency and intensity of extreme weather events. The widespread cultivation of genetically uniform modern varieties has narrowed the genetic base of rice, increasing its vulnerability to these increased pressures. Rice landraces are traditional rice varieties that have been cultivated by farming communities for centuries and are considered crucial resources of genetic diversity. These landraces are adapted to a wide range of agro-ecological environments and exhibit valuable traits that provide tolerance to various biotic stresses, including drought, salinity, nutrient-deficient soils, and the increasing severity of climate-related temperature extremes. In addition, many landraces possess diverse alleles associated with resistance to biotic stresses, including pests and diseases. In addition, rice landraces exhibit great grain quality characters including high levels of essential amino acids, antioxidants, flavonoids, vitamins, and micronutrients. Hence, their preservation is vital for maintaining agricultural biodiversity and enhancing nutritional security, especially in vulnerable and resource-limited regions. However, rice landraces are increasingly threatened by genetic erosion due to widespread adoption of modern high-yielding varieties, habitat loss, and changing farming practices. This review discusses the roles of rice landraces in developing resilient and climate-smart rice cultivars. Moreover, the Pantiange Heigu landrace, cultivated at one of the highest altitudes globally in Yunnan Province, China, has been used as a case study for integrated conservation by demonstrating the successful combination of in situ and ex situ strategies, community engagement, policy support, and value-added development to sustainably preserve genetic diversity under challenging environmental and socio-economic challenges. Finally, this study explores the importance of employing advanced genomic technologies with supportive policies and economic encouragements to enhance conservation and sustainable development of rice landraces as a strategic imperative for global food security. By preserving and enhancing the utilization of rice landraces, the agricultural community can strengthen the genetic base of rice, improve crop resilience, and contribute substantially to global food security and sustainable agricultural development in the face of environmental and socio-economic challenges. Full article
(This article belongs to the Section Plant Science)
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13 pages, 1169 KB  
Article
The Selective Extraction of Natural Sesquiterpenic Acids in Complex Matrices: A Novel Strategy for Isolating Zizanoic Acid in Vetiver Essential Oil
by Ian Gardel Carvalho Barcellos-Silva, Ananda da Silva Antonio, Mateus Curty Cariello da Silva, Fernanda de Melo Regazio Cariello, Fernando Hallwass, Monica Costa Padilha and Valdir Florencio Veiga-Junior
Separations 2025, 12(6), 163; https://doi.org/10.3390/separations12060163 - 17 Jun 2025
Viewed by 1030
Abstract
Essential oils are complex mixtures of apolar components, mainly phenylpropanoids, monoterpenes, and sesquiterpenes. Vetiver (Vetiveria zizanioides (L.) Nash) is a non-endemic grass in several tropical regions, widely used for slope stabilization and erosion control because of its long and deep roots that [...] Read more.
Essential oils are complex mixtures of apolar components, mainly phenylpropanoids, monoterpenes, and sesquiterpenes. Vetiver (Vetiveria zizanioides (L.) Nash) is a non-endemic grass in several tropical regions, widely used for slope stabilization and erosion control because of its long and deep roots that help to bind the soil together, preventing landslides and soil loss. From these roots, vetiver essential oil is obtained, which is extracted and produced worldwide and highly valued for its diverse range of bioactive substances used by the cosmetics and perfume industries. These substances, present in a very complex mixture, are difficult to isolate. Zizanoic acid is a very rare substance in nature and also very interesting because of the biological properties already described. In the present study, zizanoic acid was selectively isolated with 84–87% purity from vetiver commercial essential oils, in which it was present at less than 10%, using KOH-impregnated silica gel column chromatography alone. The experiments were monitored using GC-MS and UHPLC-HRMS, and the isolated substances (zizanoic and valerenic acids) were further determined by NMR experiments. The whole methodology and analytical approach proved to be very efficient for natural product complex mixture analysis and also very selective, allowing for a distinct capacity to recover carboxylic acids from complex biological samples. Full article
(This article belongs to the Special Issue Extraction and Characterization of Food Components)
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23 pages, 6820 KB  
Article
Anti-Erosion Mechanism of Biological Crusts and Eco-Protection Technology Using Composite Biofilms for Traditional Rammed Earth Dwellings in Songyang County
by Jiahui Yang, Ning Wang, Zebiao Huang, Yue Huang, Weilu Lv and Shuai Yang
Coatings 2025, 15(5), 608; https://doi.org/10.3390/coatings15050608 - 20 May 2025
Cited by 2 | Viewed by 1202
Abstract
A typical county for traditional village conservation in China is Songyang County. It is renowned for its ancient rammed earth dwellings, which exhibit a unique microclimate and possess significant historical value. However, high precipitation and acid rain under the subtropical monsoon climate have [...] Read more.
