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26 pages, 14606 KB  
Review
Attribution-Based Explainability in Medical Imaging: A Critical Review on Explainable Computer Vision (X-CV) Techniques and Their Applications in Medical AI
by Kazi Nabiul Alam, Pooneh Bagheri Zadeh and Akbar Sheikh-Akbari
Electronics 2025, 14(15), 3024; https://doi.org/10.3390/electronics14153024 - 29 Jul 2025
Cited by 8 | Viewed by 6248
Abstract
One of the largest future applications of computer vision is in the healthcare industry. Computer vision tasks are generally implemented in diverse medical imaging scenarios, including detecting or classifying diseases, predicting potential disease progression, analyzing cancer data for advancing future research, and conducting [...] Read more.
One of the largest future applications of computer vision is in the healthcare industry. Computer vision tasks are generally implemented in diverse medical imaging scenarios, including detecting or classifying diseases, predicting potential disease progression, analyzing cancer data for advancing future research, and conducting genetic analysis for personalized medicine. However, a critical drawback of using Computer Vision (CV) approaches is their limited reliability and transparency. Clinicians and patients must comprehend the rationale behind predictions or results to ensure trust and ethical deployment in clinical settings. This demonstrates the adoption of the idea of Explainable Computer Vision (X-CV), which enhances vision-relative interpretability. Among various methodologies, attribution-based approaches are widely employed by researchers to explain medical imaging outputs by identifying influential features. This article solely aims to explore how attribution-based X-CV methods work in medical imaging, what they are good for in real-world use, and what their main limitations are. This study evaluates X-CV techniques by conducting a thorough review of relevant reports, peer-reviewed journals, and methodological approaches to obtain an adequate understanding of attribution-based approaches. It explores how these techniques tackle computational complexity issues, improve diagnostic accuracy and aid clinical decision-making processes. This article intends to present a path that generalizes the concept of trustworthiness towards AI-based healthcare solutions. Full article
(This article belongs to the Special Issue Artificial Intelligence-Driven Emerging Applications)
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21 pages, 2138 KB  
Article
Biological Control of Tomato Bacterial Leaf Spots and Its Impact on Some Antioxidant Enzymes, Phenolic Compounds, and Pigment Content
by Asmaa H. Akila, Mohamed A. S. Ali, Ahmed M. Khairy, Ahmed S. M. Elnahal, Haifa E. Alfassam, Hassan A. Rudayni, Fatima A. Jaber and Mohamed R. A. Tohamy
Biology 2024, 13(6), 369; https://doi.org/10.3390/biology13060369 - 23 May 2024
Cited by 18 | Viewed by 6813
Abstract
Tomato bacterial spots, caused by Xanthomonas campestris pv. vesicatoria (Xcv1) and X. euvesicatoria (Xe2), as well as bacterial specks, caused by two strains of Pseudomonas syringae pv. tomato (Pst1 and Pst2), represent significant threats to tomato production [...] Read more.
