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An aerobic, obligately chemolithoautotrophic, sulfur-oxidizing bacterium, strain AK1^T, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka, Russia. The cells of the new isolate were Gram-negative motile rods with a single polar flagellum. Strain AK1^T grew at 37–55 °C (optimum 50 °C) with 0–1.0% NaCl (optimum 0%) and within the pH range 4.8–7.0 (optimum pH 5.2–5.5). The new isolate was able to grow by aerobic respiration with sulfide, sulfur, or thiosulfate as the electron donor and HCO₃⁻/CO₂ as the carbon source. The major fatty acids were C16:0, C17:1 Δ, and C16:1 ω7c. The respiratory lipoquinone was ubiquinone UQ-8. The size of the genome and genomic DNA G+C content of the strain AK1^T were 2.55 Mb and 64.0%, respectively. The closest 16S rRNA gene sequence of a validly published species belonged to Thiobacter subterraneus C55^T (97.94% identity). According to the 16S rRNA gene sequence-based and conserved protein sequences-based phylogenetic analyses, strain AK1^T represented a distinct lineage of the genus Thiobacter within a new family, Thiobacteraceae of the order Burkholderiales. As inferred from the morphology, physiology, chemotaxonomy, and phylogeny, strain AK1^T ought to be recognized as a novel species for which we propose the name Thiobacter aerophilum sp. nov. The type strain is AK1^T (=CGMCC 1.18099^T = UQM 41819^T). Full article

Over the last half-century, microbial communities of the Kamchatka hot springs have been largely studied using molecular, radioisotopic, and cultural approaches. Generally, these results were obtained for mixed samples of water with sediments, for only hydrothermal water, or for only sediment samples. Simultaneous comparative analysis of the microbial communities of water and sediments was performed for only one Kamchatka hot spring with circumneutral pH. Here, the microbial communities of both sediments and water (separately) of hot spring #4229 (the Uzon Caldera, Kamchatka) with a temperature of 50–56 °C and pH of 3.2 were analyzed by 16S rRNA gene V4 fragment amplicon sequencing. It was revealed that the microbial community of sediments was represented by uncultured phylogenetically deep-branching lineages of archaea, such as ARK-15 within Thermoplasmatota and ‘Ca. Marsarchaeales’ from the Thermoproteota phyla. Metagenome analysis showed that these archaea most probably carried out the degradation of organic matter. The microbial community of the hot water is represented by thermoacidophilic, (micro)aerobic, chemolithoautotrophic, hydrogen- and sulfur-oxidizing bacteria of the genera Hydrogenobaculum (phylum Aquificota) and Acidithiobacillus (phylum Pseudomonadota). Radioisotopic tracing and DNA-stable-isotope probing techniques proved their role as primary producers in the hot spring. The experiment revealed significant differences in the composition and functions of the microbial communities of sediments and water through the example of a typical acidic hot spring in Kamchatka. Full article

The biochemical pathways of anaerobic sulfur disproportionation are only partially deciphered, and the mechanisms involved in the first step of S⁰-disproportionation remain unknown. Here, we present the results of sequencing and analysis of the complete genome of Dissulfurimicrobium hydrothermale strain Sh68^T, one of two strains isolated to date known to grow exclusively by anaerobic disproportionation of inorganic sulfur compounds. Dissulfurimicrobium hydrothermale Sh68^T is a motile, thermophilic, anaerobic, chemolithoautotrophic microorganism isolated from a hydrothermal pond at Uzon caldera, Kamchatka, Russia. It is able to produce energy and grow by disproportionation of elemental sulfur, sulfite and thiosulfate. Its genome consists of a circular chromosome of 2,025,450 base pairs, has a G + C content of 49.66% and a completion of 97.6%. Genomic data suggest that CO₂ assimilation is carried out by the Wood–Ljungdhal pathway and that central anabolism involves the gluconeogenesis pathway. The genome of strain Sh68^T encodes the complete gene set of the dissimilatory sulfate reduction pathway, some of which are likely to be involved in sulfur disproportionation. A short sequence protein of unknown function present in the genome of strain Sh68^T is conserved in the genomes of a large panel of other S⁰-disproportionating bacteria and was absent from the genomes of microorganisms incapable of elemental sulfur disproportionation. We propose that this protein may be involved in the first step of elemental sulfur disproportionation, as S⁰ is poorly soluble and unable to cross the cytoplasmic membrane in this form. Full article

Here, we report on a new geyser (named Shaman) formed in the Uzon caldera (Kronotsky Federal Nature Biosphere Reserve, Russia) in autumn 2008 from a cycling hot Na-Cl spring. The geyser is a pool-type CO₂-gas lift driven. From 2012 to 2018, the geyser has shown a rather stable interval between eruptions (IBE) from 129 to 144 min with a fountain height up to 4 m, and the geyser conduit has gradually enlarged. In 2019, the Shaman geyser eruption mode significantly changed: cold water inflow from the adjacent stream was re-directed into the geyser conduit and the average IBE decreased to 80 min. We observed two eruptive modes: a cycling hot spring (June 2019) and a cycling geyser (after June 2019). Bottom-hole temperature recording was performed in the geyser conduit to understand its activity. The TOUGH2-EOS2 model was used to reproduce the obtained temperature records and estimate geyser recharge/discharge parameters in both modes. Modeling shows that a larger cold inflow into the conduit causes a switch from cycling geyser to hot cycling spring mode. It was also found that the switch to cycling geyser mode corresponds to a larger mass of CO₂ release during the time of the eruption. Full article