A typical county for traditional village conservation in China is Songyang County. It is renowned for its ancient rammed earth dwellings, which exhibit a unique microclimate and possess significant historical value. However, high precipitation and acid rain under the subtropical monsoon climate have caused severe surface erosion, including cracking and spalling. This study focuses on traditional rammed earth dwellings in Chenjiapeng Village, Songyang County, combining field surveys, experimental analysis, and microscopic characterization to systematically investigate erosion mechanisms and protection strategies. Techniques, such as drone aerial photography, X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM), and microbial diversity detection, were employed to elucidate the anti-erosion mechanisms of gray–green biological crusts on rammed earth surfaces. The results indicate that algal crusts enhance surface compressive strength and shear resistance through macroscopic coverage (reducing raindrop kinetic energy and moisture retention) and microscopic extracellular polysaccharide-cemented soil particles forming a three-dimensional network. However, acidic environments induce metabolic acid release from algae, dissolving cementing materials and creating a “surface protection-internal damage” paradox. To address this, a “transparent film-biofiber-acid inhibition layer” composite biofilm design is proposed, integrating a biodegradable polylactic acid (PLA) mesh, algal attachment substrates, and calcium carbonate microparticles to dynamically neutralize acidic substances, achieving synergistic ecological protection and cultural heritage authenticity. This study provides innovative solutions for the anti-erosion protection of traditional rammed earth structures, emphasizing environmental compatibility and sustainability. Full article
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15 pages, 5404 KB  
Article
Effectiveness of Pre-Sowing Treatments on Seed Germination of Nine Acacia Species from Al-Baha Region in Saudi Arabia
by Ali A. Alzandi, Ibrahim M. Aref and Nels Grevstad
Seeds 2025, 4(2), 22; https://doi.org/10.3390/seeds4020022 - 30 Apr 2025
Viewed by 2061
Abstract
Acacia species are important trees in arid ecosystems due to their diverse ecological roles, such as providing vegetation cover, community structures, food resources for animals, soil stabilization, and erosion prevention. However, in the Arabian Peninsula, Acacia species are declining due to climate change, [...] Read more.
Acacia species are important trees in arid ecosystems due to their diverse ecological roles, such as providing vegetation cover, community structures, food resources for animals, soil stabilization, and erosion prevention. However, in the Arabian Peninsula, Acacia species are declining due to climate change, overgrazing, and fuelwood harvesting. This study evaluates the effectiveness of various pre-sowing treatments—sulfuric acid soaking and tap and hot water soaking—on breaking seed dormancy to enhance germination in nine Acacia species native to the Al-Baha region of Saudi Arabia. The key germination indicators assessed were the mean germination time (MGT), germination percentage (GP), and germination index (GI). Sulfuric acid treatments for 10–15 min reduced the MGT and increased the GP for A. etbaica, A. hamoulosa, and A. tortilis, while A. origena responded best to 1 min of hot water soaking. Conversely, A. asak, A. ehrenbergiana, and A. johnwoodii showed little to no germination improvement with treatment and A. oerfota and A. gerrardii showed no germination improvement, indicating the need for alternative methods. These findings indicate that the seed germination requirements vary within Acacia spp. from the same geographic region and similar climatic conditions. Further work is required for five of the species tested to develop better seed germination techniques, given the potential utility of Acacia spp., in ecological restoration and sustainable land management in arid regions. Full article
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23 pages, 29777 KB  
Article
Monitoring and Prevention Strategies for Iron and Aluminum Pollutants in Acid Mine Drainage (AMD): Evidence from Xiaomixi Stream in Qinling Mountains
by Xiaoya Wang, Min Yang, Huaqing Chen, Zongming Cai, Weishun Fu, Xin Zhang, Fangqiang Sun and Yangquan Li
Minerals 2025, 15(1), 59; https://doi.org/10.3390/min15010059 - 8 Jan 2025
Cited by 7 | Viewed by 1669
Abstract
Acid mine drainage (AMD) generated during the exploitation and utilization of mineral resources poses a severe environmental problem globally within the mining industry. The Xiaomixi Stream in Ziyang County, Shaanxi Province, is a primary tributary of the Han River, which is surrounded by [...] Read more.