Tomato bacterial spots, caused by Xanthomonas campestris pv. vesicatoria (Xcv1) and X. euvesicatoria (Xe2), as well as bacterial specks, caused by two strains of Pseudomonas syringae pv. tomato (Pst1 and Pst2), represent significant threats to tomato production in the El-Sharkia governorate, often resulting in substantial yield losses. The objective of this study was to evaluate the efficacy of various biocontrol culture filtrates, including bacteria and fungi agents, in managing the occurrence and severity of these diseases, while also monitoring physiological changes in tomato leaves, including antioxidant enzymes, phenolics, and pigment content. The culture filtrates from examined Trichoderma species (T. viride, T. harzianum, and T. album), as well as the tested bacteria (Bacillus subtilis, Pseudomonas fluorescens, and Serratia marcescens) at concentrations of 25%, 50%, and 100%, significantly inhibited the proliferation of pathogenic bacteria In vitro. For the In vivo experiments, we used specific doses of 5 mL of spore suspension per plant for the fungal bioagents at a concentration of 2.5 × 107 spores/mL. The bacterial bioagents were applied as a 10 mL suspension per plant at a concentration of 1 × 108 CFU/mL. Spraying the culture filtrates of the tested bioagents two days before infection In vivo significantly reduced disease incidence and severity. Trichoderma viride exhibited the highest efficacy among the fungal bioagents, followed by T. harzianum and T. album. Meanwhile, the culture filtrate of B. subtilis emerged as the most potent among the bacterial bioagents, followed by P. fluorescens. Furthermore, applying these culture filtrates resulted in elevated levels of chitinase, peroxidase, and polyphenol oxidase activity. This effect extended to increased phenol contents, as well as chlorophyll a, chlorophyll b, and carotenoids in sprayed tomato plants compared to the control treatment. Overall, these findings underscore the potential of these biocontrol strategies to effectively mitigate disease incidence and severity while enhancing plant defense mechanisms and physiological parameters, thus offering promising avenues for sustainable disease management in tomato production. Full article
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13 pages, 2350 KB  
Article
The Identification of Bee Comb Cell Contents Using Semiconductor Gas Sensors
by Beata Bąk, Jakub Wilk, Piotr Artiemjew, Maciej Siuda and Jerzy Wilde
Sensors 2023, 23(24), 9811; https://doi.org/10.3390/s23249811 - 14 Dec 2023
Cited by 3 | Viewed by 2232
Abstract
Beekeeping is an extremely difficult field of agriculture. It requires efficient management of the bee nest so that the bee colony can develop efficiently and produce as much honey and other bee products as possible. The beekeeper, therefore, must constantly monitor the contents [...] Read more.
Beekeeping is an extremely difficult field of agriculture. It requires efficient management of the bee nest so that the bee colony can develop efficiently and produce as much honey and other bee products as possible. The beekeeper, therefore, must constantly monitor the contents of the bee comb. At the University of Warmia and Mazury in Olsztyn, research is being carried out to develop methods for efficient management of the apiary. One of our research goals was to test whether a gas detector (MCA-8) based on six semiconductor sensors—TGS823, TGS826, TGS832, TGS2600, TGS2602, and TGS2603 from the company FIGARO—is able to recognize the contents of bee comb cells. For this purpose, polystyrene and wooden test chambers were created, in which fragments of bee comb with different contents were placed. Gas samples were analyzed from an empty comb, a comb with sealed brood, a comb with open brood, a comb with carbohydrate food in the form of sugar syrup, and a comb with bee bread. In addition, a sample of gas from an empty chamber was tested. The results in two variants were analyzed: (1) Variant 1, the value of 270 s of sensor readings from the sample measurement (exposure phase), and (2) Variant 2, the value of 270 s of sensor readings from the sample measurement (measurement phase) with baseline correction by subtracting the last 600 s of surrounding air measurements (flushing phase). A five-time cross-validation 2 (5xCV2) test and the Monte Carlo cross-validation 25 (trained and tested 25 times) were performed. Fourteen classifiers were tested. The naive Bayes classifier (NB) proved to be the most effective method for distinguishing individual classes from others. The MCA-8 device brilliantly differentiates an empty comb from a comb with contents. It differentiates better between an empty comb and a comb with brood, with results of more than 83%. Lower class accuracy was obtained when distinguishing an empty comb from a comb with food and a comb with bee bread, with results of less than 73%. The matrix of six TGS sensors in the device shows promising versatility in distinguishing between various types of brood and food found in bee comb cells. This capability, though still developing, positions the MCA-8 device as a potentially invaluable tool for enhancing the efficiency and effectiveness of beekeepers in the future. Full article
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28 pages, 5492 KB  
Article
Implementation of MEC-Assisted Collective Perception in an Integrated Artery/Simu5G Simulation Framework
by Gergely Attila Kovács and László Bokor
Sensors 2023, 23(18), 7968; https://doi.org/10.3390/s23187968 - 19 Sep 2023
Cited by 8 | Viewed by 3824
Abstract
Advanced vehicle-to-everything (V2X) safety applications must operate with ultra-low latency and be highly reliable. Therefore, they require sophisticated supporting technologies. This is especially true for cooperative applications, such as Collective Perception (CP), where a large amount of data constantly flows among vehicles and [...] Read more.