Acid mine drainage (AMD) generated during the exploitation and utilization of mineral resources poses a severe environmental problem globally within the mining industry. The Xiaomixi Stream in Ziyang County, Shaanxi Province, is a primary tributary of the Han River, which is surrounded by historically concentrated mining areas for stone coal and vanadium ores. Rainwater erosion of abandoned mine tunnels and waste rock piles has led to the leaching of acidic substances and heavy metals, which then enter the Haoping River and its tributaries through surface runoff. This results in acidic water, posing a significant threat to the water quality of the South-to-North Water Diversion Middle Route within the Han River basin. According to this study’s investigation, Xiaomixi’s acidic water exhibits yellow and white precipitates upstream and downstream of the river, respectively. These precipitates stem from the oxidation of iron-bearing minerals and aluminum-bearing minerals. The precipitation process is controlled by factors such as the pH and temperature, exhibiting seasonal variations. Taking the Xiaomixi Stream in Ziyang County, Shaanxi Province, as the study area, this paper conducts field investigations, systematic sampling of water bodies and river sediments, testing for iron and aluminum pollutants in water, and micro-area observations using field emission scanning electron microscopy (FESEM) on sediments, along with analyzing the iron and aluminum content. The deposition is analyzed using handheld X-ray fluorescence (XRF) analyzers, X-ray diffraction (XRD), and visible–near-infrared spectroscopy data, and a geochemical model is established using PHREEQC software. This paper summarizes the migration and transformation mechanisms of iron and aluminum pollutants in acidic water and proposes appropriate prevention and control measures. Full article
(This article belongs to the Special Issue Acid Mine Drainage: A Challenge or an Opportunity?)
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18 pages, 1369 KB  
Article
A Multidisciplinary Approach for the Assessment of the Last Surviving ‘Marrone di Chiusa Pesio’ Chestnut Trees in the Piemonte Region (Italy)
by Giovanna Neirotti, Alessandro Tomatis, Federica Germanà, Alessia Imparato, Andrea Valisena, Maria Gabriella Mellano, Paola Ruffa, Dario Donno, Daniela Torello Marinoni, Gabriele Loris Beccaro and Giovanni Gamba
Diversity 2024, 16(12), 711; https://doi.org/10.3390/d16120711 - 21 Nov 2024
Viewed by 1333
Abstract
Chestnut orchards are a multifunctional resource, providing not only fruit or wood but also playing a role in the conservation of mountain and hillside landscapes. In the Piemonte Region, Italy, a rich genetic heritage of chestnut genotypes has contributed to considerable biodiversity and [...] Read more.
Chestnut orchards are a multifunctional resource, providing not only fruit or wood but also playing a role in the conservation of mountain and hillside landscapes. In the Piemonte Region, Italy, a rich genetic heritage of chestnut genotypes has contributed to considerable biodiversity and environmental value. The study aimed to valorize an important example of the chestnut agrobiodiversity in the Piemonte Region by focusing on the ‘Marrone di Chiusa Pesio’ (MCP) cultivar (cv). A multidisciplinary approach was applied, involving genetic and morphological analyses, Visual Tree Assessment (VTA), and phytochemical and nutritional profiling. The plant census provided identification and geolocation of 187 MCP specimens; the 20 most representative trees were genetically analyzed, and then, through the VTA, their morpho-functional status was evaluated. The nutraceutical properties and phytochemical composition were assessed by measuring the total polyphenol content (TPC), antioxidant capacity (AOC), and other phytochemical classes through spectrophotometric and chromatographic methods. The results showed significantly higher TPC values (ranged from 36.51 ± 1.60 mgGAE/100 g of dried weight—DW to 103.14 ± 1.24 mgGAE/100 g DW) compared to other ‘Marrone-type’ cultivars, along with high levels of key phenolic markers, bioactive compounds, and nutritional substances. These included tannins (about 22–28 mg/100 g DW) and cinnamic acids (about 23–25 mg/100 g DW), followed by flavonols, benzoic acids, organic acids, monoterpenes, vitamin C, and catechins, listed in order of predominance. A Principal Component Analysis (PCA) was performed to observe the distribution of the samples and their correlations based on the chemical composition. The results confirmed the interesting phytochemical properties of the ‘Marrone di Chiusa Pesio’ nuts, together with their good morphological and functional properties. Given the ongoing genetic erosion of Castanea sativa cultivars, due to cultivation abandonment and climate change, the main factors contributing to the progressive loss of biodiversity worldwide, the presented approach aimed to provide an overview of the conservation status of the local agrobiodiversity. This study highlighted the value of a local chestnut cultivar, presenting the low conservation status of the few remaining specimens. The goal was to define the significant phenotypic variation regarding MCP in the considered area due to environmental variations, which may be of interest in its genetic adaptation to climate change. The study may potentially encourage the development of strategies for actively conserving the forest agrobiodiversity and hillside ecosystem services in the highly diverse landscapes of the Alpine valleys. Full article
(This article belongs to the Special Issue Biodiversity in Italy: Past and Future Perspectives)
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11 pages, 1922 KB  
Article
Association between Oral Microbiome and Gastroesophageal Reflux Severity
by Declan J. Power, Vincent Ho and Jerry Zhou
J. Clin. Med. 2024, 13(15), 4479; https://doi.org/10.3390/jcm13154479 - 31 Jul 2024
Cited by 6 | Viewed by 2833
Abstract
Background/Objectives: Gastroesophageal reflux disease (GORD) is caused by gastric contents refluxing back into the oesophagus and oral cavity. It can lead to injuries to the mucosa in the form of erosion and ulcers. Our past research have shown acid reflux severity and disease [...] Read more.
Background/Objectives: Gastroesophageal reflux disease (GORD) is caused by gastric contents refluxing back into the oesophagus and oral cavity. It can lead to injuries to the mucosa in the form of erosion and ulcers. Our past research have shown acid reflux severity and disease progression is associated with alternations in the microbiota of the distal oesophagus. The aim of this study was to explore whether changes in the oral microbiota occurred in GORD patients and establish any associations with reflux severity. Methods: Fresh mouthwash samples were collected from 58 patients experiencing reflux symptoms referred for 24 h pH monitoring. The participants were categorised into three groups based on their DeMeester scores: Normal (<14.72), Mild (14.2–50), and Moderate/severe (>51). Microorganism identity and diversity were generated using hypervariable tag sequencing and analysing the V1–V3 region of the 16S rRNA gene. Results: No differences in microbiota diversity were found in oral microbiota between groups using the Chiao1 diversity index and Shannon diversity index. Microbiota in the Mild group showed reductions in Rothia dentocariosa and Lautropia, while Moryella and Clostridiales_1 were increased compared with the Normal group. In the Moderate/severe group, the abundance of Rothia aeria was reduced compared with the Normal group, while Schwartzia, Rs_045, Paludibacter, S. satelles, Treponema, and T. socranskii all had increased abundance. The abundance of Prevotella pallens was higher in the Mild group compared with Moderate/severe, while S. satelles and Paludibacter abundances were lower. Conclusions: Our study shows the oral microbiome show significant differences between acid reflux severity groups, as categorised by DeMeester score. Full article
(This article belongs to the Special Issue Clinical Management in Gastroesophageal Reflux)
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24 pages, 990 KB  
Article
Phenotypic Diversity of Pomegranate Cultivars: Discriminating Power of Some Morphological and Fruit Chemical Characteristics
by Mira Radunić, Maja Jukić Špika and Jelena Gadže
Horticulturae 2024, 10(6), 563; https://doi.org/10.3390/horticulturae10060563 - 28 May 2024
Cited by 3 | Viewed by 6549
Abstract
In modern agricultural production, where a small number of commercial cultivars dominate, the collection, evaluation, and preservation of germplasm are important tasks to reduce the erosion of genes and preserve biodiversity. The aim of this study is to characterize the morphological and fruit [...] Read more.
In modern agricultural production, where a small number of commercial cultivars dominate, the collection, evaluation, and preservation of germplasm are important tasks to reduce the erosion of genes and preserve biodiversity. The aim of this study is to characterize the morphological and fruit chemical properties of the pomegranate germplasm grown on the East Adriatic coast, including the commercial cultivars ‘Hicaznar’, ‘Granada’, and ‘Wonderful’, and to highlight the characteristics with the greatest discriminating power. The characterization of the tree, leaf, flower, arils, seed, and juice was carried out using the UPOV descriptor. The colors of the peel, arils, and juice were analyzed according to the CIEL*a*b* method, total soluble solids were measured using refractometers, and total acidity was determined by titration with 0.1 M NaOH. The research results showed significant diversity between the cultivars, which were grouped into several clusters using an unsupervised analysis technique. Factors such as plant vigor, plant growth habit, predominant number of leaves per node on young shoots, crown type, fruit shape, fruit shape in cross-section, peel weight, total aril weight, aril weight, number of arils per fruit, seed length and width, seed yield, total acidity, TSS/TA ratio, and color parameters of the peel, arils, and juice showed high variability, indicating their strong discriminating power in determining the phenotypic diversity of pomegranate. Full article
(This article belongs to the Special Issue Research on Pomegranate Germplasm, Breeding, Genetics and Multiomics)
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15 pages, 1932 KB  
Article
Linkage between Granite Weathering and Gully Erosion in Subtropical Region
by Shu Zhang, Yong Zhang, Gang Huang, Bo Zhang, Yichan Li, Xin Chen, Junkang Xu and Yujie Wei
Water 2024, 16(5), 751; https://doi.org/10.3390/w16050751 - 1 Mar 2024
Cited by 3 | Viewed by 7715
Abstract
Granites, widely distributed in the Earth’s crust, undergo pedogenic processes, shaping diverse soil-mantled landscapes influenced by climatic factors in different regions. Investigating the geochemical signatures in granite weathering profiles across varying climatic conditions provides valuable insights into the intricate interplay between weathering and [...] Read more.