Advanced vehicle-to-everything (V2X) safety applications must operate with ultra-low latency and be highly reliable. Therefore, they require sophisticated supporting technologies. This is especially true for cooperative applications, such as Collective Perception (CP), where a large amount of data constantly flows among vehicles and between vehicles and a network intelligence server. Both low and high-level support is needed for such an operation, meaning that various access technologies and other architectural elements also need to incorporate features enabling the effective use of V2X applications with strict requirements. The new 5G core architecture promises even more supporting technologies, like Multi-access Edge Computing (MEC). To test the performance of these technologies, an integrated framework for V2X simulations with 5G network elements is proposed in the form of combining Simu5G, a standalone 5G implementation, with the go-to V2X-simulator, Artery. As a first step toward a fully functional MEC-assisted CP Service, an extension to Simu5G’s edge implementation is introduced. The edge application is responsible for dispatching the Collective Perception Messages generated by the vehicles via the 5G connectivity so that a MEC server provided by the network can process incoming data. Simulation results prove the operability of the proposed integrated system and edge computing’s potential in assisting V2X scenarios. Full article
(This article belongs to the Special Issue The Intelligent Sensing Technology of Transportation System)
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16 pages, 5662 KB  
Article
Identification of Key Transcription Factors Related to Bacterial Spot Resistance in Pepper through Regulatory Network Analyses
by Qingquan Zhu, Shenghua Gao and Wenli Zhang
Genes 2021, 12(9), 1351; https://doi.org/10.3390/genes12091351 - 29 Aug 2021
Cited by 9 | Viewed by 4969
Abstract
Bacterial spot (BS), caused by Xanthomonas campestris pv. Vesicatoria (Xcv), severely affects the quality and yield of pepper. Thus, breeding new pepper cultivars with enhanced resistance to BS can improve economic benefits for pepper production. Identification of BS resistance genes is [...] Read more.
Bacterial spot (BS), caused by Xanthomonas campestris pv. Vesicatoria (Xcv), severely affects the quality and yield of pepper. Thus, breeding new pepper cultivars with enhanced resistance to BS can improve economic benefits for pepper production. Identification of BS resistance genes is an essential step to achieve this goal. However, very few BS resistance genes have been well characterized in pepper so far. In this study, we reanalyzed public multiple time points related to RNA-seq data sets from two pepper cultivars, the Xcv-susceptible cultivar ECW and the Xcv-resistant cultivar VI037601, post Xcv infection. We identified a total of 3568 differentially expressed genes (DEGs) between two cultivars post Xcv infection, which were mainly involved in some biological processes, such as Gene Ontology (GO) terms related to defense response to bacterium, immune system process, and regulation of defense response, etc. Through weighted gene co-expression network analysis (WGCNA), we identified 15 hub (Hub) transcription factor (TF) candidates in response to Xcv infection. We further selected 20 TFs from the gene regulatory network (GRN) potentially involved in Xcv resistance response. Finally, we predicted 4 TFs, C3H (p-coumarate 3-hydroxylase), ERF (ethylene-responsive element binding factor), TALE (three-amino-acid-loop-extension), and HSF (heat shock transcription factor), as key factors responsible for BS disease resistance in pepper. In conclusion, our study provides valuable resources for dissecting the underlying molecular mechanism responsible for Xcv resistance in pepper. Additionally, it also provides valuable references for mining transcriptomic data to identify key candidates for disease resistance in horticulture crops. Full article
(This article belongs to the Section Plant Genetics and Genomics)
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