Granites, widely distributed in the Earth’s crust, undergo pedogenic processes, shaping diverse soil-mantled landscapes influenced by climatic factors in different regions. Investigating the geochemical signatures in granite weathering profiles across varying climatic conditions provides valuable insights into the intricate interplay between weathering and landscape evolution. In this study, the geochemical features, particularly major and rare earth elements, and the weathering degree of granites across temperate to subtropical regions in China were examined. The results indicated significant variations in the geochemical characteristics of granite weathering profiles, both at a pedon and regional scale (p < 0.01). With increasing hydrothermal conditions from north to south, soil pH shifted from neutral to acidic, accompanied by the leaching of major elements (K2O, Na2O, CaO, and MgO) and the enrichment of Al and Fe. The total rare earth elements (∑REEs) ranged from 75 to 352 ppm, and light rare earth elements (LREEs) from 71 to 317 ppm, exhibiting less significant variations across the study area, while heavy rare earth elements (HREEs) showed higher concentrations in the subtropical region (3 to 35 ppm). Plagioclases dominated the weathering process in temperate regions, with K-feldspar progressively increasing and, eventually, dominating from temperate to subtropic regions, resulting in a shift in clay minerals from 2:1-type in the temperate to 1:1-type in the subtropic. The chemical index of alteration (CIA) and comprehensive weathering index (W) increased from fresh rock to residual soils along the weathering profiles and from north to south across the study area, ranging from 50.72 to 97.44 and 35.11 to 70.62, respectively. The intensified granite weathering degree was significantly influenced by climatic conditions (p < 0.05), especially the multi-year average precipitation (22.4%) and relative humidity (9.1%) (p < 0.01). Gully erosion on the granite weathering mantle was concentrated in granites with a comprehensive weathering index exceeding 52.51, and the spatial variation of the granite weathering degree aligned with the spatial distribution of gully density across the study area. Full article
(This article belongs to the Special Issue Monitoring and Control of Soil and Water Erosion)
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36 pages, 14281 KB  
Article
Safeguarding Our Heritage—The TRIQUETRA Project Approach
by Charalabos Ioannidis, Styliani Verykokou, Sofia Soile, Denis Istrati, Constantine Spyrakos, Apostolos Sarris, Dimitris Akritidis, Haralambos Feidas, Aristeidis K. Georgoulias, Efstathia Tringa, Prodromos Zanis, Charalampos Georgiadis, Salvatore Martino, Federico Feliziani, Gian Marco Marmoni, Daniele Cerra, Marco Ottinger, Felix Bachofer, Anastasia Anastasiou, Vasiliki (Betty) Charalampopoulou, Patrick Krebs, Boris Mizaikoff, Jean-Christophe Roulet, Xavier Bulliard, Gabriela Dudnik and George C. Anyfantisadd Show full author list remove Hide full author list
Heritage 2024, 7(2), 758-793; https://doi.org/10.3390/heritage7020037 - 5 Feb 2024
Cited by 16 | Viewed by 5689
Abstract
Cultural heritage (CH) sites are frequently exposed to natural elements, and their exposure becomes particularly precarious with the onset of climate change. This increased vulnerability places these sites at risk of deterioration or complete destruction. Risks such as land deformation, floods, acid rain, [...] Read more.
Cultural heritage (CH) sites are frequently exposed to natural elements, and their exposure becomes particularly precarious with the onset of climate change. This increased vulnerability places these sites at risk of deterioration or complete destruction. Risks such as land deformation, floods, acid rain, and erosion significantly threaten historic monuments, while water-related hazards, significantly influenced by both climate change and human activities, present a particularly grave risk to these invaluable sites. Considerable research efforts have focused on safeguarding CH sites. However, there remains a deficiency in systemic approaches towards identifying and mitigating risks for CH sites. The TRIQUETRA project proposes a technological toolbox and a methodological framework for tackling climate change risks and natural hazards threatening CH in the most efficient way possible. It aims at creating an evidence-based assessment platform allowing precise risk stratification as well as a database of available mitigation measures and strategies, acting as a Decision Support System (DSS) towards efficient risk mitigation and site remediation. TRIQUETRA is a European project that brings together a diverse group of researchers with varied expertise, encompassing university research groups, research institutes, public entities, as well as small and medium-sized enterprises. In this article, TRIQUETRAs overall methodology is presented, and preliminary results concerning risk identification, TRIQUETRAs knowledge base, as well as novel sensors and coatings, are discussed. Full article
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18 pages, 4976 KB  
Article
Dynamic Shifts in Soil Fungal Functional Group Characteristics across Distinct Vegetation Types during Ecological Restoration in Degraded Red Soil Regions
by Xiaolong Hou, Junbao Yu, Xuejie Han, Lin Zhai, Taimoor Hassan Farooq, Qiyan Li and Linghua Liu
Forests 2024, 15(1), 89; https://doi.org/10.3390/f15010089 - 2 Jan 2024
Cited by 5 | Viewed by 5929
Abstract
The red soil region in southern China has become the second-largest soil erosion area after the Loess Plateau. The evolutionary trajectory of soil fungi during vegetation restoration in acidic red soil regions remains a subject of inquiry. The investigation focused on the restoration [...] Read more.
The red soil region in southern China has become the second-largest soil erosion area after the Loess Plateau. The evolutionary trajectory of soil fungi during vegetation restoration in acidic red soil regions remains a subject of inquiry. The investigation focused on the restoration process of an ecosystem facing intense degradation in the southern regions of China by studying four distinctive vegetation types: barren land (BL), pure Pinus massoniana forest (CF), mixed coniferous (CBF), and broad-leaved forest (BF). The outcomes revealed considerable enhancements in soil properties’ attributes, evident through a gradual reduction in the bulk density of soil (SBD) and a corresponding increment in soil moisture content (MC), total nitrogen (TN), total carbon (TC), total potassium (TK), soil organic matter (SOM), and available potassium (AK) as vegetation restoration advanced. An intriguing trend emerged where the relative abundance of Ascomycota fungi displayed a declining trajectory, whereas Basidiomycota fungi exhibited an ascending trend with the progression of vegetation restoration. Specifically, broad-leaved forests exhibited a significantly greater relative abundance of Penicillium fungi compared to other stages of vegetation restoration. The diversity of soil fungal communities increased in tandem with vegetation restoration. A redundancy analysis illuminated a strong and positive relationship between the abundance of major soil fungi and soil pH, TN, and TC (key influencers of acidic red soil fungal populations). This study provided additional evidence of an elevation in ectomycorrhizal and saprophytic trophic fungi, signifying a transition that enhances the vegetation’s ability to capture water and nutrients. This, in turn, contributes to the overall enrichment and diversity of vegetation communities during the progression of restoration. Full article
(This article belongs to the Section Forest Soil)
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12 pages, 1021 KB  
Article
Sensory Evaluation, Biochemical, Bioactive and Antioxidant Properties in Fruits of Wild Blackthorn (Prunus spinosa L.) Genotypes from Northeastern Türkiye
by Gulce Ilhan
Horticulturae 2023, 9(9), 1052; https://doi.org/10.3390/horticulturae9091052 - 19 Sep 2023
Cited by 8 | Viewed by 2357
Abstract
Wild edible fruits are an important source for agriculture worldwide suffering from genetic erosion due to a severe genetic diversity reduction and domestication hindrance. In Türkiye, underutilized Prunus spinosa fruits are increasingly being considered as genetic resources and are marginally used by small [...] Read more.
Wild edible fruits are an important source for agriculture worldwide suffering from genetic erosion due to a severe genetic diversity reduction and domestication hindrance. In Türkiye, underutilized Prunus spinosa fruits are increasingly being considered as genetic resources and are marginally used by small farmers constituting a real safety valve for the sustainability of the processing plum value chain. Fruits of those plum genotypes differ in their biometric, processing and functional quality attributes. In this study, fruits of eight wild grown blackthorn (Prunus spinosa) genotypes were sampled from the Ispir district of the Erzurum province and subjected to sensory, morphological, biochemical and antioxidant characterization. Aroma, taste and juiciness were used as the criteria for sensory analysis, and a trained panel of ten experts established and evaluated the sensory characteristics of the fruits of the blackthorn. Fruit weight, fruit skin and flesh color as L*, a* and b* values were the main morphological parameters. For biochemical and bioactive analysis, organic acids, SSC (Soluble Solid Content), vitamin C, total anthocyanins, total phenolic content and total antioxidant capacity were determined. Antioxidant capacity was determined by FRAP (ferric reducing antioxidant power) assay. The results indicated significant differences among genotypes for most of the traits. The fruit weight was found between 2.78–3.67 g. The skin L*, a* and b* values were 13.11–16.12, 2.56–3.85 and 2.01–3.44, respectively. The flesh L*, a* and b* values were in the ranges of 17.45–20.37, 4.88–6.73 and 4.12–5.66, respectively. The SSC content ranged from 18.66% to 21.07%. The total phenolic content (TPC), total anthocyanin content (TAC) and ferric reducing antioxidant power (FRAP) were between 372–504 mg GAE/100 g; 53–72 mg cy-3 g eq./100 g and 107–134 mmol Fe (II) eq./g, respectively. The dominant organic acid was malic acid for all genotypes and varied from 1.04 g/100 g to 1.52 g/100 g fresh weight base. The data showed that the analyzed blackthorns, particularly PS-5, PS-3 and PS-2 had bigger fruits indicate their suitability for fresh and dried consumption, PS-1 and PS-3 had higher juiciness, indicating their suitability for processing, and PS-4 and PS-6 had higher human health promoting compounds (higher total phenolic content and antioxidant capacity), making them suitable for future use as functional foods and as promising sources of natural antioxidants. Full article
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18 pages, 3092 KB  
Article
Designing Sustainable Polymer Blends: Tailoring Mechanical Properties and Degradation Behaviour in PHB/PLA/PCL Blends in a Seawater Environment
by Leonardo G. Engler, Naiara C. Farias, Janaina S. Crespo, Noel M. Gately, Ian Major, Romina Pezzoli and Declan M. Devine
Polymers 2023, 15(13), 2874; https://doi.org/10.3390/polym15132874 - 29 Jun 2023
Cited by 31 | Viewed by 5638
Abstract
Biodegradable polyesters are a popular choice for both packaging and medical device manufacture owing to their ability to break down into harmless components once they have completed their function. However, commonly used polyesters such as poly(hydroxybutyrate) (PHB), poly(lactic acid) (PLA), and polycaprolactone (PCL), [...] Read more.
Biodegradable polyesters are a popular choice for both packaging and medical device manufacture owing to their ability to break down into harmless components once they have completed their function. However, commonly used polyesters such as poly(hydroxybutyrate) (PHB), poly(lactic acid) (PLA), and polycaprolactone (PCL), while readily available and have a relatively low price compared to other biodegradable polyesters, do not meet the degradation profiles required for many applications. As such, this study aimed to determine if the mechanical and degradation properties of biodegradable polymers could be tailored by blending different polymers. The seawater degradation mechanisms were evaluated, revealing surface erosion and bulk degradation in the blends. The extent of degradation was found to be dependent on the specific chemical composition of the polymer and the blend ratio, with degradation occurring via hydrolytic, enzymatic, oxidative, or physical pathways. PLA presents the highest tensile strength (67 MPa); the addition of PHB and PCL increased the flexibility of the samples; however, the tensile strength reduced to 25.5 and 18 MPa for the blends 30/50/20 and 50/25/25, respectively. Additionally, PCL presented weight loss of up to 10 wt.% and PHB of up to 6 wt.%; the seawater degradation in the blends occurs by bulk and surface erosion. The blending process facilitated the flexibility of the blends, enabling their use in diverse industrial applications such as medical devices and packaging. The proposed methodology produced biodegradable blends with tailored properties within a seawater environment. Additionally, further tests that fully track the biodegradation process should be put in place; incorporating compatibilizers might promote the miscibility of different polymers, improving their mechanical properties and biodegradability. Full article
(This article belongs to the Special Issue Environmentally Friendly Bio-Based Polymeric Materials)
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26 pages, 1169 KB  
Article
Cultivating Biodiversity to Harvest Sustainability: Vermicomposting and Inoculation of Microorganisms for Soil Preservation and Resilience
by Maysa Mathias Alves Pereira, Ludmila Caproni Moraes, María Cristina Troya Mogollón, Carlos Junio Falconi Borja, Mateus Duarte, Victor Hugo Teixeira Buttrós, José Magno Queiroz Luz, Moacir Pasqual and Joyce Dória
Agronomy 2023, 13(1), 103; https://doi.org/10.3390/agronomy13010103 - 28 Dec 2022
Cited by 19 | Viewed by 5990
Abstract
Based on the concepts of circular economy and bioeconomy, the reuse of agrifood residues through vermicomposting can help solve serious environmental problems such as soil contamination and degradation, erosion and climate change. In this sense, the objective was to identify, quantify and analyze [...] Read more.
Based on the concepts of circular economy and bioeconomy, the reuse of agrifood residues through vermicomposting can help solve serious environmental problems such as soil contamination and degradation, erosion and climate change. In this sense, the objective was to identify, quantify and analyze the physical, chemical, hormonal, amino acid content and microbial biodiversity of three formulations of vermicompost, with and without inoculation of microorganisms from native forest and commercial formulation, aiming at the production of an organic fertilizer rich in microorganisms for use in sustainable production systems. As a result, the vermicompost formulations presented values higher than the minimum requirements stipulated by Brazilian legislation for the registration of class A composite organic fertilizer. There is a significant difference between the vermicomposts, in the parameters related to the content of phosphorus, auxin, tryptophan and organic matter, as well as the relation between humic and fulvic acids. Bacillus sp. and Trichoderma sp. were also influenced by the type of vermicompost formulation. In addition, inoculation with microorganisms from native forest promoted an increase in biodiversity, in which the presence of Actinomyces sp. and Azotobacter chrooccocum contribute to the reduction in the levels of heavy metals in the compost. It is concluded that vermicomposting is a potential tool in the reuse of agri-food residues, with expressive microbial diversity that can influence plant growth, suppression of pathogens, minimize or reduce the effects of biotic and abiotic stresses on plant production, in addition to contributing to maintenance of soil biodiversity, integral fertility and resilience to climate change. Full article
(This article belongs to the Special Issue Applications of Soil Microorganisms for Sustainable Crop Production)
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Article
DNA Barcoding and ITS2 Secondary Structure Predictions in Taro (Colocasia esculenta L. Schott) from the North Eastern Hill Region of India
by Mayengbam Premi Devi, Madhumita Dasgupta, Sansuta Mohanty, Susheel Kumar Sharma, Vivek Hegde, Subhra Saikat Roy, Rennya Renadevan, Kinathi Bipin Kumar, Hitendra Kumar Patel and Manas Ranjan Sahoo
Genes 2022, 13(12), 2294; https://doi.org/10.3390/genes13122294 - 5 Dec 2022
Cited by 6 | Viewed by 3516
Abstract
Taro (Colocasia esculenta L. Schott, Araceae), an ancient root and tuber crop, is highly polygenic, polyphyletic, and polygeographic in nature, which leads to its rapid genetic erosion. To prevent the perceived loss of taro diversity, species discrimination and genetic conservation of [...] Read more.
Taro (Colocasia esculenta L. Schott, Araceae), an ancient root and tuber crop, is highly polygenic, polyphyletic, and polygeographic in nature, which leads to its rapid genetic erosion. To prevent the perceived loss of taro diversity, species discrimination and genetic conservation of promising taro genotypes need special attention. Reports on genetic discrimination of taro at its center of origin are still untapped. We performed DNA barcoding of twenty promising genotypes of taro indigenous to the northeastern hill region of India, deploying two chloroplast-plastid genes, matK and rbcL, and the ribosomal nuclear gene ITS2. The secondary structure of ITS2 was determined and molecular phylogeny was performed to assess genetic discrimination among the taro genotypes. The matK and rbcL genes were highly efficient (>90%) in amplification and sequencing. However, the ITS2 barcode region achieved significant discrimination among the tested taro genotypes. All the taro genotypes displayed most similar sequences at the conserved matK and rbcL loci. However, distinct sequence lengths were observed in the ITS2 barcode region, revealing accurate discriminations among the genotypes. Multiple barcode markers are unrelated to one another and change independently, providing different estimations of heritable traits and genetic lineages; thus, they are advantageous over a single locus in genetic discrimination studies. A dynamic programming algorithm that used base-pairing interactions within a single nucleic acid polymer or between two polymers transformed the secondary structures into the symbol code data to predict seven different minimum free energy secondary structures. Our analysis strengthens the potential of the ITS2 gene as a potent DNA barcode candidate in the prediction of a valuable secondary structure that would help in genetic discrimination between the genotypes while augmenting future breeding strategies in taro. Full article
(This article belongs to the Special Issue Phylogenetics, Genetics, and Breeding of Medicinal Plants)
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